Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,802,190 T→C 78.9% K290R (AAA→AGA)  waaO ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,802,1900TC78.9% 354.1 / 84.8 170K290R (AAA→AGA) waaOUDP‑D‑galactose:(glucosyl)lipopolysaccharide‑ alpha‑1,3‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (12/24);  new base C (51/83);  total (63/107)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

Only 100 of 172 total aligned reads displayed.

ATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTG  >  NC_000913/3802093‑3802285
                                                                                                 |                                                                                               
atatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCAtttttt                                                                                               <  1:1926944/100‑1 (MQ=255)
 tatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTg                                                                                              <  2:3999867/100‑1 (MQ=255)
 tatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTg                                                                                              >  1:3680749/1‑100 (MQ=255)
 tatCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTg                                                                                              <  1:2772139/100‑1 (MQ=255)
   tCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGcc                                                                                            <  1:2533977/100‑1 (MQ=255)
   tCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGcc                                                                                            <  2:595774/100‑1 (MQ=255)
   tCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGcc                                                                                            >  1:409253/1‑100 (MQ=255)
    cTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCt                                                                                           <  1:2116491/100‑1 (MQ=255)
     tATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTc                                                                                          <  1:2146039/100‑1 (MQ=255)
     tATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATCTCTTGCCTc                                                                                          >  1:1340268/1‑100 (MQ=255)
     tATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCAGTATTCTTCCATGGCGAAGCATTTCTTGCCTc                                                                                          >  2:4109331/1‑100 (MQ=255)
      aTGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGcc                                                                                            <  1:1082475/97‑1 (MQ=255)
      aTGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTcc                                                                                         >  1:1012652/1‑100 (MQ=255)
       tactctttAAGCATATGTTTTGCGCTAAATTTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTc                                                                                          <  2:3690151/93‑1 (MQ=255)
       tGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCa                                                                                        <  1:1876509/100‑1 (MQ=255)
       tGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCa                                                                                        >  1:3845453/1‑100 (MQ=255)
        gCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCAtttttttttttttt                                                                                       >  2:1747872/1‑92 (MQ=255)
          tttttAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATaa                                                                                     >  2:2415645/1‑100 (MQ=255)
            tttAAGCATATTTTTTGCTCTATAGCTTAATTGATTGCTATTGTTCGGTTTCATCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAg                                                                                   <  2:1912526/100‑1 (MQ=255)
                aGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCtt                                                                               >  1:844226/1‑100 (MQ=255)
                  cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTtg                                                                              <  1:2259950/99‑1 (MQ=255)
                  cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgt                                                                             >  1:3118640/1‑100 (MQ=255)
                  cATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgt                                                                             >  2:1403875/1‑100 (MQ=255)
                   atatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTtgtg                                                                            <  1:1594377/100‑1 (MQ=255)
                    tatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGa                                                                           <  2:37177/100‑1 (MQ=255)
                    tatGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGa                                                                           <  1:1719895/100‑1 (MQ=255)
                        ttttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACa                                                                       <  2:1927117/100‑1 (MQ=255)
                         tttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAg                                                                      <  1:2416836/100‑1 (MQ=255)
                          ttGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAgg                                                                     <  1:3651286/100‑1 (MQ=255)
                           tGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCGTTTTTTGCCTCCATAAAGGCTTGTGATACAGGa                                                                    <  2:2495470/100‑1 (MQ=255)
                            gcgcTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGAt                                                                   <  1:3395697/100‑1 (MQ=255)
                             cgcTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATa                                                                  <  1:3361646/100‑1 (MQ=255)
                              gcTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATaa                                                                 >  1:1788702/1‑100 (MQ=255)
                               cTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAAt                                                                >  2:1611694/1‑100 (MQ=255)
                                tataTCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATc                                                               <  2:1721253/100‑1 (MQ=255)
                                   atCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCa                                                            <  1:3227203/100‑1 (MQ=255)
                                   atCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCa                                                            >  1:1493320/1‑100 (MQ=255)
                                    tCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAg                                                           >  1:1993449/1‑100 (MQ=255)
                                      ttAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGc                                                         <  1:2626149/100‑1 (MQ=255)
                                       tAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGcc                                                        >  1:3824915/1‑100 (MQ=255)
                                        aaTTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGccc                                                       <  2:282844/100‑1 (MQ=255)
                                        aaTTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGccc                                                       <  2:282780/100‑1 (MQ=255)
                                           tgattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAAt                                                    <  1:3617968/100‑1 (MQ=255)
                                           tgattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAAt                                                    <  1:2292337/100‑1 (MQ=255)
                                            gattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATc                                                   <  1:2067939/100‑1 (MQ=255)
                                            gattgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATc                                                   >  1:772396/1‑100 (MQ=255)
                                               tgCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATg                                                <  2:3076997/100‑1 (MQ=255)
                                                gCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGc                                               >  2:2546602/1‑100 (MQ=255)
                                                 cTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGcc                                              >  1:735137/1‑100 (MQ=255)
                                                  tATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCa                                             <  2:1525931/100‑1 (MQ=255)
                                                   aTTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAg                                            >  2:753475/1‑100 (MQ=255)
                                                   aTTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAg                                            <  1:2766478/100‑1 (MQ=255)
                                                     tGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAggg                                          >  2:104121/1‑100 (MQ=255)
                                                     tGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAggg                                          >  2:105616/1‑100 (MQ=255)
                                                      gTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGc                                         >  1:2559136/1‑100 (MQ=255)
                                                      gTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGc                                         <  2:4053318/100‑1 (MQ=255)
                                                      gTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGc                                         <  2:1840578/100‑1 (MQ=255)
                                                       ttCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCt                                        >  1:1099539/1‑100 (MQ=255)
                                                        tCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCtt                                       <  2:3745294/100‑1 (MQ=255)
                                                          ggTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttgg                                     <  1:3116034/100‑1 (MQ=255)
                                                           gTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggt                                    <  2:1387203/100‑1 (MQ=255)
                                                            tttCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggtt                                   <  1:2420070/100‑1 (MQ=255)
                                                              tCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAATGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCttggttgg                                 <  1:2346302/100‑1 (MQ=255)
                                                               cAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGc                                >  2:415492/1‑100 (MQ=255)
                                                               cAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATACCAGGCCCAATCATGCCAGGGCTTGGTTGGc                                >  2:3877480/1‑100 (MQ=255)
                                                               cAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGc                                >  2:3781842/1‑100 (MQ=255)
                                                               cAGCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCACAGGCCCAATCATGCCAGGGCTTGGTTGGc                                >  1:3994298/1‑100 (MQ=255)
                                                                aGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGcc                               <  1:4088493/100‑1 (MQ=255)
                                                                 gCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                              <  1:3240240/100‑1 (MQ=255)
                                                                 gCAACGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGccc                              <  1:196871/100‑1 (MQ=255)
                                                                   aaCGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGa                            <  1:1561115/100‑1 (MQ=255)
                                                                    aCGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGat                           >  2:3392004/1‑100 (MQ=255)
                                                                    aCGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGat                           <  1:1296996/100‑1 (MQ=255)
                                                                     cGCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGata                          <  1:2911858/100‑1 (MQ=255)
                                                                      gCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGatat                         >  2:2063178/1‑100 (MQ=255)
                                                                      gCCGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGatat                         >  2:3011576/1‑100 (MQ=255)
                                                                       ccGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGAtata                        >  1:815688/1‑100 (MQ=255)
                                                                        cGTATTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATaa                       >  1:3413932/1‑100 (MQ=255)
                                                                          tATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATg                     <  1:1016945/100‑1 (MQ=255)
                                                                           aTTCTTCCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATgg                    <  1:3462016/100‑1 (MQ=255)
                                                                            ttcttcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGa                   >  2:1162979/1‑100 (MQ=255)
                                                                             tcttcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGAt                  <  1:365638/100‑1 (MQ=255)
                                                                              cttcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCCGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATa                 >  2:2672389/1‑100 (MQ=255)
                                                                              cttcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATa                 <  1:3543420/100‑1 (MQ=255)
                                                                              cttcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATa                 >  1:3332834/1‑100 (MQ=255)
                                                                                tcCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATaaa               >  1:2018065/1‑100 (MQ=255)
                                                                                tcCATGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATaaa               >  2:330685/1‑100 (MQ=255)
                                                                                   aTGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAAt            >  1:2392888/1‑100 (MQ=255)
                                                                                   aTGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAAt            <  1:1709586/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa           <  2:1249697/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa           <  1:3670242/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa           <  1:619635/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa           <  2:3182637/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATa           <  1:233393/100‑1 (MQ=255)
                                                                                    tGGCGAAGCATTTCTTCCCTCCATAAAGGCTTGTGATACAGAATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGTATAAAAATa           <  2:512478/100‑1 (MQ=255)
                                                                                     ggCGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAg          >  2:3497219/1‑100 (MQ=255)
                                                                                       cGAAGCATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTa        <  1:2641003/100‑1 (MQ=255)
                                                                                            cATTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGtt   >  2:3183058/1‑100 (MQ=255)
                                                                                             aTTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTg  <  2:409250/100‑1 (MQ=255)
                                                                                             aTTTCTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTg  <  1:1075290/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATATCTATGCTTTTTAAGCATATGTTTTGCGCTATATCTTAATTGATTGCTATTGTTCGGTTTCAGCAACGCCGTATTCTTCCATGGCGAAGCATTTTTTGCCTCCATAAAGGCTTGTGATACAGGATAATCCCAGGCCCAATCATGCCAGGGCTTGGTTGGCCCGATATAATGGATAAAAATAGTATCGTTG  >  NC_000913/3802093‑3802285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: