Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,678,937 T→A 16.1% Q84L (CAG→CTG)  yhjH ← cyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,678,9370TA16.1% 517.6 / 79.4 204Q84L (CAG→CTG) yhjHcyclic‑di‑GMP phosphodiesterase, FlhDC‑regulated
Reads supporting (aligned to +/- strand):  ref base T (90/81);  new base A (15/18);  total (105/99)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 208 total aligned reads displayed.

TGCTGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGACCACCG  >  NC_000913/3678838‑3679036
                                                                                                   |                                                                                                   
 gCTGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGTGCCAGCAAATCAATCTg                                                                                                    >  1:3872173/1‑100 (MQ=255)
  cTGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGc                                                                                                   <  1:2259859/100‑1 (MQ=255)
  cTGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGc                                                                                                   >  2:2652130/1‑100 (MQ=255)
   tGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGct                                                                                                  <  1:3227840/100‑1 (MQ=255)
    gccGCAGGGCGATGAGCGTAGGGCCATCAATATTGTCCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGct                                                                                                  <  2:4172245/97‑1 (MQ=255)
    gACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctc                                                                                                 >  2:2565237/1‑100 (MQ=255)
    gACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctc                                                                                                 >  1:550853/1‑100 (MQ=255)
    gACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctc                                                                                                 >  1:2964995/1‑100 (MQ=255)
    gACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctc                                                                                                 <  2:1981923/100‑1 (MQ=255)
    gACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctc                                                                                                 >  2:1445086/1‑100 (MQ=255)
     aCGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGctct                                                                                                <  2:1388528/100‑1 (MQ=255)
      cGCAGGGCGATGAGCGTAGGGCCCTCAATATTTACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCtt                                                                                               >  1:1483511/1‑100 (MQ=255)
        cAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTc                                                                                             <  1:2886311/100‑1 (MQ=255)
         aGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTca                                                                                            <  1:3500690/100‑1 (MQ=255)
         aGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTca                                                                                            <  2:1497093/100‑1 (MQ=255)
         aGGACGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTca                                                                                            >  1:3483316/1‑100 (MQ=255)
          gggCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTcac                                                                                           <  1:2123963/100‑1 (MQ=255)
          gggCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTcac                                                                                           >  2:55101/1‑100 (MQ=255)
           ggCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTcaca                                                                                          >  1:2913198/1‑100 (MQ=255)
            gCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACaa                                                                                         <  2:1316086/100‑1 (MQ=255)
              gATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAAcc                                                                                       <  1:3745142/100‑1 (MQ=255)
               aTGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCt                                                                                      >  1:773074/1‑100 (MQ=255)
                 gAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTcc                                                                                    <  1:103909/100‑1 (MQ=255)
                   gCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCAt                                                                                  <  1:3920970/100‑1 (MQ=255)
                     gcgggggCATCAATATCGCCCGATGCCAGCAGGCCGTGCACTATAAAGGAGTCGGCAGTCTTCGCCAGCAAATCACTCTGCTCTTCCACAAcc                                                                                       <  2:1662814/90‑1 (MQ=255)
                     gTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATcc                                                                                <  2:775899/100‑1 (MQ=255)
                     gTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATcc                                                                                >  1:1081676/1‑100 (MQ=255)
                       aGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGa                                                                              <  2:2030976/100‑1 (MQ=255)
                           ccATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGc                                                                          <  2:19240/100‑1 (MQ=255)
                           ccATCAATATTGACCGATGCCAGCAGGCCGTGCTCTAAAAAGAAGTCGGCTTTTCGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATACGATGGc                                                                          >  2:2233340/1‑100 (MQ=255)
                            cATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCt                                                                         >  1:2790827/1‑100 (MQ=255)
                            cATCAATATTGACCGATGCCAGCAGCCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGc                                                                          <  1:2510087/99‑1 (MQ=255)
                             aTCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTg                                                                        >  2:2461005/1‑100 (MQ=255)
                              tCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGa                                                                       >  1:3174802/1‑100 (MQ=255)
                                aaTATTGACCGATGCAAGCACGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACg                                                                     <  1:2296848/100‑1 (MQ=255)
                                 atatTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACgg                                                                    >  1:1498230/1‑100 (MQ=255)
                                   atTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTg                                                                  >  1:2531562/1‑100 (MQ=255)
                                   atTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTg                                                                  <  2:3527216/100‑1 (MQ=255)
                                   atTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTg                                                                  <  2:2606299/100‑1 (MQ=255)
                                    ttGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGa                                                                 <  1:1798912/100‑1 (MQ=255)
                                      naCCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGAtt                                                               >  2:2815686/2‑100 (MQ=255)
                                      gACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGAtt                                                               <  1:1924185/100‑1 (MQ=255)
                                      gACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGAtt                                                               >  1:3262692/1‑100 (MQ=255)
                                        ccGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTc                                                             <  2:3471494/100‑1 (MQ=255)
                                        ccGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTc                                                             <  1:623144/100‑1 (MQ=255)
                                          gATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTTTTTTTTCTTATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAg                                                           >  2:219980/1‑100 (MQ=255)
                                          gATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAg                                                           <  2:1203523/100‑1 (MQ=255)
                                               cagcagGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTaaaa                                                      >  1:114219/1‑100 (MQ=255)
                                                   agGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGc                                                  <  2:1827965/100‑1 (MQ=255)
                                                    ggCCGTGCTCTATAAAGAAGTCGGCTTTTTTTTTTTTTTTTTCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAACTa                                                    >  2:355489/1‑97 (MQ=255)
                                                    ggCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCg                                                 <  1:867210/100‑1 (MQ=255)
                                                      ccGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGAt                                               <  2:2407850/100‑1 (MQ=255)
                                                      ccGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGAt                                               <  1:1567813/100‑1 (MQ=255)
                                                            tctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcg                                         >  1:1345334/1‑100 (MQ=255)
                                                            tctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcg                                         >  1:352014/1‑100 (MQ=255)
                                                             ctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcgg                                        <  1:2566751/100‑1 (MQ=255)
                                                             ctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcgg                                        >  2:3654011/1‑100 (MQ=255)
                                                             ctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcgg                                        <  1:1198883/100‑1 (MQ=255)
                                                             ctATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCAGATCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCcggcg                                         >  2:160003/1‑99 (MQ=255)
                                                              tataAAGAAGTCGGCTTTTTGCGCCATCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATCGCGATCCgacggc                                       >  2:1793025/1‑100 (MQ=255)
                                                                taAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAg                                     <  2:2913182/100‑1 (MQ=255)
                                                                taAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAg                                     >  1:831625/1‑100 (MQ=255)
                                                                 aaaGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAgg                                    <  2:3022959/100‑1 (MQ=255)
                                                                  aagaagTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGc                                   <  1:3529245/100‑1 (MQ=255)
                                                                      agTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTg                               >  1:278026/1‑100 (MQ=255)
                                                                       gTCGGCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGc                              <  2:2340732/100‑1 (MQ=255)
                                                                        tCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCg                             >  1:1009719/1‑100 (MQ=255)
                                                                         cGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGa                            <  2:2132079/100‑1 (MQ=255)
                                                                         cGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGa                            >  2:3991001/1‑100 (MQ=255)
                                                                           gCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAg                          <  2:2528866/100‑1 (MQ=255)
                                                                           gCTTTTTGCGCCAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAg                          <  2:734798/100‑1 (MQ=255)
                                                                             tttttGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAggg                        <  2:3737552/100‑1 (MQ=255)
                                                                             tttttGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAggg                        >  1:903682/1‑100 (MQ=255)
                                                                             tttttGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAggg                        >  1:89035/1‑100 (MQ=255)
                                                                             tttttGCGCCAGCAAATCAATCTGCTCTTTCACAACCGCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAggg                        <  2:3780948/100‑1 (MQ=255)
                                                                              ttttGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGt                       <  1:3665040/100‑1 (MQ=255)
                                                                              ttttGCGCCAGCAAATCAATATGCTCTTTCACAACCTACATCCGAGGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTACGAAGGGt                       >  2:2743277/1‑100 (MQ=255)
                                                                                 tGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCa                    >  1:3905454/1‑100 (MQ=255)
                                                                                   cgcCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAg                  >  2:1140192/1‑100 (MQ=255)
                                                                                    gcCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAgg                 <  2:3877212/100‑1 (MQ=255)
                                                                                    gcCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAgg                 <  1:1440431/100‑1 (MQ=255)
                                                                                     ccAGCAAATCAATCAGCTCTTTCACAACCTGCATCCGATGGCTGACGGTGATTTCAGTGAAATAGCGAGCCGGCGGCAGGCGTTGCGAAGGGTTCAAgg                 <  2:1029737/99‑1 (MQ=255)
                                                                                     ccAGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAggg                >  1:4112147/1‑100 (MQ=255)
                                                                                       aGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGAt              >  2:437193/1‑100 (MQ=255)
                                                                                       aGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGAt              >  1:695651/1‑100 (MQ=255)
                                                                                       aGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGAt              >  1:2036862/1‑100 (MQ=255)
                                                                                       aGCAAATCAATCAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGAt              <  1:160006/100‑1 (MQ=255)
                                                                                        gCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATg             >  2:2687574/1‑100 (MQ=255)
                                                                                          aaaTCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCg           <  1:3355433/100‑1 (MQ=255)
                                                                                           aatcaatcTGCTCTTTCACCACCTCCATCCGATGGCTGACGGTGCTTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGc            >  2:3596762/1‑98 (MQ=255)
                                                                                            atcaatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTg         <  2:848122/100‑1 (MQ=255)
                                                                                            atcaatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTg         <  2:3542308/100‑1 (MQ=255)
                                                                                            atcaatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGCTCAAGGGATGCGTg         >  2:1103713/1‑100 (MQ=255)
                                                                                             tcaatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGCAGGGTTCAAGGGATGCGTGa        <  2:402292/100‑1 (MQ=255)
                                                                                             tcaatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGa        <  1:437196/100‑1 (MQ=255)
                                                                                             tcaatcAGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGa        <  2:3195895/100‑1 (MQ=255)
                                                                                              caatcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGac       <  2:2515928/100‑1 (MQ=255)
                                                                                                atcTGCTCTTTCACAGCCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGac       <  1:3995768/98‑1 (MQ=255)
                                                                                                 tcTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGGTTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAGGGGTTCAAGGGATGCGTGaccac    >  2:3126430/1‑100 (MQ=255)
                                                                                                   tGCTCTTTTACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGACCACCg  >  2:2307930/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGCTGACGCAGGGCGATGAGCGTAGGGCCATCAATATTGACCGATGCCAGCAGGCCGTGCTCTATAAAGAAGTCGGCTTTTTGCGCCAGCAAATCAATCTGCTCTTTCACAACCTCCATCCGATGGCTGACGGTGATTTCAGTAAAATAGCGATCCGGCGGCAGGCGTTGCGAAGGGTTCAAGGGATGCGTGACCACCG  >  NC_000913/3678838‑3679036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: