Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,450,608 (T)6→5 100% coding (742/2274 nt) tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,450,6030T.68.5% 179.5 / 174.0 124coding (747/2274 nt)tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base . (43/42);  total (61/63)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACAC  >  NC_000913/1450504‑1450701
                                                                                                   |                                                                                                  
aCCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACt                                                                                                    >  1:3520907/1‑100 (MQ=255)
   gACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttt                                                                                                 <  1:3791908/100‑1 (MQ=255)
   gACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttt                                                                                                 <  1:1596387/100‑1 (MQ=255)
     cATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttc                                                                                               >  1:1611861/1‑99 (MQ=255)
      aTCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttcg                                                                                              <  2:3629528/100‑3 (MQ=255)
       tCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttcgc                                                                                             >  1:3040196/1‑97 (MQ=255)
       tCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttcgc                                                                                             >  1:833783/1‑97 (MQ=255)
        cAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACtttttcgca                                                                                            <  1:921617/100‑5 (MQ=255)
         aaGATAGCTGATGACTTTGAGCAACCGGGCTGCTTGTTTCAGGCTATCTTTACCATCGAAATCGCTTACGGTCAGCGGCGTGGTAATCACTTTTTCGCAt                                                                                          >  2:3539264/1‑100 (MQ=255)
            aTAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAg                                                                                       <  2:2624560/100‑1 (MQ=255)
             tAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGt                                                                                      >  2:1205528/1‑100 (MQ=255)
                 tgatgaCTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATa                                                                                  >  2:4056175/1‑100 (MQ=255)
                  gatgaCTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATAc                                                                                 >  2:3959640/1‑100 (MQ=255)
                    tgaCTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATAc                                                                                 <  2:721067/98‑1 (MQ=255)
                     gaCTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACcgc                                                                              >  2:446674/1‑100 (MQ=255)
                      aCTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACcgcg                                                                             >  1:2302816/1‑100 (MQ=255)
                       cTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGt                                                                            >  1:1540388/1‑100 (MQ=255)
                       cTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGt                                                                            >  1:460478/1‑100 (MQ=255)
                       cTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGt                                                                            >  2:1056155/1‑100 (MQ=255)
                        tttGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGtt                                                                           <  2:715211/100‑1 (MQ=255)
                         ttGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGtt                                                                           <  2:3200851/100‑1 (MQ=255)
                           gAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTttct                                                                        <  1:1886746/100‑1 (MQ=255)
                             gCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTttcttc                                                                      >  1:2332085/1‑100 (MQ=255)
                             gCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTttcttc                                                                      <  1:3549343/100‑1 (MQ=255)
                              cAACCGGGCATCTTGTTTCAGGCCATCTTTACCTTTGAAATAACCTACTGTCATCGGCGTGGTAATTTCTTTTTCGCATCAGTAATACCGCGTttct                                                                        <  2:2643055/97‑1 (MQ=255)
                              cAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCa                                                                     >  2:3097687/1‑100 (MQ=255)
                               aaCCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCAc                                                                    >  2:1422151/1‑100 (MQ=255)
                                 ccGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACg                                                                   <  1:2655346/100‑1 (MQ=255)
                                       aTCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCgg                                                            <  1:3664913/100‑1 (MQ=255)
                                       aTCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCgg                                                            >  1:3652512/1‑100 (MQ=255)
                                       aTCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCgg                                                            <  2:3589415/100‑1 (MQ=255)
                                       aTCATGTTTCAGGCCATCATTACCATCGAAATAACCTACGGTCAGCTGCGTTGTAACCACTTTTTTCGTATCATTAATATCTCGTTTCTTCCCGGCAGCg                                                             <  2:2239154/100‑1 (MQ=255)
                                        tCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGc                                                           >  2:3007948/1‑100 (MQ=255)
                                        tCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGc                                                           >  2:548875/1‑100 (MQ=255)
                                         cTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGc                                                           >  1:251589/1‑100 (MQ=255)
                                         cTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCa                                                          <  1:3226475/100‑1 (MQ=255)
                                          ttGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCaa                                                         >  1:2714637/1‑100 (MQ=255)
                                              ttCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGGGTTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttc                                                     >  1:1230520/1‑100 (MQ=255)
                                               tCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttct                                                    <  1:2725492/100‑1 (MQ=255)
                                               tCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttct                                                    >  1:196537/1‑100 (MQ=255)
                                                cAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttctt                                                   <  2:3977888/100‑1 (MQ=255)
                                                 aGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttctt                                                   >  1:2755924/1‑100 (MQ=255)
                                                 aGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCTCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttcttc                                                  >  2:3689750/1‑100 (MQ=255)
                                                 aGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttcttc                                                  <  1:3299094/100‑1 (MQ=255)
                                                  ggCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttcttc                                                  <  1:596565/100‑1 (MQ=255)
                                                   gCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCAc                                                >  1:1016862/1‑100 (MQ=255)
                                                   gCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCAc                                                <  1:520926/100‑1 (MQ=255)
                                                   gCCATCTTTACCATCGAAATAACCTACCGTCAGCGGCGTAGTAATCACTTTTTGCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAAttcttc                                                  <  2:2056695/99‑1 (MQ=255)
                                                    ccATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCAc                                                >  2:3386459/1‑100 (MQ=255)
                                                      aTCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACtgt                                             <  1:298313/100‑1 (MQ=255)
                                                       tCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACtgtt                                            <  2:1609847/100‑1 (MQ=255)
                                                         tttACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACtgttgt                                          <  2:3067912/100‑1 (MQ=255)
                                                          ttACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACtgttgt                                          <  2:3405318/100‑1 (MQ=255)
                                                          ttACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTgttgtt                                         >  2:1518832/1‑100 (MQ=255)
                                                          ttACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTgttgtt                                         <  1:1441255/100‑1 (MQ=255)
                                                          taaCCATCGAAATAACCTACGGTAAGCGGCGTGGTAAGCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAACTTCTTCACtgttgt                                          <  2:2701755/97‑1 (MQ=255)
                                                           tACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTGCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACtgttg                                           <  2:3644545/98‑1 (MQ=255)
                                                           tACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTta                                        <  1:3002157/100‑1 (MQ=255)
                                                              cATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaa                                       >  1:1343410/1‑99 (MQ=255)
                                                              cATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaata                                     >  2:2557855/1‑100 (MQ=255)
                                                              cATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaata                                     >  1:2713328/1‑100 (MQ=255)
                                                              cATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaata                                     <  1:2557870/100‑1 (MQ=255)
                                                               aTCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaataa                                    >  2:51252/1‑100 (MQ=255)
                                                               aTCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTtaataa                                    <  1:1193820/100‑1 (MQ=255)
                                                               aTCGAAATAAACTACGGTCAGCGGCGTGGTACTCCCATTTTCGCATCAGTACTACAGCGTATCTTCAAGAACCCGGCAACTTCTTCACTATTGTtaat                                      >  2:175955/1‑98 (MQ=255)
                                                                  gAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGt                                 >  1:2334980/1‑100 (MQ=255)
                                                                  gAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGt                                 <  2:3495261/100‑1 (MQ=255)
                                                                   aaaTAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATg                                  <  2:3412475/98‑1 (MQ=255)
                                                                    aaTAACCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTc                               <  1:3012203/100‑1 (MQ=255)
                                                                       aaCCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  1:1925424/100‑1 (MQ=255)
                                                                       aaCCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  2:1709524/100‑1 (MQ=255)
                                                                       aaCCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  2:3285244/100‑1 (MQ=255)
                                                                       aaCCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  1:1205533/100‑1 (MQ=255)
                                                                       aaCCTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  2:3009139/100‑1 (MQ=255)
                                                                        aCCTACGGTCAGCGGCGTGGTAATCACTTTTGCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGca                           <  2:2006971/100‑1 (MQ=255)
                                                                         ccTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGca                           <  1:60958/100‑1 (MQ=255)
                                                                         ccTACGGTCAGCGGCGTGGTAATCACTTTTTTTTTTTTTTTTTACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCt                              >  2:1407390/1‑96 (MQ=255)
                                                                         ccTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGcac                          >  1:960181/1‑100 (MQ=255)
                                                                          cTACGGTCAGCGGCGTGGTAATCACTTTTTCGCATCATTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGc                            <  1:4044376/97‑1 (MQ=255)
                                                                           tACGGTCAGTGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAACGGCAACTTCTTCACTGTTGTTAATAATGTTCTGCacac                        >  2:2926064/1‑100 (MQ=255)
                                                                            aCGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGACAAATTCTTCACTGTTGTTAATAATGTTCTGCACACt                       <  1:1014090/100‑1 (MQ=255)
                                                                             cGGTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTg                      >  1:1058330/1‑100 (MQ=255)
                                                                              ggTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTg                      >  1:373799/1‑100 (MQ=255)
                                                                              ggTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTgg                     >  2:2949772/1‑100 (MQ=255)
                                                                              ggTCAGCGGCGTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTgg                     <  2:1690762/100‑1 (MQ=255)
                                                                               gTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTgg                     >  1:4129523/1‑100 (MQ=255)
                                                                                      gcgTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAatc              >  1:2752354/1‑100 (MQ=255)
                                                                                       cgTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAatca             <  2:1215434/100‑1 (MQ=255)
                                                                                        gTGGTAATCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAatcatc           >  1:3560206/1‑100 (MQ=255)
                                                                                         tGGTAATCACTTGTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATcc          <  2:4083925/100‑1 (MQ=255)
                                                                                          ggTAATCTCTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATcc          <  2:3072537/100‑1 (MQ=255)
                                                                                          ggTAATCAATTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATcc          <  2:498270/100‑1 (MQ=255)
                                                                                          ggTAATCATGTCTTCGCATCAGGAACACCGCGTTGCTTCACTGCAGAGGCAAATTCTTCTCTGTTGTTAGTAATGGTCTGCACACTGGCGAAATCATCCa         <  2:2992222/100‑1 (MQ=255)
                                                                                           gTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCa         >  2:3883050/1‑100 (MQ=255)
                                                                                            tAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAg        <  1:2994819/100‑1 (MQ=255)
                                                                                             aaTCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGc       >  2:1453733/1‑100 (MQ=255)
                                                                                             aaTCACTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCa      >  1:336675/1‑100 (MQ=255)
                                                                                              aTCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCa      >  1:3355706/1‑100 (MQ=255)
                                                                                                 aCTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAaca   >  1:988730/1‑100 (MQ=255)
                                                                                                  cTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAacac  >  1:1377221/1‑100 (MQ=255)
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ACCGACATCAAGATAGCTGATGACTTTGAGCAACCGGGCATCTTGTTTCAGGCCATCTTTACCATCGAAATAACCTACGGTCAGCGGCGTGGTAATCACTTTTTTCGCATCAGTAATACCGCGTTTCTTCACGGCAGCGGCAAATTCTTCACTGTTGTTAATAATGTTCTGCACACTGGCGAAATCATCCAGCAACAC  >  NC_000913/1450504‑1450701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: