Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,392 A→G 77.9% intergenic (+327/+46) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,3920AG77.9% 297.6 / 76.6 154intergenic (+327/+46)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base G (52/67);  total (68/86)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 155 total aligned reads displayed.

TCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAAT  >  NC_000913/2229293‑2229490
                                                                                                   |                                                                                                  
tCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTa                                                                                                    >  1:1961059/1‑100 (MQ=255)
  ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGtt                                                                                                  <  1:1513625/100‑1 (MQ=255)
  ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGtt                                                                                                  <  1:657226/100‑1 (MQ=255)
   cTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTg                                                                                                 >  1:3565016/1‑100 (MQ=255)
   cTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGCCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTg                                                                                                 <  2:2219533/100‑1 (MQ=255)
    tGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGa                                                                                                <  2:32153/100‑1 (MQ=255)
    tGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGa                                                                                                <  1:2433128/100‑1 (MQ=255)
     gTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGAt                                                                                               <  2:657976/100‑1 (MQ=255)
     gTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGAt                                                                                               >  1:2856513/1‑100 (MQ=255)
      tAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATa                                                                                              <  1:967226/100‑1 (MQ=255)
       aaaaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATaa                                                                                             <  1:32033/100‑1 (MQ=255)
         accgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAag                                                                                           <  2:3749244/98‑1 (MQ=255)
           cgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAagag                                                                                         <  1:389218/100‑1 (MQ=255)
           cgcgCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAagag                                                                                         >  1:1106996/1‑100 (MQ=255)
              gcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTa                                                                                      <  1:1836680/100‑1 (MQ=255)
              gcCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTa                                                                                      <  1:418040/100‑1 (MQ=255)
                ccTCAGCCCACATCCATGTGGGTTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAac                                                                                    >  2:2078979/1‑100 (MQ=255)
                ccTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAATCAACCGTGCGGCCTGTTTATAAAGAGCTacc                                                                                    <  1:2806898/100‑3 (MQ=18)
                  tCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAacac                                                                                  >  1:421429/1‑100 (MQ=255)
                   cAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAAcaca                                                                                 >  1:715769/1‑100 (MQ=21)
                   cAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAAcaca                                                                                 >  1:3469048/1‑100 (MQ=21)
                   cAGCCCACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAAcaca                                                                                 >  1:1589886/1‑100 (MQ=255)
                   cAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAAcaca                                                                                 <  1:3764044/100‑1 (MQ=255)
                     gCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACAtt                                                                               <  1:1604376/100‑1 (MQ=255)
                      cccACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTg                                                                              >  2:2956402/1‑100 (MQ=255)
                      cccACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTg                                                                              >  2:872999/1‑100 (MQ=255)
                        cacaTCCATGTGGGCTGCCCCGGCCTTCAGGGAACGACTCGGCAATGTTGACGCCACCAAACAGCCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTc                                                                            <  1:2933631/100‑1 (MQ=25)
                            tCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACACCCGTGCGGCCTGTTGATAAAGAGCTAACAGATTGTCaaa                                                                         >  2:290527/1‑99 (MQ=255)
                            tCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaa                                                                        <  1:391205/100‑1 (MQ=255)
                             ccATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaaa                                                                       <  1:2129384/100‑1 (MQ=255)
                               aTGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAAc                                                                     <  2:160570/100‑1 (MQ=255)
                               aTGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAAc                                                                     <  2:1589877/100‑1 (MQ=255)
                                tgtgGGCTGCCCCGGTCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACa                                                                    <  1:4104292/100‑1 (MQ=255)
                                tgtgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACa                                                                    <  1:456230/100‑1 (MQ=255)
                                  tgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATc                                                                  <  1:3366415/100‑1 (MQ=255)
                                  tgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATc                                                                  <  2:1657070/100‑1 (MQ=255)
                                  tgGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATc                                                                  >  1:3012118/1‑100 (MQ=255)
                                    ggCTGCCCCGGCCTTCAGGGAACGCCTCGGAAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCAc                                                                <  2:3341991/100‑1 (MQ=255)
                                     gCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACt                                                               >  2:3805056/1‑100 (MQ=255)
                                       tGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTAt                                                             <  1:3438389/100‑1 (MQ=255)
                                        gCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATg                                                            >  1:964090/1‑100 (MQ=255)
                                        gCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATg                                                            >  1:1358045/1‑100 (MQ=255)
                                             ggCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttt                                                       >  2:2385346/1‑100 (MQ=255)
                                               ccTTCAGGGAACGCCTCGGCAATTTTGACGCCACCCAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttt                                                       >  2:2250967/1‑98 (MQ=255)
                                                cTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTa                                                    >  1:2456695/1‑100 (MQ=255)
                                                 ttCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTag                                                   <  2:2037267/100‑1 (MQ=255)
                                                 ttCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTag                                                   <  2:2297891/100‑1 (MQ=255)
                                                  tCAGGGAGCGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGCTAAAGAGCTACGACATTGTCAAAAGACATCACTATGGTGATTTa                                                    >  2:2190637/1‑98 (MQ=37)
                                                  tCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTaga                                                  >  2:3257858/1‑100 (MQ=255)
                                                  tCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTaga                                                  >  1:1104758/1‑100 (MQ=255)
                                                   cAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTagag                                                 <  2:3555059/100‑1 (MQ=255)
                                                    aGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGt                                                >  1:2566263/1‑100 (MQ=255)
                                                     gggAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGtt                                               >  2:3010743/1‑100 (MQ=255)
                                                      ggAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGttt                                              >  2:2446897/1‑100 (MQ=255)
                                                       gAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTtc                                             >  2:196422/1‑100 (MQ=255)
                                                       gAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTAAAAAAACATCACTATGGTTTTTTAGAGTTtc                                             >  2:1181646/1‑100 (MQ=255)
                                                        aaCGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTtct                                            <  2:4078114/100‑1 (MQ=255)
                                                         aCGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTtctc                                           <  1:3786477/100‑1 (MQ=255)
                                                          cGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCg                                          >  2:407998/1‑100 (MQ=255)
                                                          cGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCg                                          <  1:3046845/100‑1 (MQ=255)
                                                          cGCCTCGGCAATTTTAACGCAACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACACTCTCAAAAAACATCACTATGATTTTTTACACTATCTCg                                          >  2:2553356/1‑100 (MQ=255)
                                                            ccTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGat                                        <  2:588257/100‑1 (MQ=255)
                                                             cTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGata                                       >  1:194668/1‑100 (MQ=255)
                                                              tCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGatan                                      <  2:2091059/100‑2 (MQ=255)
                                                               cGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATc                                     <  2:2087699/100‑1 (MQ=255)
                                                               cGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATc                                     <  2:2632581/100‑1 (MQ=255)
                                                               cGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATc                                     <  1:779389/100‑1 (MQ=255)
                                                                 gCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCaa                                   >  1:1970085/1‑100 (MQ=255)
                                                                  cAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAAt                                  >  2:1747683/1‑100 (MQ=255)
                                                                  cAATTTTGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAAt                                  >  2:1104405/1‑100 (MQ=255)
                                                                     ttttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGc                               <  1:993041/100‑1 (MQ=255)
                                                                      tttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGcc                              <  2:1997073/100‑1 (MQ=255)
                                                                      tttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGcc                              <  2:1343849/100‑1 (MQ=255)
                                                                      tttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGcc                              <  1:773937/100‑1 (MQ=255)
                                                                      tttGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGcc                              <  2:646689/100‑1 (MQ=255)
                                                                       ttGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCt                             <  1:3415214/100‑1 (MQ=255)
                                                                        tGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAATACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                            <  2:2662365/100‑1 (MQ=255)
                                                                        tGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                            <  2:458645/100‑1 (MQ=255)
                                                                        tGACGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                            <  2:3900875/100‑1 (MQ=255)
                                                                        tGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTg                            <  2:663185/100‑1 (MQ=255)
                                                                          aCGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGaa                          <  1:3709789/100‑1 (MQ=255)
                                                                           cGCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAAt                         >  1:3872873/1‑100 (MQ=255)
                                                                            gCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATa                        >  1:2714691/1‑100 (MQ=255)
                                                                            gCCACCAAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATa                        >  2:1323260/1‑100 (MQ=255)
                                                                              caccaAACAACCGTGCGGTCTGTTGATAAAGATCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCCATATCAATTGCCTGAATAGc                      <  2:1199263/100‑1 (MQ=255)
                                                                               accaAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGcc                     >  2:3573759/1‑100 (MQ=255)
                                                                                ccaAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGccc                    >  1:1874434/1‑100 (MQ=255)
                                                                                ccaAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGccc                    <  1:730656/100‑1 (MQ=255)
                                                                                ccaAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGccc                    <  1:317547/100‑1 (MQ=255)
                                                                                 caAACAACCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCt                   >  2:618354/1‑100 (MQ=255)
                                                                                    acaacCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGc                <  2:2752251/100‑1 (MQ=255)
                                                                                    acaacCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTCTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGc                <  2:3012145/100‑1 (MQ=255)
                                                                                       acCGTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAat             <  1:618358/100‑1 (MQ=255)
                                                                                         cGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAatat           >  1:3528589/1‑100 (MQ=255)
                                                                                          gTGCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAatat           >  1:996620/1‑99 (MQ=255)
                                                                                          gTGCGGCCAGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATc          <  2:1672616/100‑1 (MQ=255)
                                                                                            gCGGCCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAg        <  1:2241937/100‑1 (MQ=255)
                                                                                             cGGTCTGTTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAgg       <  2:3309714/100‑1 (MQ=255)
                                                                                                  tATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAat  >  2:2831985/1‑100 (MQ=255)
                                                                                                  tATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATAGTTTTTTAGAGTTTCTCGATATCAATTGACTGAATAGCCCTTGCAATATCAGGGGAat  <  2:2486088/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAATTGCCTGAATAGCCCTTGCAATATCAGGGGAAT  >  NC_000913/2229293‑2229490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: