Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,269,044 T→C 74.1% F64S (TTC→TCC)  lpxT → lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,269,0440TC74.1% 217.6 / 79.8 135F64S (TTC→TCC) lpxTlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (20/15);  new base C (49/50);  total (70/65)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

Only 100 of 135 total aligned reads displayed.

TGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCT  >  NC_000913/2268948‑2269140
                                                                                                |                                                                                                
tGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTc                                                                                                 >  2:1590260/1‑98 (MQ=255)
  ccGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACg                                                                                             >  1:1058039/1‑100 (MQ=255)
  ccGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACg                                                                                             >  1:771706/1‑100 (MQ=255)
      ttGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGGCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGCCCAACACTCGCGCCTCCGACGgttg                                                                                         <  2:823526/100‑1 (MQ=255)
         aTGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTc                                                                                      <  1:1363172/100‑1 (MQ=255)
          tGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCCACGGTTGTTCa                                                                                     >  2:526255/1‑100 (MQ=255)
           gCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCAc                                                                                    >  2:2071145/1‑100 (MQ=255)
              gATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCActgc                                                                                 <  1:211130/100‑1 (MQ=255)
                 atTTGTTATTTCTTTAATCAGAGACTGGTTGAAAGTAAGGCCTTTTTGTGGCTGGTTACATTGACCAACAATCGCGCCTCCGACGGTTGTTTACTGCTgg                                                                              <  2:4105856/100‑1 (MQ=255)
                  ttttttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGc                                                                             <  1:2108154/100‑1 (MQ=255)
                  ttttttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGc                                                                             <  2:3908945/100‑1 (MQ=255)
                     tttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGAt                                                                          <  1:3051123/100‑1 (MQ=255)
                     tttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGAt                                                                          <  1:3719228/100‑1 (MQ=255)
                     tttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGAt                                                                          <  2:2321793/100‑1 (MQ=255)
                      ttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATg                                                                         <  2:673906/100‑1 (MQ=255)
                      ttATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATg                                                                         <  2:3617926/100‑1 (MQ=255)
                       tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATgg                                                                        <  1:463165/100‑1 (MQ=255)
                       tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATgg                                                                        <  1:3442205/100‑1 (MQ=255)
                       tATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATgg                                                                        >  1:572325/1‑100 (MQ=255)
                        aTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATggg                                                                       <  1:4043733/100‑1 (MQ=255)
                        aTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATggg                                                                       <  1:3464317/100‑1 (MQ=255)
                        aTTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACAAACAATCGCGCCTCCGACGGTTGTTAACGGCTGGCGATggg                                                                       >  2:3435955/1‑100 (MQ=255)
                         tttCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGt                                                                      >  1:1008527/1‑100 (MQ=255)
                         tttCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTGTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATggg                                                                       <  2:2776695/99‑1 (MQ=255)
                           tCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTAt                                                                    >  1:1264231/1‑100 (MQ=255)
                            cTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATg                                                                   >  1:1928371/1‑100 (MQ=255)
                              ttAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGtt                                                                 <  1:3756501/100‑1 (MQ=255)
                              ttAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGtt                                                                 >  1:1911511/1‑100 (MQ=255)
                                aaTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGa                                                               <  1:3374181/100‑1 (MQ=255)
                                 aTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGAt                                                              >  2:925853/1‑100 (MQ=255)
                                 aTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGAt                                                              <  2:473773/100‑1 (MQ=255)
                                 aTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGAt                                                              >  2:23712/1‑100 (MQ=255)
                                 aTCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGAt                                                              >  1:1252596/1‑100 (MQ=255)
                                  tCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATg                                                             >  1:1794474/1‑100 (MQ=255)
                                  tCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATg                                                             <  1:1685659/100‑1 (MQ=255)
                                   cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGc                                                            <  2:2969153/100‑1 (MQ=255)
                                   cAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGc                                                            >  2:546854/1‑100 (MQ=255)
                                    aGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCt                                                           >  1:316554/1‑100 (MQ=255)
                                     gAAACTGGTCGAAAGTAAGGCCTTTTTGTTTTTGGTTTCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTg                                                          >  2:1827354/1‑100 (MQ=255)
                                         cTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGtt                                                      >  2:237753/1‑100 (MQ=255)
                                         cTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTACATTGACCAACAATCGCGCCTCCGACGGTTGTTCACGGCTGGCGATGGGTATGTTGATGCTGAGtt                                                      >  2:2509419/1‑100 (MQ=255)
                                           ggTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTc                                                    >  2:2293147/1‑100 (MQ=255)
                                           ggTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTc                                                    >  2:170520/1‑100 (MQ=255)
                                            gTCGAAAGTAAGGCCTTTTTGTTTCTTTTTTCATTGAACAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCt                                                   >  2:2723066/1‑100 (MQ=255)
                                               gAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGc                                                <  2:316551/100‑1 (MQ=255)
                                                 aaGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTg                                              <  2:13928/100‑1 (MQ=255)
                                                  aGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTga                                             <  2:2992790/100‑1 (MQ=255)
                                                  aGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGGTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTga                                             <  2:1598874/100‑1 (MQ=255)
                                                   gTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaa                                            >  2:1645614/1‑100 (MQ=255)
                                                   gTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaa                                            <  2:1329655/100‑1 (MQ=255)
                                                     aaGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaag                                          >  1:2351176/1‑100 (MQ=255)
                                                     aaGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaag                                          <  1:2071156/100‑1 (MQ=255)
                                                      aGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTgaaaga                                         <  1:2551290/100‑1 (MQ=255)
                                                         ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGaaaa                                      >  1:3491993/1‑100 (MQ=255)
                                                         ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGaaaa                                      >  1:696754/1‑100 (MQ=255)
                                                         ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGaaaa                                      <  1:3827372/100‑1 (MQ=255)
                                                         ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGAGTATGTTGATGCTGAGTTTCTGGCTGAAAGaaaa                                      >  2:3747120/1‑100 (MQ=255)
                                                         ccTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTGGTTCACTGCTGGCGATGGGTAAGTTGATGCTGAGTTTCTGGCTgataggaa                                       >  1:2595521/1‑99 (MQ=255)
                                                          cTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAAc                                     <  2:1291636/100‑1 (MQ=255)
                                                           tttttGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACg                                    <  2:2756930/100‑1 (MQ=255)
                                                            ttttGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGc                                   >  1:2222623/1‑100 (MQ=255)
                                                            ttttGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGc                                   <  2:1623519/100‑1 (MQ=255)
                                                            ttttGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGc                                   <  1:831603/100‑1 (MQ=255)
                                                               tgtgGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTGGTTCACTGCTGGCGAGGGGTATGTTTATGCTGAGTTTCGGGCTGAAAGAAAACGcccc                                >  1:3321976/1‑100 (MQ=255)
                                                                 tgGCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTg                              <  1:4087721/100‑1 (MQ=255)
                                                                  ggCTGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTgg                             <  2:3759543/100‑1 (MQ=255)
                                                                     tGGTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAg                          <  1:4011108/100‑1 (MQ=255)
                                                                      ggTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAga                         >  1:2150547/1‑100 (MQ=255)
                                                                      ggTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCa                           <  2:3442577/98‑1 (MQ=255)
                                                                       gTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgac                        >  2:3130687/1‑100 (MQ=255)
                                                                       gTTGCATTGACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgac                        >  1:1815155/1‑100 (MQ=255)
                                                                        tttcagtGACCAACAAGCGCGCCTCCTGCGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacg                       <  1:767062/94‑1 (MQ=255)
                                                                         tGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga                      >  1:3762984/1‑100 (MQ=255)
                                                                          gCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacgac                     >  2:1961304/1‑100 (MQ=255)
                                                                          gCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGATTCTGGCTGAAAGAAAACGCCCCTGGCAgacgac                     >  1:637110/1‑100 (MQ=255)
                                                                           cATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGacgacg                    >  1:1077400/1‑100 (MQ=255)
                                                                             ttGACCAACAATCGCGCTTTCGACGGTTGTTCTCTGCTGGCGATGGGAATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGacgacg                    <  2:1716316/98‑1 (MQ=255)
                                                                              tGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTAt                 >  1:3881982/1‑100 (MQ=255)
                                                                              tGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTAt                 <  2:3940524/100‑1 (MQ=255)
                                                                               gACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTAAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc                >  2:2919049/1‑100 (MQ=255)
                                                                               gACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc                >  1:797991/1‑100 (MQ=255)
                                                                               gACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc                <  1:3070770/100‑1 (MQ=255)
                                                                               gACCAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc                >  2:1958034/1‑100 (MQ=255)
                                                                                aCCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCg               <  1:925857/100‑1 (MQ=255)
                                                                                 ccAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGCGTTTCTGGCTGACAGAAAACGCCCCTGGCAGACGACGTATctt              >  2:4028944/1‑98 (MQ=255)
                                                                                 ccAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGt              <  2:3565920/100‑1 (MQ=255)
                                                                                 ccAACAATCGCGCCTCCGACGGTTGTTCACTGCTGGCGAGGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGt              <  2:532645/100‑1 (MQ=255)
                                                                                   aacaaTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGa            >  2:3950935/1‑100 (MQ=255)
                                                                                    acaaTCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGat           <  1:93928/100‑1 (MQ=255)
                                                                                    acaaTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGat           >  1:1796448/1‑100 (MQ=255)
                                                                                       aTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGGAAACGCCACTGGCAGACGACGTATCGTGattat        >  2:686565/1‑100 (MQ=255)
                                                                                       aTCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGattat        <  1:1571216/100‑1 (MQ=255)
                                                                                        tCGCGCCTCCGACGGTTGTTCACTGCTGGCGGTGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCGTGGCAGACGGCGTATCGTGattat        >  2:924464/1‑99 (MQ=255)
                                                                                        tCGCGCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGattatt       <  2:1264224/100‑1 (MQ=255)
                                                                                         cgcgCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtg      >  1:3363980/1‑100 (MQ=255)
                                                                                         cgcgCCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtg      >  1:7245/1‑100 (MQ=255)
                                                                                          gcgcCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtgg     <  2:2046668/100‑1 (MQ=255)
                                                                                           cgcCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggt    <  1:170523/100‑1 (MQ=255)
                                                                                            gcCTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtc   <  1:75383/100‑1 (MQ=255)
                                                                                             ccTCCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtct  >  1:3113034/1‑100 (MQ=255)
                                                                                                |                                                                                                
TGCCGATTGATGCGGATATTTTTTATTTCTTTAATCAGAAACTGGTCGAAAGTAAGGCCTTTTTGTGGCTGGTTGCATTGACCAACAATCGCGCCTTCGACGGTTGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCT  >  NC_000913/2268948‑2269140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: