Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,134,852 A→G 74.8% intergenic (+29/+20) yghT → / ← pitB putative ATP‑binding protein/phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,134,8520AG74.8% 337.2 / 105.9 190intergenic (+29/+20)yghT/pitBputative ATP‑binding protein/phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base G (77/64);  total (97/93)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 191 total aligned reads displayed.

TGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134756‑3134946
                                                                                                |                                                                                              
 ggAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCgg                                                                                            >  1:2578739/1‑100 (MQ=255)
    aCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCa                                                                                         >  1:1087414/1‑100 (MQ=255)
     cAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAt                                                                                        >  1:131394/1‑100 (MQ=255)
      aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAtt                                                                                       <  1:2376738/100‑1 (MQ=255)
      aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAtt                                                                                       >  2:3541328/1‑100 (MQ=255)
       ggTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCAttt                                                                                      >  2:1172527/1‑100 (MQ=255)
         tgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTc                                                                                    <  1:2056732/100‑1 (MQ=255)
          gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGGAATGCCCGATCGCCAGGGCCGGGCATTTTCa                                                                                   <  2:1144082/100‑1 (MQ=255)
          gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCa                                                                                   >  2:2596791/1‑100 (MQ=255)
            tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAgg                                                                                 >  2:2997442/1‑100 (MQ=255)
            tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAgg                                                                                 >  2:201928/1‑100 (MQ=255)
            tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAgg                                                                                 <  1:1357434/100‑1 (MQ=255)
             ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGa                                                                                >  1:2602842/1‑100 (MQ=255)
             ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                                >  2:1371340/1‑100 (MQ=255)
             ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGCACCGGGCAttttac                                                                                   >  2:2288239/1‑95 (MQ=255)
                tgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTGTCAGTAGTCAAAAATGGAAATGCCCGAGCGCCAGGACCGGGCGTTTTCAGTAAgg                                                                             >  2:4175020/1‑100 (MQ=255)
                tgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGTAAATGACCGATCGCCAGGACCGGGCATTTTCAgg                                                                                 >  2:1482136/1‑96 (MQ=255)
                tgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAgg                                                                             <  1:3635836/100‑1 (MQ=255)
                 gATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGt                                                                            >  1:89714/1‑100 (MQ=255)
                  aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGtt                                                                           >  2:1454838/1‑100 (MQ=255)
                    gCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTaa                                                                         >  1:3359300/1‑100 (MQ=255)
                    gCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         <  1:2416234/100‑1 (MQ=255)
                      aGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGGTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAAt                                                                       <  2:3696030/100‑1 (MQ=255)
                       gAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATc                                                                      <  2:2590258/100‑1 (MQ=255)
                       gAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATc                                                                      <  1:2975910/100‑1 (MQ=255)
                       gAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATc                                                                      >  1:3371501/1‑100 (MQ=255)
                       gAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTGTCAGGAAGGTTaaa                                                                        >  2:3098016/1‑98 (MQ=255)
                         aaaaGCGATTACGGATTTTATGACCGCGCGTGGATATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGAATTTTCAGCAAGGTTAAATCaa                                                                    >  2:1530702/1‑100 (MQ=255)
                          aaaGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAAc                                                                   <  1:730343/100‑1 (MQ=255)
                           aaGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACt                                                                  <  1:2030775/100‑1 (MQ=255)
                             gCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                 <  2:4070661/99‑1 (MQ=255)
                             gCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGc                                                                <  1:2581330/100‑1 (MQ=255)
                               aagtACGGATTTTATGACAGAGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGAGCATTTTCAAGAAGGTTAAATCAACTGCaa                                                              >  2:1609987/4‑100 (MQ=255)
                                aTTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAAt                                                             <  2:1348459/100‑1 (MQ=255)
                                aTTACGGATTTTATGACCGCGCGTGGCTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATCTTCAGGAATGTTAAATCAACTGCAAt                                                             >  2:342660/1‑100 (MQ=255)
                                  tACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                           <  1:1015640/100‑1 (MQ=255)
                                   aCGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                           <  2:3143341/99‑1 (MQ=255)
                                   aCGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCt                                                          >  1:2637703/1‑100 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGc                                                           <  2:1441268/98‑1 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                         >  2:1857368/1‑100 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                         >  1:2349219/1‑100 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                         <  1:3926315/100‑1 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                         <  1:2796996/100‑1 (MQ=255)
                                    cGGATTTTATGACCGCGCGTGGTAATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                         >  2:1222006/1‑100 (MQ=255)
                                       aTTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATcc                                                      <  1:153893/100‑1 (MQ=255)
                                       aTTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATcc                                                      <  1:370514/100‑1 (MQ=255)
                                       aTTTTATGAACGCGCGGGGTTAACACTATTCAAAAATGGAAAGGCCCCATCGCCAGAGCCCGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATcc                                                      <  2:2560682/100‑1 (MQ=255)
                                        ttttATGACCGCGCGTGGTTCTCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATcc                                                      <  2:786786/99‑1 (MQ=255)
                                         tttATGACCGCGCGTGGTTATCACTAGTCAAAAATGGGAATGCCTTATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAg                                                    <  2:2641634/100‑1 (MQ=255)
                                         tttATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAg                                                    <  2:3989520/100‑1 (MQ=255)
                                          ttATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGt                                                   >  1:2623295/1‑100 (MQ=255)
                                          ttATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCAGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGt                                                   <  1:3837940/100‑1 (MQ=255)
                                           tATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCCGTa                                                  <  1:345520/100‑1 (MQ=255)
                                           tATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                  >  1:1701975/1‑100 (MQ=255)
                                           tATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                  <  1:1495956/100‑1 (MQ=255)
                                             tGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACa                                                >  1:2217244/1‑100 (MQ=255)
                                             tGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACa                                                >  1:2098009/1‑100 (MQ=255)
                                              gACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                               <  2:1271343/100‑1 (MQ=255)
                                               aCCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                               <  1:2110804/99‑1 (MQ=255)
                                               aCCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGc                                              >  2:1233805/1‑100 (MQ=255)
                                                 cgcgcgTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGccc                                            <  2:3908980/100‑1 (MQ=255)
                                                      gTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                         <  1:3998804/98‑1 (MQ=255)
                                                        ggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaa                                     <  1:1172532/100‑1 (MQ=255)
                                                             tCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAgaaagaaa                                >  2:1066688/1‑100 (MQ=255)
                                                              cACTAGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGaaaa                               <  1:3136258/100‑1 (MQ=255)
                                                                  aGTCAAAAATGGAAATTCCTGATCGCCAGGACCTGGCATTTTCAGAAAGGTTAAATCAACTGCAATGCTACCCAGTACAGCCCACCAGAAAGaaaaa                              <  2:2473016/97‑1 (MQ=255)
                                                                  aGTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTg                           <  1:4088486/100‑1 (MQ=255)
                                                                   gccAAAAATGGAAATGCCCGAAAGCCAGGGCCGGACATTTTCAGGAAAGATAAATAAACTTCAATCCTATCAAATACAGCGCACAACAAAGATAAATTgc                          >  2:3898754/3‑100 (MQ=255)
                                                                   gTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgc                          >  1:3987739/1‑100 (MQ=255)
                                                                   gTCAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgc                          >  2:3060747/1‑100 (MQ=255)
                                                                    tCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                         <  2:914586/100‑1 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTCTCCAGTACAGCCCACCAGAAAGAAAAATTgccg                        >  1:1556233/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                        >  1:2037404/1‑100 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                        <  1:615519/100‑1 (MQ=255)
                                                                     cAAAAATGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                        >  2:2854821/1‑100 (MQ=255)
                                                                      aaaaaTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgc                       >  2:2814380/1‑100 (MQ=255)
                                                                       aaaaTGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgcc                      <  2:515886/100‑1 (MQ=255)
                                                                       aaaaTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgcc                      >  2:284107/1‑100 (MQ=255)
                                                                        acaTGGAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGccgccg                     <  2:2520993/98‑1 (MQ=255)
                                                                           tGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                  >  2:3946783/1‑100 (MQ=255)
                                                                            ggAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                 >  1:2914565/1‑100 (MQ=255)
                                                                            ggAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                 <  2:2887905/100‑1 (MQ=255)
                                                                             gAAATGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaa                >  1:1011695/1‑100 (MQ=255)
                                                                              aaaTGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaag               >  1:970537/1‑100 (MQ=255)
                                                                              aaaTGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCACTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaag               <  2:2217230/100‑1 (MQ=255)
                                                                                 tGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagtaa            >  1:2515275/1‑100 (MQ=255)
                                                                                 tGCCCGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagtaa            <  1:3999233/100‑1 (MQ=255)
                                                                                  gCCCTATCGCCAGTGCCGGGCATTTTCAGGAAGGTGAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaactcaa           >  2:603296/1‑95 (MQ=255)
                                                                                    ccGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa         <  1:1193235/100‑1 (MQ=255)
                                                                                    ccGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa         >  1:2852223/1‑100 (MQ=255)
                                                                                    ccGATCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa         >  1:464576/1‑100 (MQ=255)
                                                                                       aTCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAccc      >  1:3241124/1‑100 (MQ=255)
                                                                                        tCGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa     <  2:3757750/100‑1 (MQ=255)
                                                                                         cGCCAGGGCCGGGCATTTTCAGGCAGGTTAAATCAACTGCAATGCTATACAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc    >  2:924790/1‑100 (MQ=255)
                                                                                         cGCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc    >  2:2086235/1‑100 (MQ=255)
                                                                                         cGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATAccta     <  1:2814396/99‑3 (MQ=255)
                                                                                         cGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc    <  2:663545/100‑1 (MQ=255)
                                                                                          gCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACg   <  1:1857378/100‑1 (MQ=255)
                                                                                          gCCAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACg   >  1:3103938/1‑100 (MQ=255)
                                                                                           ccAGGGCCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc  >  1:1874901/1‑100 (MQ=255)
                                                                                                |                                                                                              
TGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134756‑3134946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: