Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,101,510 (A)8→7 100% pseudogene (235/349 nt) wbbL ← pseudogene, lipopolysaccharide biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,101,5030A.72.9% 236.8 / 158.5 129pseudogene (242/349 nt)wbbLpseudogene, lipopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (14/21);  new base . (44/50);  total (58/71)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 143 total aligned reads displayed.

GAAAAACTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACA  >  NC_000913/2101404‑2101598
                                                                                                   |                                                                                               
gAAAAACTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGa                                                                                                 >  2:1429573/1‑100 (MQ=255)
 aaaaaCTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaa                                                                                                >  2:277749/1‑100 (MQ=255)
  aaacCTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaa                                                                                               <  1:2266085/100‑1 (MQ=255)
  aaaaCTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaa                                                                                               >  1:4066707/1‑100 (MQ=255)
   aaaCTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaa                                                                                              <  1:1674082/100‑1 (MQ=255)
    aaCTGATGAAATTCGATCAAAGTTGCGATTTGATTAAATACGTTTTCTGGCTAAATATCTTAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaa                                                                                              <  1:1476175/99‑1 (MQ=255)
     aCTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaa                                                                                            <  1:3947319/100‑1 (MQ=255)
      cTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaa                                                                                           <  1:2721257/100‑1 (MQ=255)
      cTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaa                                                                                           <  2:1658079/100‑1 (MQ=255)
         atgaAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaactt                                                                                        >  1:3779938/1‑97 (MQ=255)
         atgaAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACt                                                                                        >  1:3227680/1‑100 (MQ=255)
          tgaAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaacttc                                                                                       >  2:127658/1‑96 (MQ=255)
          tgaAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaacttc                                                                                       <  1:2631954/100‑5 (MQ=255)
          tgaAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGaaaaaaacttc                                                                                       >  1:2106916/1‑96 (MQ=255)
               ttCGATCAAAGTTGCTATTTGATAAGAGACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGAtt                                                                                  <  2:1833008/99‑1 (MQ=255)
               ttCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGAtt                                                                                  <  1:3982445/100‑1 (MQ=255)
                  gATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAACAAAACTTCGATTGTCa                                                                              >  2:2438152/1‑100 (MQ=255)
                  gATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCa                                                                              >  1:2534581/1‑100 (MQ=255)
                   aTCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCat                                                                             <  1:1737710/100‑1 (MQ=255)
                   aTCAAAGTTGCGATGTGATAAAATACGTTTTCTGGCTAAATATATAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCat                                                                             <  2:3621454/100‑1 (MQ=255)
                     cAAAGTTGCAATTTGACAAAATACGTGTGCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCatga                                                                           <  2:2468132/100‑1 (MQ=255)
                       aaGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCatgatg                                                                         <  2:3566188/100‑1 (MQ=255)
                        aGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCAtgatga                                                                        <  2:4015111/100‑1 (MQ=255)
                         gTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAg                                                                       <  2:3061239/100‑1 (MQ=255)
                         gTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAg                                                                       <  1:664637/100‑1 (MQ=255)
                           tGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCa                                                                     <  1:1266031/100‑1 (MQ=255)
                            gCGATTTGATAAAATACGTTTTCTGGCTAAATATTTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATTATGAGCa                                                                     <  2:2190631/100‑1 (MQ=255)
                             cGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCAt                                                                    >  1:606254/1‑100 (MQ=255)
                             cGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATa                                                                   >  1:3724559/1‑100 (MQ=255)
                                tttGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAAt                                                                 <  1:2644178/100‑1 (MQ=255)
                                  tGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTa                                                              >  1:486028/1‑100 (MQ=255)
                                  tGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTa                                                              <  1:2118812/100‑1 (MQ=255)
                                  tGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTa                                                              >  1:637966/1‑100 (MQ=255)
                                   gATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTAt                                                             >  1:2717983/1‑100 (MQ=255)
                                    aTAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTTTCATGATGAGCATAATGTATc                                                            >  2:3488539/1‑100 (MQ=255)
                                    aTAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATc                                                            >  1:446892/1‑100 (MQ=255)
                                     tAAAATACGTTTTCTGTCTAAATATCTAAAAGCACTTTTTAAGTGACATCTGCAGGCTTTTGACAAAAACTTCGATTGTCATGCTGAGCATAATGTATCg                                                           <  2:2428505/100‑1 (MQ=255)
                                     tAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATc                                                            >  1:325532/1‑100 (MQ=255)
                                      agaacaCGTTTTCTAGCTAAATATCTAATAGTACTTTTTAAGTGCCATCTGGAGGCTTTTGAAAAAATCTTCGATGGTCATGATGAGCATAATGTATCGc                                                          <  2:2239881/95‑1 (MQ=255)
                                      aaaaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATc                                                            >  2:841621/1‑99 (MQ=255)
                                      aaaaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGc                                                          <  2:857711/100‑1 (MQ=255)
                                       aaaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGc                                                          <  2:2719470/100‑1 (MQ=255)
                                        aaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCAt                                                        >  1:3530401/1‑100 (MQ=255)
                                        aaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCAt                                                        >  2:3307688/1‑100 (MQ=255)
                                        aaTACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCCTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCAt                                                        >  2:1107187/1‑100 (MQ=255)
                                          tACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGa                                                      <  2:3778819/100‑1 (MQ=255)
                                            cGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGaaa                                                    >  2:1969109/1‑100 (MQ=255)
                                            cGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGaaa                                                    <  1:934804/100‑1 (MQ=255)
                                             gTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGaaaa                                                   >  1:813481/1‑100 (MQ=255)
                                              ttttCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAg                                                  >  1:1028485/1‑100 (MQ=255)
                                              ttttCTGAGTAAATATTTAAGAGTACTTTTTAAATGCCATCTGAAGGCTTTTGAATTTATCTTCATTTTTCATCATGAGCCTAGCTTATCGCATGaaa                                                    <  2:2571555/98‑1 (MQ=255)
                                                ttCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCg                                                <  1:979023/100‑1 (MQ=255)
                                                  cTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCggg                                              <  1:3481714/100‑1 (MQ=255)
                                                   tGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAACCTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGa                                             <  2:3374064/100‑1 (MQ=255)
                                                   tGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGa                                             <  2:205518/100‑1 (MQ=255)
                                                    ggCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGaa                                            >  2:531888/1‑100 (MQ=255)
                                                     gCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAAc                                           >  2:1156129/1‑100 (MQ=255)
                                                     gCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAAc                                           >  1:3579865/1‑100 (MQ=255)
                                                     gCTAAATATCTAAAAGTACTTTTGAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCggg                                              <  2:3408015/97‑1 (MQ=255)
                                                      cTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAAc                                           <  1:1429756/100‑1 (MQ=255)
                                                      cTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACa                                          <  1:1969119/100‑1 (MQ=255)
                                                      cTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACa                                          <  1:2836911/100‑1 (MQ=255)
                                                       tAAATATCTAAGAGTACTTTTTAAGTGCCAGCGTAAGGGTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAAc                                           <  2:3532373/98‑1 (MQ=255)
                                                           tatCTAAAAGTATTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATg                                     <  2:3190713/100‑1 (MQ=255)
                                                           tatCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAAt                                      <  1:527273/100‑1 (MQ=255)
                                                            atCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGa                                    >  2:1419868/1‑100 (MQ=255)
                                                            atCTAAAAGTACTTTTTAAGTGCCATCTGAAGGATAATAAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATg                                     <  2:2471030/100‑1 (MQ=255)
                                                              cTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGaa                                   >  1:2212571/1‑100 (MQ=255)
                                                               tAAAAGTACTTTTTTAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGt                                 <  2:2843190/100‑1 (MQ=255)
                                                               tAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGt                                 >  2:2904290/1‑100 (MQ=255)
                                                               tAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGt                                 >  1:909668/1‑100 (MQ=255)
                                                               tAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGt                                 >  2:1084370/1‑100 (MQ=255)
                                                                aaaaGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTc                                <  1:1091824/100‑1 (MQ=255)
                                                                  aaGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTg                              <  1:797120/100‑1 (MQ=255)
                                                                  aaGTACTTTTTAAGTGCCATCTGAAGGCCTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTg                              <  1:1107192/100‑1 (MQ=255)
                                                                    gTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGa                             >  1:1564961/1‑100 (MQ=255)
                                                                     tACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGac                            <  1:2909355/100‑1 (MQ=255)
                                                                     tACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGaca                           <  2:446889/100‑1 (MQ=255)
                                                                       cTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcc                         >  1:2515268/1‑100 (MQ=255)
                                                                       cTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcc                         >  2:3639508/1‑100 (MQ=255)
                                                                       cTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcc                         >  1:1906389/1‑100 (MQ=255)
                                                                       cTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcc                         >  1:562806/1‑100 (MQ=255)
                                                                       cTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcc                         >  1:3129720/1‑100 (MQ=255)
                                                                         ttttAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAcca                        >  2:3324199/1‑100 (MQ=255)
                                                                         ttttAAGTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAccag                       >  1:2075905/1‑100 (MQ=255)
                                                                         ttttAAGTGCCATCGGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGaca                           <  2:3251113/96‑1 (MQ=255)
                                                                              aGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACAccagccag                   >  2:3217227/1‑100 (MQ=255)
                                                                               gTGCCATCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGc                 <  2:3007791/100‑1 (MQ=255)
                                                                                 gctaTCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCCTAATGTATCGCATGAAAAGCGGAAACATAATGAAGTCTGACACCAGCCAGGCTa               >  2:495446/4‑100 (MQ=255)
                                                                                   cATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTaa              >  1:1286766/1‑100 (MQ=255)
                                                                                     tCTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGcc           <  1:3612145/100‑1 (MQ=255)
                                                                                     tCTGAAGACTATAAAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTa               <  2:3733827/97‑1 (MQ=255)
                                                                                      cTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGcc           <  1:3995106/100‑1 (MQ=255)
                                                                                      cTGAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCt          >  2:2153431/1‑100 (MQ=255)
                                                                                        gAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCa        <  1:4037876/100‑1 (MQ=255)
                                                                                        gAAGGCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCa        <  2:4066676/100‑1 (MQ=255)
                                                                                           ggCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGc     >  2:402511/1‑100 (MQ=255)
                                                                                           ggCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGc     >  1:3849696/1‑100 (MQ=255)
                                                                                           ggCTTTTGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGc     >  2:1258052/1‑100 (MQ=255)
                                                                                              ttttGAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGcaca  >  2:3994830/1‑100 (MQ=255)
                                                                                                   |                                                                                               
GAAAAACTGATGAAATTCGATCAAAGTTGCGATTTGATAAAATACGTTTTCTGGCTAAATATCTAAAAGTACTTTTTAAGTGCCATCTGAAGGCTTTTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACA  >  NC_000913/2101404‑2101598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: