Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,968 A→G 74.7% G812G (GGT→GGC acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,9680AG74.7% 206.8 / 72.0 122G812G (GGT→GGCacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (16/15);  new base G (34/57);  total (50/72)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

Only 100 of 123 total aligned reads displayed.

CATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGGCAGCATACGG  >  NC_000913/481872‑482067
                                                                                                |                                                                                                   
cATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCg                                                                                                  >  1:681522/1‑100 (MQ=255)
 aTCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGACGAACCGt                                                                                                 >  1:2413002/1‑100 (MQ=255)
 aTCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGCATGGCAGGCCGTTGTAACGTTCTAGACGCGGCGAGCCGt                                                                                                 >  2:1696929/1‑100 (MQ=255)
  tCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTa                                                                                                >  2:365544/1‑100 (MQ=255)
   cAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTAc                                                                                               >  2:3192619/1‑100 (MQ=255)
   cAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTAc                                                                                               <  2:598688/100‑1 (MQ=255)
   cAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTAc                                                                                               <  2:432801/100‑1 (MQ=255)
     gCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTc                                                                                             >  2:2127137/1‑100 (MQ=255)
     gCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTc                                                                                             <  2:3543087/100‑1 (MQ=255)
     gCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTc                                                                                             <  2:1564743/100‑1 (MQ=255)
     gCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTc                                                                                             <  2:1951421/100‑1 (MQ=255)
      cTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTcc                                                                                            <  2:4104851/100‑1 (MQ=255)
        ccATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAGCGTTCCAGACGCGGCGAGCCGTACTCCCa                                                                                          >  2:2577836/1‑100 (MQ=255)
           ttGCTGCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACg                                                                                       <  2:4019981/100‑1 (MQ=255)
             gCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACgag                                                                                     >  1:1002532/1‑100 (MQ=255)
              cTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACgaga                                                                                    <  1:2697820/100‑1 (MQ=255)
               ttCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGagaa                                                                                   >  2:4158252/1‑100 (MQ=255)
                tCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGagaag                                                                                  <  2:3999419/100‑1 (MQ=255)
                tCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGagaag                                                                                  <  2:517148/100‑1 (MQ=255)
                tCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGagaag                                                                                  <  2:1423340/100‑1 (MQ=255)
                 cACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGagaaga                                                                                 >  1:874094/1‑100 (MQ=255)
                  aCCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGACTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGagaaga                                                                                 >  2:3387822/1‑99 (MQ=255)
                   ccGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAgagg                                                                               >  1:1548949/1‑100 (MQ=255)
                   ccGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAgagg                                                                               >  1:2352084/1‑100 (MQ=255)
                    cGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAgagga                                                                              <  2:3486441/100‑1 (MQ=255)
                     ggTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAgaggag                                                                             >  1:3097337/1‑100 (MQ=255)
                     ggTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAgaggag                                                                             >  2:523374/1‑100 (MQ=255)
                         cTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACg                                                                         >  2:2603899/1‑100 (MQ=255)
                         cTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACg                                                                         <  2:3201429/100‑1 (MQ=255)
                         cTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACg                                                                         >  2:2603986/1‑100 (MQ=255)
                         cTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACg                                                                         <  2:337910/100‑1 (MQ=255)
                         cTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGCCGAGCCGTCCTCCCAACGAGAAGAGGAGAACg                                                                         >  1:963162/1‑100 (MQ=255)
                          ttttACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGc                                                                        <  2:2116216/100‑1 (MQ=255)
                          ttttACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGc                                                                        >  1:2113228/1‑100 (MQ=255)
                          ttttACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGc                                                                        <  1:593560/100‑1 (MQ=255)
                           tttACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGcc                                                                       >  1:3774904/1‑100 (MQ=255)
                             tACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCga                                                                     <  1:1184441/100‑1 (MQ=255)
                              aCCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCgag                                                                    >  1:1062188/1‑100 (MQ=255)
                                 cGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAAt                                                                 <  1:639456/100‑1 (MQ=255)
                                  ggTGCCGCCTGGCCTAAGCTTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATg                                                                <  2:2825449/100‑1 (MQ=255)
                                  ggTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATg                                                                <  2:384484/100‑1 (MQ=255)
                                  ggTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATg                                                                <  2:1941790/100‑1 (MQ=255)
                                   gTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATgg                                                               <  1:1907604/100‑1 (MQ=255)
                                    tGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGc                                                              <  1:1278046/100‑1 (MQ=255)
                                    tGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGc                                                              <  1:538482/100‑1 (MQ=255)
                                       cgccTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGCGAAGAGGAGAACGCCGAGAATGGCAcc                                                           >  2:914972/1‑100 (MQ=255)
                                          cTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATc                                                        <  1:2371003/100‑1 (MQ=255)
                                            ggCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTg                                                      <  1:2127148/100‑1 (MQ=255)
                                            ggCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTg                                                      <  1:2324146/100‑1 (MQ=255)
                                              ccTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGAc                                                    <  1:2154008/100‑1 (MQ=255)
                                               cTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGAcc                                                   <  2:1614634/100‑1 (MQ=255)
                                                tAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCa                                                  >  1:3873667/1‑100 (MQ=255)
                                                tAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCa                                                  <  1:3192639/100‑1 (MQ=255)
                                                  aGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATc                                                <  1:3621953/100‑1 (MQ=255)
                                                   gATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATca                                               <  1:2835851/100‑1 (MQ=255)
                                                   gATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAACAGGAGACCGCCGAGAATGGCACCATCTAACCAt                                                 >  2:511170/1‑98 (MQ=37)
                                                    aTTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATcag                                              <  1:3185496/100‑1 (MQ=255)
                                                    aTTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCACCTGACCATca                                               >  1:1689695/1‑99 (MQ=255)
                                                     tttCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATcagc                                             <  2:1929274/100‑1 (MQ=255)
                                                     tttCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATcagc                                             <  2:1603535/100‑1 (MQ=255)
                                                      ttCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTTCTCCCAACGAGACTAGGAGAACGCCGAGAATGGCACCATCTAACCa                                                  >  2:2823608/1‑94 (MQ=38)
                                                       tCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATcagcag                                           <  2:1484120/100‑1 (MQ=255)
                                                        ccATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCGGAGAATGGCACCATCTGACCATCagcagc                                          >  2:2240520/1‑100 (MQ=255)
                                                        ccATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCagcagc                                          <  2:1908574/100‑1 (MQ=255)
                                                         cATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAgcagca                                         <  2:1128506/100‑1 (MQ=255)
                                                         cATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAgcagca                                         >  1:1964118/1‑100 (MQ=255)
                                                          atggatggCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAgcagca                                         <  1:1814023/99‑1 (MQ=255)
                                                          atggatggCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCAc                                        <  1:1673375/100‑1 (MQ=255)
                                                                ggCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACAt                                  <  2:3638347/100‑1 (MQ=255)
                                                                ggCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACAt                                  >  1:2529051/1‑100 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 >  2:202070/1‑100 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 <  1:1403386/100‑1 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 >  1:2065368/1‑100 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 >  1:2065807/1‑100 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 >  1:3883621/1‑100 (MQ=255)
                                                                 gCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATa                                 >  1:1540675/1‑100 (MQ=255)
                                                                  cAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGCACATAc                                >  2:2639908/1‑100 (MQ=255)
                                                                   aGGCGGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  1:157789/100‑1 (MQ=255)
                                                                   aGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  1:4098548/100‑1 (MQ=255)
                                                                   aGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  1:3586664/100‑1 (MQ=255)
                                                                   aGGCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCAAAGAATGGCATCATCTGGCTGTGAGCAGCACGaagaggca                               <  2:796403/100‑7 (MQ=255)
                                                                   aGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  1:2358466/100‑1 (MQ=255)
                                                                    ggCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  2:3526216/99‑1 (MQ=255)
                                                                    ggCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATAcc                               <  1:2014682/99‑1 (MQ=255)
                                                                    ggCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAACGGCACCATCTACCCATCATCAGCACGAACATAcc                               >  2:821969/1‑99 (MQ=255)
                                                                     gCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAg                             <  2:1342071/100‑1 (MQ=255)
                                                                     gCCGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAg                             >  1:2687615/1‑100 (MQ=255)
                                                                      ccGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGt                            <  1:2726558/100‑1 (MQ=255)
                                                                      ccGTTGTAACGTTCCAGACGCGGCGAGCCGTACTCCCAACGAACAGAGGAGCCAGCCGAGCCTGGCACCATCTGCCCATCAGCAGCACGAACATACAAg                             >  2:3421403/1‑99 (MQ=255)
                                                                            tAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACAAGGAGCAGCACGAACATACCAGt                            >  2:3525226/1‑94 (MQ=255)
                                                                             aaCGTTCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGat                     <  2:36133/100‑1 (MQ=255)
                                                                               cGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGatat                   <  2:1986137/100‑1 (MQ=255)
                                                                                  tCCAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGatataa                 >  2:3418783/1‑97 (MQ=255)
                                                                                    cAGACGCGGCGAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATcc              >  1:2398291/1‑100 (MQ=255)
                                                                                       aCGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGgc           <  1:458746/100‑1 (MQ=255)
                                                                                          cggcgAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGgcagc        <  1:2735540/100‑1 (MQ=255)
                                                                                          cggcgAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGgcagc        <  2:804286/100‑1 (MQ=255)
                                                                                           ggcgAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGgcagca       <  2:3347289/100‑1 (MQ=255)
                                                                                           ggcgAGCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCAGgcagca       >  2:1245743/1‑100 (MQ=255)
                                                                                                aCCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGGCAGCATACgg  >  1:2491636/1‑100 (MQ=255)
                                                                                                |                                                                                                   
CATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGACGCGGCGAACCGTACTCCCAACGAGAAGAGGAGAACGCCGAGAATGGCACCATCTGACCATCAGCAGCACGAACATACCAGTCGCCGATATCATCCGGCAGCATACGG  >  NC_000913/481872‑482067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: