Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 428,188 G→T 100% R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,1880GT98.5% 497.4 / ‑2.8 151R181L (CGC→CTC) secDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (62/85);  total (64/87)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

CAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428091‑428283
                                                                                                 |                                                                                               
cAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCctcg                                                                                               <  1:2226854/100‑1 (MQ=255)
cAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCctcg                                                                                               >  2:179492/1‑100 (MQ=255)
 aaaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGa                                                                                              <  2:1514832/100‑1 (MQ=255)
 aaaaTATCGACAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGa                                                                                              >  2:40036/1‑100 (MQ=255)
  aaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCctcg                                                                                               >  2:3216026/1‑98 (MQ=255)
   aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg                                                                                            <  1:1020127/100‑1 (MQ=255)
    atatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc                                                                                           <  1:3091003/100‑1 (MQ=255)
     tatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCt                                                                                          <  2:560811/100‑1 (MQ=255)
       tCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTaa                                                                                        <  2:4171005/100‑1 (MQ=255)
        cGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTaaa                                                                                       >  1:3846736/1‑100 (MQ=255)
         gATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                      >  1:2267010/1‑100 (MQ=255)
         gATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                      >  1:591084/1‑100 (MQ=255)
          aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGc                                                                                     <  1:2547690/100‑1 (MQ=255)
          aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGc                                                                                     <  1:3216047/100‑1 (MQ=255)
          aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGc                                                                                     <  1:2491162/100‑1 (MQ=255)
            aGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTc                                                                                   >  1:705781/1‑100 (MQ=255)
             gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg                                                                                  >  1:3752620/1‑100 (MQ=255)
             gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg                                                                                  >  1:2946022/1‑100 (MQ=255)
             gCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCg                                                                                  >  1:1564773/1‑100 (MQ=255)
              tcTACGCAGTGACCTGCGCGAGAAGAGCATGCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCGTCACTTTCCTCGATGCTATAGCTCGt                                                                                 >  2:2456072/2‑100 (MQ=255)
              ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 <  1:4051023/100‑1 (MQ=255)
              ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 <  2:891050/100‑1 (MQ=255)
               cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  1:1573977/100‑1 (MQ=255)
               cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  2:2689660/100‑1 (MQ=255)
                tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                               <  1:1747732/100‑1 (MQ=255)
                tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                               >  1:3248768/1‑100 (MQ=255)
                 aCGCAGTGTCCGGCGCGCAAAGGGCTTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCTtga                                                                               <  2:3407463/99‑1 (MQ=255)
                 aCGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgat                                                                              >  1:466548/1‑100 (MQ=255)
                   gCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatga                                                                            <  2:591080/100‑1 (MQ=255)
                   gCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTCTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatga                                                                            <  2:2266996/100‑1 (MQ=255)
                    cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGaa                                                                           <  1:2370080/100‑1 (MQ=255)
                     aGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                          <  1:582399/100‑1 (MQ=255)
                      gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                         <  1:3474123/100‑1 (MQ=255)
                          ccggcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     <  1:3565269/97‑1 (MQ=255)
                          ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     >  2:2955982/1‑100 (MQ=255)
                           cTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTg                                                                    <  2:2134324/100‑1 (MQ=255)
                           cTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGACCATCACTTTCCTCGATGCTAAAGCTCGTGATGACGc                                                                         >  2:2148682/1‑95 (MQ=255)
                            tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACACCTACGGCCTTAGCATCACTTCCCTCTATGCTAAAGCTCGTGATGCAGcc                                                                        <  2:2202114/95‑1 (MQ=255)
                            tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                   <  2:1008687/100‑1 (MQ=255)
                            tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                   <  1:194153/100‑1 (MQ=255)
                             gcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGTATCACTTTCCTCGATGCTAAAGCTCGTGTTGAAGCCATTggg                                                                  <  1:2823100/100‑3 (MQ=255)
                             gcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCg                                                                  <  1:629096/100‑1 (MQ=255)
                               gcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                                >  2:673540/1‑100 (MQ=255)
                                cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                               >  1:77381/1‑100 (MQ=255)
                                cgAAAAGGGCATCCCGTATACCACTGTTCAGAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                                <  2:1768657/99‑1 (MQ=255)
                                 gAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                              <  2:1687795/100‑1 (MQ=255)
                                 gAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                              >  1:2646228/1‑100 (MQ=255)
                                   aaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg                                                            >  1:1995157/1‑100 (MQ=255)
                                   aaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg                                                            >  2:3173289/1‑100 (MQ=255)
                                     aGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAg                                                          <  2:3806802/100‑1 (MQ=255)
                                       ggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                        <  1:2993095/100‑1 (MQ=255)
                                         cATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCCGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAg                                                      >  1:1417933/1‑100 (MQ=255)
                                          aTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTACGCAAgc                                                     >  2:3803580/1‑100 (MQ=255)
                                            cccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcgc                                                   <  1:673544/100‑1 (MQ=255)
                                            cccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcgc                                                   <  1:745633/100‑1 (MQ=255)
                                             ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGcc                                                  <  1:3556986/100‑1 (MQ=255)
                                              cGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCa                                                 >  1:333162/1‑100 (MQ=255)
                                               gTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCAt                                                <  2:3017988/100‑1 (MQ=255)
                                                tataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGccttc                                               >  2:1930040/1‑97 (MQ=255)
                                                   aCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCgg                                            <  2:3349659/100‑1 (MQ=255)
                                                      aCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                         <  2:1522369/100‑1 (MQ=255)
                                                       cTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCt                                        <  2:254580/100‑1 (MQ=255)
                                                         gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                      >  2:4050263/1‑100 (MQ=255)
                                                          ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  2:1995147/100‑1 (MQ=255)
                                                           tCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  2:3261606/99‑1 (MQ=255)
                                                           tCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTg                                    <  2:633745/100‑1 (MQ=255)
                                                             gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:3302612/100‑1 (MQ=255)
                                                             gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:2614315/100‑1 (MQ=255)
                                                             gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  1:1284606/100‑1 (MQ=255)
                                                              tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 >  2:527248/1‑100 (MQ=255)
                                                               acgcaaagCAACTAGGGCCTGAGCTTCACTTTCCTCGATGATAAGGCGCCTGGCGAAGCCGTTACGTACCTGAGCAAGCGCCATCCGGACCTgg                                      >  2:3962022/5‑94 (MQ=255)
                                                               aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa                                >  1:1501208/1‑100 (MQ=255)
                                                               aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa                                >  2:325412/1‑100 (MQ=255)
                                                                aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               <  1:3905211/100‑1 (MQ=255)
                                                                aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               >  2:1690244/1‑100 (MQ=255)
                                                                aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               <  1:683974/100‑1 (MQ=255)
                                                                 aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                              <  2:40570/100‑1 (MQ=255)
                                                                 aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                              >  1:1310027/1‑100 (MQ=255)
                                                                 aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGATCGTGATGAAGCAATTGCGTATCTGAGCAAGCGCCATCCGAACCTGGTGATTagc                              >  1:2054559/1‑100 (MQ=255)
                                                                       caacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAg                        >  1:3019425/1‑100 (MQ=255)
                                                                          cTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAgggn                     <  1:1495048/100‑2 (MQ=255)
                                                                           tACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgca                    <  1:3770683/100‑1 (MQ=255)
                                                                           tACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgc                     <  1:1698403/99‑1 (MQ=255)
                                                                              ggCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca                 <  1:3523599/100‑1 (MQ=255)
                                                                               gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCaa                >  1:1427928/1‑100 (MQ=255)
                                                                                ccTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAc               <  1:653038/100‑1 (MQ=255)
                                                                                 cTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc              <  2:775930/100‑1 (MQ=255)
                                                                                 cTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc              <  1:3163293/100‑1 (MQ=255)
                                                                                  tGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCa             >  1:3673869/1‑100 (MQ=255)
                                                                                      cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         >  2:1406531/1‑100 (MQ=255)
                                                                                      cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         <  1:857735/100‑1 (MQ=255)
                                                                                       aTCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgc        >  1:2204854/1‑100 (MQ=255)
                                                                                       aTCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgc        >  1:636465/1‑100 (MQ=255)
                                                                                        tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg       <  1:1140555/100‑1 (MQ=255)
                                                                                        tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg       >  1:793925/1‑100 (MQ=255)
                                                                                        tCACTTTCCTAGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg       <  2:2743846/100‑1 (MQ=255)
                                                                                           cTTTCCTCGATGCTGAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    <  2:1597287/100‑1 (MQ=255)
                                                                                           cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    <  1:2469798/100‑1 (MQ=255)
                                                                                           cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    <  2:234558/100‑1 (MQ=255)
                                                                                           cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    <  1:85170/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CAAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428091‑428283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑