Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,631,700 T→C 100% L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,7000TC100.0% 649.9 / NA 181L323P (CTT→CCT) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (82/99);  total (82/99)

Only 100 of 181 total aligned reads displayed.

GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATT  >  NC_000913/4631603‑4631795
                                                                                                 |                                                                                               
ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                               <  1:3406706/100‑1 (MQ=255)
ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                               <  2:3271329/100‑1 (MQ=255)
 gCAACGATGTCACCGACGAGTAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                              >  2:2555137/1‑100 (MQ=255)
 gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                              <  1:2492110/100‑1 (MQ=255)
 gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                              <  1:708865/100‑1 (MQ=255)
 gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                              >  2:3918140/1‑100 (MQ=255)
  cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                             >  1:1047127/1‑100 (MQ=255)
  cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                             <  2:1088224/100‑1 (MQ=255)
  cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                             <  1:1583203/100‑1 (MQ=255)
     cGATGTCACCGACGAGCAGGCGAAATTGCGGGATTACGCCATTATGCGCTCGCAAGCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTgg                                                                                              <  2:491450/96‑1 (MQ=255)
        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGGACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                       <  1:2302838/100‑1 (MQ=255)
        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                       >  1:2621150/1‑100 (MQ=255)
        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                       <  1:4120097/100‑1 (MQ=255)
         gTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                      >  1:2876671/1‑100 (MQ=255)
         gTCACCGACGAGAAGGAGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCGTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                      >  2:2480701/1‑100 (MQ=255)
          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCg                                                                                      >  2:1291628/1‑99 (MQ=255)
          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa                                                                                     >  1:3814923/1‑100 (MQ=255)
           cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                    <  2:1312783/100‑1 (MQ=255)
           cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                    >  2:223553/1‑100 (MQ=255)
           cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                    <  1:3573191/100‑1 (MQ=255)
             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                  >  2:3977944/1‑100 (MQ=255)
              cgacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcg                                                                                  <  1:3508446/99‑1 (MQ=255)
                      aGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                         >  1:2782446/1‑100 (MQ=255)
                      aGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                         >  2:3829212/1‑100 (MQ=255)
                      aGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCt                                                                         <  1:763049/100‑1 (MQ=255)
                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                        >  2:3347707/1‑100 (MQ=255)
                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                        <  2:4144850/100‑1 (MQ=255)
                         cGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGaa                                                                      >  1:175859/1‑100 (MQ=255)
                          gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                     <  1:1919433/100‑1 (MQ=255)
                               ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                                <  1:2785957/100‑1 (MQ=255)
                                gcgcGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctgg                                                               <  2:1217907/100‑1 (MQ=255)
                                 cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggc                                                              <  2:1059327/100‑1 (MQ=255)
                                  gcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggct                                                             >  1:1411552/1‑100 (MQ=255)
                                  gcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggct                                                             >  2:2208536/1‑100 (MQ=255)
                                   cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctg                                                            >  1:3011402/1‑100 (MQ=255)
                                   cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctggctg                                                            >  2:2824874/1‑100 (MQ=255)
                                     aTGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCtggctggc                                                          >  1:1202073/1‑100 (MQ=255)
                                     aTGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCtggccggc                                                          <  1:25027/100‑1 (MQ=255)
                                      tGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGgcg                                                         >  1:1426064/1‑100 (MQ=255)
                                        aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                       <  1:2560955/100‑1 (MQ=255)
                                         cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                                      >  2:660632/1‑100 (MQ=255)
                                          gCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTc                                                     <  1:1730123/100‑1 (MQ=255)
                                           ccATTATGCGCTCGCAATCTACTTCGCTTATTGACCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                    <  1:531352/100‑1 (MQ=255)
                                           ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                    <  2:2946452/100‑1 (MQ=255)
                                           ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                                    <  1:2365165/100‑1 (MQ=255)
                                              ttacccgcTCGCAATCTACCTCGCTTATTGAGCGCCGTCTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                   <  2:3398738/93‑1 (MQ=255)
                                                 tGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTAt                                              >  2:2165536/1‑100 (MQ=255)
                                                  gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                             <  2:846622/100‑1 (MQ=255)
                                                  gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                             <  2:2692340/100‑1 (MQ=255)
                                                  gcgcTCGCAATCTACTTCGCTTATTGAACGCCCTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                             >  1:3953043/1‑100 (MQ=255)
                                                   cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                            >  1:265869/1‑100 (MQ=255)
                                                    gcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                           >  2:912420/1‑100 (MQ=255)
                                                    gcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGa                                           >  2:3004772/1‑100 (MQ=255)
                                                     cTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGaa                                          <  2:2295644/100‑1 (MQ=255)
                                                      tCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAg                                         <  1:1481457/100‑1 (MQ=255)
                                                       cGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGc                                        >  2:3105013/1‑100 (MQ=255)
                                                        gCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATTGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg                                       >  1:2282668/1‑100 (MQ=255)
                                                        gCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg                                       >  1:1217916/1‑100 (MQ=255)
                                                        gCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg                                       <  2:1229986/100‑1 (MQ=255)
                                                         cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCTATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga                                      <  2:4083629/100‑1 (MQ=255)
                                                         cAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga                                      >  2:337585/1‑100 (MQ=255)
                                                         cAATCTACTTCGCTCATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCga                                      <  1:565411/100‑1 (MQ=255)
                                                          aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                     <  2:3899480/100‑1 (MQ=255)
                                                            tCTGCTTCGCTTATTGAACGCCGTGTACGAATGGTGCCTGGCACCGGGGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                                   <  1:1446675/100‑1 (MQ=255)
                                                            tCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                                   <  2:3355186/100‑1 (MQ=255)
                                                             cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga                                  <  2:1402636/100‑1 (MQ=255)
                                                              tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                 >  1:4137850/1‑100 (MQ=255)
                                                              tACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagag                                 >  2:2136475/1‑100 (MQ=255)
                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTTGCACCGTCGATCGTCGCGGCCTGAATACCTGGCTGGCACGTCTGCCTATGGAAGCgaaagaga                                >  2:3653956/1‑99 (MQ=255)
                                                                  tCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaag                             <  2:2523373/100‑1 (MQ=255)
                                                                  tCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaag                             <  1:4081627/100‑1 (MQ=255)
                                                                   cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaaga                            <  2:1568893/100‑1 (MQ=255)
                                                                    gCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                           >  1:1366069/1‑100 (MQ=255)
                                                                    gCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                           >  1:3194188/1‑100 (MQ=255)
                                                                    gCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                           >  2:1899339/1‑100 (MQ=255)
                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                          <  2:67296/100‑1 (MQ=255)
                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                          <  2:1615573/100‑1 (MQ=255)
                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCAAAAGAGAAAGATg                          <  2:1174279/100‑1 (MQ=255)
                                                                        aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGGAGCGAAAGAGAAAGATGAAt                       >  2:211004/1‑100 (MQ=255)
                                                                        aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAt                       >  2:550828/1‑100 (MQ=255)
                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAGGATGAATGGc                    >  2:2320464/1‑100 (MQ=255)
                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                    >  1:1627649/1‑100 (MQ=255)
                                                                            aaCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCg                   <  1:2082954/100‑1 (MQ=255)
                                                                             aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt                  <  1:213692/100‑1 (MQ=255)
                                                                               gCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTa                <  1:505329/100‑1 (MQ=255)
                                                                                  gtgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAGGAATGGCGTTACt              >  1:2268135/1‑99 (MQ=255)
                                                                                    gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc           <  1:3177654/100‑1 (MQ=255)
                                                                                     tACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCa          <  1:1286999/100‑1 (MQ=255)
                                                                                      aCGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAg         <  2:782558/100‑1 (MQ=255)
                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGCTGAATGGCGTTACTGGCAgg        <  2:638168/100‑1 (MQ=255)
                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg        >  1:3753786/1‑100 (MQ=255)
                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAACACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAgg        <  2:2268427/100‑1 (MQ=255)
                                                                                        gAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGc       <  2:1664802/100‑1 (MQ=255)
                                                                                         aaTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCg      <  2:758516/100‑1 (MQ=255)
                                                                                          aTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCgg     <  1:1561834/100‑1 (MQ=255)
                                                                                          aTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCgg     <  2:1413120/100‑1 (MQ=255)
                                                                                           tGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGa    <  2:726724/100‑1 (MQ=255)
                                                                                            ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAGGCGAAAGAGAAAGATGAATGGCGTGACTGGCAGGCgg     >  2:4171569/1‑98 (MQ=255)
                                                                                            ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAt   <  1:3128157/100‑1 (MQ=255)
                                                                                             gcgcCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACAGGCAGGCGGAtt  >  2:3573050/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATT  >  NC_000913/4631603‑4631795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑