Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,469,656:1 (T)7→8 100% coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,6491.T98.9% 636.9 / ‑0.8 188coding (519/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base T (77/109);  total (79/109)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 217 total aligned reads displayed.

AATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469550‑2469743
                                                                                                    |                                                                                              
aaTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTa                                                                                                 <  1:719035/100‑1 (MQ=255)
aaTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTa                                                                                                 >  1:312722/1‑100 (MQ=255)
 aTGTTTGCATCATTCTATCAAGAGCAAATAATTATACTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTa                                                                                                 <  2:2820702/99‑1 (MQ=255)
  tGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtt                                                                                              >  2:3386058/1‑100 (MQ=255)
    tttGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttt                                                                                            >  1:1078740/1‑100 (MQ=255)
     ttGCATCATTATATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATCCACAAACAATAAATCTATGCTTTTGCTAttttt                                                                                           <  2:2746343/100‑1 (MQ=255)
      tGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttt                                                                                          >  2:3141100/1‑100 (MQ=255)
       gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  2:2865338/1‑100 (MQ=255)
       gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  1:880681/1‑100 (MQ=255)
       gCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttt                                                                                         >  1:2939665/1‑100 (MQ=255)
        catcatTCTATCGAGAGTCAATAGTTATAATTTCCCTTCCATTGTTGGTGCATTTTTTACTATGCAATTTCAATATATCCATCCTTTTGCTAttttttt                                                                                         <  2:3885642/99‑1 (MQ=255)
        catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                        >  1:3808540/1‑99 (MQ=255)
        catcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttt                                                                                        >  1:3379498/1‑99 (MQ=255)
          tcatTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcg                                                                                      >  1:645293/1‑97 (MQ=255)
            atTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttttttcgtt                                                                                    <  2:571365/100‑6 (MQ=255)
             ttCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTg                                                                                    >  1:1124498/1‑100 (MQ=255)
              tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   >  2:1549008/1‑100 (MQ=255)
              tCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGc                                                                                   <  1:1759531/100‑1 (MQ=255)
               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  <  2:880676/100‑1 (MQ=255)
               cTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCt                                                                                  <  1:692670/100‑1 (MQ=255)
                tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCta                                                                                 <  2:116210/100‑1 (MQ=255)
                tATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAtttttttttcgttgc                                                                                 >  1:278840/1‑91 (MQ=255)
                 aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  2:608926/1‑100 (MQ=255)
                 aTCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaa                                                                                >  2:2633748/1‑100 (MQ=255)
                  tCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaat                                                                               <  2:588535/100‑1 (MQ=255)
                   cAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaat                                                                               >  2:1707219/1‑99 (MQ=255)
                    aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtagatc                                                                             >  1:574416/1‑96 (MQ=255)
                    aaGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCtaataa                                                                             >  1:4034270/1‑100 (MQ=255)
                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            <  1:1387576/100‑1 (MQ=255)
                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  2:2056753/1‑100 (MQ=255)
                     agagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTaataat                                                                            >  2:490311/1‑100 (MQ=255)
                      gagCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAataata                                                                           <  1:620672/100‑1 (MQ=255)
                        gCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATc                                                                         <  1:2241607/100‑1 (MQ=255)
                           aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                      <  1:2611366/100‑1 (MQ=255)
                           aaTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTAc                                                                      <  1:3421801/100‑1 (MQ=255)
                            aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:3356910/100‑1 (MQ=255)
                            aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  2:1765870/100‑1 (MQ=255)
                            aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:3717217/100‑1 (MQ=255)
                            aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:1266699/100‑1 (MQ=255)
                            aTAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACt                                                                     <  1:2232409/100‑1 (MQ=255)
                             tAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTg                                                                    <  1:763339/100‑1 (MQ=255)
                              aGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGc                                                                   >  1:1712939/1‑100 (MQ=255)
                                 tataATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaa                                                                >  1:477474/1‑100 (MQ=255)
                                   taATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTaaaa                                                              >  1:91800/1‑100 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  2:2213976/100‑1 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:1165782/100‑1 (MQ=255)
                                     aTTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAAt                                                            <  1:165380/100‑1 (MQ=255)
                                           cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      >  1:3622381/1‑100 (MQ=255)
                                           cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      >  1:3904469/1‑100 (MQ=255)
                                           cttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACGGCTAAAAATCAAttt                                                      >  1:4114216/1‑100 (MQ=255)
                                            tttcATTTTTGGTGTATTTATTAACATGCAAAAACAATAAAACTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAAttt                                                      <  2:1084433/96‑1 (MQ=255)
                                            ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     >  1:1960356/1‑100 (MQ=255)
                                            ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     <  2:3607442/100‑1 (MQ=255)
                                            ttccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     >  1:1697899/1‑100 (MQ=255)
                                            ttccATTTTTGGTGTATTAATAAAAATGAAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTta                                                     <  2:1323762/100‑1 (MQ=255)
                                             tccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtat                                                    >  1:3517569/1‑100 (MQ=255)
                                              ccATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTtata                                                   <  1:3004329/100‑1 (MQ=255)
                                               cATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTatat                                                  >  1:2901015/1‑100 (MQ=255)
                                                  ttttGGTGTATTCATTAACATGCAAAAACAATAATTCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTaa                                               <  1:240633/100‑1 (MQ=255)
                                                  ttttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTaa                                               <  1:2311798/100‑1 (MQ=255)
                                                    ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:706814/100‑1 (MQ=255)
                                                    ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             >  1:3344747/1‑100 (MQ=255)
                                                    ttGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  1:2202814/100‑1 (MQ=255)
                                                     tGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcc                                             <  2:3861051/99‑1 (MQ=255)
                                                       gtttATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAAcccc                                           <  1:3658586/96‑1 (MQ=255)
                                                           aTTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTaa                                      >  1:602825/1‑100 (MQ=255)
                                                            ttCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     <  1:1020614/100‑1 (MQ=255)
                                                            ttCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATATACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     >  1:4039593/1‑100 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTATTATATCTACTGCTAAAAATTAATTTATTTTTACCTCTCTATTa                                    >  2:2347896/1‑99 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTATTAATATTTAATGCTAAAAATCAATTTATATTAACTCTACTAATat                                   >  2:1774025/1‑99 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAAt                                     >  2:3327103/1‑98 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  1:3222760/100‑1 (MQ=255)
                                                              cATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAg                                   <  2:321982/100‑1 (MQ=255)
                                                                ttAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTTCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 >  2:1972194/1‑100 (MQ=255)
                                                                 tAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAgtg                                 <  1:3890254/99‑1 (MQ=255)
                                                                  aaCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTa                               <  1:1549017/100‑1 (MQ=255)
                                                                  aaCATGCAAAAACAATAAATCAATGCTTTTGCTATTTTTTTTCGTTGCTAATCATAACTTCTGCTAAATATCATTTTATATTAAcct                                            >  2:3905439/1‑86 (MQ=255)
                                                                   aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                              <  2:230496/100‑1 (MQ=255)
                                                                   aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                              >  2:775671/1‑100 (MQ=255)
                                                                   aCATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAt                              >  1:3291330/1‑100 (MQ=255)
                                                                    cATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTAtt                             >  1:183709/1‑100 (MQ=255)
                                                                        cAAAAACAATAAATCTATGCTTTTGCTATTTTTTTTTTTTTTTTTTAATATCTACTGCTAAAAATTTATTTTTATTTTTCTCACTAATAGTTTTTTtata                         >  2:4092838/1‑100 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatat                        <  2:2145649/100‑1 (MQ=255)
                                                                         aaaaaCAATAAATCTATGCTTTTGCTATTTTTTTTCGTTACTAATAATATCTACTGCTAAAAATCAATTTTTATTAACCCCACTAATAGTGTATTCatat                        >  2:3441774/1‑100 (MQ=255)
                                                                              cAATAAATCTATGCTTTTGCTATTTTTTTTTGTTTCTAATAATATCTACTGCTAAAAATCAATTTTTATTAACCCCACTAATAATGTATTCatatt                       >  2:162738/1‑96 (MQ=255)
                                                                              cAATAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatat                   >  2:3988342/1‑100 (MQ=255)
                                                                                 tAAATCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATTAATTTATATTAACCACACTAATAGTGTATTCATATTATAtttt                >  2:4106063/1‑100 (MQ=255)
                                                                                    ntCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt             >  2:3953083/2‑100 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  1:2584569/100‑1 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  1:3115572/100‑1 (MQ=255)
                                                                                     tCTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttt            <  1:2056763/100‑1 (MQ=255)
                                                                                      cTATGCTTTTTCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  2:1858668/100‑1 (MQ=255)
                                                                                      cTATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTg           <  2:2793334/100‑1 (MQ=255)
                                                                                       tATGCTTTTGCTATTTTTTTTTTTTTTTTTTTATTTCTACTGCTAAAAATCAATTTTTATTTATCCCACTAATAGTGTTTTCATATTTTAtttttttt            >  2:2441891/1‑98 (MQ=255)
                                                                                       tATGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGa          <  2:3766517/100‑1 (MQ=255)
                                                                                         tGCTTTTGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATa        <  1:2563587/100‑1 (MQ=255)
                                                                                            ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGTTAAAAATCAATTTATATTAATCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac     <  2:3790352/100‑1 (MQ=255)
                                                                                            ttttGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac     <  1:4160417/100‑1 (MQ=255)
                                                                                            ttttGATATTTTTTTTCGTTTCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGac     <  2:3068267/100‑1 (MQ=255)
                                                                                               tGCTATTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGAcaca  >  1:2643978/1‑100 (MQ=255)
                                                                                                    |                                                                                              
AATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACA  >  NC_000913/2469550‑2469743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: