BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,452,057	A→T	100%	I77N (ATC→AAC)	rplD ←	50S ribosomal subunit protein L4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,452,057	0	A	T	100.0%	634.8 / ‑6.7	189	I77N (ATC→AAC)	rplD	50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand): ref base A (0/1); major base T (104/83); minor base C (1/0); total (105/84)

Only 100 of 189 total aligned reads displayed.

CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGA  >  NC_000913/3451960‑3452152

 atgATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTc                                                                                               <  2:394935/97‑1 (MQ=255)

 aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCg                                                                                              <  1:1281824/100‑1 (MQ=255)

  ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                             >  1:3728885/1‑100 (MQ=255)

  ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                             >  1:3185906/1‑100 (MQ=255)

   gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCggg                                                                                            <  1:2619440/100‑1 (MQ=255)

    aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGc                                                                                           <  1:608796/100‑1 (MQ=255)

       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                        >  1:3460905/1‑100 (MQ=255)

       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                        >  2:1346542/1‑100 (MQ=255)

       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                        >  1:855134/1‑100 (MQ=255)

       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                        >  2:3448699/1‑100 (MQ=255)

       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                        >  2:2131718/1‑100 (MQ=255)

        ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt                                                                                       <  1:2122470/100‑1 (MQ=255)

        tttgCAGCGCGGCGGGGGACATCTTCGTGTTAGCTTTTGGGCGGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCGCCAGAACGCCAGTTCGGGct                                                                                          <  2:3684384/97‑1 (MQ=255)

         tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTGGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTg                                                                                      >  2:2229568/1‑100 (MQ=255)

          ttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGa                                                                                     <  2:3743047/100‑1 (MQ=255)

           tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                    <  2:1925983/100‑1 (MQ=255)

           tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                    >  1:345979/1‑100 (MQ=255)

           tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                    >  2:3576239/1‑100 (MQ=255)

            cAGCGCGCCGCGGTACCTCTTCTTGTTAACTTTTTGCCTGTGCTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                    <  2:3477623/99‑1 (MQ=255)

              gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                 >  2:4107766/1‑100 (MQ=255)

                gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAc                                                                               <  2:3970104/100‑1 (MQ=255)

                 cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCCCCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                              >  2:64539/1‑100 (MQ=255)

                 cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                              <  2:2468785/100‑1 (MQ=255)

                 cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                              <  1:1848840/100‑1 (MQ=255)

                  gccgcGGTACATCTTCTTGTTAACTTTTTGCCTGTGGCACTGCGGACGAGCAGCAAAGGTAACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCa                                                                             <  1:3323710/100‑1 (MQ=255)

                   ccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAg                                                                            >  2:1052874/1‑100 (MQ=255)

                       ggTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCATCAGAAGGCCAGTTCGGGCTCTTGATAGAACCAGAAcg                                                                        >  2:2807503/1‑100 (MQ=255)

                        gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgc                                                                       <  1:2182568/100‑1 (MQ=255)

                        gTACATCTTCTTGTTAACGTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgc                                                                       <  2:1709208/100‑1 (MQ=255)

                         tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGCTCGGGCTCTTGATAGAACCAGAAcgcg                                                                      >  2:148590/1‑100 (MQ=255)

                           cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACgcgc                                                                     >  2:414516/1‑99 (MQ=255)

                            aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                   >  2:4014195/1‑100 (MQ=255)

                             tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACg                                                                  >  1:3998435/1‑100 (MQ=255)

                             tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACg                                                                  <  1:2724185/100‑1 (MQ=255)

                              cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg                                                                 >  1:793765/1‑100 (MQ=255)

                                tcttGTTAACTTTTTTACTTTTGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGTACGCCAGTTCGGACTCTTGATATCACCATAACGCGCACGAcc                                                               >  2:1949082/1‑100 (MQ=255)

                                    gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                           >  1:910841/1‑100 (MQ=255)

                                    gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                           >  1:1166844/1‑100 (MQ=255)

                                     ttAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                          >  2:711680/1‑100 (MQ=255)

                                        aCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCtt                                                       >  1:543143/1‑100 (MQ=255)

                                         cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                      >  1:1621014/1‑100 (MQ=255)

                                           ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCATACCGCGCACGGCCGGTGCCTTTCt                                                    <  2:3568046/100‑1 (MQ=255)

                                           ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt                                                    >  2:1223164/1‑100 (MQ=255)

                                             ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGCACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTgg                                                  <  2:1276519/100‑1 (MQ=255)

                                             ttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTgg                                                  <  1:2264131/100‑1 (MQ=255)

                                               gACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                >  1:3517951/1‑100 (MQ=255)

                                               gACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                >  1:178494/1‑100 (MQ=255)

                                                 cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTTCCTTTCTGGCGcc                                              <  2:2573824/100‑1 (MQ=255)

                                                 cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                              >  1:1927956/1‑100 (MQ=255)

                                                      gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                           >  2:298279/1‑98 (MQ=255)

                                                       tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtt                                        <  2:2623302/100‑1 (MQ=255)

                                                        ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGACCCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                       <  1:3969485/100‑1 (MQ=255)

                                                         cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                      <  1:3477598/100‑1 (MQ=255)

                                                         cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                       >  1:130716/1‑99 (MQ=255)

                                                           gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt                                    <  1:4095340/100‑1 (MQ=255)

                                                           gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt                                    >  2:1595134/1‑100 (MQ=255)

                                                           gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt                                    >  2:31080/1‑100 (MQ=255)

                                                           gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGACAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCGCGGtgtttt                                    >  2:1382243/1‑100 (MQ=255)

                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTGTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACAGGATTTTAcc                                 >  2:1438155/1‑98 (MQ=255)

                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCGGGTGCCTTTCTGGCGCCACGGCTTTTTACCg                                >  1:3099244/1‑99 (MQ=255)

                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                               >  1:2743733/1‑100 (MQ=255)

                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                               >  2:3650530/1‑100 (MQ=255)

                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                               >  1:1237248/1‑100 (MQ=255)

                                                                 gAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                              <  1:2797456/100‑1 (MQ=255)

                                                                  agcagcAAAGGTCACGCCGCCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                              <  2:1500783/99‑1 (MQ=255)

                                                                  agcagcAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGaa                             >  1:1633153/1‑100 (MQ=255)

                                                                   gcagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc                            <  1:3251953/100‑1 (MQ=255)

                                                                    cagcaAAGGTCACGCGACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                           <  1:3952133/100‑1 (MQ=255)

                                                                     agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa                          >  2:2187201/1‑100 (MQ=255)

                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                         >  2:2021978/1‑100 (MQ=255)

                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                         >  1:1105456/1‑100 (MQ=255)

                                                                       caAAGGTCACGCCACTAGAACGCCAGTTCGGGCTCTTGATAGAACCAGGACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                        <  2:1670473/100‑1 (MQ=255)

                                                                       caAAGGTCACGCCACCGGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                        <  2:2430505/100‑1 (MQ=255)

                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                       <  1:2328457/100‑1 (MQ=255)

                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                       <  1:2446554/100‑1 (MQ=255)

                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                       >  2:3038958/1‑100 (MQ=255)

                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                        >  2:3384142/1‑99 (MQ=255)

                                                                           ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                    >  2:2319216/1‑100 (MQ=255)

                                                                            gTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAGCGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                     <  2:1881028/98‑1 (MQ=255)

                                                                            gTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACtt                   >  2:777428/1‑100 (MQ=255)

                                                                             tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                  <  1:1235795/100‑1 (MQ=255)

                                                                              cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                 >  2:2355379/1‑100 (MQ=255)

                                                                                cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc               <  2:3193672/100‑1 (MQ=255)

                                                                                cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc               >  2:1659888/1‑100 (MQ=255)

                                                                                 gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa              <  2:1970428/100‑1 (MQ=255)

                                                                                 gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa              <  1:1518800/100‑1 (MQ=255)

                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc             <  1:711684/100‑1 (MQ=255)

                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc             >  2:454168/1‑100 (MQ=255)

                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc             <  1:354555/100‑1 (MQ=255)

                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc             >  1:3390369/1‑100 (MQ=255)

                                                                                     ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg          >  1:992533/1‑100 (MQ=255)

                                                                                     ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg          >  1:957696/1‑100 (MQ=255)

                                                                                     ccaGAACGCCAGTGCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg          >  2:3909730/1‑100 (MQ=255)

                                                                                      caGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt         >  2:591930/1‑100 (MQ=255)

                                                                                       aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc        <  1:2172819/100‑1 (MQ=255)

                                                                                       aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc        <  1:3650557/100‑1 (MQ=255)

                                                                                        gAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct       <  2:3531639/100‑1 (MQ=255)

                                                                                          aCGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc     >  1:3610523/1‑100 (MQ=255)

                                                                                            gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg   <  1:477621/100‑1 (MQ=255)

                                                                                            gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg   <  1:663611/100‑1 (MQ=255)

CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGA  >  NC_000913/3451960‑3452152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`