Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 497,139:1 (T)5→6 100% intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913497,1341.T95.7% 384.1 / 8.7 117intergenic (+140/‑41)htpG/adkprotein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base T (51/60);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

TTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497036‑497228
                                                                                                   |                                                                                                  
ttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTTACCGTTTCAGCCCCAGGTGCCTTTCTTGGGGCAATCGCCTGTTGGTGGTATCGTTTATCGCt                                                                                                   <  2:390035/100‑1 (MQ=255)
ttcATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCt                                                                                                   >  2:980437/1‑100 (MQ=255)
  catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                               >  1:2951149/1‑100 (MQ=255)
  catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttt                                                                                               >  1:2900091/1‑100 (MQ=255)
  catcatCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCACCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGCTATCGTTTATCGCtt                                                                                                 >  2:3967721/1‑99 (MQ=255)
   atcatcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttt                                                                                              >  2:2380561/1‑100 (MQ=255)
     catcTGCACTTTCCGCAAATTATCTGGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttttt                                                                                           >  2:1855320/1‑99 (MQ=255)
     catcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCtttttt                                                                                           >  1:1197681/1‑99 (MQ=255)
      atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTCGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                          <  1:2976752/100‑3 (MQ=255)
      atcTGCACTTTCCGCAAATTATCTCGCCATTAACCGTGTCAGCCCCATGTGCCTTGCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttc                                                                                          <  1:2037770/100‑3 (MQ=255)
        cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCTAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaa                                                                                        <  2:2659641/100‑5 (MQ=255)
        cTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGGCTGTTGGTGGTATCGTTTATCGCttttttca                                                                                         >  2:3475151/1‑96 (MQ=255)
         tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                       <  2:2416102/100‑6 (MQ=255)
         tGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCttttttcaaa                                                                                       <  1:1536133/100‑6 (MQ=255)
          gCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTGGGTGGTATCGTTTATCGCTTTTTTcaaaa                                                                                     <  1:678536/100‑1 (MQ=255)
            aCTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCaaaaaa                                                                                    >  2:2170986/1‑100 (MQ=255)
            aCTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCaaaaaa                                                                                    >  1:768738/1‑100 (MQ=255)
             cTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGCGCCTTTCTTGCGGCACTCGCCTGTTGGTGGTATCGTTTATCGCttttttcccaa                                                                                      >  2:1736740/1‑91 (MQ=255)
                tCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCg                                                                                <  1:3536916/100‑1 (MQ=255)
                 ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               <  1:407744/100‑1 (MQ=255)
                 ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               <  1:1972412/100‑1 (MQ=255)
                 ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               <  1:1565797/100‑1 (MQ=255)
                 ccGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               <  2:2446376/100‑1 (MQ=255)
                  cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  1:652626/1‑100 (MQ=255)
                  cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              >  1:1782684/1‑100 (MQ=255)
                  cGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGac                                                                              <  1:2781869/100‑1 (MQ=255)
                  cGCAAATTATCTCGCCATTAACCGTTTAAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGCTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGa                                                                               >  2:1857281/1‑99 (MQ=255)
                  cGCAAATTATATCGCCATTAACCGTTTCATCCCCGTCTTCCTTTCTTGTGGCAATCGCCTGTTGGTTGTATCGTTTACCGCTTTTTTCAAAAAATTCGac                                                                              <  2:984501/100‑1 (MQ=255)
                           tccccccATTACCCTTTCCAGCCCCAGTTCCCTTTTTGAAGCCATTCCCCTTTTGTTGGTATCTTTAATCGCTTTTTTCAAAAAATTCGACACAtttt                                                                       <  1:2464616/93‑1 (MQ=255)
                            ctcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTaa                                                                     >  2:1958317/1‑99 (MQ=255)
                             tcGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAgg                                                                   >  2:467624/1‑100 (MQ=255)
                              cGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGCGGAAATCGCCTGTTTGTGGTATCGTTTATCGCTTTTTCCAAAAAATTCGACACAGTTCAAggg                                                                  <  2:3397751/100‑1 (MQ=255)
                                ccATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAATTTATTCGACACATTTTAAGGGGa                                                                >  2:1278348/1‑100 (MQ=255)
                                ccATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGa                                                                <  1:3162260/100‑1 (MQ=255)
                                 cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAt                                                               >  1:1946907/1‑100 (MQ=255)
                                 cATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAt                                                               <  1:131304/100‑1 (MQ=255)
                                  aTTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCTACACATTTTAAgggtagt                                                              >  1:3750062/1‑96 (MQ=255)
                                   ttAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAttt                                                             >  2:569283/1‑100 (MQ=255)
                                   ttAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAttt                                                             >  1:2026097/1‑100 (MQ=255)
                                    tAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtttt                                                            >  1:2286518/1‑100 (MQ=255)
                                    tAACCGTTTCAGCCCCAGGTCCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATAGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGAtttt                                                            >  1:3926841/1‑100 (MQ=255)
                                      aCCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTc                                                           >  2:1363626/1‑99 (MQ=255)
                                       ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  2:2378128/100‑1 (MQ=255)
                                       ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         >  1:3799279/1‑100 (MQ=255)
                                       ccGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  2:1941388/100‑1 (MQ=255)
                                        cGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCa                                                        >  2:1246306/1‑100 (MQ=255)
                                        cGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATAGATAATAAAAAAAAATTCGACACATTTTAAGGGGATTTTCGc                                                         <  2:2775785/99‑1 (MQ=255)
                                             cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  2:1946897/100‑1 (MQ=255)
                                             cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  1:1705353/100‑1 (MQ=255)
                                             cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   >  2:1669927/1‑100 (MQ=255)
                                             cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   >  1:1148301/1‑100 (MQ=255)
                                             cAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCg                                                   >  1:297519/1‑100 (MQ=255)
                                              aGCTCCAGGTGCCTTTCTTGAGGAAATAGCTTGTTTGTGGTATCGTTTATCGCTTTTTTCAAAAAATTTGACACATTTTAAGGGGATTTTCGCAATGCg                                                   <  2:670139/99‑1 (MQ=255)
                                              aGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGt                                                  <  1:1437889/100‑1 (MQ=255)
                                              aGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGt                                                  <  1:730437/100‑1 (MQ=255)
                                               gCCCCAGGTGCCTTTCTTGAGTCAATCGCCTGTTGGTGGTATCCTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTa                                                 >  2:1838463/1‑100 (MQ=255)
                                                ccccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAt                                                <  2:1344513/100‑1 (MQ=255)
                                                ccccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTAt                                                >  1:580134/1‑100 (MQ=255)
                                                 cccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATc                                               <  1:140603/100‑1 (MQ=255)
                                                  ccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCa                                              <  2:545811/100‑1 (MQ=255)
                                                  ccAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGCGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCa                                              >  2:518622/1‑100 (MQ=255)
                                                   cAGGTGCCTTTCTTGATGCAATCGCCTGTTGGAGGTATCGTTTATCGCTTTTGTCAAAAAATTCAACACATGTTGAGGCTCTATTCGCAATCCGTATc                                               >  2:3455075/1‑98 (MQ=255)
                                                     ggTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTc                                           >  2:3887844/1‑100 (MQ=255)
                                                       tGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTg                                         <  1:3777835/100‑1 (MQ=255)
                                                       tGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTg                                         >  2:267722/1‑100 (MQ=255)
                                                        gCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGc                                        >  2:3122194/1‑100 (MQ=255)
                                                          cTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      >  2:3608087/1‑100 (MQ=255)
                                                          cTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCtt                                      <  2:3069485/100‑1 (MQ=255)
                                                           tttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTg                                     <  1:1408586/100‑1 (MQ=255)
                                                            ttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTgg                                    <  1:2076857/100‑1 (MQ=255)
                                                            ttCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTgg                                    <  1:1513235/100‑1 (MQ=255)
                                                             tCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgc                                   <  1:524586/100‑1 (MQ=255)
                                                              cTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcg                                  <  1:3211447/100‑1 (MQ=255)
                                                               ttGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGgcgc                                 >  2:1603609/1‑100 (MQ=255)
                                                                 gAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCTTTTATTTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTc                               >  2:1145761/1‑100 (MQ=255)
                                                                  aGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTc                               >  2:1537588/1‑99 (MQ=255)
                                                                    gCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCgg                            <  1:3874586/100‑1 (MQ=255)
                                                                    gCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTCGCGCTCCg                             <  2:2912599/99‑1 (MQ=255)
                                                                       aTCGCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGgcg                         <  1:994309/100‑1 (MQ=255)
                                                                       aTCGCCTGTTGGTGGGATCGTTTATCGCTTATTACAAAAAGTTCGACACATTTTAAGGGGGTTTTCGCAAGTCGTATGATTATGCTTGACGCTTCTGgca                         >  2:3640655/1‑99 (MQ=255)
                                                                          gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAGTTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      >  2:2234385/1‑100 (MQ=255)
                                                                          gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:267725/100‑1 (MQ=255)
                                                                          gCCTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgg                      <  1:500400/100‑1 (MQ=255)
                                                                            cTGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCgggg                    <  1:2368633/100‑1 (MQ=255)
                                                                             tGTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGa                   >  2:1555746/1‑100 (MQ=255)
                                                                              gTTGGTGGTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGaa                  >  1:931410/1‑100 (MQ=255)
                                                                                tggtggTATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAg                <  1:1555755/100‑1 (MQ=255)
                                                                                  gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAGGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:1517168/100‑1 (MQ=255)
                                                                                  gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              <  1:654484/100‑1 (MQ=255)
                                                                                  gtggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAggg              >  2:2619073/1‑100 (MQ=255)
                                                                                    ggtATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            >  1:1894400/1‑100 (MQ=255)
                                                                                    ggtATCGTTTATCGCTTTTTTAAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGAc            <  2:1256477/100‑1 (MQ=255)
                                                                                      tATCGTTTATCGCTTTTTTCAAAAAATTCGACACCTTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGTCGCGGGGCAAGGGAc            >  2:463509/1‑98 (MQ=255)
                                                                                      tATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTc          >  1:1169414/1‑100 (MQ=255)
                                                                                      tATCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTc          >  1:2972207/1‑100 (MQ=255)
                                                                                        tCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAg        <  1:2660622/100‑1 (MQ=255)
                                                                                        tCGTTTATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAg        <  1:3617166/100‑1 (MQ=255)
                                                                                           tttATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCt     <  1:739782/100‑1 (MQ=255)
                                                                                            ttATCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTc    <  1:3450652/100‑1 (MQ=255)
                                                                                              aTCGCTTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAg  <  2:2158441/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGCGTATCATTCTGCTTGGCGCTCCGGGCGCGGGGAAAGGGACTCAGGCTCAG  >  NC_000913/497036‑497228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: