BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,567,608	Δ1 bp	74.0%	coding (500/1227 nt)	eutH ←	ethanolamine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,607	0	A	.	74.0%	304.5 / 179.1	154	coding (501/1227 nt)	eutH	ethanolamine transporter
Reads supporting (aligned to +/- strand): ref base A (20/19); new base . (58/56); total (78/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 156 total aligned reads displayed.

TCATGTTCATCAGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACAC  >  NC_000913/2567510‑2567702

tCATGTTCATCAGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCatc                                                                                               >  2:2442478/1‑98 (MQ=255)

  aTGTTCATCAGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCatcgg                                                                                             <  2:929859/100‑5 (MQ=255)

   tGTTGATCAGGATCAGGGTGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATc                                                                                              <  2:3950426/98‑1 (MQ=255)

      tcatcaGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAAt                                                                                         >  1:3230068/1‑100 (MQ=255)

      tcatcaGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATg                                                                                        <  1:1568518/100‑1 (MQ=255)

       catcaGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACGTAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATgg                                                                                       >  2:2261210/1‑100 (MQ=255)

       catcaGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATgg                                                                                       <  1:1984971/100‑1 (MQ=255)

         tcaggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGt                                                                                      >  1:2769126/1‑100 (MQ=255)

         tcaggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTc                                                                                     <  1:2442493/100‑1 (MQ=255)

         tcaggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTc                                                                                     >  2:3608967/1‑100 (MQ=255)

          caggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTc                                                                                     <  2:1656899/100‑1 (MQ=255)

          caggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTca                                                                                    >  1:811710/1‑100 (MQ=255)

           aggatcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTcac                                                                                   <  1:1607584/100‑1 (MQ=255)

              atcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAAt                                                                                <  2:3245826/100‑1 (MQ=255)

               tcaggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAAt                                                                                <  1:2442147/100‑1 (MQ=255)

                caggGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGc                                                                              <  1:1926323/100‑1 (MQ=255)

                 agtgCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGcc                                                                             <  2:2389362/97‑1 (MQ=255)

                  gggCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGccc                                                                            >  2:139552/1‑100 (MQ=255)

                   ggCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCg                                                                           >  2:3903474/1‑100 (MQ=255)

                    gCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGc                                                                          <  1:3110455/100‑1 (MQ=255)

                      naaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCa                                                                        >  1:1308942/2‑100 (MQ=255)

                      gAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGcc                                                                         >  1:3787299/1‑100 (MQ=255)

                       aaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCa                                                                        <  2:3406733/100‑1 (MQ=255)

                       aaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAg                                                                       <  1:2338141/100‑1 (MQ=255)

                       aaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAg                                                                       <  2:251592/100‑1 (MQ=255)

                       aaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAg                                                                       <  2:1836924/100‑1 (MQ=255)

                       aaaGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAg                                                                       >  1:3264153/1‑100 (MQ=255)

                        aggttaaTTGCACCGGGTGGCCGGTGATCGGCACACCGGAGTACATAGCAACCAGACCGCCAGCGAGACAA‑CGATCGGAAGGGTCACAATGCCCGCCAg                                                                       <  2:3733801/94‑1 (MQ=255)

                          gTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCAc                                                                    >  2:10271/1‑100 (MQ=255)

                            gAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACg                                                                   <  1:2850414/100‑1 (MQ=255)

                            gAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGc                                                                  >  2:4130526/1‑100 (MQ=255)

                             aaTTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGcc                                                                 <  1:213442/100‑1 (MQ=255)

                             aaTTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGcc                                                                 >  1:1960562/1‑100 (MQ=255)

                             aaTTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGcc                                                                 <  1:589969/100‑1 (MQ=255)

                              aTTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCg                                                                >  2:59533/1‑100 (MQ=255)

                              aTTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAACGCCCGCCAGCACGCCg                                                                >  2:985266/1‑100 (MQ=255)

                                 ccACCGGCTGGCCGTTGATCTGCACACCGGCGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGCCACAATGCCCGCCAGCACGCCGAgc                                                             >  2:896582/1‑100 (MQ=255)

                                 ccACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAg                                                              <  2:2400257/100‑1 (MQ=255)

                                 ccACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAgc                                                             <  2:803756/100‑1 (MQ=255)

                                  cACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAgcg                                                            >  2:1527622/1‑100 (MQ=255)

                                     cGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATAAGAATGGGCACAATGCCCGCCAGCACGCCGAgcgc                                                           >  2:3816526/1‑99 (MQ=255)

                                       gCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGa                                                       <  1:1013547/100‑1 (MQ=255)

                                        cTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGAAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGAt                                                      >  2:2957511/1‑100 (MQ=255)

                                            ccGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAac                                                   <  2:4045486/100‑1 (MQ=255)

                                              gTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAacgac                                                <  1:3882802/100‑1 (MQ=255)

                                               ttGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAacgacg                                               <  2:1630734/100‑1 (MQ=255)

                                                tGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGCATGGTCACAATGCCCGCCAGCACACCGAGAGCCAGATCacggc                                                >  2:3364075/1‑97 (MQ=255)

                                                tGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACgg                                              <  1:10272/100‑1 (MQ=255)

                                                 gATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACgg                                              <  2:298761/99‑1 (MQ=255)

                                                 gATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGt                                             <  2:2867491/100‑1 (MQ=255)

                                                  aTCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTc                                            <  2:500851/100‑1 (MQ=255)

                                                  aTCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTc                                            <  2:921678/100‑1 (MQ=255)

                                                     tGCACATCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACACCGCCCGCCAGCACGCCAAGCGCCAGATAACGACGGTCAGa                                         >  2:3960664/1‑100 (MQ=255)

                                                     tGCACACCGGAGTACATAGCCCCCAGACCCCCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCa                                           <  1:1701952/98‑1 (MQ=255)

                                                      gCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGaa                                        >  1:1254500/1‑100 (MQ=255)

                                                       cacaCCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAg                                       >  1:3363078/1‑100 (MQ=255)

                                                       cacaCCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAg                                       <  2:1121658/100‑1 (MQ=255)

                                                        acacCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAAGGACGGTCAGAAgg                                      <  2:2574062/100‑1 (MQ=255)

                                                         nacCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAgg                                      >  2:3287575/2‑100 (MQ=255)

                                                         cacCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAgg                                      >  1:1269772/1‑100 (MQ=255)

                                                         cacCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGt                                     >  1:164876/1‑100 (MQ=255)

                                                         cacCGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGt                                     >  2:4144244/1‑100 (MQ=255)

                                                           ccGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATCACGACGGTCAGAAGGTTc                                   >  2:705270/1‑100 (MQ=255)

                                                           ccGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTc                                   >  2:1709819/1‑100 (MQ=255)

                                                            cGGAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCg                                  <  2:1367150/100‑1 (MQ=255)

                                                            cGGAGTACAGAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACCATGCACGGCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTc                                   <  2:1007510/99‑1 (MQ=255)

                                                             ggAGTACATCGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAAAGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCg                                  <  2:3435174/99‑1 (MQ=255)

                                                             ggAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGa                                 <  1:1876187/100‑1 (MQ=255)

                                                             ggAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGa                                 >  1:3370210/1‑100 (MQ=255)

                                                              gAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGAt                                <  1:2282123/100‑1 (MQ=255)

                                                              gAGTACATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGAt                                <  1:2960768/100‑1 (MQ=255)

                                                               aGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACACTGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGAt                                <  1:682046/100‑1 (MQ=255)

                                                               aGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGAt                                <  2:3845373/100‑1 (MQ=255)

                                                                gTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATa                               <  2:882866/100‑1 (MQ=255)

                                                                  aCATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAAt                             >  2:2045059/1‑100 (MQ=255)

                                                                   cATAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGc                           >  2:2233509/1‑100 (MQ=255)

                                                                     tAGCAACCAGACCACCAGCGATACAACC‑ATGGGCATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCg                         >  2:2741264/1‑100 (MQ=255)

                                                                     tAGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCg                         >  2:1258139/1‑100 (MQ=255)

                                                                      aGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCg                         >  1:3599783/1‑100 (MQ=255)

                                                                      aGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCg                         <  2:1833296/100‑1 (MQ=255)

                                                                      aGCAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGa                        >  2:259134/1‑100 (MQ=255)

                                                                        cAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAg                       <  1:703034/100‑1 (MQ=255)

                                                                        cAACCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGTCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAgc                      <  2:674373/100‑1 (MQ=255)

                                                                         aaCCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAgcg                     <  1:2090767/100‑1 (MQ=255)

                                                                         aaCCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAgag                     >  1:4101801/1‑98 (MQ=255)

                                                                          aCCAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAgcgc                    >  1:2717638/1‑100 (MQ=255)

                                                                            cAGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCa                  >  2:3590931/1‑100 (MQ=255)

                                                                             aGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCa                  >  1:1516581/1‑100 (MQ=255)

                                                                             aGACCACCAGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCAc                 >  2:1090581/1‑100 (MQ=255)

                                                                                ccaccaGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGCAGGTTCGATAATGCCGAGCGCCACCgg              >  2:3167008/1‑100 (MQ=255)

                                                                                ccaccaGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCgg              >  1:2246616/1‑100 (MQ=255)

                                                                                ccaccaGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCgg              >  1:536089/1‑100 (MQ=255)

                                                                                    caGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATg          <  1:1124267/100‑1 (MQ=255)

                                                                                    caGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATg          <  1:677653/100‑1 (MQ=255)

                                                                                     aGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGCAGGTTCGATAATGCCGAGCGCCACCGGAATgg         >  2:4101329/1‑100 (MQ=255)

                                                                                     aGCGATACAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATgg         <  1:4082079/100‑1 (MQ=255)

                                                                                       cGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGa        <  1:986353/100‑1 (MQ=255)

                                                                                           aCAACC‑ATTGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGaaa      <  2:36593/97‑1 (MQ=255)

                                                                                           aCAACC‑ATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAaca   <  1:2984006/100‑1 (MQ=255)

                                                                                            cAACC‑ATCGGAATGGTCACAATGCCCGCCAACACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAacac  >  1:3149618/1‑100 (MQ=255)

TCATGTTCATCAGGATCAGGGCGAAAGTGAATTCCACCGGCTGGCCGTTGATCTGCACACCGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACAC  >  NC_000913/2567510‑2567702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`