Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,349,085:1 +C 100% intergenic (‑341/+230) dcuB ← / ← dcuR C4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,349,0821.C69.2% 506.4 / 276.0 224intergenic (‑338/+233)dcuB/dcuRC4‑dicarboxylate transporter, anaerobic; DcuS co‑sensor/response regulator in two‑component regulatory system with DcuS
Reads supporting (aligned to +/- strand):  ref base . (39/30);  new base C (81/72);  total (121/103)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 235 total aligned reads displayed.

GATGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGA  >  NC_000913/4348984‑4349180
                                                                                                   |                                                                                                  
  tGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTAccc                                                                                                 <  1:2590679/100‑1 (MQ=255)
  tGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTAccc                                                                                                 >  1:635940/1‑100 (MQ=255)
    ttAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTACTCTGATCTAcccca                                                                                               <  1:1386539/100‑3 (MQ=255)
    ttAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTAcccca                                                                                               <  2:1220600/100‑3 (MQ=255)
      aaTGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTTTTTTTTTTTTATATTTCATTAGGTTTATTGATTAATTTGATCTActgtatt                                                                                             >  2:2571176/1‑94 (MQ=255)
       aTGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTAccccattt                                                                                            >  1:2085833/1‑95 (MQ=255)
        tgttttgtttGCTGTTATCTTATTGATATTTATCTGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTtg                                                                                            <  2:2479572/100‑1 (MQ=255)
        tgttttgtttGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTtg                                                                                            >  1:31413/1‑100 (MQ=255)
          ttttgtttGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTtgtg                                                                                          <  1:690164/100‑1 (MQ=255)
           tttgtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATCGATTAAGCGGATCTACCCATTTGTggg                                                                                        <  2:186510/100‑1 (MQ=255)
           tttgtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTgg                                                                                         >  1:109065/1‑100 (MQ=255)
           tttgtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTgg                                                                                         <  1:1660161/100‑1 (MQ=255)
            ttgtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTggg                                                                                        <  1:3108348/100‑1 (MQ=255)
            ttgtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTggg                                                                                        <  1:1226047/100‑1 (MQ=255)
              gtttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTaa                                                                                     >  1:1238016/1‑100 (MQ=255)
               tttgCTGTTATCTTATTGATATTTATCGGTCGATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAAGCTGATCTACCCCATTTGTGGGt                                                                                       <  2:1150097/98‑1 (MQ=255)
                ttgCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTaaa                                                                                    <  1:491359/100‑1 (MQ=255)
                   cTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAAt                                                                                 >  1:1556789/1‑100 (MQ=255)
                   cTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAAt                                                                                 >  2:3292244/1‑100 (MQ=255)
                    tGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATac                                                                               <  2:2279047/100‑1 (MQ=255)
                      ttATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATaca                                                                              <  1:1498355/100‑1 (MQ=255)
                        aTCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATAcaca                                                                            <  1:1864790/100‑1 (MQ=255)
                         tCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACAt                                                                           >  1:3073144/1‑100 (MQ=255)
                              ttGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGc                                                                      <  2:190187/100‑1 (MQ=255)
                                gATATTTATCGGTCTATTTTTTTTTTTTTTTTCAGATGTAATAAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGc                                                                      >  2:1553312/1‑98 (MQ=255)
                                 atatTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCggg                                                                   <  2:4101427/100‑1 (MQ=255)
                                 atatTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCggg                                                                   >  1:3708931/1‑100 (MQ=255)
                                 atatTTAGCGATCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATAcacagatcggtag                                                                   >  2:1800979/1‑90 (MQ=255)
                                  tatTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGt                                                                  >  1:1827471/1‑100 (MQ=255)
                                  tatTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTg                                                                 <  1:1817374/100‑1 (MQ=255)
                                   atTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTg                                                                 >  1:2978729/1‑100 (MQ=255)
                                   atTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGa                                                                <  1:1361766/100‑1 (MQ=255)
                                     ttATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                                                               <  2:3073126/100‑1 (MQ=255)
                                     ttATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGAc                                                               <  1:2935185/100‑1 (MQ=255)
                                      tATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACat                                                             <  1:951201/100‑1 (MQ=255)
                                          ggTCTATTTTTTTTTTTTTTTTCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTATAAATACACATAATGCGGGTGACattat                                                          >  2:2018645/1‑100 (MQ=255)
                                          ggTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACataat                                                          <  1:3443641/100‑1 (MQ=255)
                                            ttttttttttGTTCTCAGGTCAGATGTCATTATGTTTATAGATTAATCTTATCTATCTCATTTTTGTGTAAAAATACACATAATGCTTGTGACATAATAg                                                        <  2:1352530/96‑1 (MQ=255)
                                              tATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGtt                                                      <  1:2439832/100‑1 (MQ=255)
                                               aTTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGtta                                                     <  2:706396/100‑1 (MQ=255)
                                               aTTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaa                                                    >  2:53291/1‑100 (MQ=255)
                                                ttttttGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaa                                                    >  1:3539400/1‑100 (MQ=255)
                                                ttttttGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaat                                                   <  1:925916/100‑1 (MQ=255)
                                                   tttGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGttaatta                                                 <  2:3548866/100‑1 (MQ=255)
                                                      gTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACt                                              <  1:3852552/100‑1 (MQ=255)
                                                       ttCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACttt                                            >  2:763217/1‑100 (MQ=255)
                                                        tctcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACttt                                            >  2:491541/1‑100 (MQ=255)
                                                        tctcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACttt                                            >  1:4056816/1‑100 (MQ=255)
                                                         ctcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTg                                          >  2:2942815/1‑100 (MQ=255)
                                                          tcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACgtt                                            >  1:2491282/1‑98 (MQ=255)
                                                          tcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTg                                          <  2:283565/100‑1 (MQ=255)
                                                          tcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTg                                          >  2:1540653/1‑100 (MQ=255)
                                                           cAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGt                                         >  2:1855459/1‑100 (MQ=255)
                                                           cAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGt                                         >  2:3489770/1‑100 (MQ=255)
                                                            aGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGtt                                        >  1:2694821/1‑100 (MQ=255)
                                                            aGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGtt                                        <  1:1410204/100‑1 (MQ=255)
                                                             gggCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTa                                       >  2:3822671/1‑100 (MQ=255)
                                                             gggCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTa                                       >  1:732665/1‑100 (MQ=255)
                                                              ggCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAg                                      <  2:3371172/100‑1 (MQ=255)
                                                                  gATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGttt                                 >  1:3670867/1‑100 (MQ=255)
                                                                  gATGTCAGTAGGTTTATAGATTAATGTGAGCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGttt                                 <  2:1514838/100‑1 (MQ=255)
                                                                   aTGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGttt                                 >  2:3397152/1‑100 (MQ=255)
                                                                   aTGTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGAGGGCGACATAATAGTTAATTAACTTTTGTTAGCGtt                                  >  2:3344594/1‑99 (MQ=255)
                                                                     gTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTg                               <  2:4148568/100‑1 (MQ=255)
                                                                     gTCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTg                               <  1:2645765/100‑1 (MQ=255)
                                                                      tCATTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGa                              <  2:4090758/100‑1 (MQ=255)
                                                                       cATTAGGTTTATAGATTAATCTGATCTACCCCCTTTGTGGGTAAAAATAAACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGaa                             >  2:1037592/1‑100 (MQ=255)
                                                                        aTTAGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGaaa                            >  2:1788404/1‑100 (MQ=255)
                                                                          tAGGTTTATAGATTCATCTGCTATCACTCTTTGTGGGTAAACATATTCATAATGTGGGTGAGATAATAGTTAATTATCTTTTGTTAGCGTTTTGAAATTa                         <  2:344681/100‑1 (MQ=255)
                                                                           aGGTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTa                         <  1:2298512/100‑1 (MQ=255)
                                                                            ggTTTATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATTCGGGTGACATAATAGTTAATTAACATTTGTTAGCGTTTTGAAATTaa                        >  2:1243199/1‑100 (MQ=255)
                                                                            ggTTTATAGATTAATCTGATCTACCCATTTTTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGACATTaaa                       >  2:157549/1‑100 (MQ=255)
                                                                              tttATAGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTaaaa                      <  1:2313335/100‑1 (MQ=255)
                                                                                tataGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATCATACGGGTGACATAATAGTTAATTAACAATTGTTAGCGTTTTGAAATTAAAAac                    >  2:2633732/1‑100 (MQ=255)
                                                                                 ataGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAaca                   >  2:1744986/1‑100 (MQ=255)
                                                                                  taGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAacac                  >  1:2757747/1‑100 (MQ=255)
                                                                                   ngATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAacac                  >  2:3984229/2‑99 (MQ=255)
                                                                                   aGATTAATCTGATCTACCCCATTTGTGTGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAacac                  <  2:3748194/99‑1 (MQ=255)
                                                                                   aGATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACAcc                 <  1:471415/100‑1 (MQ=255)
                                                                                    gATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCg                >  1:589307/1‑100 (MQ=255)
                                                                                    gATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCg                <  1:3984259/100‑1 (MQ=255)
                                                                                    gATTAATCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAAGTAACTTTTATTAGCGTTTTGAAATTAAAAacac                  >  2:3683294/1‑98 (MQ=255)
                                                                                    gATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAacac                  >  2:3691806/1‑97 (MQ=255)
                                                                                    gATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGt               >  1:2006704/1‑100 (MQ=255)
                                                                                       tAATCTGATCTACGCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGttn             <  2:2838659/99‑2 (MQ=255)
                                                                                        aaTCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCa            >  2:4009891/1‑100 (MQ=255)
                                                                                        aaTCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAc           >  1:2367401/1‑100 (MQ=255)
                                                                                         aTCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACGGTTCAc           >  2:1394199/1‑100 (MQ=255)
                                                                                         aTCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAc           >  1:3749152/1‑100 (MQ=255)
                                                                                         aTCTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAc           >  1:3398204/1‑100 (MQ=255)
                                                                                         aTCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAcc          >  1:2101662/1‑100 (MQ=255)
                                                                                         aTCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAc           >  2:2776381/1‑99 (MQ=255)
                                                                                         aTCTGATATACCCCATTTGTGAGTAAAAATACACATAAGGCGGTTAACATAATAGTTAATTAACTTATGTTAGCGTTTTGAAAGTtaaaagagcact              >  2:3530390/1‑90 (MQ=255)
                                                                                          tCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCt         <  2:635936/100‑1 (MQ=255)
                                                                                           cTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCAcc          >  2:3140085/1‑99 (MQ=255)
                                                                                           cTGATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCt         >  2:208303/1‑100 (MQ=255)
                                                                                           cTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTg        >  2:2372764/1‑100 (MQ=255)
                                                                                            tGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGa       <  1:53292/100‑1 (MQ=255)
                                                                                             gATCTACCCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGa       <  2:2165998/100‑1 (MQ=255)
                                                                                                cTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAagag   <  2:2889328/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GATGTTAATGTTTTGTTTGCTGTTATCTTATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGA  >  NC_000913/4348984‑4349180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: