New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4597603 =175 (1.160)10 (0.070) 8/162 NT 6.4% coding (1839/2292 nt) opgB OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
?NC_000913 4597636 = 131 (0.960)coding (1806/2292 nt) opgB OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
Rejected: Frequency below cutoff threshold.

TGGCGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4597708‑4597603
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgtcgatGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTC  >  NC_000913/4597636‑4597734
                                                                                                                                                                                                             
TGGCGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAAT                                                                                                           >  2:3250203/1‑100
 GGCGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAAgg                                                                                                          <  1:2807253/100‑3
  GCGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGT                                                                                                         >  2:328882/1‑100
   CGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTC                                                                                                        <  1:1256542/100‑1
   CGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTC                                                                                                        <  2:4160940/100‑1
   CGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTC                                                                                                        >  1:2136267/1‑100
   CGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTC                                                                                                        <  2:3126920/100‑1
      TCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGAT                                                                                                     >  2:1326249/1‑100
      TCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAAAGGCATTTAAAGAAACGGTCATCGACAGAGCACGTCACAAAGGCAATGCCGA                                                                                                      >  2:3287816/1‑99
                                                             AAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGA                                              >  2:1702952/1‑100
                                                                     GGTCATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTG                                      >  1:446296/1‑100
                                                                      GTCATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTcc                                     >  1:450094/1‑98
                                                                      GTCATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTACCATGTTGTTTTGTCAACATGCTGAACAATTTGC                                     >  1:2847355/1‑100
                                                                        CATCGACATGGCGCGTTACAAAGGCAATGTCGCTGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTG                                   >  2:3339290/1‑100
                                                                        CATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTac                                     >  2:2668016/1‑96
                                                                         ATCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGA                                  >  2:711087/1‑100
                                                                          TCGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGAC                                 >  1:1323003/1‑100
                                                                           CGACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACC                                >  2:1659008/1‑100
                                                                            GACATGGCGCGTTACAAAGGCAATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCG                               >  2:1284636/1‑100
                                                                                                  ATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACAC         >  2:2351315/1‑100
                                                                                                   TGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACA        >  2:2986938/1‑100
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      <  2:1454630/100‑1
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      <  2:4025884/100‑1
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      <  1:2691705/100‑1
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      <  1:3107843/100‑1
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      >  2:3286840/1‑100
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACC      <  2:2166883/100‑1
                                                                                                     TCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAACTGCCCTTGCGAGACACACC      <  2:2554877/100‑1
                                                                                                      CGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCA     >  2:1931798/1‑100
                                                                                                      CGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCA     >  1:1409934/1‑100
                                                                                                       GATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAG    <  1:3538083/100‑1
                                                                                                       GATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAG    <  1:3073025/100‑1
                                                                                                        ATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGT   <  1:3457182/100‑1
                                                                                                         TGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTC  <  2:1805304/100‑1
                                                                                                                                                                                                             
TGGCGGTCAGCAAATTGTTCAGCATGTTGACAAAACAACATGGCAGGGCAAAACGGCATTTAAAGATACGGTCATCGACATGGCGCGTTACAAAGGCAATGTCGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4597708‑4597603
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgtcgatGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAACATGCTGAACAATTTGCTGACCGCCAAGCTGCCCTTGCGAGACACACCAGTC  >  NC_000913/4597636‑4597734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.