Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,195,102 G→A 76.6% intergenic (+277/‑39) ibsD → / → ibsE toxic membrane protein/toxic membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,195,1020GA76.6% 279.7 / 90.4 169intergenic (+277/‑39)ibsD/ibsEtoxic membrane protein/toxic membrane protein
Reads supporting (aligned to +/- strand):  ref base G (14/25);  new base A (48/79);  total (63/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 169 total aligned reads displayed.

GACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTA  >  NC_000913/3195005‑3195199
                                                                                                 |                                                                                                  
gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAAcc                                                                                                  <  1:3905873/100‑1 (MQ=255)
gACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCAGCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAAcc                                                                                                  <  2:2137888/100‑1 (MQ=255)
   cacTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCt                                                                                              >  2:2009884/3‑100 (MQ=255)
    gCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTc                                                                                             >  1:108934/1‑100 (MQ=255)
    gCTCGGGGAAGGCAAAATTGCCTCAGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTc                                                                                             <  2:3726890/100‑1 (MQ=255)
      tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTccc                                                                                           <  1:3921952/100‑1 (MQ=255)
      tCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTccc                                                                                           <  1:1519630/100‑1 (MQ=255)
       cGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCg                                                                                          <  1:1689946/100‑1 (MQ=255)
        ggggCAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACACTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCg                                                                                          <  2:1384619/99‑1 (MQ=255)
        ggggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCga                                                                                         <  2:3869574/100‑1 (MQ=255)
         gggAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCgaa                                                                                        <  1:3016283/100‑1 (MQ=255)
           gAAGGCAAAATTGCCTCTGGGAAAGCATTTCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCgaaga                                                                                      >  2:1842881/1‑100 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAgg                                                                                   <  2:3052891/100‑1 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAgg                                                                                   >  1:3702250/1‑100 (MQ=255)
              ggCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAgg                                                                                   >  2:1717548/1‑100 (MQ=255)
               gCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAggg                                                                                  <  2:2726929/100‑1 (MQ=255)
                 aaaaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGc                                                                                <  1:1466283/100‑1 (MQ=255)
                   aaTTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCt                                                                              <  1:1298312/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGGTAATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTg                                                                            <  2:1388934/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGTAGAAGGGGCCTTg                                                                            <  1:2832774/100‑1 (MQ=255)
                     ttGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTg                                                                            <  1:1169296/100‑1 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtat                                                                         <  2:793025/100‑1 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGtat                                                                         >  1:2402758/1‑100 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGtat                                                                         >  1:214709/1‑100 (MQ=255)
                        ccTCTGGGAAAGCATTGCGCGGGGTCCGGCGATCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAACCCCCTCCCGAAGAAGGGGCCTTGtat                                                                         >  2:3818899/1‑100 (MQ=255)
                         ctctGGGAAAGCGTTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtata                                                                        >  1:2738750/1‑100 (MQ=255)
                         ctctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGtata                                                                        >  2:3402655/1‑100 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                                      >  2:3069606/1‑100 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  1:2096531/100‑1 (MQ=255)
                           ctGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  2:1644279/100‑1 (MQ=255)
                           ctGGGAAAGCAGTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                                      <  2:1924462/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  1:1697359/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:2440769/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:668434/100‑1 (MQ=255)
                            tGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCCGGCGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                     <  2:1026413/100‑1 (MQ=255)
                              ggAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaa                                                                   >  1:2609641/1‑100 (MQ=255)
                                  aGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAggg                                                               <  2:1288008/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:1856392/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  2:3697285/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:729798/100‑1 (MQ=255)
                                   gCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              >  2:4041240/1‑100 (MQ=255)
                                   gCATTGCGCGGGGTCCGGAGCGCAGCAACAATCGGGGGGCAGCAAGGCGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                              <  1:2278751/100‑1 (MQ=21)
                                      ttGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                            <  1:3108583/99‑1 (MQ=255)
                                      ttGCGCGGGGTCCGGCGCTCATCAACAAGCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                            <  1:123289/99‑1 (MQ=255)
                                       tGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                          <  1:2044506/100‑1 (MQ=255)
                                        gcgcGGGGTCCGGCGCTCATCAAAAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatga                                                         >  2:2324125/1‑100 (MQ=255)
                                        gcgcGGGGGCGGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                          <  2:2462009/99‑1 (MQ=255)
                                         cgcgGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatgat                                                        <  2:1493738/100‑1 (MQ=255)
                                           cgGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTAtgatga                                                      <  2:1734138/100‑1 (MQ=255)
                                              ggTCCGGCGCTGATCAACAATCTGGGAGTAGCAAGGGGGTGCAAAGGGAACGCCCCTCCCGAAGCCGGGGTCTGGTTTAAGGAAAGGGTTatgatg                                                       <  2:2426213/96‑1 (MQ=12)
                                              ggTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGc                                                   <  2:616672/100‑1 (MQ=255)
                                               gTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt                                                  >  2:3417710/1‑100 (MQ=255)
                                               gTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCt                                                  >  2:3782754/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCGAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                >  1:3729996/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                >  1:3592746/1‑100 (MQ=255)
                                                 ccGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCg                                                <  2:108932/100‑1 (MQ=255)
                                                   ggCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                              <  1:341178/100‑1 (MQ=255)
                                                    gcgcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtca                                             <  1:2898129/100‑1 (MQ=255)
                                                      gcccttcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcatc                                           <  1:3805254/95‑1 (MQ=255)
                                                        tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat                                         <  2:3847561/100‑1 (MQ=255)
                                                        tcatcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTcatcat                                         >  2:4154006/1‑100 (MQ=255)
                                                            caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                     >  1:809283/1‑100 (MQ=255)
                                                            caacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                     >  1:1130222/1‑100 (MQ=255)
                                                             aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                                    >  1:1393006/1‑100 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGGTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                                   <  1:943118/100‑1 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGTGGCCTTTTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                       <  2:3983324/96‑1 (MQ=255)
                                                              acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                                   <  1:100954/100‑1 (MQ=255)
                                                               caaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                  >  2:1937677/1‑100 (MQ=255)
                                                               caaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                  >  2:3259084/1‑100 (MQ=255)
                                                                aaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                                 <  1:86631/100‑1 (MQ=255)
                                                                 aTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAACCCCGCCCGAAGAAGGGGCCTTGTATAAGAAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgt                                >  2:3875534/1‑100 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             <  1:1046460/100‑1 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             >  2:2286974/1‑100 (MQ=255)
                                                                    ggggggCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgt                              >  1:3905115/1‑99 (MQ=255)
                                                                     gggggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                            <  1:128309/100‑1 (MQ=255)
                                                                      ggggCAGCAAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                           >  1:3743160/1‑100 (MQ=255)
                                                                         gcagcaAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTAc                        <  1:126275/100‑1 (MQ=255)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGTTCCTTGTATAAAGAAAGTGTTATGATGAAGCTCGTCATCATACTGgttgtgtt                             <  1:1348945/94‑1 (MQ=17)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                       <  2:3369487/100‑1 (MQ=255)
                                                                          cagcaAGGGGCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                       <  1:3576308/100‑1 (MQ=255)
                                                                              atggggGTGAGAAGGGAAAGCGCCTCCCTAAGAAGGGGCGTGGTATAAGGAAAGGGTTATTATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                   <  2:3292153/98‑1 (MQ=17)
                                                                              agggggGTGAAACGGGAAAGCCCCTTCCGGAGGAAGGGGCCTTACATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTg                      <  2:1950380/96‑1 (MQ=11)
                                                                                 gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGGAAGGGTTATGATGAAGGTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                 >  2:137632/1‑99 (MQ=255)
                                                                                 gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGt                <  2:2989692/100‑1 (MQ=255)
                                                                                 gggCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGGATAAGGAAAAGGTTACGATGAAGCTCGTCAGCATACTGGCTGTGTTGTTACTGTTAAg                 >  2:1563500/1‑99 (MQ=17)
                                                                                  ggCTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt               <  2:319123/100‑1 (MQ=255)
                                                                                    cTGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc             >  1:883955/1‑100 (MQ=255)
                                                                                     tGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTcc            <  1:2981984/100‑1 (MQ=255)
                                                                                     tGAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTcc            <  2:3058990/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAGCCCCTCCCGAAGAAGGGGTCTTGTATAAGAAAAGGGATATGATGAATGTCGTCATCATACTGATTGTGTTGTTACTGTTAAGtttac            >  2:301213/1‑97 (MQ=17)
                                                                                      gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           >  2:3771908/1‑100 (MQ=255)
                                                                                      gAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           <  2:2011168/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           <  2:2059659/100‑1 (MQ=255)
                                                                                      gAAACGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc           >  1:1503016/1‑100 (MQ=255)
                                                                                        aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa         >  1:436819/1‑100 (MQ=255)
                                                                                         aCGGGAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGAc        <  2:1511972/100‑1 (MQ=255)
                                                                                           gggAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt      <  1:1334470/100‑1 (MQ=255)
                                                                                            ggAAAACCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTa     <  2:438358/100‑1 (MQ=255)
                                                                                              aaaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt   <  1:1427005/100‑1 (MQ=255)
                                                                                              aaaGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt   <  1:569625/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GACCGCTCGGGGAAGGCAAAATTGCCTCTGGGAAAGCATTGCGCGGGGTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTA  >  NC_000913/3195005‑3195199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: