Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 723,744 C→G 100% R224P (CGC→CCC)  kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913723,7440CG75.1% 283.7 / 102.0 144R224P (CGC→CCC) kdpDfused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base G (52/56);  total (70/74)
Fisher's exact test for biased strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTT  >  NC_000913/723647‑723840
                                                                                                 |                                                                                                
gCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGgggc                                                                                                <  2:1525853/100‑1 (MQ=255)
  aTAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCACGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAg                                                                                              >  2:940726/1‑100 (MQ=255)
   tAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGt                                                                                             >  2:1770375/1‑100 (MQ=255)
   tAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGt                                                                                             >  1:3837104/1‑100 (MQ=255)
   tAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGt                                                                                             >  1:558170/1‑100 (MQ=255)
    aaaaGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTg                                                                                            <  1:2462764/100‑1 (MQ=255)
      aaGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGcc                                                                                          <  1:3779955/100‑1 (MQ=255)
      aaGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGcc                                                                                          <  1:2018619/100‑1 (MQ=255)
      aaGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGcc                                                                                          <  1:1121151/100‑1 (MQ=255)
        ggATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAg                                                                                        <  1:421086/100‑1 (MQ=255)
        ggATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAg                                                                                        <  1:644323/100‑1 (MQ=255)
        ggATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAg                                                                                        <  2:3124758/100‑1 (MQ=255)
        ggATCGCGTCGCGCGTGTGCCACACTTTCTCTTAGCCAGGATGCCCACGCCAGGCGCGCATTTGCTTATCAACGCGATCGGCAGTACGGGGCAGTGCCAg                                                                                        >  2:814955/1‑100 (MQ=255)
            cgcgtcgcgCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTcg                                                                                    <  1:3572454/100‑1 (MQ=255)
             gcgtcgcgCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTcgc                                                                                   <  1:4129031/100‑1 (MQ=255)
             gcgtcgcgCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTcgc                                                                                   <  1:2703518/100‑1 (MQ=255)
              cgtcgcgCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTcgcg                                                                                  <  1:2728079/100‑1 (MQ=255)
                 cgcgcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAg                                                                               <  1:3675/100‑1 (MQ=255)
                  gcgcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAgg                                                                              >  2:1184710/1‑100 (MQ=255)
                   cgcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAggg                                                                             >  1:642731/1‑100 (MQ=255)
                   cgcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGCGGCAGTGCCAGTTCGCGCAggg                                                                             >  2:906853/1‑100 (MQ=255)
                    gcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGc                                                                            <  2:4144195/100‑1 (MQ=255)
                    gcgTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGc                                                                            <  2:3994630/100‑1 (MQ=255)
                       tgtgCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGAt                                                                         >  2:3069920/1‑100 (MQ=255)
                        gtgCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATc                                                                        >  1:2989858/1‑100 (MQ=255)
                          gCCACACTTTCTCTTCGCTAGGATGCCCCGGCCAGGCGCGCATTTGTTTATAAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAg                                                                      >  2:1300497/1‑100 (MQ=255)
                          gCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAg                                                                      <  2:477576/100‑1 (MQ=255)
                          gCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAg                                                                      >  1:2991346/1‑100 (MQ=255)
                           ccACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGa                                                                     >  2:2876807/1‑100 (MQ=255)
                            cacaCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGAt                                                                    >  2:1033021/1‑100 (MQ=255)
                            cacaCTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGAt                                                                    >  2:584601/1‑100 (MQ=255)
                              cacTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTa                                                                  <  2:1654395/100‑1 (MQ=255)
                              cacTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTa                                                                  >  1:991345/1‑100 (MQ=255)
                                cTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTAcc                                                                <  2:2846817/100‑1 (MQ=255)
                                cTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTAcc                                                                >  1:3260069/1‑100 (MQ=255)
                                 tttCTCTTCGCCAGGCTGCCCCCGCCAGGCGCGCATGTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCt                                                               <  2:1971520/100‑1 (MQ=255)
                                 tttCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCt                                                               <  2:688789/100‑1 (MQ=255)
                                  ttCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCtt                                                              <  1:2364079/100‑1 (MQ=255)
                                   tctcTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCttt                                                             >  1:2977754/1‑100 (MQ=255)
                                    ctctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTg                                                            <  2:662003/100‑1 (MQ=255)
                                    ctctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTg                                                            <  2:3715700/100‑1 (MQ=255)
                                      ctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          <  1:2232621/100‑1 (MQ=255)
                                      ctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          <  1:3150993/100‑1 (MQ=255)
                                      ctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          <  1:3304111/100‑1 (MQ=255)
                                      ctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          >  1:3420294/1‑100 (MQ=255)
                                      ctTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          >  1:1878231/1‑100 (MQ=255)
                                       ttCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCgg                                                         <  2:2346153/100‑1 (MQ=255)
                                         cGCCAGGACGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGTTCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCg                                                          <  1:1583094/97‑1 (MQ=255)
                                             aGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGaaaaaa                                                   <  2:3260046/100‑1 (MQ=255)
                                              ggATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAAt                                                  >  2:1327047/1‑100 (MQ=255)
                                               gATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATg                                                 <  2:40095/100‑1 (MQ=255)
                                               gATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATg                                                 <  2:3205809/100‑1 (MQ=255)
                                                  gctagcggcAGGCGCGGATTGGGTCATCAACGCAAGCGACAGTATCGCGCAGTGCAAGTTCGCGCAGGTCGATAAGATGGCCTGTGCGGAAAAAATGt                                                <  2:2062727/90‑1 (MQ=255)
                                                   cccccGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGGAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCa                                             >  1:2925567/1‑100 (MQ=255)
                                                   cccccGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCa                                             >  2:1815486/1‑100 (MQ=255)
                                                   cccccGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCa                                             >  1:3860808/1‑100 (MQ=255)
                                                    ccccGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCaa                                            >  2:1262702/1‑100 (MQ=255)
                                                       cGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATgg                                         <  2:537018/100‑1 (MQ=255)
                                                       cGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATgg                                         <  2:3372016/100‑1 (MQ=255)
                                                       cGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTAAATAAGAAAAAAAAATGTTCAATgg                                         <  2:745404/100‑1 (MQ=255)
                                                        gCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGgc                                        <  1:873872/100‑1 (MQ=255)
                                                              cgcgcATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTc                                  <  2:138456/100‑1 (MQ=255)
                                                               gcgcATTTGCCCAACAACGCGATCGGCTGTACGGCGCAGTGCCAGTTCTCGCAGGGCGATCAGTTTACCTTTGCTGAAAAAATGTTCAATGGCGCGCTcc                                 <  2:4158646/100‑1 (MQ=255)
                                                                  cATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGcc                              <  1:1262709/100‑1 (MQ=255)
                                                                  cATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGcc                              <  1:1182509/100‑1 (MQ=255)
                                                                  cATTTGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTAAAAAAAAAAAAAAAATGTTCAATGGCGCGCTCCGcc                              <  2:3101975/100‑1 (MQ=255)
                                                                    tctGCTCATCAGCGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAACAAATGTTCAATGGCGCGCTCCGCCTg                            <  2:3415291/98‑1 (MQ=255)
                                                                      tGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGcc                          <  2:1197177/100‑1 (MQ=255)
                                                                      tGCTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGcc                          >  1:156632/1‑100 (MQ=255)
                                                                      tGCTCATCAACGCGAGCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGcc                              <  2:4085947/96‑1 (MQ=255)
                                                                        cTCATCAACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCATGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATTGCGCGCTCCGCCTGCCCg                        >  2:1384491/1‑100 (MQ=255)
                                                                          catcaACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGCTCAATGGCGCGCTCCGCCTGCCCGGc                      <  1:2539426/100‑1 (MQ=255)
                                                                           atcaACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCa                     <  1:4011588/100‑1 (MQ=255)
                                                                           atcaACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGTTCCGCCTGCCCGGCa                     >  1:1398154/1‑100 (MQ=255)
                                                                            tcaACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCaa                    <  1:2177844/100‑1 (MQ=255)
                                                                            tcaACGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCaa                    >  1:1705517/1‑100 (MQ=255)
                                                                              aaCGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCACTGGCGCGCTCCGCCTGCCCGGCAata                  >  1:2136075/1‑100 (MQ=255)
                                                                              aaCGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAata                  >  1:1261049/1‑100 (MQ=255)
                                                                              aaCGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAata                  >  1:2284996/1‑100 (MQ=255)
                                                                              aaCGCGATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAat                   <  2:2732273/99‑1 (MQ=255)
                                                                              aaCGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAata                  >  2:4091116/1‑100 (MQ=255)
                                                                                cgcgATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAatat                 <  1:1021506/99‑1 (MQ=255)
                                                                                cgcgATCGGCAGTACGGCGCAGTGCCAGTTCGCTCAGGGCGATCAGAGTACCGTTGCGGAAAAAATGGTCAATGGCGCGCTCCGCCTGCCCGGCAAtata                >  2:835489/1‑100 (MQ=255)
                                                                                  cgATCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAg               <  1:688950/99‑1 (MQ=255)
                                                                                    aTCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCATTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACt            <  2:2559746/100‑1 (MQ=255)
                                                                                    aTCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACt            >  1:1398231/1‑100 (MQ=255)
                                                                                     tCGGCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCTATCAGATAAAAAAAAAAAAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACt            <  2:2248375/99‑1 (MQ=255)
                                                                                       ggCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTg         >  1:3058165/1‑100 (MQ=255)
                                                                                       ggCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTg         >  2:2915074/1‑100 (MQ=255)
                                                                                       ggCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAAGATAGACTTTg         >  2:2746365/1‑100 (MQ=255)
                                                                                       ggCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTg         >  1:3108270/1‑100 (MQ=255)
                                                                                       gccAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTg         >  1:2831131/3‑100 (MQ=255)
                                                                                        gCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGc        >  2:3609612/1‑100 (MQ=255)
                                                                                        gCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGc        <  1:4038438/100‑1 (MQ=255)
                                                                                        gCAGTACGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGc        >  2:3022528/1‑100 (MQ=255)
                                                                                            tACGGGGCAGTGCCAGTACGCGCAGGGAGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTTCGCGGCAATATAGACTTTGCct      <  2:2545381/98‑1 (MQ=255)
                                                                                            tACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCcttc    <  2:2069912/100‑1 (MQ=255)
                                                                                              cGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCcttctt  <  1:2511087/100‑1 (MQ=255)
                                                                                              cGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCcttctt  >  2:38978/1‑100 (MQ=255)
                                                                                              cGGGGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCcttctt  <  2:942073/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GCATAAAAGGATCGCGTCGCGCGTGTGCCACACTTTCTCTTCGCCAGGATGCCCCCGCCAGGCGCGCATTTGCTCATCAACGCGATCGGCAGTACGGCGCAGTGCCAGTTCGCGCAGGGCGATCAGATTACCTTTGCGGAAAAAATGTTCAATGGCGCGCTCCGCCTGCCCGGCAATATAGACTTTGCCTTCTT  >  NC_000913/723647‑723840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑