BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,910,662	A→T	100%	D288E (GAT→GAA)	pstS ←	phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,662	0	A	T	100.0%	669.1 / NA	195	D288E (GAT→GAA)	pstS	phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (96/99); total (96/99)

Only 100 of 195 total aligned reads displayed.

GGCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910565‑3910758

ggCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTc                                                                                                >  1:2430163/1‑100 (MQ=255)

ggCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTc                                                                                                <  2:2511693/100‑1 (MQ=255)

  cTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCtt                                                                                              >  1:477011/1‑100 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:1202203/100‑1 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          >  1:1628769/1‑100 (MQ=255)

      cGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTtgtg                                                                                          <  2:1662312/100‑1 (MQ=255)

        tAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGGCCGGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGa                                                                                        <  2:3735779/100‑1 (MQ=255)

          aTCCAGGTCGTTCGTCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATc                                                                                      <  1:3904895/100‑1 (MQ=255)

          aTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATc                                                                                      <  1:742819/100‑1 (MQ=255)

          aTCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATc                                                                                      <  2:4086670/100‑1 (MQ=255)

           tCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCa                                                                                     <  1:2952568/100‑1 (MQ=255)

           tCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCa                                                                                     <  2:2799357/100‑1 (MQ=255)

            ccAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAg                                                                                    <  1:757519/100‑1 (MQ=255)

             cAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTTCGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTTGATCAg                                                                                    >  2:2973355/1‑99 (MQ=255)

              aGGTCGTTCGCCTGTTTAGCCACGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGaa                                                                                  >  2:1655750/1‑100 (MQ=255)

               ggTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAAt                                                                                 >  1:3066567/1‑100 (MQ=255)

                gTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATg                                                                                <  1:3379059/100‑1 (MQ=255)

                 tCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGa                                                                               >  1:3345047/1‑100 (MQ=255)

                  cGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGaa                                                                              >  1:4083449/1‑100 (MQ=255)

                    ttCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACg                                                                            <  1:2449181/100‑1 (MQ=255)

                    ttCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACg                                                                            <  2:3725509/100‑1 (MQ=255)

                      cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          >  2:1604154/1‑100 (MQ=255)

                      cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTg                                                                          <  2:684648/100‑1 (MQ=255)

                      cGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAAAGTg                                                                          >  1:2800786/1‑100 (MQ=255)

                        ccTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGt                                                                        >  2:3418419/1‑100 (MQ=255)

                         cTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTa                                                                       >  1:1034277/1‑100 (MQ=255)

                          tGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTag                                                                      <  2:3066549/100‑1 (MQ=255)

                           gTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTaga                                                                     >  1:791207/1‑100 (MQ=255)

                           gTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTaga                                                                     <  1:3134264/100‑1 (MQ=255)

                            tttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAGTGAACGTGGTagag                                                                    <  1:3215173/100‑1 (MQ=255)

                            tttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTagag                                                                    >  1:857794/1‑100 (MQ=255)

                            tttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTagag                                                                    >  1:3086904/1‑100 (MQ=255)

                             ttAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAgg                                                                   <  1:582109/100‑1 (MQ=255)

                              tAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGt                                                                  <  1:1278745/100‑1 (MQ=255)

                                 ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  2:1137178/1‑100 (MQ=255)

                                 ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  2:3663004/1‑100 (MQ=255)

                                 ccccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAAt                                                               >  2:2989892/1‑100 (MQ=255)

                                   ccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATa                                                              >  1:1282997/1‑99 (MQ=255)

                                   ccGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAg                                                             >  2:1017340/1‑100 (MQ=255)

                                    cGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAgg                                                            <  1:4083437/100‑1 (MQ=255)

                                    cGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAgg                                                            <  1:1996555/100‑1 (MQ=255)

                                     ggTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGc                                                           <  2:2387874/100‑1 (MQ=255)

                                       ttttGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCa                                                         <  2:816519/100‑1 (MQ=255)

                                           gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     >  2:3844220/1‑100 (MQ=255)

                                           gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     >  2:889692/1‑100 (MQ=255)

                                           gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     >  1:3599631/1‑100 (MQ=255)

                                           gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     <  1:1017344/100‑1 (MQ=255)

                                           gTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCa                                                     <  1:2176260/100‑1 (MQ=255)

                                            tACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCTATGc                                                      >  2:2030548/1‑98 (MQ=255)

                                               gCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCtt                                                 >  1:394113/1‑100 (MQ=255)

                                                   aGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGcc                                             <  2:1235744/100‑1 (MQ=255)

                                                    gTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCt                                            >  2:3288123/1‑100 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  1:1086366/1‑100 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  2:3104567/1‑100 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTc                                         >  2:1705722/1‑100 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATATTCGCCttt                                          >  2:902140/1‑99 (MQ=255)

                                                       gaagaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTCCTGTTCTTTGTGGTTCATAATGAACATAGTATAGGTAATATGCCATGCATCTTCGGCTTTc                                         >  2:2570411/1‑100 (MQ=255)

                                                         agaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtg                                       >  1:490525/1‑100 (MQ=255)

                                                         agaaTTTCAGCACTTCTGTGCCTGGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtg                                       <  1:1803621/100‑1 (MQ=255)

                                                          gaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtgg                                      <  2:595713/100‑1 (MQ=255)

                                                          gaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtgg                                      >  1:402668/1‑100 (MQ=255)

                                                          gaaTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtgg                                      <  2:2145670/100‑1 (MQ=255)

                                                            aTTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggtt                                    >  2:3591205/1‑100 (MQ=255)

                                                             tttCAGCACTTCTGTTCCTTTTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGATCGTGGTAGAGGTAATAGGCCATGCATCTTCTCCTTTCtggttg                                   <  2:3261385/100‑1 (MQ=255)

                                                             tttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttg                                   <  2:2067143/100‑1 (MQ=255)

                                                              ttCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttgg                                  <  1:1356671/100‑1 (MQ=255)

                                                               tCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCtggttggt                                 >  1:2443376/1‑100 (MQ=255)

                                                                cAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTc                                >  1:1195135/1‑100 (MQ=255)

                                                                cAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTc                                <  1:2615844/100‑1 (MQ=255)

                                                                 aGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCa                               >  2:4153991/1‑100 (MQ=255)

                                                                  gCACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAg                              <  2:1308884/100‑1 (MQ=255)

                                                                   cACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCCTCTTCGCCTTTCTGGTTGGTCAGa                             >  2:3917888/1‑100 (MQ=255)

                                                                   cACTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGa                             >  1:2295327/1‑100 (MQ=255)

                                                                    aCTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGAt                            >  1:1978084/1‑100 (MQ=255)

                                                                     cTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGAt                            <  1:3283913/99‑1 (MQ=255)

                                                                     cTTCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATc                           <  2:760481/100‑1 (MQ=255)

                                                                      ttCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATcc                          <  1:971690/100‑1 (MQ=255)

                                                                       tCTGTGCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCt                         >  2:2419639/1‑100 (MQ=255)

                                                                         tgtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGa                       >  1:144464/1‑100 (MQ=255)

                                                                          gtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAg                      <  1:1914964/100‑1 (MQ=255)

                                                                          gtgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAg                      <  1:2053032/100‑1 (MQ=255)

                                                                           tgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGc                     <  2:3608766/100‑1 (MQ=255)

                                                                           tgCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGc                     <  1:703744/100‑1 (MQ=255)

                                                                            gCCTTGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCg                    >  2:1463047/1‑100 (MQ=255)

                                                                               ttGTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAg                 <  2:3218486/100‑1 (MQ=255)

                                                                                 gTTCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGt               >  1:3796437/1‑100 (MQ=255)

                                                                                   tCTGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGttt             >  1:3780352/1‑100 (MQ=255)

                                                                                    cTGGTTTCTTCGGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGGCTTTCTGGTTGGTCAGATCCTGAGCGAAGGtttt            >  2:2186057/1‑100 (MQ=255)

                                                                                     tGGTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTg           <  1:4154025/100‑1 (MQ=255)

                                                                                      ggTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGc          >  1:3753438/1‑100 (MQ=255)

                                                                                      ggTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGc          >  2:3760935/1‑100 (MQ=255)

                                                                                      ggTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTAGCCTTTCTGGTTGGTCAGATACTGAGCGAAGGTTTTGc          >  2:1375287/1‑100 (MQ=255)

                                                                                       gTTTCTTCTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCt         <  1:2444350/100‑1 (MQ=255)

                                                                                         ttcttcTGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTcc       <  1:1872635/100‑1 (MQ=255)

                                                                                          tcttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCa      <  1:18863/100‑1 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATATGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     <  2:2676629/100‑1 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     >  2:628263/1‑100 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     <  2:1814225/100‑1 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     <  2:1917716/100‑1 (MQ=255)

                                                                                           cttctGTTCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAg     >  1:1596705/1‑100 (MQ=255)

GGCTGGCGTAATCCAGGTCGTTCGCCTGTTTAGCCCCGGTTTTGTACGCCCAGTCGAAGAATTTCAGCACTTCTGTGCCTTGTTCTGGTTTCTTCTGATCTTTGTGGATCAGAATGAACGTGGTAGAGGTAATAGGCCATGCATCTTCGCCTTTCTGGTTGGTCAGATCCTGAGCGAAGGTTTTGCTCCAGTCT  >  NC_000913/3910565‑3910758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`