Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA99.4% 542.3 / ‑4.8 164C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (82/79);  total (84/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 165 total aligned reads displayed.

GATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162875‑163069
                                                                                                  |                                                                                                
   cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              <  2:1043999/100‑1 (MQ=255)
   cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              <  1:357334/100‑1 (MQ=255)
   cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              >  2:1946344/1‑100 (MQ=255)
    ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             >  1:3720179/1‑100 (MQ=255)
    ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGccc                                                                                             <  1:3422478/100‑1 (MQ=255)
    cgcccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                              <  2:1046495/97‑1 (MQ=255)
       ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAgg                                                                                          >  1:508404/1‑100 (MQ=255)
        cGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGa                                                                                         <  1:4134783/100‑1 (MQ=255)
         ggCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAg                                                                                        >  2:1600320/1‑100 (MQ=255)
             ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    >  1:3886771/1‑100 (MQ=255)
             ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                    >  1:2879784/1‑100 (MQ=255)
              cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtgt                                                                                   >  1:4055941/1‑100 (MQ=255)
               gCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGtgtg                                                                                  >  1:2397277/1‑100 (MQ=255)
                cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGATACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                 <  2:476785/100‑1 (MQ=255)
                cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                 <  2:252042/100‑1 (MQ=255)
                 aaGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                                <  2:3851120/100‑1 (MQ=255)
                     gATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCGTTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGt                                                                            >  2:2862524/1‑100 (MQ=255)
                     gATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGt                                                                            >  2:678954/1‑100 (MQ=255)
                        gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         <  2:512928/100‑1 (MQ=255)
                        gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttn                                                                         <  1:307794/100‑2 (MQ=255)
                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                         >  1:2055988/1‑99 (MQ=255)
                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTg                                                                        >  1:2562787/1‑100 (MQ=255)
                         cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGCAGCGTGTGGCGCGATTTg                                                                        >  2:3549850/1‑100 (MQ=255)
                           cAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                                      <  1:3317267/100‑1 (MQ=255)
                            aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                     <  1:4081961/100‑1 (MQ=255)
                              aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                   <  1:2612313/100‑1 (MQ=255)
                                tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                 <  2:2414174/100‑1 (MQ=255)
                                tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                 <  1:1406919/100‑1 (MQ=255)
                                tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                 <  1:3951780/100‑1 (MQ=255)
                                  gtgCTGGCGACCAATATTGCTGAAACCAGTTTAGCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCTCTAGCGTGTGGCGCGTTTTGATCCtcgc                                                                >  2:2499730/1‑99 (MQ=255)
                                  gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                               >  2:303622/1‑100 (MQ=255)
                                  gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                               >  1:9904/1‑100 (MQ=255)
                                  gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                               >  1:3422867/1‑100 (MQ=255)
                                   tgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCAc                                                              >  1:876049/1‑100 (MQ=255)
                                       ggCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgggg                                                          <  2:1761676/100‑1 (MQ=255)
                                        gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  1:244503/100‑1 (MQ=255)
                                        gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                         <  2:3714106/100‑1 (MQ=255)
                                         cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                        <  2:808911/100‑1 (MQ=255)
                                         cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                        >  1:586904/1‑100 (MQ=255)
                                          gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                       >  2:684681/1‑100 (MQ=255)
                                           aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                      <  1:3097439/100‑1 (MQ=255)
                                           aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                      <  1:4131137/100‑1 (MQ=255)
                                              aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgc                                                   >  2:1752745/1‑100 (MQ=255)
                                               atatTTCTGAAAGCACTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCTTTTTGATCCGCGCACGGGGCTTAcgcg                                                  <  2:3782391/100‑1 (MQ=255)
                                               atatTGCTGAAACCAGTTTAACCATTGAAAGTATTCGTCTGGTGGTGGATTATGCCCAGGACAGTTTGGAGCGTTTTGCTCCGCGCACcggtcctagccg                                                  >  2:3415883/1‑91 (MQ=255)
                                                  ttGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACt                                               <  2:4144715/100‑1 (MQ=255)
                                                   tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                              <  2:3205356/100‑1 (MQ=255)
                                                    gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  2:3091434/1‑100 (MQ=255)
                                                    gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  1:482550/1‑100 (MQ=255)
                                                    gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  1:797750/1‑100 (MQ=255)
                                                    gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                             >  1:3951125/1‑100 (MQ=255)
                                                     cTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                            <  1:1031293/100‑1 (MQ=255)
                                                      tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                           <  2:1177121/100‑1 (MQ=255)
                                                       gAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                                          <  2:2103585/100‑1 (MQ=255)
                                                        aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                         <  1:2344400/100‑1 (MQ=255)
                                                        aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                         <  1:3273987/100‑1 (MQ=255)
                                                         aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                        >  2:1098270/1‑100 (MQ=255)
                                                         aaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACt                                        >  2:551015/1‑100 (MQ=255)
                                                           ccAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACGGATTACTCa                                      <  1:1395593/100‑1 (MQ=255)
                                                            cAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCaa                                     <  1:215303/100‑1 (MQ=255)
                                                              gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   <  2:131977/100‑1 (MQ=255)
                                                              gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                   >  1:1626432/1‑100 (MQ=255)
                                                                ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                 <  2:4060330/100‑1 (MQ=255)
                                                                 tcaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                                >  1:2807611/3‑100 (MQ=255)
                                                                  aaCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                               >  2:2239837/1‑100 (MQ=255)
                                                                   aCCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTa                              <  2:3143558/100‑1 (MQ=255)
                                                                   aCCATTGAAGGAAATCGTCTGCTAGTGGATTTTGCGCAGTAGCGTGTGGCGCGTTTTGATTCGCGCACGGGGCTTACGCGACTGATTACTCAACTCGtt                               >  2:443548/1‑99 (MQ=255)
                                                                   aCCATCGCAGGTATTCATATGGTGGTGGCTTATGCCCAGGAGGGTGTAAAACAAAAACAACCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTa                              <  2:3537047/100‑1 (MQ=255)
                                                                    ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                             <  1:3949860/100‑1 (MQ=255)
                                                                    ccATTGAAGGTATTCGTCTGGTGGTGGATTATACCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                             >  2:632302/1‑100 (MQ=255)
                                                                     cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTa                              >  2:3387768/1‑98 (MQ=255)
                                                                     cATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGc                            <  1:1916133/100‑1 (MQ=255)
                                                                      aTTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc                           >  2:2888542/1‑100 (MQ=255)
                                                                       ttGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc                           <  2:1208418/99‑1 (MQ=255)
                                                                         gcaGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        <  1:943207/98‑1 (MQ=255)
                                                                         gAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  1:2057479/1‑100 (MQ=255)
                                                                         gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        >  1:3390875/1‑100 (MQ=255)
                                                                         gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                        <  1:1133522/100‑1 (MQ=255)
                                                                           aGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGAGCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa                      >  2:2548574/1‑100 (MQ=255)
                                                                            ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt                     >  1:587928/1‑100 (MQ=255)
                                                                               aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                  >  2:824064/1‑100 (MQ=255)
                                                                               aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                  <  1:1263495/100‑1 (MQ=255)
                                                                                ttCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAt                 >  1:2542114/1‑100 (MQ=255)
                                                                                 tCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATg                <  1:1752755/100‑1 (MQ=255)
                                                                                   ttttggttgtgcTTTCTGCCCAGGAGCGTATGGCGCGTGGTGATCCGCGCCCGGGGCTTACGCGACTCATTGCCCGACCCGTTATGCAGGCATCCGTgcc              <  2:847524/97‑3 (MQ=255)
                                                                                   gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc              <  1:445744/100‑1 (MQ=255)
                                                                                      tggtggtggATTATGCCCAGGAGCGTGTTGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa           <  1:1035768/100‑1 (MQ=255)
                                                                                      nggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa           >  2:191278/2‑100 (MQ=255)
                                                                                       ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg          >  1:1602815/1‑100 (MQ=255)
                                                                                       ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg          >  2:972720/1‑100 (MQ=255)
                                                                                       ggtggcggATAATACCCAGGCGCGTGTGGCGCGTTTTGATGCGCGGACGTGGACTACGCGACTGATTACTCAACGAGTAAACCAGGCATCCATGAc              >  2:2682089/1‑96 (MQ=255)
                                                                                         tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        <  1:1126889/100‑1 (MQ=255)
                                                                                          ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGCTTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  1:841838/1‑100 (MQ=255)
                                                                                          ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg        >  2:2954504/1‑99 (MQ=255)
                                                                                          ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  2:2126415/1‑100 (MQ=255)
                                                                                          ggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGt       >  1:3686440/1‑100 (MQ=255)
                                                                                            tggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc     >  2:1502099/1‑100 (MQ=255)
                                                                                            tggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc     >  1:3197525/1‑100 (MQ=255)
                                                                                             ggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGcc    >  2:1880468/1‑100 (MQ=255)
                                                                                               aTTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCgg  >  1:4114431/1‑100 (MQ=255)
                                                                                                  |                                                                                                
GATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGG  >  NC_000913/162875‑163069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: