Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 475,614 A→G 15.5% D79G (GAT→GGT)  ybaY → outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913475,6140AG15.5% 341.2 / 55.6 157D79G (GAT→GGT) ybaYouter membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (70/61);  new base G (11/13);  total (82/75)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-03

Only 100 of 158 total aligned reads displayed.

CAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCC  >  NC_000913/475517‑475713
                                                                                                 |                                                                                                   
  aCCGAATGTCTCGGGTACCGTCTGGCTCCGTGAGAAAGTCGCGCTGCCGCCGGATGCTGTGCTGACCGTGCCAATTTCTGGCGCGTCGTTAGCCGATGCa                                                                                                 <  2:3449734/100‑1 (MQ=255)
  aCCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCa                                                                                                 <  2:2868774/100‑1 (MQ=255)
  aCCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACGTTCTGACGCGTCGTTATCCGATGc                                                                                                  >  2:1528063/1‑99 (MQ=255)
     gAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCg                                                                                              >  1:3331506/1‑100 (MQ=255)
      aaTGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGt                                                                                             <  2:740068/100‑1 (MQ=255)
           ctcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaaaa                                                                                        >  2:252857/1‑100 (MQ=255)
           ctcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTCTGCTGACCGTGCCACTTTCTGCCGCGTCGTTCGCCGCTGCACCGTCacac                                                                                        >  2:4167117/1‑97 (MQ=255)
            tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAg                                                                                       <  2:747112/100‑1 (MQ=255)
            tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAg                                                                                       <  1:3374118/100‑1 (MQ=255)
            tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAg                                                                                       >  2:3575638/1‑100 (MQ=255)
            tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAg                                                                                       <  2:1614628/100‑1 (MQ=255)
                  aCCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTgg                                                                                 <  1:2253514/100‑1 (MQ=255)
                  aCCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTgg                                                                                 <  1:2777390/100‑1 (MQ=255)
                  aCCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGATgg                                                                                 >  2:3659912/1‑100 (MQ=255)
                   ccGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGgc                                                                                >  1:2634684/1‑100 (MQ=255)
                    cGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGgcg                                                                               >  1:1988108/1‑100 (MQ=255)
                      tcgggATCCGTCAGAAAGTCGCGCTTCCGCCTAACGCTGTGCCTACCTAGACAATTTCTGACGCCTCGTTCGCCGATGCACCGTCAAAATAGCTGGCGCa                                                                             <  2:2769444/97‑1 (MQ=255)
                       cTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAg                                                                            <  2:516635/100‑1 (MQ=255)
                        tGGATCCGTCAAAAAGTCGCAATGCCGCCTTAGGCTGTGATAACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAg                                                                            <  2:2558358/99‑1 (MQ=255)
                          gATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGaaa                                                                         >  1:965340/1‑100 (MQ=255)
                          gATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGaaa                                                                         >  1:652087/1‑100 (MQ=255)
                           aTCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAg                                                                        >  1:2980450/1‑100 (MQ=255)
                              cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCgg                                                                     <  1:1174491/100‑1 (MQ=255)
                              cGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCgg                                                                     >  2:390834/1‑100 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    >  2:3376246/1‑100 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    <  1:3922392/100‑1 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    >  1:2901318/1‑100 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    >  2:4085742/1‑100 (MQ=255)
                               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGt                                                                    >  1:3225522/1‑100 (MQ=255)
                                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTg                                                                   <  2:3197651/100‑1 (MQ=255)
                                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTg                                                                   <  2:2127304/100‑1 (MQ=255)
                                 cAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGc                                                                  >  1:3949096/1‑100 (MQ=255)
                                  aGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCg                                                                 <  2:66423/100‑1 (MQ=255)
                                    aaaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTa                                                               <  1:590296/100‑1 (MQ=255)
                                     aaGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTAc                                                              <  2:2252372/100‑1 (MQ=255)
                                      aGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACt                                                             <  1:1738124/100‑1 (MQ=255)
                                      aGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACt                                                             >  2:1111912/1‑100 (MQ=255)
                                        tCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGa                                                           <  2:1561924/100‑1 (MQ=255)
                                         cGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGaa                                                          >  1:3143281/1‑100 (MQ=255)
                                          gCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAg                                                         >  1:1111214/1‑100 (MQ=255)
                                           cACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAgg                                                        <  1:1868403/100‑1 (MQ=255)
                                           cACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAgg                                                        <  1:2732656/100‑1 (MQ=255)
                                             cTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTa                                                      >  1:2650620/1‑100 (MQ=255)
                                                ccgccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAAc                                                   <  1:3939180/100‑1 (MQ=255)
                                                 cgccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACa                                                  <  1:442790/100‑1 (MQ=255)
                                                 cgccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACa                                                  <  2:2629608/100‑1 (MQ=255)
                                                 atcttGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACa                                                  <  2:628707/96‑1 (MQ=255)
                                                  gccTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAg                                                 >  2:2441320/1‑100 (MQ=255)
                                                    cTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTc                                               <  2:1204622/100‑1 (MQ=255)
                                                     tGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACaagat                                              >  1:628711/1‑96 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            <  1:1208506/100‑1 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            >  1:3367645/1‑100 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            >  2:2590481/1‑100 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAcc                                            <  1:2451024/100‑1 (MQ=255)
                                                       aTGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCAc                                             <  2:1980894/99‑1 (MQ=255)
                                                        tgctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCa                                           >  2:89398/1‑100 (MQ=255)
                                                          ctgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCAtt                                         <  1:1964767/100‑1 (MQ=255)
                                                           tgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTc                                        <  1:2192300/100‑1 (MQ=255)
                                                           tgtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTc                                        >  2:2868539/1‑100 (MQ=255)
                                                            gtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCa                                       <  2:3717423/100‑1 (MQ=255)
                                                              gctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                     >  2:3274336/1‑100 (MQ=255)
                                                                tgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCtt                                   <  2:1145332/100‑1 (MQ=255)
                                                                tgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCtt                                   >  1:326190/1‑100 (MQ=255)
                                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCttt                                  >  1:991077/1‑100 (MQ=255)
                                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCttt                                  >  1:4124665/1‑100 (MQ=255)
                                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTAAAAAGTGCTGGCGCAGAAAGCGGTGCGAACTGAAGGTAAACAGCCACCATTCAGCttt                                  >  2:396986/1‑100 (MQ=255)
                                                                   ccGTGACACTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGt                                <  2:3825426/100‑1 (MQ=255)
                                                                    cGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGtt                               <  2:3119080/100‑1 (MQ=255)
                                                                     gTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGCAGGTAAACAGTCCCCATTCAGCTTTGtt                               >  2:1979003/1‑99 (MQ=255)
                                                                        acacTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                           <  1:2950455/100‑1 (MQ=255)
                                                                        acacTTTCTGACGCGTCGTTAGCCAGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                           >  2:307209/1‑100 (MQ=255)
                                                                        acacTTTCTGACGCATCTTTAGTCGGTGCACTGTCAAAAGTTCTGGCGCAGAAATCGGTGTGTACTGAATGTAATCAGTTACCATTCAGCTTTGTTCTg                            <  2:1434211/99‑1 (MQ=255)
                                                                         cacTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTc                          >  1:829550/1‑100 (MQ=255)
                                                                         cacTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTc                          >  2:3319533/1‑100 (MQ=255)
                                                                          tcTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCa                         <  2:1341147/99‑1 (MQ=255)
                                                                           cTTTCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCAt                        >  1:1170628/1‑100 (MQ=255)
                                                                           cTTTCTGACGCGTCGTTAGCCGATGCACCGTGAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCAt                        <  2:3834689/100‑1 (MQ=255)
                                                                            tttCTGACGCGTCGTTAGCCGATGCAGCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTAAGCTTTGTTCTGTCAtt                       <  2:3490579/100‑1 (MQ=255)
                                                                             ttCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCaaga                      >  1:1341155/1‑97 (MQ=255)
                                                                              tCTGACGCGTCGTTAGCCGGTGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTa                     >  1:2415258/1‑100 (MQ=255)
                                                                                tGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAc                   >  2:1078806/1‑100 (MQ=255)
                                                                                 gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAcc                  >  2:1706910/1‑100 (MQ=255)
                                                                                 gACGCGTCGTTAGCCGATGAACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAcc                  >  1:1909162/1‑100 (MQ=255)
                                                                                   cgcgTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                >  1:3158188/1‑100 (MQ=255)
                                                                                   cgcgTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                >  2:3205172/1‑100 (MQ=255)
                                                                                   cgcgTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                <  2:1928402/100‑1 (MQ=255)
                                                                                     cgtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc              <  2:948602/100‑1 (MQ=255)
                                                                                     cgtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGc              >  2:2089614/1‑100 (MQ=255)
                                                                                      gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCa             <  2:2291063/100‑1 (MQ=255)
                                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAg            <  2:1267080/100‑1 (MQ=255)
                                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAg            <  1:1111917/100‑1 (MQ=255)
                                                                                        cgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGa           >  1:726457/1‑100 (MQ=255)
                                                                                         gtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGATTTGTTCTGTCATTTAACCCGGCAGAt          >  1:212399/1‑100 (MQ=255)
                                                                                           tAGCCGATGCACCGTCAAAAGTGGTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATg         <  2:3528177/99‑1 (MQ=255)
                                                                                           tAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGt        <  1:71327/100‑1 (MQ=255)
                                                                                           tAGCCGATGAACCGTCAAAAGTGCTGGCGCCGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCAATCAGATTTGTTCTGTCATTTAACCCGGCAGATg         >  2:3965881/1‑99 (MQ=255)
                                                                                            aGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGtt       >  2:3295393/1‑100 (MQ=255)
                                                                                            aGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGtt       >  1:3578120/1‑100 (MQ=255)
                                                                                             gCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTc      >  2:4124803/1‑100 (MQ=255)
                                                                                             gCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTAAGCTTTGTTCTGTCATTTAACCCGGAAgctttt       >  1:3776484/1‑94 (MQ=255)
                                                                                                 |                                                                                                   
CAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCC  >  NC_000913/475517‑475713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: