Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 488,815 G→A 77.2% A760A (GCG→GCA mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913488,8150GA77.2% 280.8 / 87.8 157A760A (GCG→GCAmscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base G (14/22);  new base A (57/64);  total (71/86)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 158 total aligned reads displayed.

AACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCG  >  NC_000913/488716‑488910
                                                                                                   |                                                                                               
aaCCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGgcg                                                                                                 <  2:492290/100‑1 (MQ=255)
   cGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGt                                                                                              >  2:954516/1‑100 (MQ=255)
   cGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGt                                                                                              >  1:1713370/1‑100 (MQ=255)
     tttATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCg                                                                                            <  1:343584/100‑1 (MQ=255)
      ttATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCgg                                                                                           <  1:1089590/100‑1 (MQ=255)
      ttATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCgg                                                                                           >  2:775452/1‑100 (MQ=255)
       tctCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGc                                                                                          <  1:3781448/98‑1 (MQ=255)
       tacctgctgATCAGCTGGAACCTGCTGTACCAGACGGTACGGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGc                                                                                          <  2:2250514/97‑1 (MQ=255)
       tATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGc                                                                                          <  2:2392410/100‑1 (MQ=255)
       tATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGc                                                                                          <  2:824687/100‑1 (MQ=255)
        aTCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGc                                                                                          <  2:2462006/99‑1 (MQ=255)
         tCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAg                                                                                        >  1:2496945/1‑100 (MQ=255)
            ggTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAAt                                                                                     >  2:3507349/1‑100 (MQ=255)
            ggTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAAt                                                                                     >  2:3882013/1‑100 (MQ=255)
             gTGATCCTCTGGCACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGCATCGCCTCGCGTCGTGCGCTCGCACGTCGGCAgcccc                                                                                    >  2:2788309/1‑96 (MQ=255)
             gTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATc                                                                                    >  1:602806/1‑100 (MQ=255)
              tGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCt                                                                                   >  1:2285752/1‑100 (MQ=255)
                atcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTgg                                                                                 <  1:737796/100‑1 (MQ=255)
                atcatcGGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGGGGCGTATCTCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTgg                                                                                 <  2:4157792/100‑1 (MQ=255)
                 tcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGt                                                                                <  2:1748113/100‑1 (MQ=255)
                 tcatcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGt                                                                                >  1:2000673/1‑100 (MQ=255)
                  catcTGGAACTTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGTCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTg                                                                               <  2:2743434/100‑1 (MQ=255)
                  catcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTg                                                                               <  2:231925/100‑1 (MQ=255)
                  catcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTg                                                                               <  1:2421206/100‑1 (MQ=255)
                   atcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGa                                                                              >  1:1065664/1‑100 (MQ=255)
                   atcTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGa                                                                              >  1:253124/1‑100 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAaga                                                                          >  1:731946/1‑100 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAaga                                                                          >  1:293372/1‑100 (MQ=255)
                       ggAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCATCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAaga                                                                          >  1:3905208/1‑100 (MQ=255)
                        ggaCCTGCCGGACCAGTCGGTACTGCTTGGCTTTAGCGTAGCTGCGCGGCGTATCGCCTGGCGTCGGGCGCTGGCACGTCGGCAGAATCTGGTGAAagag                                                                         <  2:204702/98‑1 (MQ=255)
                         aaCCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAgg                                                                        >  1:776377/1‑100 (MQ=255)
                          aCCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAggg                                                                       <  2:3214574/100‑1 (MQ=255)
                            ctgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGgcg                                                                     >  2:426612/1‑100 (MQ=255)
                             tgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGgcgc                                                                    <  2:2489371/100‑1 (MQ=255)
                             tgctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGgcgc                                                                    <  1:2087357/100‑1 (MQ=255)
                               ctgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAg                                                                  <  1:2333055/100‑1 (MQ=255)
                               ctgTACCAGACGGTACTGCGTGGATTAAGCGTAGCGGCGAGGCGTATCGCCTGGCGTCTTACGCTGGCACGCAGGAAGAATATGGTGAAAGAAGGCGCat                                                                  >  2:3864260/1‑99 (MQ=255)
                                tgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGa                                                                 >  2:2841007/1‑100 (MQ=255)
                                tgTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGa                                                                 >  2:1131014/1‑100 (MQ=255)
                                 gTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGaa                                                                <  1:1941133/100‑1 (MQ=255)
                                 gTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGaa                                                                >  1:3814615/1‑100 (MQ=255)
                                   aCCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAgg                                                              >  1:280161/1‑100 (MQ=255)
                                   aCCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAgg                                                              >  1:1875829/1‑100 (MQ=255)
                                    ccAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATTGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGt                                                             >  1:3928615/1‑100 (MQ=255)
                                    ccAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGt                                                             >  2:1294829/1‑100 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGc                                                           <  1:425323/100‑1 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGc                                                           >  1:1897118/1‑100 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGc                                                           >  1:1530291/1‑100 (MQ=255)
                                      aGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGc                                                           >  1:1053996/1‑100 (MQ=255)
                                       gACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCt                                                          <  1:4083969/100‑1 (MQ=255)
                                       gACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCt                                                          <  1:2886069/100‑1 (MQ=255)
                                         cGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGa                                                        <  1:3653353/100‑1 (MQ=255)
                                           gTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAc                                                      >  1:3290110/1‑100 (MQ=255)
                                             aCTGCGTGGCTTAAGCGTCGCGGCGCGGCGTATCGCCTGGCGCCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAcc                                                     <  2:1767277/99‑1 (MQ=255)
                                             aCTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccg                                                    <  1:3954800/100‑1 (MQ=255)
                                             aCTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccg                                                    <  2:1389637/100‑1 (MQ=255)
                                               tGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgcc                                                  >  2:1126712/1‑100 (MQ=255)
                                                gCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAccgccg                                                 >  1:3439364/1‑100 (MQ=255)
                                                   tGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaa                                              <  1:2446474/100‑1 (MQ=255)
                                                    ggCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaag                                             <  1:440639/100‑1 (MQ=255)
                                                    ggCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCCTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaa                                              >  2:2219381/1‑99 (MQ=255)
                                                     ggttACGCGTAGCGGCGCGGCGGATCGCCTGGCGTCGTGCGGGGGCAGGTCGGCAGAATCTGGCGAAAGAGGGCGGAGAAGGTGCTGAACAGCCGgaag                                             <  2:3774763/97‑1 (MQ=255)
                                                     gCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaaga                                            >  2:1144770/1‑100 (MQ=255)
                                                      nttAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaagaa                                           >  2:574228/2‑100 (MQ=255)
                                                      cTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGgaagaa                                           >  1:229280/1‑100 (MQ=255)
                                                      cTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCACCGgaagaa                                           >  1:919548/1‑100 (MQ=255)
                                                          aGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACcca                                       >  1:1749703/1‑100 (MQ=255)
                                                           gCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACccac                                      <  1:37440/100‑1 (MQ=255)
                                                             gTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACccacca                                    >  1:2013550/1‑100 (MQ=255)
                                                              tAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCAt                                   >  2:2634991/1‑100 (MQ=255)
                                                               aGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAAGCTGGTGAAAGAGGGCGCAGAAGGTGCGGAACCGCCGGAAGAACCCACCAtt                                  >  2:47122/1‑100 (MQ=255)
                                                                 cggcgcggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGc                                >  1:2141101/1‑100 (MQ=255)
                                                                  ggcgcggcgTATCACCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGc                                <  2:306152/99‑1 (MQ=255)
                                                                   gcgcggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCAc                              <  2:191720/100‑1 (MQ=255)
                                                                      cggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTgg                           <  1:2335927/100‑1 (MQ=255)
                                                                       ggcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGa                          >  1:956076/1‑100 (MQ=255)
                                                                        gcgTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAg                         >  1:1360500/1‑100 (MQ=255)
                                                                        gcgTATCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAg                         <  1:3827865/100‑1 (MQ=255)
                                                                            aTCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAg                     <  1:732842/100‑1 (MQ=255)
                                                                            aTCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAg                     <  1:1182423/100‑1 (MQ=255)
                                                                            aTCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCACAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAg                     <  1:1077669/100‑1 (MQ=255)
                                                                             tCGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGt                    >  2:1577864/1‑100 (MQ=255)
                                                                              cGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGtt                   <  2:3303471/100‑1 (MQ=255)
                                                                              cGCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGtt                   <  2:354870/100‑1 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                  >  2:2150861/1‑100 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                  >  1:1628318/1‑100 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                  >  2:1535170/1‑100 (MQ=255)
                                                                               gCCTGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTa                  <  2:2994213/100‑1 (MQ=255)
                                                                                  tGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc               >  1:2826396/1‑100 (MQ=255)
                                                                                  tGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc               <  2:318375/100‑1 (MQ=255)
                                                                                  tGGCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc               <  1:2899046/100‑1 (MQ=255)
                                                                                    gCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAACGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACca              <  2:567735/99‑1 (MQ=255)
                                                                                    gCGTCGTGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcag             >  2:4062198/1‑100 (MQ=255)
                                                                                      gtcgtGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagca           <  1:2469215/100‑1 (MQ=255)
                                                                                       tcgtGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagcag          <  1:1242222/100‑1 (MQ=255)
                                                                                         gtGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGAc        >  1:548300/1‑100 (MQ=255)
                                                                                         gtGCGCTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGAc        >  1:2205360/1‑100 (MQ=255)
                                                                                           gcgcTGGCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGc      <  1:3297524/100‑1 (MQ=255)
                                                                                             gcTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACTATAGCACTGGAGCAAGTTAACCAGCAGACGCTg    <  2:2737095/100‑1 (MQ=255)
                                                                                                ggCACGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCg  <  2:1268101/99‑1 (MQ=255)
                                                                                                   |                                                                                               
AACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCG  >  NC_000913/488716‑488910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: