Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,701,868 C→A 77.3% R334L (CGT→CTT)  dppF ← dipeptide/heme ABC transporter ATPas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,701,8680CA77.3% 349.5 / 115.4 181R334L (CGT→CTT) dppFdipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base C (23/18);  new base A (71/67);  total (95/86)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 182 total aligned reads displayed.

GCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCG  >  NC_000913/3701769‑3701965
                                                                                                    |                                                                                                 
 ccTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACg                                                                                                  <  1:359329/100‑1 (MQ=255)
    ggCTGCTTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTg                                                                                               >  1:656376/1‑100 (MQ=255)
    ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTg                                                                                               >  2:2460110/1‑100 (MQ=255)
    ggCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTg                                                                                               >  1:2880521/1‑100 (MQ=255)
     gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGc                                                                                              >  1:3649590/1‑100 (MQ=255)
     gCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGc                                                                                              <  1:1663071/100‑1 (MQ=255)
      cTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCg                                                                                             <  1:425094/100‑1 (MQ=255)
      cTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCg                                                                                             <  1:3397939/100‑1 (MQ=255)
       tGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCgg                                                                                            >  1:2994285/1‑100 (MQ=255)
         aTTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGAt                                                                                          >  2:2247603/1‑100 (MQ=255)
          ttCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGAtt                                                                                         >  2:1257468/1‑100 (MQ=255)
            ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGAttt                                                                                        >  2:1988897/1‑99 (MQ=255)
            ctgctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGAtttt                                                                                       >  1:1859579/1‑100 (MQ=255)
              gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCa                                                                                     >  2:3062016/1‑100 (MQ=255)
              gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCa                                                                                     <  2:754530/100‑1 (MQ=255)
              gctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCa                                                                                     >  1:2757302/1‑100 (MQ=255)
              gctgTACGGAATTTTGCAATTCTTGTCGCCAGTGTTCAAGCTTACTGAGTAAACCGGAGCGCATGTCCCCGGTTTTGTGAGTTAAAGTTGCg                                                                                             <  2:122615/92‑1 (MQ=255)
               ctgTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCAt                                                                                    <  1:4108188/100‑1 (MQ=255)
                tgTAAGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATc                                                                                   <  2:1940991/100‑1 (MQ=255)
                 gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATcc                                                                                  <  1:1578087/100‑1 (MQ=255)
                 gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATcc                                                                                  <  1:1067842/100‑1 (MQ=255)
                 gTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGAGTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATcc                                                                                  <  2:805917/100‑1 (MQ=255)
                  tACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCt                                                                                 >  2:876555/1‑100 (MQ=255)
                   aCGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTg                                                                                >  1:700731/1‑100 (MQ=255)
                    cGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGa                                                                               <  2:3365816/100‑1 (MQ=255)
                      gCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATc                                                                             <  2:3976841/100‑1 (MQ=255)
                       cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATc                                                                             <  2:808303/99‑1 (MQ=255)
                       cATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCa                                                                            <  1:11253/100‑1 (MQ=255)
                            tGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGc                                                                       <  1:1614726/100‑1 (MQ=255)
                             gCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCa                                                                      >  1:618989/1‑100 (MQ=255)
                             gCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCa                                                                      >  2:2729986/1‑100 (MQ=255)
                               tATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCaaa                                                                    >  2:213311/1‑100 (MQ=255)
                                aTGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGTCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCaaa                                                                    <  2:4120428/99‑1 (MQ=255)
                                 tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                  >  1:473176/1‑100 (MQ=255)
                                 tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                  >  2:3658865/1‑100 (MQ=255)
                                 tGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                  >  1:3777848/1‑100 (MQ=255)
                                   cTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCAGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAAc                                                                  >  1:3337024/1‑98 (MQ=255)
                                    ttGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAg                                                               <  2:1310773/100‑1 (MQ=255)
                                     tGTCTCCACTGTTGAAGCTGACTGAGTAACCCGGAGCTCATGGCCCCGTTTTTGTAAGTTAAAGCTGCGGATTTTCAGCCTGATCAACAGCAAAACAAGc                                                              <  2:4123333/100‑1 (MQ=255)
                                     tGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                              >  1:1925180/1‑100 (MQ=255)
                                     tGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                              >  2:1737082/1‑100 (MQ=255)
                                     tGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                              <  1:2475937/100‑1 (MQ=255)
                                      gTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                             >  1:1527810/1‑100 (MQ=255)
                                      gTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCt                                                             >  2:2096034/1‑100 (MQ=255)
                                       tCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGc                                                              <  2:395164/98‑1 (MQ=255)
                                        cgacACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                           <  1:2684619/97‑1 (MQ=255)
                                        cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                           >  1:3929028/1‑100 (MQ=255)
                                        cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                           <  2:4008360/100‑1 (MQ=255)
                                        cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                           >  1:748115/1‑100 (MQ=255)
                                        cGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAc                                                           >  2:1841518/1‑100 (MQ=255)
                                         gcgaCTGCTAAAGCTGATTGAGTAAACTGGAGCGCATGGCCCCGGTATTGTGAGTCAACGCTGCGGATTTTCATCCTGATCAACAGTAAACCAAGCTAcc                                                          <  2:1329316/97‑1 (MQ=255)
                                         gCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAcc                                                          >  2:3029436/1‑100 (MQ=255)
                                         gCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTAcc                                                          <  1:4055937/100‑1 (MQ=255)
                                          ccACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCa                                                         <  1:3718860/100‑1 (MQ=255)
                                           cACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAg                                                        >  2:900708/1‑100 (MQ=255)
                                               gTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGa                                                    <  1:1719652/100‑1 (MQ=255)
                                                ttGAAGCTGATTGAGTAAAGCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAc                                                   <  2:1618027/100‑1 (MQ=255)
                                                ttGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAc                                                   <  2:2557848/100‑1 (MQ=255)
                                                 tGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAcc                                                  >  2:1900924/1‑100 (MQ=255)
                                                 tGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCAGCTTCGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACACGCTACCAGTTGAcc                                                  >  2:3597629/1‑100 (MQ=255)
                                                     gCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGCccgcc                                               >  2:4090019/1‑99 (MQ=255)
                                                       tGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTa                                            <  1:3086434/100‑1 (MQ=255)
                                                        gtttGAGTAAACCGGAGCGCATGGTCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAg                                           <  2:1021623/98‑1 (MQ=255)
                                                        ggttGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAg                                           >  2:3206981/3‑100 (MQ=255)
                                                        gATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAg                                           <  2:1527801/100‑1 (MQ=255)
                                                            gAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtt                                       <  1:2646749/100‑1 (MQ=255)
                                                            gAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtt                                       <  1:801838/100‑1 (MQ=255)
                                                             aGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCttt                                      <  1:712494/100‑1 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                     >  1:2129952/1‑100 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                     <  1:3740659/100‑1 (MQ=255)
                                                              gTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCtttt                                     >  1:269907/1‑100 (MQ=255)
                                                               tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                    <  1:3310106/100‑1 (MQ=255)
                                                               tAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTa                                    <  2:4043341/100‑1 (MQ=255)
                                                                aaaCCGGGGCCATGCGCTCCGGTCTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCAGTAGTCGTTTAg                                   >  2:690890/1‑100 (MQ=255)
                                                                aaaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                   >  2:2467116/1‑100 (MQ=255)
                                                                aaaCCGGAGCGCATGGCCCCCGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAg                                   >  2:2549260/1‑100 (MQ=255)
                                                                 aaCCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGc                                  >  2:3551395/1‑100 (MQ=255)
                                                                  aCCGAAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCACCGTAGTCTTTTAGCt                                 >  1:1944881/1‑100 (MQ=255)
                                                                   ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                                >  1:3231991/1‑100 (MQ=255)
                                                                   ccGGAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTg                                >  2:1397885/1‑100 (MQ=255)
                                                                      gAGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCg                              <  2:991426/99‑1 (MQ=255)
                                                                       aGCGCATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGc                            <  2:1169388/100‑1 (MQ=255)
                                                                         cgcATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTg                          <  2:3769937/100‑1 (MQ=255)
                                                                          gcATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGc                         >  2:118373/1‑100 (MQ=255)
                                                                           cATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCa                        <  1:237593/100‑1 (MQ=255)
                                                                           cATGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCa                        >  1:2878404/1‑100 (MQ=255)
                                                                             tGGCCCCGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAAc                      <  2:586520/100‑1 (MQ=255)
                                                                              ggCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACt                     <  1:2617820/100‑1 (MQ=255)
                                                                                gCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTg                    >  2:1242527/1‑100 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                  <  2:2158978/100‑1 (MQ=255)
                                                                                  cccGGTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTggg                  >  1:155342/1‑100 (MQ=255)
                                                                                     ggTTTTGTGAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGc               >  1:1232363/1‑100 (MQ=255)
                                                                                      gTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCa              <  2:463652/100‑1 (MQ=255)
                                                                                        tttGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAgg            <  1:1563259/100‑1 (MQ=255)
                                                                                          tgtgAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAggg           >  1:4133612/1‑99 (MQ=255)
                                                                                           gtgAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGAACGCCGTAGTCTTTTAGCTGCGTCTGCAACTGGGTGCAggg           >  1:908073/1‑98 (MQ=255)
                                                                                            tgAGTTAAAGCTGCGGATTTTCATCCTGATCAACATCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGGGGCTGCAACTGGGTGCAGGGGcc        <  2:1827674/100‑1 (MQ=255)
                                                                                            tgAGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAGCAAGCTACCAGTTGGCCGCCGTGGGCTTTTACCTGCGGCTGCAACTGGGTGCAGGGGcc        <  2:982229/100‑1 (MQ=255)
                                                                                             naGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCg       >  1:229489/2‑100 (MQ=255)
                                                                                              aGTTAAAGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGa      >  1:3875882/1‑100 (MQ=255)
                                                                                                    |                                                                                                 
GCCTGGCTGATTCTGCTGTACGGCATTTTGCTATGCTTGTCGCCACTGTTGAAGCTGATTGAGTAAACCGGAGCGCATGGCCCCGGTTTTGTGAGTTAACGCTGCGGATTTTCATCCTGATCAACAGCAAAACAAGCTACCAGTTGACCGCCGTAGTCTTTTAGCTGCGGCTGCAACTGGGTGCAGGGGCCGAAGCG  >  NC_000913/3701769‑3701965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: