Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,886 T→A 15.3% L327Q (CTG→CAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,8860TA15.3% 418.1 / 60.5 164L327Q (CTG→CAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (61/77);  new base A (11/14);  total (72/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 168 total aligned reads displayed.

TGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAT  >  NC_000913/756787‑756985
                                                                                                   |                                                                                                   
tGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCt                                                                                                     <  2:1841730/100‑1 (MQ=255)
 gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTg                                                                                                    <  2:3304824/100‑1 (MQ=255)
 gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTg                                                                                                    >  1:1237106/1‑100 (MQ=255)
 gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTg                                                                                                    >  2:1659897/1‑100 (MQ=255)
  aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGc                                                                                                   >  1:1183304/1‑100 (MQ=255)
  aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGc                                                                                                   >  1:3916441/1‑100 (MQ=255)
   tCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGcc                                                                                                  >  2:3881450/1‑100 (MQ=255)
    cGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCg                                                                                                 >  2:3803533/1‑100 (MQ=255)
     gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg                                                                                                <  2:3471003/100‑1 (MQ=255)
     gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg                                                                                                <  1:2435880/100‑1 (MQ=255)
     gAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg                                                                                                >  1:857898/1‑100 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCggg                                                                                               <  2:2272986/100‑1 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCggg                                                                                               <  1:883994/100‑1 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg                                                                                               <  1:376716/100‑1 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg                                                                                               <  2:3040842/100‑1 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg                                                                                               <  2:3150001/100‑1 (MQ=255)
      aaaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCggg                                                                                               <  1:2698074/100‑1 (MQ=255)
       aaTCCGTGAAGGTCGCGGCTGCGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCAGGGt                                                                                              >  2:1807238/1‑100 (MQ=255)
        aTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTa                                                                                             <  2:2384048/100‑1 (MQ=255)
          ccGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATc                                                                                           >  2:10572/1‑100 (MQ=255)
             tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTg                                                                                        <  2:84512/100‑1 (MQ=255)
             tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTg                                                                                        >  1:4075980/1‑100 (MQ=255)
              gAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTgg                                                                                       <  1:3293628/100‑1 (MQ=255)
                aGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAg                                                                                     >  1:3041710/1‑100 (MQ=255)
                 ggTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGc                                                                                    <  2:356798/100‑1 (MQ=255)
                    cgcgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCttt                                                                                 >  1:1688309/1‑100 (MQ=255)
                     gcgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTc                                                                                <  1:807001/100‑1 (MQ=255)
                      cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTcc                                                                               <  1:2899431/100‑1 (MQ=255)
                      cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTcc                                                                               <  2:3781350/100‑1 (MQ=255)
                         cTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGt                                                                            <  1:633813/100‑1 (MQ=255)
                           gggATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTAc                                                                          <  2:2526376/98‑1 (MQ=255)
                            tgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTAcc                                                                         <  1:2378726/100‑1 (MQ=255)
                            tgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTAcc                                                                         <  2:2124777/100‑1 (MQ=255)
                            tgATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTAc                                                                          >  1:1997380/1‑99 (MQ=255)
                             gATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCt                                                                        >  1:533572/1‑100 (MQ=255)
                             gATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCt                                                                        <  1:2091679/100‑1 (MQ=255)
                               tGGTCCGTGGGGGCCACACGCGAAACTGAATCTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTc                                                                      <  2:449967/100‑1 (MQ=255)
                               tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTc                                                                      >  1:3054154/1‑100 (MQ=255)
                               tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTc                                                                      >  1:1602074/1‑100 (MQ=255)
                                ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg                                                                     >  1:2162446/1‑100 (MQ=255)
                                 gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                    >  1:2878994/1‑100 (MQ=255)
                                 gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                    >  2:4091943/1‑100 (MQ=255)
                                 gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                    <  2:1967572/100‑1 (MQ=255)
                                   ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTc                                                                  <  1:2764223/100‑1 (MQ=255)
                                    cGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCa                                                                 <  2:1278545/100‑1 (MQ=255)
                                       gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt                                                              <  2:3145006/100‑1 (MQ=255)
                                        ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTc                                                             <  2:4115533/100‑1 (MQ=255)
                                        ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTc                                                             >  2:1494257/1‑100 (MQ=255)
                                         gggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCg                                                            >  2:569924/1‑100 (MQ=255)
                                            ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                         >  1:3094589/1‑100 (MQ=255)
                                            ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                         >  1:2253706/1‑100 (MQ=255)
                                            ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                         >  2:3232986/1‑100 (MQ=255)
                                             cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc                                                        <  1:1069882/100‑1 (MQ=255)
                                             cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc                                                        >  2:2942144/1‑100 (MQ=255)
                                              acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                       >  1:4118349/1‑100 (MQ=255)
                                                  gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGa                                                   <  1:1555578/100‑1 (MQ=255)
                                                   cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                                  >  2:898893/1‑100 (MQ=255)
                                                     aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                                <  1:2057051/100‑1 (MQ=255)
                                                     aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                                <  1:2337772/100‑1 (MQ=255)
                                                       actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAagag                                              <  1:2100297/100‑1 (MQ=255)
                                                        ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGc                                             >  2:1401089/1‑100 (MQ=255)
                                                         tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTTGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGATcc                                            <  2:181428/100‑1 (MQ=255)
                                                         tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                            >  1:200321/1‑100 (MQ=255)
                                                         tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                            >  1:2225593/1‑100 (MQ=255)
                                                           aaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGa                                          <  1:261075/100‑1 (MQ=255)
                                                               tCGATCACCTGGGTAAAGAAGTTCTCGAACCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTcc                                      <  1:2895394/100‑1 (MQ=255)
                                                                cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                     >  1:2788773/1‑100 (MQ=255)
                                                                cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                     <  1:2468798/100‑1 (MQ=255)
                                                                 gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTCCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCgg                                    <  2:3054741/100‑1 (MQ=255)
                                                                  aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCggn                                   <  1:2541346/100‑2 (MQ=255)
                                                                  aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                   <  1:3414312/100‑1 (MQ=255)
                                                                     aCCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                >  1:1295626/1‑100 (MQ=255)
                                                                     aCCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                >  2:687859/1‑100 (MQ=255)
                                                                     aCCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                >  2:3332285/1‑100 (MQ=255)
                                                                       cTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATcc                              <  2:304315/100‑1 (MQ=255)
                                                                         gggTAAAGAAGTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCa                            >  2:550972/1‑100 (MQ=255)
                                                                           gTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAc                          >  1:3436013/1‑100 (MQ=255)
                                                                            tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCTCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                         <  2:3611374/100‑1 (MQ=255)
                                                                            tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                         <  2:1334450/100‑1 (MQ=255)
                                                                            tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                         <  1:3541635/100‑1 (MQ=255)
                                                                              aagaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg                       <  2:1598427/100‑1 (MQ=255)
                                                                                  agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                   <  1:720065/100‑1 (MQ=255)
                                                                                  agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                   <  1:3188386/100‑1 (MQ=255)
                                                                                  agTTCTCGAATCCCGTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                   <  1:2229205/100‑1 (MQ=255)
                                                                                   gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                  >  1:1518214/1‑100 (MQ=255)
                                                                                      ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa               <  2:841397/100‑1 (MQ=255)
                                                                                       tcGAATCCTGTCTGCCGTGTATCCTGGAGCTTTCCCGTACCTTCGCTCACTTCGATCCGGTGCAAGAGCCGATTCCGGTTATCCCATCCTGTCACTACa               <  2:2525621/99‑1 (MQ=255)
                                                                                        cGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatg             >  1:136317/1‑100 (MQ=255)
                                                                                         gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga            >  1:1901147/1‑100 (MQ=255)
                                                                                           aTCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgctg          >  2:972127/1‑100 (MQ=255)
                                                                                           aTCCCGTCTGCCGGGTATCCTGGAGCATTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgatg          >  1:455879/1‑100 (MQ=255)
                                                                                            tCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga            <  2:3736278/97‑1 (MQ=255)
                                                                                              ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGc       <  2:2788757/100‑1 (MQ=255)
                                                                                               cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg      <  1:1672275/100‑1 (MQ=255)
                                                                                               cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg      >  1:3279880/1‑100 (MQ=255)
                                                                                               cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg      >  1:1016271/1‑100 (MQ=255)
                                                                                               cGTCGGCCGGGTATCCTGGCGCTTGCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGcn      <  2:2205647/100‑2 (MQ=255)
                                                                                                gTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg     <  2:1887965/100‑1 (MQ=255)
                                                                                                gTCAGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg     >  1:3807967/1‑100 (MQ=255)
                                                                                                 tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGt    <  1:3609343/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAT  >  NC_000913/756787‑756985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: