Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 36,231 T→C 100% intergenic (‑69/+40) caiD ← / ← caiC carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091336,2310TC98.6% 625.6 / ‑3.0 191intergenic (‑69/+40)caiD/caiCcarnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base C (79/105);  total (80/111)
Fisher's exact test for biased strand distribution p-value = 7.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TGCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT  >  NC_000913/36135‑36327
                                                                                                |                                                                                                
 gCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCTTGGACGAAAAAAAAAAAAAAAAAAATGCATATTGTTTTAGAGCGTGa                                                                                              <  2:1152966/100‑1 (MQ=255)
   gggTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGatt                                                                                            <  1:3112112/100‑1 (MQ=255)
        aGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAg                                                                                       <  1:2274687/100‑1 (MQ=255)
         gATGTAATGATTCACTCATTCCTTTTCTCCATTTTTTCTTTTTTTGTGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGc                                                                                      >  2:1269797/1‑100 (MQ=255)
          aTGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCt                                                                                     <  1:3502504/100‑1 (MQ=255)
          aTGCAATGATTCACTCATTCCATTTATCCATTTTTGCTTTTTTTGTGACGAAAAAAAAAAAAAAAAAAATGCATATTGTTTTAGAGCGTGAttatta                                                                                        <  2:699172/97‑1 (MQ=255)
           tGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTg                                                                                    <  2:591209/100‑1 (MQ=255)
            gTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTgg                                                                                   <  2:1942579/100‑1 (MQ=255)
            gTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTgg                                                                                   >  1:18460/1‑100 (MQ=255)
             tAATGCTTCACTCATTCCTTTTGTCAATTTTTGCTTTTTCTGGGACGACAAAATAAAAAATAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTg                                                                                    <  2:2232468/98‑1 (MQ=255)
             tAATGATTCACTCATTCTTTGTCTCCATTTTTGCTTTTTCAGGGTCGACAACATCCCTGCAAAAACTGCATATTGTTTTAGAGCGTGATTATTAGCTGGc                                                                                  <  2:3709735/100‑1 (MQ=255)
             tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTgg                                                                                   >  1:1412838/1‑99 (MQ=255)
             tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc                                                                                  >  1:691784/1‑100 (MQ=255)
             tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc                                                                                  <  2:1806103/100‑1 (MQ=255)
             tAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGc                                                                                  <  1:2043789/100‑1 (MQ=255)
              aaTGATTCACTCATTCCTTTTCTCCATTTTTTTATTTTTTTTTACGACAACATCCCTACAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTg                                                                                    >  2:2508223/1‑97 (MQ=255)
                tGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAgg                                                                               >  1:2069945/1‑100 (MQ=255)
                  aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCGAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt                                                                             <  1:1159918/100‑1 (MQ=255)
                  aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAgggn                                                                             <  1:1627443/100‑2 (MQ=255)
                  aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt                                                                             >  1:1458899/1‑100 (MQ=255)
                  aTTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGt                                                                             <  1:3739520/100‑1 (MQ=255)
                   ttCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCCTATTGTTTTAGAGCGTGATTGTTAGCTGGCAGGGTa                                                                            >  1:4112649/1‑100 (MQ=255)
                   ttCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTa                                                                            <  1:2766673/100‑1 (MQ=255)
                     cACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGt                                                                          >  1:798530/1‑100 (MQ=255)
                         cATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCATCCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTctc                                                                      >  2:358893/1‑98 (MQ=255)
                          aTTCCTTTTCTCCATTTTTTTTTTTTTTGTGACGACAACATCGCTGTAAATATTGTATATTGTTTTAGAGCGTGATTATTAGCTGTCTgggattgttgct                                                                     >  2:2136005/1‑91 (MQ=255)
                           ttCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCctg                                                                    >  1:121705/1‑100 (MQ=255)
                            tCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCctgc                                                                   <  2:347071/100‑1 (MQ=255)
                            tCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCctgc                                                                   >  1:3349055/1‑100 (MQ=255)
                              cTTTTCTCCATTTTTTTTTTTTTTTTTACGACAACATCCCTGCAAAAAATGTTTATTTTTTTAGAGCGTGATTATTAGCTTTCTGGGTAGTTcttttct                                                                  >  2:106045/1‑93 (MQ=255)
                              cTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCctgctg                                                                 <  2:3167718/100‑1 (MQ=255)
                               ttttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGAACTACCctgccag                                                                <  1:942432/100‑4 (MQ=255)
                               ttttCTCCATTTTTGCGTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGt                                                                <  1:1562320/100‑1 (MQ=255)
                                tttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGtt                                                               <  1:2628942/100‑1 (MQ=255)
                                tttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGtt                                                               <  1:1733880/100‑1 (MQ=255)
                                tttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGAACTACCctgccagc                                                               <  1:1376187/100‑5 (MQ=255)
                                tttCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTAGCAGGGAACTACCctgccagc                                                               <  1:2625782/100‑5 (MQ=255)
                                  tctcCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGAACTACCctgccagcta                                                             <  1:3905733/100‑7 (MQ=255)
                                    tcCATTTTTTCTTTTTCTGTGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTCATTATTAGCTCGCAGGGTAGTTCCCTTTTGTTTCat                                                           >  2:3219678/1‑100 (MQ=255)
                                    tcCATTCTTGCTGTTTCATGGACTACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGAACTACCctgccagctaat                                                           <  2:3089191/100‑9 (MQ=255)
                                     ccATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCatt                                                          >  1:3389367/1‑100 (MQ=255)
                                      cATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCattt                                                         <  1:1907127/100‑1 (MQ=255)
                                       aTTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCattta                                                        >  1:3621860/1‑100 (MQ=255)
                                        tttttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCattta                                                        <  1:2340621/99‑1 (MQ=255)
                                          tttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCatttattt                                                     <  1:85069/100‑1 (MQ=255)
                                          tttGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCatttattt                                                     >  1:3020746/1‑100 (MQ=255)
                                            ttcTTTTTCATGGACCACGACATCCCTGCAAGAAGTGCATATTGTTTTAGGGCGTGATTATTATCTGGCAGGGTAGTTCCCTGCTGTTTCatttattt                                                     <  2:3368566/96‑1 (MQ=255)
                                            tGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCa                                                   <  1:89517/100‑1 (MQ=255)
                                            tGCTTTTTCAGGGACGACAAAAAAAAAACAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCa                                                   <  2:2381723/100‑1 (MQ=255)
                                             gCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAg                                                  >  2:207876/1‑100 (MQ=255)
                                              cTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTATCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGa                                                 >  2:1746323/1‑100 (MQ=255)
                                              cTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGa                                                 >  1:3475786/1‑100 (MQ=255)
                                              cTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGa                                                 >  2:658851/1‑100 (MQ=255)
                                               tttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTTCTGTTTAatttattt                                                     >  2:3514540/1‑95 (MQ=255)
                                               tttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAt                                                >  1:1657729/1‑100 (MQ=255)
                                               tttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAt                                                >  1:1954873/1‑100 (MQ=255)
                                               tttttCAGGGACGACAACATACCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAt                                                <  1:3203438/100‑1 (MQ=255)
                                                ttttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAtt                                               >  2:10904/1‑100 (MQ=255)
                                                 tttCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttc                                              >  1:2142820/1‑100 (MQ=255)
                                                   tCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctt                                            <  2:97225/100‑1 (MQ=255)
                                                    cAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttcttt                                           >  1:545627/1‑100 (MQ=255)
                                                    cAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttcttt                                           >  1:3445947/1‑100 (MQ=255)
                                                     aGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttc                                          >  1:1254001/1‑100 (MQ=255)
                                                     aGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTACCTGCTGTTTCATTTATTTCAGAttctttc                                          >  2:3718456/1‑100 (MQ=255)
                                                      gggACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGAttctttct                                         <  1:257339/100‑1 (MQ=255)
                                                          cgacACCATCCCTACAAAAAATGCATATTGTTTTAGCGCGCGACCATTAGCTGGCATGGTACTTCCCTCACGTCTCATCTATTTCAGAatctttc                                          >  2:2384356/1‑95 (MQ=255)
                                                          cgacAACATTCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAat                                      <  2:3676321/99‑1 (MQ=255)
                                                          cgacAACATGCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTaa                                       <  2:847462/98‑1 (MQ=255)
                                                             caacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAattatt                                  >  2:3407567/1‑100 (MQ=255)
                                                              aacaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTAttt                                 <  1:128435/100‑1 (MQ=255)
                                                               acaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTAtttt                                >  2:1237747/1‑100 (MQ=255)
                                                               acaTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGCTTCATTTATTTCAGATTCTTTCTAATTAtttt                                <  1:3958948/100‑1 (MQ=255)
                                                                caTCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTc                               <  2:2689342/100‑1 (MQ=255)
                                                                 aTCCCTTCAAAAAATGCATATTGTTTTAGTGCGTGATTATTAGCTCGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGCTTCTTTCTAATTATTTTcc                              <  2:1615873/100‑1 (MQ=255)
                                                                  tCCCTGCAAAAAATGCATATTGTTTTAGAGCTTGATTATTATCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc                             <  2:3844014/100‑1 (MQ=255)
                                                                  tCCCTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc                             <  2:1277621/100‑1 (MQ=255)
                                                                    ccTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCg                           <  2:1470827/100‑1 (MQ=255)
                                                                     cTGCAAAGAATGCATATTGTTTTAGTGTGTGATTATTAGATGGCAGGGTAGTTCCTTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc                             <  2:139285/97‑1 (MQ=255)
                                                                     cTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTcccc                            <  2:3389341/98‑1 (MQ=255)
                                                                     cTGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTccc                             >  2:859520/1‑97 (MQ=255)
                                                                      tGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGTTGGCAAGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAg                         <  2:476695/100‑1 (MQ=255)
                                                                      tGCAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAg                         <  2:732529/100‑1 (MQ=255)
                                                                       gCAAAGAATGCATATTGTTTTAGAGCGTGATTATTAGCCGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTcccc                            <  2:668572/96‑1 (MQ=255)
                                                                        cAAAAAATGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCa                       <  2:2064815/100‑1 (MQ=255)
                                                                         aaaaaaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCaa                      >  1:3299040/1‑100 (MQ=255)
                                                                             aaTGCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTAc                  >  1:920266/1‑100 (MQ=255)
                                                                                gCATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTg               >  1:1751194/1‑100 (MQ=255)
                                                                                 cATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTgg              >  2:3640235/1‑100 (MQ=255)
                                                                                 cATATTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTgg              <  2:1883646/100‑1 (MQ=255)
                                                                                  atatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGc             >  1:2360580/1‑100 (MQ=255)
                                                                                  atatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGc             <  1:1079395/100‑1 (MQ=255)
                                                                                   tatTGTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCa            <  1:2210251/100‑1 (MQ=255)
                                                                                       gTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCTCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGATCAATTACTTggtcgct         >  2:549759/1‑94 (MQ=255)
                                                                                       gTTTTAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATc        >  2:4007772/1‑100 (MQ=255)
                                                                                          ttAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGATCAATTACGTGTCAGATCttt     >  2:2342522/1‑100 (MQ=255)
                                                                                          ttAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCttt     <  1:1181693/100‑1 (MQ=255)
                                                                                           tAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCtttt    <  1:1105979/100‑1 (MQ=255)
                                                                                           tAGAGCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCtttt    >  1:2449009/1‑100 (MQ=255)
                                                                                            agagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTc   <  1:3699501/100‑1 (MQ=255)
                                                                                             gagCGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCt  >  1:1490681/1‑100 (MQ=255)
                                                                                                |                                                                                                
TGCGGGTCAGATGTAATGATTCACTCATTCCTTTTCTCCATTTTTGCTTTTTCAGGGACGACAACATCCCTGCAAAAAATGCATATTGTTTTAGAGTGTGATTATTAGCTGGCAGGGTAGTTCCCTGCTGTTTCATTTATTTCAGATTCTTTCTAATTATTTTCCCCGAGCAATTACGTGGCAGATCTTTTCT  >  NC_000913/36135‑36327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: