New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4631855154 (1.020)10 (0.070) 3/166 NT 6.5% coding (1123/1938 nt) slt lytic murein transglycosylase, soluble
?NC_000913 = 4631875 142 (1.010)coding (1143/1938 nt) slt lytic murein transglycosylase, soluble
Rejected: Frequency below cutoff threshold.

TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4631751‑4631855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcaacCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAACG  <  NC_000913/4631875‑4631777
                                                                                                                                                                                                            
TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCAT                                                                                                          >  1:3009278/1‑100
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCCACTCATG                                                                                                         <  1:2180784/100‑1
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                         >  2:3767580/1‑100
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                         >  1:2163289/1‑100
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                         >  2:3485332/1‑100
 GGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                         <  1:583925/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                       <  2:953442/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                       <  2:1627640/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                       <  1:3485358/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                       <  2:643740/100‑1
   AAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCA                                                                                                       <  1:1957284/100‑1
    AGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAA                                                                                                      >  1:3377937/1‑100
     GCGAAAGAGACAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTTAACCAAAAGAGATTTTGCATCAACTCATGCAAC                                                                                                     <  2:2745377/100‑1
     GCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC                                                                                                     >  2:1334440/1‑100
      CGAAAGAGAAATATGAATGGCTTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTTAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATG                                                                                                         <  1:3484828/95‑1
      CGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC                                                                                                     >  2:2607567/1‑99
          AGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCG                                                                                                >  1:3636436/1‑100
                                TGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCAT                                                                          <  2:3636409/100‑1
                                TGGCAGGCGGATTCATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCAT                                                                          <  1:3004790/100‑1
                                TGGCAGGCGGATTCATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCAT                                                                          <  1:912424/100‑1
                                 GGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGTATG                                                                         <  2:4006836/100‑1
                                 GGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATG                                                                         <  2:546296/100‑1
                                 GGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATG                                                                         <  2:2704640/100‑1
                                  GCAGGCGGATTTATTGCTGGAACGTGTACGTTAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATG                                                                         <  2:2343743/99‑1
                                  GCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAATCATCGGGTAGAAACCACGCTGTTGCATG                                                                         <  1:802305/99‑1
                                    AGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGT                                                                      <  1:1521466/100‑1
                                    AGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGT                                                                      <  2:2285046/100‑1
                                                                                                   TGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAG       >  2:2282653/1‑100
                                                                                                   TGCAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAG       <  1:2768492/100‑1
                                                                                                   TGCAACCATCGGGTAGAAACCACGCTGTTGCATGACTTGATGCAAAATCCCTTTTGCTTCAGCTTAACGTCCGCGTTCCCGCAATAAATCCGCCTGCCAG       >  2:552287/1‑100
                                                                                                    GCAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGT      >  2:1452331/1‑100
                                                                                                    GCAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGT      <  2:454079/100‑1
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTA     >  2:3740928/1‑100
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTA     <  2:764611/100‑1
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTA     >  2:888988/1‑100
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTA     >  1:1689237/1‑100
                                                                                                     CAACCATCGGGTAGAAACCACGCTGTTGCATGAGTAGATGCAAAATCTCTTTTGCTTTAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTg     <  2:791403/100‑2
                                                                                                        CCATCGGGTAGAAACCGCGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAAGAAATCCGCATGCCAGTAAC   <  2:2600968/99‑1
                                                                                                        CCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAACG  <  2:2631716/100‑1
                                                                                                        CCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAACG  >  1:3930593/1‑100
                                                                                                        CCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAAC   >  1:801905/1‑99
                                                                                                        CCATCGGGTAGAAACCAAACTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTACGCGATCCAGCAATAAATCCGCCTGCCAGTAACG  >  2:175856/1‑100
                                                                                                                                                                                                            
TGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4631751‑4631855
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcaacCATCGGGTAGAAACCACGCTGTTGCATGAGTTGATGCAAAATCTCTTTTGCTTCAGCTTCACGTCCGCGTTCCAGCAATAAATCCGCCTGCCAGTAACG  <  NC_000913/4631875‑4631777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.