BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,521,967	C→A	100%	A171S (GCG→TCG)	xapR ←	transcriptional activator of xapAB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,521,967	0	C	A	100.0%	490.6 / NA	145	A171S (GCG→TCG)	xapR	transcriptional activator of xapAB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (74/71); total (74/71)

Only 100 of 150 total aligned reads displayed.

GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTG  >  NC_000913/2521870‑2522066

ggcggcATTGTAAAAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg                                                                                                   <  1:1625455/100‑1 (MQ=255)

 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                                  >  2:3285298/1‑100 (MQ=255)

 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                                  <  2:40335/100‑1 (MQ=255)

 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                                  >  1:3219351/1‑100 (MQ=255)

   ggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAtt                                                                                                <  2:48298/100‑1 (MQ=255)

     cATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCa                                                                                              <  2:806629/100‑1 (MQ=255)

       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                            <  1:952844/100‑1 (MQ=255)

       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                            <  1:949361/100‑1 (MQ=255)

         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                          <  2:1468316/100‑1 (MQ=255)

         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                          >  1:302824/1‑100 (MQ=255)

         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                          >  2:856139/1‑100 (MQ=255)

         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                          <  1:3966010/100‑1 (MQ=255)

         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                          >  2:2283673/1‑100 (MQ=255)

          tAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCaa                                                                                         >  1:3064077/1‑100 (MQ=255)

            aaaaaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAGATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                       >  1:2818756/3‑100 (MQ=255)

            aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                       <  2:3770021/100‑1 (MQ=255)

             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                      <  2:3997/100‑1 (MQ=255)

             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                      >  1:899527/1‑100 (MQ=255)

             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                      >  1:927222/1‑100 (MQ=255)

               aaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTa                                                                                    <  1:1954243/100‑1 (MQ=255)

                aaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTaa                                                                                   >  2:1173070/1‑100 (MQ=255)

                   aCTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCt                                                                                <  1:2490936/100‑1 (MQ=255)

                    cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg                                                                               >  2:3130882/1‑100 (MQ=255)

                    cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg                                                                               >  1:2430149/1‑100 (MQ=255)

                    cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg                                                                               >  1:1680601/1‑100 (MQ=255)

                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                             >  1:43077/1‑100 (MQ=255)

                        tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaa                                                                           >  1:2424439/1‑100 (MQ=255)

                            tcgcttacgacAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                       <  1:2978722/90‑1 (MQ=255)

                            gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                       >  2:917537/1‑100 (MQ=255)

                            gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                       <  1:1203800/100‑1 (MQ=255)

                             tAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCa                                                                      >  1:1862456/1‑100 (MQ=255)

                              aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGTTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                     <  2:1498273/100‑1 (MQ=255)

                                cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc                                                                   >  1:2899622/1‑100 (MQ=255)

                                cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc                                                                   <  2:2696010/100‑1 (MQ=255)

                                 gcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCg                                                                  >  2:2626768/1‑100 (MQ=255)

                                  cTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgg                                                                 <  1:1323538/100‑1 (MQ=255)

                                   ttCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggt                                                                >  2:2534097/1‑100 (MQ=255)

                                   ttCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggt                                                                >  2:1798422/1‑100 (MQ=255)

                                    tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtg                                                               <  1:2639467/100‑1 (MQ=255)

                                    tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCgggg                                                               >  2:2533000/1‑98 (MQ=255)

                                    tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCCTAAGCTGGTAAAACCAGTCggtg                                                               >  2:1642164/1‑100 (MQ=255)

                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                             >  2:492949/1‑100 (MQ=255)

                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                             <  2:3186697/100‑1 (MQ=255)

                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtgct                                                             <  1:2808855/100‑3 (MQ=255)

                                        gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                             <  2:2745096/98‑1 (MQ=255)

                                        gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                           <  1:2491258/100‑1 (MQ=255)

                                           ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt                                                        >  1:2095790/1‑100 (MQ=255)

                                           ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt                                                        <  1:1305129/100‑1 (MQ=255)

                                           ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAGTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt                                                        <  2:2452948/100‑1 (MQ=255)

                                            gACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTc                                                       <  2:1376203/100‑1 (MQ=255)

                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                    >  2:594818/1‑100 (MQ=255)

                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                    <  2:3868566/100‑1 (MQ=255)

                                                gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                                   >  1:3270777/1‑100 (MQ=255)

                                                 tGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAt                                                  >  2:2790470/1‑100 (MQ=255)

                                                 tGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAt                                                  >  1:3360870/1‑100 (MQ=255)

                                                    aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCg                                               <  1:3259128/100‑1 (MQ=255)

                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                             <  2:3834200/100‑1 (MQ=255)

                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                             <  2:1308534/100‑1 (MQ=255)

                                                       atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa                                            >  1:666418/1‑100 (MQ=255)

                                                        tgatgaGGGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACAGTAAGCTGGTAAAACCAGTCGGTGGTTCTCACCTCATTCGCCa                                            >  2:2729014/1‑99 (MQ=255)

                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                           >  2:3671596/1‑100 (MQ=255)

                                                         gatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGa                                          <  1:484249/100‑1 (MQ=255)

                                                           tgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa                                            <  2:1863751/96‑1 (MQ=255)

                                                           tgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATc                                        >  2:2478793/1‑100 (MQ=255)

                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcgc                                      >  1:3962363/1‑98 (MQ=255)

                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc                                      >  2:2156153/1‑100 (MQ=255)

                                                              gagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCg                                     <  2:666414/100‑1 (MQ=255)

                                                              gagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCg                                     >  1:643710/1‑100 (MQ=255)

                                                               agaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg                                    <  1:4039116/100‑1 (MQ=255)

                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                                   <  2:3464491/100‑1 (MQ=255)

                                                                 atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa                                  <  2:3571244/100‑1 (MQ=255)

                                                                  tgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCAt                                 >  1:3449557/1‑100 (MQ=255)

                                                                   gatgCTCTTCAGGCTTCGCCCCCAGAACCAACGATTCATGCACCCGTAAGTTGGTAAAACCAGTCAGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                                <  2:3632922/100‑1 (MQ=255)

                                                                   gatgCTCTTCAGGCATCGCCAACAGAAACGACGATTCAGGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                                >  2:2564563/1‑100 (MQ=255)

                                                                      gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                             <  1:1953149/100‑1 (MQ=255)

                                                                            cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                       >  1:3327487/1‑100 (MQ=255)

                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                      <  1:2223177/100‑1 (MQ=255)

                                                                              ggCATCGCCACCAGAAACGACGATTCATGCACCCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc                     <  2:2402533/100‑1 (MQ=255)

                                                                               gCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTTGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                      <  2:3464860/98‑1 (MQ=255)

                                                                               gCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggcg                    <  2:3270754/100‑1 (MQ=255)

                                                                               gCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggcg                    <  2:1071557/100‑1 (MQ=255)

                                                                                cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGt                   >  1:3476673/1‑100 (MQ=255)

                                                                                 aTCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGtt                  <  1:3149681/100‑1 (MQ=255)

                                                                                 aTCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGtt                  <  1:1143961/100‑1 (MQ=255)

                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa               <  2:3035673/100‑1 (MQ=255)

                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa               >  1:4072728/1‑100 (MQ=255)

                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGCAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa               >  2:2015917/1‑100 (MQ=255)

                                                                                     ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCan              <  2:3323849/100‑2 (MQ=255)

                                                                                     ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg              >  2:2995011/1‑100 (MQ=255)

                                                                                     ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCCCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg              <  2:2493519/100‑1 (MQ=255)

                                                                                       accaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa            <  1:259014/100‑1 (MQ=255)

                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa           >  2:3692860/1‑100 (MQ=255)

                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCa            <  1:1828238/99‑1 (MQ=255)

                                                                                         caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg          >  1:3764421/1‑100 (MQ=255)

                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg         <  1:1067872/100‑1 (MQ=255)

                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg         <  1:3535941/100‑1 (MQ=255)

                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg         <  2:2033713/100‑1 (MQ=255)

                                                                                           gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc        >  2:1766126/1‑100 (MQ=255)

                                                                                            naaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCCGCAAGGcc       >  1:1008190/2‑100 (MQ=255)

                                                                                                 ccGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTg  >  2:775377/1‑100 (MQ=255)

GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCATTTG  >  NC_000913/2521870‑2522066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`