Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,984 T→G 24.0% intergenic (‑194/+114) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG24.0% 587.5 / 198.8 259intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (89/108);  new base G (59/3);  total (148/111)
Fisher's exact test for biased strand distribution p-value = 9.24e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-02

Only 100 of 263 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083
                                                                                                   |                                                                                                        
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                          <  1:750074/100‑1 (MQ=255)
   ctgagggATGGCGCTGCACGCAGAGGCAGCGCGTAAACGGGTGTACAGATTGTTGTCAAAGTGCGATTTGTGTATGGCGTAGCCCGCGTTAACACCttc                                                                                                        <  2:362567/94‑2 (MQ=255)
    tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                      >  1:1776977/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                                  >  1:921884/1‑100 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                 <  2:3184218/100‑1 (MQ=255)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCGCATCCGGc                                                                                                  <  2:1624355/99‑1 (MQ=255)
           tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                               <  2:3786337/100‑1 (MQ=255)
           tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                               >  1:1736440/1‑100 (MQ=255)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                              >  2:348834/1‑100 (MQ=255)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                            >  1:1709447/1‑100 (MQ=255)
              cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGGTAACGCCTTATCCGGCCTACaa                                                                                           >  1:105899/1‑100 (MQ=255)
               gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                          >  1:3664594/1‑100 (MQ=255)
                 tGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                        <  1:3178730/100‑1 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                       <  1:3646792/100‑1 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                      <  1:3253302/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                    <  1:2944035/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGACGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                    >  1:2927163/1‑100 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                   <  2:3907159/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                  <  1:3704685/100‑1 (MQ=255)
                           ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                              >  1:3424797/1‑100 (MQ=255)
                           agCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                              >  2:1754591/2‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                          >  1:4151751/1‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                          <  1:144595/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                          <  2:3087705/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                         <  1:3314644/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCAGCCTACAAAATCGTGCAAATTCAATa                                                                         <  1:2713647/100‑1 (MQ=255)
                                 gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                        >  1:2310467/1‑100 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                    <  2:2903065/97‑1 (MQ=255)
                                          ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTCCAAAATCGTGCAAATTCAATACATTGc                                                                   >  2:2491604/1‑96 (MQ=255)
                                           gTATACAGATTGTTGATAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                            <  2:2437113/100‑1 (MQ=255)
                                             ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑tt                                                        <  1:2488960/100‑1 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGTATTCGGCGTTAACTCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt‑‑ttt                                                       >  1:2268689/1‑100 (MQ=255)
                                               aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTc                                                      <  1:2098404/100‑1 (MQ=255)
                                                cgatctgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCg                                                     <  2:2288436/95‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCg                                                     <  2:2927146/100‑1 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCg                                                     <  2:2268675/100‑1 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTa                                                   >  1:2568812/1‑100 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTa                                                   <  2:4151717/100‑1 (MQ=255)
                                                     tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGc                                                >  2:2981095/1‑100 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             <  1:485548/100‑1 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             >  2:857927/1‑100 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             >  2:110380/1‑100 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             >  2:1890898/1‑100 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             >  2:2378451/1‑100 (MQ=255)
                                                        cgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTg                                             >  2:281776/2‑100 (MQ=255)
                                                         gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGa                                            >  1:2729033/1‑100 (MQ=255)
                                                          aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGAt                                           >  1:3552480/1‑100 (MQ=255)
                                                           cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATa                                          <  2:2646751/100‑1 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCg                                      >  1:3743788/1‑100 (MQ=255)
                                                                tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCgg                                     >  2:75980/1‑100 (MQ=255)
                                                                 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGa                                    >  1:871732/1‑100 (MQ=255)
                                                                  cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGa                                    >  1:233793/1‑99 (MQ=255)
                                                                  cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAg                                   <  2:3729640/100‑1 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAgcg                                 <  1:281779/100‑1 (MQ=255)
                                                                     attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑CCTTGTAGGCCTGATAAGCGTAgcgc                                >  1:1406379/10‑100 (MQ=17)
                                                                      ttGTTTATGCCGGATGCGCCGTTAACGCCTTATCCGGCCTACAAACTCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCa                               <  1:1157764/100‑1 (MQ=255)
                                                                      atttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAGGCCTGATAAGCGTAGCGCAt                              >  1:2671933/9‑100 (MQ=18)
                                                                       tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCAt                              <  2:1924994/100‑1 (MQ=255)
                                                                        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAG‑‑CCACGTAGGCCTGATAAGCGTAGCGCa                               >  1:1294540/7‑98 (MQ=17)
                                                                         tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCa                            <  1:3819240/100‑1 (MQ=255)
                                                                         tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCa                            <  1:735648/100‑1 (MQ=255)
                                                                         tgtaggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGA‑‑TTGCGTAGGCCTGATAAGCGTAGCGCATc                             >  1:3992390/6‑99 (MQ=11)
                                                                          gtaggCCGGATAAGGCGCTTACGCCGCATCCGGCCTACAAGAACGTGCAAATTCAACAAATTGTGATGCAT‑‑CCTGTAGGCCTGATAAGCGTAGCGCATCa                            >  1:1023285/5‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CATGTAGGCCTGATAAGCGTAGCGCATCAgg                          >  1:3892185/4‑100 (MQ=17)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATA‑‑TGACGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:3794943/4‑100 (MQ=18)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTCCAGAAT‑‑TTTCGTAGACCTGATAAGAGGAGCGCATCAgg                          <  2:2567147/100‑1 (MQ=255)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CATGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:3332077/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑CCACGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:1516650/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAG‑‑CTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  2:2057983/2‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc                         >  1:1366761/2‑99 (MQ=18)
                                                                              gtcggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAA‑‑CGATGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                       >  2:1482452/10‑99 (MQ=21)
                                                                              gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAt                       >  2:933234/1‑100 (MQ=255)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCTGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAA‑‑TCATGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                       >  1:1641078/1‑99 (MQ=18)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑ATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCaa                       >  1:3919453/1‑99 (MQ=21)
                                                                               ccGGATGCGGAGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGACCTGATAAGCTGAGCGCATAACGCAtt                      >  2:2547629/1‑100 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                     >  1:2392307/7‑97 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑TTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCacgct                    >  1:2524187/7‑96 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAC‑‑TATGTAGGCCTGATAAGCATAGCGCATCAGGCagctt                    >  1:4016767/7‑96 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGA‑‑TTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAattt                    >  1:3613338/7‑100 (MQ=17)
                                                                                 ggacaaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGAT‑‑TTTCGTAGGCCTGATAAGCGTAGCGCATCAGGCacgct                    >  2:1197906/7‑96 (MQ=17)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGAC‑‑CATGTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt                   >  2:2828621/6‑100 (MQ=18)
                                                                                   aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                  <  2:1699575/100‑1 (MQ=255)
                                                                                    tGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                 >  1:4047714/1‑100 (MQ=255)
                                                                                      aggcgTTAACGCCTTTTTTGGCCTACTAAATCATGCAAATTCAATTCATTGCAGAAT‑‑TTTCGTAGGCCTAATAAGCGGAGCGCATCAGGCATTtttgc                 >  2:3554594/2‑98 (MQ=18)
                                                                                       ngcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATCtttgcctt              >  2:1041620/2‑100 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt              <  2:11452/100‑1 (MQ=255)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg            <  2:320447/100‑1 (MQ=255)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg            <  2:481015/100‑1 (MQ=255)
                                                                                         cgTTAACGCCTTATCCGACCAACAAATTATGCAACTCCACAATATTGCAGAAA‑‑TTAAGTAGGACTTATAAGCATAACGCATAAAACAtttattcgttt             >  2:329578/1‑90 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTTGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           <  2:883385/100‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc           <  1:3215199/100‑1 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa         >  1:4091024/1‑100 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt        >  1:3365096/1‑100 (MQ=255)
                                                                                              aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc       >  1:3396360/1‑100 (MQ=255)
                                                                                               cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAACGCATCAGGCATTTTTGCCTTTGCCATCa      >  2:3967615/1‑100 (MQ=255)
                                                                                                gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCan     <  2:535133/100‑2 (MQ=255)
                                                                                                gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg     >  1:259524/1‑100 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    >  1:3514199/1‑100 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    >  1:3105986/1‑100 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    >  1:3015510/1‑100 (MQ=255)
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TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: