Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,563,518:1 (A)5→6 100% intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,563,5131.A95.6% 460.1 / 12.5 137intergenic (+396/‑79)yffP/yffQCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (4/2);  new base A (60/71);  total (64/73)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 150 total aligned reads displayed.

AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC  >  NC_000913/2563414‑2563606
                                                                                                      |                                                                                              
  gTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaa                                                                                              >  1:505741/1‑100 (MQ=255)
   tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaa                                                                                             >  1:3562276/1‑100 (MQ=255)
   tGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaa                                                                                              >  2:3805127/1‑99 (MQ=255)
        tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  2:1301792/100‑4 (MQ=255)
        tttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatg                                                                                        <  2:2951522/100‑4 (MQ=255)
         ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatga                                                                                       <  1:1667874/100‑5 (MQ=255)
         ttGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaaatga                                                                                       <  2:3659401/100‑5 (MQ=255)
           gtgATGCAGATCGCTTTTTCCCTTAAGGCATGTTAGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGGaaaaaatgaa                                                                                      >  2:4148883/1‑94 (MQ=255)
             gATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAGGATGGGAGGTAAAAAATGAAACg                                                                                   >  1:1442280/1‑100 (MQ=255)
              aTGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCACTCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAAGTAAAAAATGAAACgg                                                                                  <  1:2704332/100‑1 (MQ=255)
               tGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGt                                                                                 >  2:2848710/1‑100 (MQ=255)
                  aGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTggg                                                                              >  2:1519683/1‑100 (MQ=255)
                   gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                             >  1:2388873/1‑100 (MQ=255)
                   gATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGa                                                                             <  2:592250/100‑1 (MQ=255)
                       gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCACCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  2:2228233/1‑100 (MQ=255)
                       gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAATAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  1:1647367/1‑100 (MQ=255)
                       gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  1:2647826/1‑100 (MQ=255)
                       gCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTg                                                                         >  1:3716214/1‑100 (MQ=255)
                             tCCCTTCAAGCATTCTTGTTTAAGTCAGTCAAAGAAAGGCAATTCTCAACAACAAAATAAAGATGAGAAGTAAAAAAGGAAACTGTGGGAAGTGGCttt                                                                    <  2:1099864/99‑1 (MQ=255)
                              cccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTAc                                                                  <  1:2283137/100‑1 (MQ=255)
                               ccTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                 <  2:3070227/100‑1 (MQ=255)
                               ccTTCAGACATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACt                                                                 <  1:1677116/100‑1 (MQ=255)
                                cTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTg                                                                >  1:1028556/1‑100 (MQ=255)
                                 ttCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTgg                                                               <  2:9515/100‑1 (MQ=255)
                                  tCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGt                                                              <  1:634127/100‑1 (MQ=255)
                                    ggcattttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGAAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGa                                                            >  2:802427/6‑100 (MQ=255)
                                     ggCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGAt                                                           >  1:478782/1‑100 (MQ=255)
                                      gCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATg                                                          <  1:633634/100‑1 (MQ=255)
                                       cATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATgg                                                         <  2:1780109/100‑1 (MQ=255)
                                        aTTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGt                                                        <  2:301939/100‑1 (MQ=255)
                                         tttttGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTa                                                       >  1:3471238/1‑100 (MQ=255)
                                             tGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGt                                                   >  2:500821/1‑100 (MQ=255)
                                               tttAATTCAATTAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACTGTGGGAAGTGGCTTTACTGGTGATGGTAATGttn                                                 <  2:1762753/100‑2 (MQ=255)
                                               tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg                                                 <  1:2345014/100‑1 (MQ=255)
                                               tttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg                                                 <  1:472228/100‑1 (MQ=255)
                                                ttATTTCGATCAACGGAAGGCAGTTCTCAAAAACAAAATAAAGATGGGAGAGAAAAAATGAAACTGAGGGGAGTGAGTTTAAGGGAGATGGAAATggt                                                  >  2:3815457/1‑96 (MQ=255)
                                                ttAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                                <  1:4001554/100‑1 (MQ=255)
                                                cttccgatctcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTgg                                                <  1:2615949/91‑1 (MQ=255)
                                                 tAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGt                                               <  1:3028329/100‑1 (MQ=255)
                                                  aaTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGtt                                              >  2:2721990/1‑100 (MQ=255)
                                                   aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGAAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGtt                                              >  2:1443597/1‑99 (MQ=255)
                                                   aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                             >  1:3658891/1‑100 (MQ=255)
                                                   aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                             >  2:3412146/1‑100 (MQ=255)
                                                   aTTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGAAGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGttt                                             >  2:3393697/1‑100 (MQ=255)
                                                    ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg                                            <  1:4099108/100‑1 (MQ=255)
                                                    ttCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTg                                            >  1:2905777/1‑100 (MQ=255)
                                                     tcaatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGc                                           <  1:1990661/100‑1 (MQ=255)
                                                      caatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCt                                          >  1:310056/1‑100 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         <  2:3318578/100‑1 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         <  2:2772811/100‑1 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         <  1:2054760/100‑1 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         >  2:1719144/1‑100 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         <  2:1197454/100‑1 (MQ=255)
                                                       aatcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTg                                         >  1:4060974/1‑100 (MQ=255)
                                                         tcaaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATCAAACGGTGGGAAGTGGCTTTAGTGGTGATGGTAATGTTGGTTTGCTGt                                        >  2:522713/1‑99 (MQ=255)
                                                          caaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAt                                      >  2:2754975/1‑100 (MQ=255)
                                                           aaCGAAAGGCAGTTCCCAACAACAAACTACAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                     <  2:760718/100‑1 (MQ=255)
                                                           aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTAtt                                     <  2:2024052/100‑1 (MQ=255)
                                                           aaCGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTAATGGTGATGGTAATGTTGGTTTGCTGTAtt                                     >  1:972738/1‑100 (MQ=255)
                                                             cGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGa                                   >  2:1942084/1‑100 (MQ=255)
                                                               aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                 <  1:1674243/100‑1 (MQ=255)
                                                               aaaGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAAc                                 >  1:2628796/1‑100 (MQ=255)
                                                                 aGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtg                               <  1:3287984/100‑1 (MQ=255)
                                                                  ggCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgt                              <  2:2322397/100‑1 (MQ=255)
                                                                   gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             >  1:1017925/1‑100 (MQ=255)
                                                                   gCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             >  1:219260/1‑100 (MQ=255)
                                                                   gCAGTGCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             <  1:1591065/100‑1 (MQ=255)
                                                                     aGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACtgtg                             <  2:260250/98‑1 (MQ=255)
                                                                       ttCCCAACAACAAGATAAAGATGGGAGGTAAAAAAGTAAACGGTGGGAAGTGGCTTTACTGGTGATGGGAATGTTGGTTTGCTGTATTGAACTGTGAagg                         <  2:3474031/100‑1 (MQ=255)
                                                                       ttCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg                         <  1:2918101/100‑1 (MQ=255)
                                                                       ttCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagg                         <  1:515498/100‑1 (MQ=255)
                                                                        tCCCAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAagga                        >  2:2355807/1‑100 (MQ=255)
                                                                         cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                       >  1:2133424/1‑100 (MQ=255)
                                                                         cccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggag                       <  2:3117438/100‑1 (MQ=255)
                                                                          ccAACAACAAAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAaggagg                      <  2:2135176/100‑1 (MQ=255)
                                                                           caacaacaaAATAAAGATGTGAGGTAAAAAATGAAACTGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGaa                           <  2:4048239/94‑1 (MQ=255)
                                                                           caacaacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAggagga                     >  1:3413770/1‑100 (MQ=255)
                                                                              caacaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGc                  >  1:1630219/1‑100 (MQ=255)
                                                                                acaaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCa                <  1:1443606/100‑1 (MQ=255)
                                                                                 naaaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt               >  2:2348725/2‑100 (MQ=255)
                                                                                 caaAATAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCAt               <  1:2444875/100‑1 (MQ=255)
                                                                                  aaaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg              >  2:3275075/1‑100 (MQ=255)
                                                                                  aaaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATg              <  1:2966091/100‑1 (MQ=255)
                                                                                   aaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa             >  2:3397273/1‑100 (MQ=255)
                                                                                   aaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa             >  2:2467385/1‑100 (MQ=255)
                                                                                   aaaTAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGACTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGa             >  2:1681581/1‑100 (MQ=255)
                                                                                      tAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc          >  2:502392/1‑100 (MQ=255)
                                                                                      tAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc          <  1:4080215/100‑1 (MQ=255)
                                                                                      tAAAGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAAc          <  1:3350663/100‑1 (MQ=255)
                                                                                      tAAAGATGGAAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACACCATGAAAc          <  2:2287343/100‑1 (MQ=255)
                                                                                       aaaGATGTGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt         <  2:2585351/100‑1 (MQ=255)
                                                                                       aaaGATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACt         <  2:3693477/100‑1 (MQ=255)
                                                                                          gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt      >  2:3183970/1‑100 (MQ=255)
                                                                                          gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACtttt      <  1:3766905/100‑1 (MQ=255)
                                                                                          gATGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACGGTGAAGGAGGACGCCATGAAACtttt      >  2:877255/1‑100 (MQ=255)
                                                                                           aTGGGAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGACGGTAAAGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTa     >  2:925086/1‑100 (MQ=255)
                                                                                             gggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt   <  2:2788690/100‑1 (MQ=255)
                                                                                             gggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAAt   <  2:861118/100‑1 (MQ=255)
                                                                                              ggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc  >  2:236735/1‑100 (MQ=255)
                                                                                              ggAGGTAAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATc  <  1:2985972/100‑1 (MQ=255)
                                                                                                      |                                                                                              
AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTTGCTGTATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATC  >  NC_000913/2563414‑2563606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: