Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,721 G→T 83.3% C51* (TGC→TGA ybcJ ← ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,7210GT83.3% 383.4 / 70.0 161C51* (TGC→TGAybcJribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base T (76/58);  total (91/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 166 total aligned reads displayed.

AAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTC  >  NC_000913/556622‑556820
                                                                                                   |                                                                                                   
aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTg                                                                                                     <  1:3227059/100‑1 (MQ=255)
  aaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCa                                                                                                   <  1:2638231/100‑1 (MQ=255)
      gaACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTCTTCACCGt                                                                                               >  2:1368569/1‑100 (MQ=255)
      gaACCGGCCTGTTGATACAGGCCGGGAAAGGGAGCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGt                                                                                               >  2:2503356/1‑100 (MQ=255)
        aCCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGttt                                                                                             >  1:230665/1‑100 (MQ=255)
        aCCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGttt                                                                                             >  1:2576776/1‑100 (MQ=255)
         ccGGCCTGTATCAACAGGCCGGGAAAGGGATCCGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTg                                                                                            >  2:203121/1‑100 (MQ=255)
           ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcg                                                                                          >  2:390682/1‑100 (MQ=255)
            gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTgcgc                                                                                         >  1:176527/1‑100 (MQ=255)
            gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTgcgc                                                                                         <  2:4010708/100‑1 (MQ=255)
             ccTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGcgcg                                                                                        >  1:1717091/1‑100 (MQ=255)
               tGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGtt                                                                                      <  2:1419384/100‑1 (MQ=255)
                gTTGATACGGGCCGGGAAAGGGATCAGGCAACAAGCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     <  2:1154456/100‑1 (MQ=255)
                gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     <  2:218965/100‑1 (MQ=255)
                gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     <  1:1680064/100‑1 (MQ=255)
                gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     >  1:2312563/1‑100 (MQ=255)
                 ttGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGttt                                                                                     <  2:3593755/99‑1 (MQ=255)
                 ttGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTc                                                                                    >  2:3983523/1‑100 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCaa                                                                                  >  1:3032759/1‑100 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCaa                                                                                  >  2:3197617/1‑100 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCa                                                                                   >  2:1104392/1‑99 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCaa                                                                                  >  2:2375116/1‑100 (MQ=255)
                   gATACAGGCCGGGAAAGGGATCAGGCAACAACATGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTTAGCTTTTGTGCGTTTc                                                                                    >  2:2720648/1‑98 (MQ=255)
                     tACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAAcc                                                                                <  2:3578442/100‑1 (MQ=255)
                       cAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGc                                                                              >  2:3608718/1‑100 (MQ=255)
                       cAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGc                                                                              >  1:3694792/1‑100 (MQ=255)
                        aGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCa                                                                             >  2:1280782/1‑100 (MQ=255)
                        aGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCa                                                                             >  1:2156525/1‑100 (MQ=255)
                         ggCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCa                                                                             >  2:3358282/1‑99 (MQ=255)
                         ggCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCAc                                                                            >  1:3021320/1‑100 (MQ=255)
                         ggCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCAc                                                                            >  2:144693/1‑100 (MQ=255)
                         ggCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCAc                                                                            >  1:341771/1‑100 (MQ=255)
                            cGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGt                                                                         >  1:2754261/1‑100 (MQ=255)
                                  aGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACtt                                                                   >  1:3384524/1‑100 (MQ=255)
                                  aGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATGTTTCAGCGTTTGCGTGTTTCAACCGCACCGTCGACtt                                                                   >  2:2807249/1‑100 (MQ=255)
                                   nggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:1348233/2‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:2468892/1‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:3860143/1‑100 (MQ=255)
                                   gggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                                                  >  1:3755530/1‑100 (MQ=255)
                                    ggATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTc                                                                 >  2:773452/1‑100 (MQ=255)
                                     gATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                                                >  2:322718/1‑100 (MQ=255)
                                     gATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                                                >  2:3316734/1‑100 (MQ=255)
                                     gATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTCACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTc                                                                 <  1:1218702/99‑1 (MQ=255)
                                      aTCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                                                               >  1:3716638/1‑100 (MQ=255)
                                      aTCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                                                               >  1:737052/1‑100 (MQ=255)
                                       tCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAcc                                                              <  2:2070051/100‑1 (MQ=255)
                                        cAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCt                                                             <  2:2900858/100‑1 (MQ=255)
                                         aGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCATCTg                                                            >  2:1542350/1‑100 (MQ=255)
                                          ggCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTgg                                                           >  2:2131598/1‑100 (MQ=255)
                                          ggCAACAACCTGTACGCTGTGACCTGCAAAAATCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTgg                                                           >  2:1806880/1‑100 (MQ=255)
                                                 acCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCgg                                                    >  1:2045884/1‑100 (MQ=255)
                                                 acCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCgg                                                    <  2:4063453/100‑1 (MQ=255)
                                                   cTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCa                                                  <  1:3159100/100‑1 (MQ=255)
                                                    tGTACGCTGTGACCTGCACAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCaa                                                 >  1:1866198/1‑100 (MQ=255)
                                                            gtgACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAtt                                         >  1:1828763/1‑100 (MQ=255)
                                                             tgACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt                                        <  1:3614222/100‑1 (MQ=255)
                                                              gACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAtttt                                       >  1:3266977/1‑100 (MQ=255)
                                                                ccTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCTCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCg                                     <  1:1883433/100‑1 (MQ=255)
                                                                ccTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCg                                     <  2:3179553/100‑1 (MQ=255)
                                                                 cTGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc                                    >  1:679704/1‑100 (MQ=255)
                                                                  tGCAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc                                   >  1:3569569/1‑100 (MQ=255)
                                                                  tGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc                                   >  2:4011439/1‑100 (MQ=255)
                                                                    cAAAACTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTg                                 <  2:3676036/100‑1 (MQ=255)
                                                                     aaaaCTCACTGTCTGACCGGCGACGATTTTTTTTTTTTTTTTCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc                                >  2:2718187/1‑100 (MQ=255)
                                                                     aaaaCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc                                >  2:1632439/1‑100 (MQ=255)
                                                                     aaaaCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACATTAACATGGCCTTCGGCAATCGCGATTTTCGCCTgc                                >  2:1505494/1‑100 (MQ=255)
                                                                      aaaCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcg                               >  1:1062211/1‑100 (MQ=255)
                                                                        aCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcg                             <  1:2382934/100‑1 (MQ=255)
                                                                        aCTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcg                             >  1:2517706/1‑100 (MQ=255)
                                                                         cTCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcgcgc                            >  1:1560499/1‑100 (MQ=255)
                                                                          tCACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgcc                           <  1:458889/100‑1 (MQ=255)
                                                                           cACTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgccg                          >  1:1204772/1‑100 (MQ=255)
                                                                            aCTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgccgc                         <  1:1464997/100‑1 (MQ=255)
                                                                             cTGTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGcgccgc                         >  2:2600439/1‑99 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCttt                      >  1:992385/1‑100 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCttt                      >  1:4088289/1‑100 (MQ=255)
                                                                               gTCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACATGGCCTTCGGCAATCGCGATTTTCGCATGCGCGCCGCttt                      >  2:849967/1‑100 (MQ=255)
                                                                                tCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTc                     <  2:1881559/100‑1 (MQ=255)
                                                                                tCTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTc                     <  2:566998/100‑1 (MQ=255)
                                                                                 cTGACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCg                    >  1:4017181/1‑100 (MQ=255)
                                                                                 cTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCg                    >  2:926916/1‑100 (MQ=255)
                                                                                 cTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCg                    >  2:232453/1‑100 (MQ=255)
                                                                                 cTGACCGGCCACGATTTTTCAGCGTTTCCGCGTTTCAACCGCTCCGGCGCCTTTCACCTGGCCTTCGGCAATTGCGATTCTCGCCTGCGCGCCGCTTTCg                    <  2:1340594/100‑1 (MQ=255)
                                                                                  tGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTc                     <  2:2489579/98‑1 (MQ=255)
                                                                                   gACCGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCt                  <  1:2274440/100‑1 (MQ=255)
                                                                                     ccGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTcc                >  2:1701555/1‑100 (MQ=255)
                                                                                     ccGGCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCACTcc                >  1:3562402/1‑100 (MQ=255)
                                                                                     ccGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTcc                >  1:727015/1‑100 (MQ=255)
                                                                                     ccGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTcc                <  2:4138376/100‑1 (MQ=255)
                                                                                       ggCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAg              >  1:460289/1‑100 (MQ=255)
                                                                                       ggCGACGATTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAg              <  1:2545800/100‑1 (MQ=255)
                                                                                            cgaTTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTc         >  2:3014012/1‑100 (MQ=255)
                                                                                            cgaTTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTc         <  1:2101228/100‑1 (MQ=255)
                                                                                            cgaTTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTc         <  1:3343079/100‑1 (MQ=255)
                                                                                            cgaTTTTTCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTCCGCTCCAGCCTTc         <  2:3860114/100‑1 (MQ=255)
                                                                                              aTTTTGCAGCGTTTTCGCGTTTCAACCGCACCGTTGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTGCGCTCCAGCCTTcc        <  2:1827170/99‑1 (MQ=255)
                                                                                               ttttGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCTTGCGCGCCGCTTTCGCTCCAGCCTTCCAg      <  2:2869403/100‑1 (MQ=255)
                                                                                               ttttGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAg      <  2:4128392/100‑1 (MQ=255)
                                                                                                   gCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTc  <  1:3535289/100‑1 (MQ=255)
                                                                                                   gCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTc  >  1:1080247/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTC  >  NC_000913/556622‑556820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: