BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,931	T→C	22.5%	intergenic (‑71/+128)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,931	0	T	C	22.5%	282.2 / 93.1	169	intergenic (‑71/+128)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (67/63); new base C (19/19); total (86/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 171 total aligned reads displayed.

ACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATA  >  NC_000913/2817835‑2818030

aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCg                                                                                                  >  1:1025921/1‑100 (MQ=255)

 ctctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCgg                                                                                                 >  2:3010988/1‑100 (MQ=255)

  tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCggg                                                                                                >  2:4046/1‑100 (MQ=255)

  tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCggg                                                                                                <  1:3491568/100‑1 (MQ=255)

      tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggagn                                                                                             <  2:3711570/99‑2 (MQ=255)

      tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggagga                                                                                            <  1:2201312/100‑1 (MQ=255)

         accacggCTACCGAAGCAT‑GGGAAAATTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAttcg                                                                                        <  2:1571138/94‑3 (MQ=11)

             gAGCTACGGATGCATCGGGAAAACTTCTTCTACTGCTGATATTGATACCGCTACCAAACCCATATCAATAAAGAGATGTTCCATCCGGGAGGATTAcac                                                                                      >  2:3574893/1‑97 (MQ=17)

             gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATTGTGAATCCGGTAGGATTagaaa                                                                                     >  2:153266/1‑96 (MQ=17)

             gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCg                                                                                     >  1:1655612/1‑100 (MQ=255)

               ncTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc                                                                                   >  2:1196836/2‑100 (MQ=255)

               gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc                                                                                   <  1:1614368/100‑1 (MQ=255)

               gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGcc                                                                                   >  1:1720808/1‑100 (MQ=255)

               gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGc                                                                                    >  2:70213/1‑99 (MQ=255)

                 tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCtt                                                                                 >  1:496257/1‑100 (MQ=255)

                 naCGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                        >  1:1232010/2‑93 (MQ=255)

                  aCGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                >  1:327475/1‑100 (MQ=255)

                    ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                              <  2:3500889/100‑1 (MQ=255)

                        gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGTTGGTGCACCCTGGAGGATTGCTAGCATTGCgtc                                                                            >  2:2255152/1‑96 (MQ=17)

                         cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                             >  2:957370/1‑96 (MQ=255)

                         cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                         >  2:3151091/1‑100 (MQ=255)

                         cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTACATCCGAAAAGCTTACTctacttaaag                                                                             >  2:1958919/1‑87 (MQ=17)

                          aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                        >  1:2870513/1‑100 (MQ=255)

                          aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                        >  1:3153171/1‑100 (MQ=255)

                          aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                        <  1:2379423/100‑1 (MQ=255)

                          aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                        <  2:2337522/100‑1 (MQ=255)

                          aTCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                        <  1:782969/100‑1 (MQ=255)

                           tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGc                                                                            >  1:979460/1‑95 (MQ=255)

                            cGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTAGc                                                                        >  1:1571147/1‑98 (MQ=255)

                            cGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                      >  2:773708/1‑100 (MQ=255)

                             gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                     >  1:1719815/1‑100 (MQ=255)

                             gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCt                                                                     >  1:3529133/1‑100 (MQ=255)

                              ggAAAACTTATTATACTGCTGATATTGATACCGCTACTAACGCCATATCACTTAAGAGATGGTGCATCCTGGAGGATTACTCGCCCTGCGGATCGCCCt                                                                     >  2:304162/1‑99 (MQ=18)

                                 aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                 <  1:1004044/100‑1 (MQ=255)

                                 aaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                 <  2:2870497/100‑1 (MQ=255)

                                   aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                               >  2:2494063/1‑100 (MQ=255)

                                   aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                               <  2:3101713/100‑1 (MQ=255)

                                   aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATAAATTAAGAGATGATGAATCCGGGAGGATTACTCGCCATGCGGATCGACCTTCGGGc                                                               >  1:3477199/1‑100 (MQ=18)

                                    cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGcc                                                              >  2:1724765/1‑100 (MQ=255)

                                      tATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGt                                                            >  2:3974627/1‑100 (MQ=255)

                                      tATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCg                                                             >  2:1124477/1‑99 (MQ=255)

                                       aTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                           <  1:2733459/100‑1 (MQ=255)

                                        ttCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                          <  1:2616986/100‑1 (MQ=255)

                                         tCTACTGCTGATATTGATACCGTTACTAAAGCCATATCTATTAAGAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGc                                                         <  1:2462574/100‑1 (MQ=255)

                                          cTACTGGTGATATTGATACCGCTACTAAAGCCATATCAATTCAGAGATGGTGCCTCCGGGAGGCTTACTCCCCTTGCGGCTCGCCCTTCGGGCCGTTGCt                                                        <  2:1868930/100‑1 (MQ=17)

                                            aCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaa                                                      >  1:975091/1‑100 (MQ=255)

                                              tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCTCGCCTTGCGGCTCGCCCTTGGGGCCGTGGCTAAAg                                                    >  1:1687815/1‑100 (MQ=255)

                                              tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAg                                                    <  1:692528/100‑1 (MQ=255)

                                               gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCGGtcggcacagc                                                   >  2:1555464/1‑91 (MQ=14)

                                                ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                                  <  1:1985187/100‑1 (MQ=255)

                                                 tgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                 >  1:389200/1‑100 (MQ=255)

                                                   atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACg                                               <  1:932699/100‑1 (MQ=40)

                                                    tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                              <  1:12552/100‑1 (MQ=40)

                                                    tatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCa                                                  >  2:1072413/1‑96 (MQ=255)

                                                     atTTATACTGCTACTAAAGCCATATTAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                             <  2:1983612/100‑1 (MQ=11)

                                                     atTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGtt                                             >  2:511482/1‑100 (MQ=40)

                                                      ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcc                                            <  1:736293/100‑4 (MQ=14)

                                                      ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTa                                            >  1:3792266/1‑100 (MQ=38)

                                                      ttGATACCGCTACCAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggcc                                            <  1:2421025/100‑4 (MQ=14)

                                                       tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTTTAGCAACggccc                                           <  1:1873628/100‑5 (MQ=11)

                                                       tGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                           <  2:2891020/100‑1 (MQ=255)

                                                        gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                           >  1:777266/1‑99 (MQ=38)

                                                        gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTACCCTTGCGCCTCCGCCTTCGGGCCGAGGCTACGGCAACGTTac                                           >  2:2769850/1‑98 (MQ=14)

                                                         agaCCGCTACTAAAGCAATATTAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatca                                         <  2:883606/98‑1 (MQ=255)

                                                           aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                       <  2:496254/100‑1 (MQ=255)

                                                              gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                    >  1:1764636/1‑100 (MQ=255)

                                                                tactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAg                                  <  1:3068366/100‑1 (MQ=255)

                                                                  ctaAAGCTATATCAATTAAGAGATGGGGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                <  1:3918883/100‑1 (MQ=255)

                                                                  ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                <  1:3217033/100‑1 (MQ=255)

                                                                   taAAGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                               >  2:561986/1‑100 (MQ=255)

                                                                    aaaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGcc                              >  1:3930800/1‑100 (MQ=255)

                                                                      aGCCATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                            >  2:2627417/1‑100 (MQ=255)

                                                                       gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                           >  1:1137221/1‑100 (MQ=255)

                                                                       gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGc                           >  1:1304906/1‑100 (MQ=255)

                                                                        ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCg                          <  1:686828/100‑1 (MQ=255)

                                                                         cATATCAATTAAGAGATGGTGCACCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTGGTGCCTGCgg                         >  1:2842849/1‑100 (MQ=255)

                                                                          atatTAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                        <  1:3181584/100‑1 (MQ=255)

                                                                          atatCAATTAAGGGATGGTGCAGCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                        <  1:407155/100‑1 (MQ=255)

                                                                            atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTa                      >  2:625516/1‑100 (MQ=255)

                                                                             tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                     <  1:1338589/100‑1 (MQ=255)

                                                                              cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                    <  1:3643433/100‑1 (MQ=255)

                                                                              cAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                    >  2:1276799/1‑100 (MQ=255)

                                                                               aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGCCTCGCCCTTCGGGCCGTTGATAAAGCAACGCTATCATCCCTAGTGCCTGCGGTTAAtt                   >  2:2271073/1‑100 (MQ=255)

                                                                                aTTAAGACATCGTGCATCCGGGCGGATTACTCGCCTTGCGGCTCGCCCCTCGGGCCGGTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                  <  2:2136592/100‑1 (MQ=14)

                                                                                 ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTACAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                 <  1:2915453/100‑1 (MQ=255)

                                                                                   aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc               >  2:2850901/1‑100 (MQ=255)

                                                                                   aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc               <  2:3652967/100‑1 (MQ=255)

                                                                                   aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc               <  1:1485921/100‑1 (MQ=255)

                                                                                   aaGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCAAGTGCCTGCTGTTAATTc                  >  2:1850945/1‑97 (MQ=255)

                                                                                    agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt              >  2:3155466/1‑100 (MQ=255)

                                                                                    agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt              >  1:2483353/1‑100 (MQ=255)

                                                                                    agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt              <  2:1304899/100‑1 (MQ=255)

                                                                                      agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt              <  1:1221475/98‑1 (MQ=255)

                                                                                      agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc            <  2:2684609/100‑1 (MQ=255)

                                                                                       gaTGGTGCATCCGGGAGGATTCCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc           <  1:3708780/100‑1 (MQ=255)

                                                                                       gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc           <  2:4152631/100‑1 (MQ=255)

                                                                                        aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACct          <  2:2746204/100‑1 (MQ=255)

                                                                                         tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctc         >  1:2070769/1‑100 (MQ=255)

                                                                                           gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTAAAGCAACGGTGTCATCCCTAGTGCCTGTGGTTAATTCCGCTACATCtt       >  2:2022647/1‑100 (MQ=17)

                                                                                             gcgtCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc      <  2:378276/96‑1 (MQ=255)

ACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATA  >  NC_000913/2817835‑2818030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`