Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,555 Δ1 bp 16.2% coding (1126/1452 nt) uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,5550G.16.2% 401.9 / 78.6 136coding (1126/1452 nt)uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (61/52);  new base . (12/10);  total (73/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 144 total aligned reads displayed.

TGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAC  >  NC_000913/1609457‑1609654
                                                                                                  |                                                                                                    
tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     <  1:3297852/100‑1 (MQ=255)
tGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagc                                                                                                     >  1:1604560/1‑100 (MQ=255)
 gattCGCCGTTACGCTCACTACGATAGAACGCAATCAATGCTGCTATTGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCcagc                                                                                                     >  2:3396776/3‑99 (MQ=255)
  tttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcagcag                                                                                                   >  1:754119/1‑100 (MQ=255)
   ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCagcagc                                                                                                  >  2:3008324/1‑100 (MQ=255)
    tCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCt                                                                                                 >  1:2395553/1‑100 (MQ=255)
    tCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCt                                                                                                 >  2:3688932/1‑100 (MQ=255)
     cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                >  2:2865945/1‑100 (MQ=255)
     cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                >  1:3327993/1‑100 (MQ=255)
      gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGGCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                <  2:3543555/99‑1 (MQ=255)
      gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgt                                                                                              >  1:1280462/1‑100 (MQ=255)
       ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                             <  1:1566092/100‑1 (MQ=255)
        cGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTgg                                                                                            >  1:3942808/1‑100 (MQ=255)
         gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtgag                                                                                           >  1:4053365/1‑98 (MQ=255)
         gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                           <  2:3346396/100‑1 (MQ=255)
         gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                           >  1:3151538/1‑100 (MQ=255)
          ttACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTTGCa                                                                                          <  1:1863895/100‑1 (MQ=255)
           tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                         <  2:915655/100‑1 (MQ=255)
           tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                         <  1:2321455/100‑1 (MQ=255)
            aCGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAgg                                                                                        >  2:1219936/1‑100 (MQ=255)
             cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                       <  1:69420/100‑2 (MQ=255)
             cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                       <  1:2276107/100‑2 (MQ=255)
             cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGCGTGCCGTTTGCCTTCTGCCCTCCCACAGCtgctcgcagg                                                                                        <  2:3506411/99‑9 (MQ=255)
              gCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggc                                                                                      <  1:2491023/100‑3 (MQ=255)
              gCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggc                                                                                      <  1:2714614/100‑3 (MQ=255)
               cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATg                                                                                     >  2:2905465/1‑100 (MQ=255)
               cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                     <  1:3453607/100‑4 (MQ=255)
               cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                     <  1:2855142/100‑4 (MQ=255)
                  ttccgatctgAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCgg                                                                                  <  1:3702741/91‑1 (MQ=255)
                   ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                 <  2:603795/100‑1 (MQ=255)
                   ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                 >  1:3127896/1‑100 (MQ=255)
                   ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                 <  2:1426450/100‑1 (MQ=255)
                    cacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCATCTGCCCGGACACAGCTGCTGGCAgggcagaagg                                                                                <  2:492152/100‑9 (MQ=255)
                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGt                                                                                >  1:3566280/1‑99 (MQ=255)
                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                               <  1:2429697/100‑1 (MQ=255)
                     acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                               <  1:50356/100‑1 (MQ=255)
                      cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGc                                                                              <  2:1182581/100‑1 (MQ=255)
                       gATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGGGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCg                                                                             <  2:4031435/100‑1 (MQ=255)
                          aGAACGCAATCAATGACGCTCATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCGTGCCAGCAGCTGTGGCTGGATGCGGGTGCg                                                                             >  2:3451363/1‑97 (MQ=255)
                           gAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggagatcggaa                                                                         >  2:3702714/1‑91 (MQ=255)
                             aCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                       <  1:1975059/100‑1 (MQ=255)
                             aCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                       >  2:1605838/1‑100 (MQ=255)
                               gCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                     <  1:3844774/100‑1 (MQ=255)
                                caatcaatGTCGCTCATGCGACAGTAAGGCGCGCAGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGTTTGCGGGTGCTGAACTTgtt                                                                    <  2:3477183/100‑3 (MQ=255)
                                caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                    >  2:3126373/1‑100 (MQ=255)
                                caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                    <  1:3097165/100‑1 (MQ=255)
                                 aatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTc                                                                   <  1:808541/100‑1 (MQ=255)
                                         ccGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGtt                                                           <  2:2870088/100‑1 (MQ=255)
                                          cGCCAATGCGAAAGTCAGGCGCGCCGGAAGTGTGCTGTCTGCCTTCTGCCATGCCAGCGGCTGTGGGAGGGTGCGGGTGCGGGAGTTGGTCATACCGTTg                                                          <  2:1015975/100‑1 (MQ=255)
                                                tgggAAAGTGAGGCGCGCCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                    <  2:1060460/97‑1 (MQ=255)
                                                 gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                   >  2:1904000/1‑100 (MQ=255)
                                                  cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  >  2:3006580/1‑100 (MQ=255)
                                                  cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                  <  1:1880439/100‑1 (MQ=255)
                                                  cGAAAGTAAGCCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                    >  2:1831059/1‑98 (MQ=255)
                                                     aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGa                                               >  1:562207/1‑100 (MQ=255)
                                                        tAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcag                                            >  1:3660243/1‑100 (MQ=255)
                                                          aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                          >  1:1362314/1‑100 (MQ=255)
                                                          aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                          <  2:754115/100‑1 (MQ=255)
                                                           ggCGCGCGGGAAGTGTGCCGTTTGCCATCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                         >  1:2627184/1‑100 (MQ=255)
                                                           ggCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                         <  1:2521957/100‑1 (MQ=255)
                                                            gcgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACagcagc                                        >  1:2730389/1‑100 (MQ=255)
                                                                gcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGAt                                    <  1:601270/100‑1 (MQ=255)
                                                                 ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                   >  1:3924848/1‑100 (MQ=255)
                                                                 ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                   <  2:1939007/100‑1 (MQ=255)
                                                                  cGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGc                                  <  1:1807547/100‑1 (MQ=255)
                                                                   ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                 >  1:2180401/1‑100 (MQ=255)
                                                                   ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                 <  1:1670720/100‑1 (MQ=255)
                                                                      aGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTaa                              >  2:3979247/1‑100 (MQ=255)
                                                                        tgtgCCGGTTGCGGTCGGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCGTACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATg                            <  2:1311108/100‑1 (MQ=255)
                                                                         gtgCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGt                           >  1:2488007/1‑100 (MQ=255)
                                                                            ccGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACg                        >  1:1707717/1‑100 (MQ=255)
                                                                              gTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                      <  1:2009738/100‑1 (MQ=255)
                                                                              gTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                      <  1:925734/100‑1 (MQ=255)
                                                                                  gCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTAc                  <  2:220684/100‑1 (MQ=255)
                                                                                   ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTatg                 >  2:2520319/1‑98 (MQ=255)
                                                                                   ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                 >  2:2744834/1‑100 (MQ=255)
                                                                                    cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                >  2:4172249/8‑100 (MQ=38)
                                                                                    cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                >  1:1295777/8‑100 (MQ=38)
                                                                                    cTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                >  2:2903825/1‑100 (MQ=255)
                                                                                     ttCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa               <  1:751301/100‑1 (MQ=255)
                                                                                     ttCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa               >  2:4112139/1‑100 (MQ=255)
                                                                                       gcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa             >  1:2903189/5‑100 (MQ=255)
                                                                                       gcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa             >  1:2071292/5‑100 (MQ=255)
                                                                                       ctgccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTAGGGGTTACGGaaa             <  1:2745622/100‑1 (MQ=255)
                                                                                        catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc            >  2:2374614/4‑100 (MQ=255)
                                                                                        catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc            >  1:1538058/4‑100 (MQ=255)
                                                                                        catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc            >  1:3518079/4‑100 (MQ=255)
                                                                                        catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc            >  1:3884247/4‑100 (MQ=255)
                                                                                         gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  2:1129441/1‑100 (MQ=255)
                                                                                         gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           <  1:2987888/100‑1 (MQ=255)
                                                                                         gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCCTAATGTACGGGTTACGGAAACg           <  2:2421176/100‑1 (MQ=255)
                                                                                         gccctaccAGCAGCTGTGGCAGGATGCGGGTGCGGAAGTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  2:2223474/1‑100 (MQ=255)
                                                                                         atcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg           >  1:1067241/3‑100 (MQ=255)
                                                                                          ccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGc          <  1:2325833/100‑1 (MQ=255)
                                                                                           cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc         >  1:3678369/1‑100 (MQ=255)
                                                                                             tgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCg       <  2:3924818/100‑1 (MQ=255)
                                                                                              gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg      <  2:1759504/100‑1 (MQ=255)
                                                                                              gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg      >  1:3817789/1‑100 (MQ=255)
                                                                                                cagcagCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTg    <  2:2466646/100‑1 (MQ=255)
                                                                                                  gcagcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc  >  1:2957535/1‑100 (MQ=255)
                                                                                                  |                                                                                                    
TGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAC  >  NC_000913/1609457‑1609654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: