Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,561:1 +C 15.8% coding (1120/1452 nt) uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,5611.C15.8% 432.1 / 90.1 133coding (1120/1452 nt)uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base . (60/52);  new base C (12/9);  total (72/61)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

Only 100 of 145 total aligned reads displayed.

CGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACT  >  NC_000913/1609462‑1609660
                                                                                                    |                                                                                                   
cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                      >  2:2865945/1‑100 (MQ=255)
cGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                      >  1:3327993/1‑100 (MQ=255)
 gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGGCGTTTGCCTTCTGCCCTGCCAGCAGCtg                                                                                                      <  2:3543555/99‑1 (MQ=255)
 gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgt                                                                                                    >  1:1280462/1‑100 (MQ=255)
  ctgTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                                   <  2:4053334/98‑1 (MQ=255)
  ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                                   <  1:1566092/100‑1 (MQ=255)
   cGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTgg                                                                                                  >  1:3942808/1‑100 (MQ=255)
    gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtgag                                                                                                 >  1:4053365/1‑98 (MQ=255)
    gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                 <  2:3346396/100‑1 (MQ=255)
     ttACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTTGCa                                                                                                <  1:1863895/100‑1 (MQ=255)
      tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                               <  2:915655/100‑1 (MQ=255)
      tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAg                                                                                               <  1:2321455/100‑1 (MQ=255)
        cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                             <  1:69420/100‑2 (MQ=255)
        cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAggg                                                                                             <  1:2276107/100‑2 (MQ=255)
        cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGCGTGCCGTTTGCCTTCTGCCCTCCCACAGCtgctcgcagg                                                                                              <  2:3506411/99‑9 (MQ=255)
         gCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggc                                                                                            <  1:802229/100‑3 (MQ=255)
         gCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggc                                                                                            <  1:2491023/100‑3 (MQ=255)
          cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                           <  1:3453607/100‑4 (MQ=255)
          cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                           <  1:2855142/100‑4 (MQ=255)
             ttccgatctgAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCgg                                                                                        <  1:3702741/91‑1 (MQ=255)
              ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                       <  1:3245963/100‑1 (MQ=255)
              ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                       <  2:1426450/100‑1 (MQ=255)
              ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                       >  1:3127896/1‑100 (MQ=255)
              ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                       <  2:603795/100‑1 (MQ=255)
                acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGt                                                                                      >  1:3566280/1‑99 (MQ=255)
                acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                                     <  1:50356/100‑1 (MQ=255)
                acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTg                                                                                     <  1:2429697/100‑1 (MQ=255)
                 cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGc                                                                                    <  2:1182581/100‑1 (MQ=255)
                  gATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGGGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCg                                                                                   <  2:4031435/100‑1 (MQ=255)
                   aTAGAACGCAATCAATGCCGATAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGcn                                                                                   <  2:2877673/99‑2 (MQ=255)
                     aGAACGCAATCAATGACGCTCATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCGTGCCAGCAGCTGTGGCTGGATGCGGGTGCg                                                                                   >  2:3451363/1‑97 (MQ=255)
                      gAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggagatcggaa                                                                               >  2:3702714/1‑91 (MQ=255)
                        aCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                             >  2:2123730/1‑100 (MQ=255)
                        aCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                             >  1:307689/1‑100 (MQ=255)
                          gCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                           <  1:3844774/100‑1 (MQ=255)
                           caatcaatGTCGCTCATGCGACAGTAAGGCGCGCAGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGTTTGCGGGTGCTGAACTTgtt                                                                          <  2:3477183/100‑3 (MQ=255)
                           caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                          >  2:3126373/1‑100 (MQ=255)
                                aatGTCGCTAATGCGAAAGTAAGGCGCGTCGGAAGTTTGGCGTTGGCCTTCTGCCCTGCCATCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCa                                                                        <  2:2917218/97‑1 (MQ=255)
                                  tGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCg                                                                   >  1:2710760/1‑100 (MQ=255)
                                    ccGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGCACTTGGTCATACCGtt                                                                 >  2:181652/1‑100 (MQ=255)
                                      gCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGa                                                               >  2:635810/1‑100 (MQ=255)
                                          aTGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgcgc                                                           >  1:250273/1‑100 (MQ=255)
                                           tgggAAAGTGAGGCGCGCCGGAAGTGTGCCGTTTGCCGTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                          <  2:1060460/97‑1 (MQ=255)
                                            gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa                                                         >  2:1904000/1‑100 (MQ=255)
                                             cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcggt                                                        >  2:392428/1‑98 (MQ=255)
                                             cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                        <  1:1880439/100‑1 (MQ=255)
                                             cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGAt                                                        >  2:3006580/1‑100 (MQ=255)
                                              gAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATa                                                       <  2:1273105/100‑1 (MQ=255)
                                                aaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGa                                                     >  1:562207/1‑100 (MQ=255)
                                                   tAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcag                                                  >  1:3660243/1‑100 (MQ=255)
                                                     aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                >  1:2347088/1‑100 (MQ=255)
                                                     aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                <  2:754115/100‑1 (MQ=255)
                                                     aGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                >  1:1362314/1‑100 (MQ=255)
                                                      ggCGCGCGGGAAGTGTGCCGTTTGCCATCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                               >  1:2627184/1‑100 (MQ=255)
                                                       gcgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACagcagc                                              >  1:2730389/1‑100 (MQ=255)
                                                           gcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGAt                                          <  1:601270/100‑1 (MQ=255)
                                                            ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                         <  2:1939007/100‑1 (MQ=255)
                                                            ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                         >  1:3924848/1‑100 (MQ=255)
                                                             cGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGc                                        <  1:1807547/100‑1 (MQ=255)
                                                             cGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGc                                        <  2:1604551/100‑1 (MQ=255)
                                                              ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt                                       >  1:2180401/1‑100 (MQ=255)
                                                                   tgtgCCGGTTGCGGTCGGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCGTACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATg                                  <  2:1311108/100‑1 (MQ=255)
                                                                    gtgCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGt                                 >  1:2488007/1‑100 (MQ=255)
                                                                       ccGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACg                              >  1:1707717/1‑100 (MQ=255)
                                                                         gTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                            <  1:2009738/100‑1 (MQ=255)
                                                                         gTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACggg                            <  1:925734/100‑1 (MQ=255)
                                                                             gCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTAc                        <  2:220684/100‑1 (MQ=255)
                                                                              ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTatg                       >  2:2520319/1‑98 (MQ=255)
                                                                              ccTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                       >  2:2744834/1‑100 (MQ=255)
                                                                               cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                      >  1:1295777/8‑100 (MQ=38)
                                                                               cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                      >  2:4172249/8‑100 (MQ=38)
                                                                               cTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACgg                      >  2:2903825/1‑100 (MQ=255)
                                                                                ttCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa                     <  1:751301/100‑1 (MQ=255)
                                                                                ttCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa                     <  1:967448/100‑1 (MQ=255)
                                                                                  gcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                   >  1:2071292/5‑100 (MQ=255)
                                                                                  gcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                   >  2:435135/5‑100 (MQ=255)
                                                                                  gcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                   >  1:2903189/5‑100 (MQ=255)
                                                                                  ctgccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTAGGGGTTACGGaaa                   <  1:2745622/100‑1 (MQ=255)
                                                                                   catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                  >  1:1538058/4‑100 (MQ=255)
                                                                                   catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                  >  2:2374614/4‑100 (MQ=255)
                                                                                   catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                  >  1:3518079/4‑100 (MQ=255)
                                                                                   catcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                  >  1:3884247/4‑100 (MQ=255)
                                                                                    gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                 <  1:2987888/100‑1 (MQ=255)
                                                                                    gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                 >  1:1652848/1‑100 (MQ=255)
                                                                                    gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                 >  2:1129441/1‑100 (MQ=255)
                                                                                    gccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCCTAATGTACGGGTTACGGAAACg                 <  2:2421176/100‑1 (MQ=255)
                                                                                    gccctaccAGCAGCTGTGGCAGGATGCGGGTGCGGAAGTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                 >  2:2223474/1‑100 (MQ=255)
                                                                                    atcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg                 >  1:1067241/3‑100 (MQ=255)
                                                                                      cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc               >  1:3678369/1‑100 (MQ=255)
                                                                                        tgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCg             <  2:3924818/100‑1 (MQ=255)
                                                                                         gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg            <  2:1759504/100‑1 (MQ=255)
                                                                                         gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg            >  1:3817789/1‑100 (MQ=255)
                                                                                         gccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCgg            <  1:1960382/100‑1 (MQ=255)
                                                                                           cagcagCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTg          <  2:2466646/100‑1 (MQ=255)
                                                                                             gcagcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc        >  1:2957535/1‑100 (MQ=255)
                                                                                               agcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTg      >  1:3099347/1‑100 (MQ=255)
                                                                                               agcTGTGGCAGGATGCGGGTGCGGAACGTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTg      <  2:1400842/100‑1 (MQ=255)
                                                                                                gcTGTGGCAGGATGCGGGTGCGGACCTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTCATGTACGGGTTACGGAAACGCCCGGTGACTGc     >  2:1215429/1‑100 (MQ=255)
                                                                                                  tgtgGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc   >  2:2912615/1‑100 (MQ=255)
                                                                                                   gtgGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACt  <  1:2014981/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
CGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACT  >  NC_000913/1609462‑1609660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: