Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 894,155 T→G 100% D124E (GAT→GAG potF → putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913894,1550TG99.4% 508.6 / ‑5.3 143D124E (GAT→GAGpotFputrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (70/72);  total (71/72)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 143 total aligned reads displayed.

AGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894058‑894251
                                                                                                 |                                                                                                
aGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGcc                                                                                                <  2:1573882/100‑1 (MQ=255)
aGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGcc                                                                                                <  2:1262006/100‑1 (MQ=255)
  ttGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCg                                                                                              <  2:3079526/100‑1 (MQ=255)
    gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            <  1:1681533/100‑1 (MQ=255)
    gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGAc                                                                                            <  1:3259556/100‑1 (MQ=255)
     aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACa                                                                                           <  2:1932712/100‑1 (MQ=255)
     aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACa                                                                                           <  1:2016803/100‑1 (MQ=255)
     aCTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACa                                                                                           >  1:38622/1‑100 (MQ=255)
      cTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACaa                                                                                          >  2:3366663/1‑100 (MQ=255)
       tGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAAt                                                                                         <  1:986095/100‑1 (MQ=255)
        gCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATa                                                                                        >  1:2987449/1‑100 (MQ=255)
           ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt                                                                                     <  2:3604132/100‑1 (MQ=255)
           ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAt                                                                                     >  2:1747800/1‑100 (MQ=255)
            gAGTTTTCCAGCCGTTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAtt                                                                                    <  2:4048234/100‑1 (MQ=255)
            gAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAtt                                                                                    <  1:3103390/100‑1 (MQ=255)
             aGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAAttt                                                                                   >  1:1147183/1‑100 (MQ=255)
              gTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTg                                                                                  <  2:550083/100‑1 (MQ=255)
               ttttCCAGCCGCTGGACAAAAGCAAAGTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGc                                                                                 <  2:3391315/100‑1 (MQ=255)
                tttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCt                                                                                >  1:597580/1‑100 (MQ=255)
                 ttCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTa                                                                               <  2:1546804/100‑1 (MQ=255)
                   ccAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATg                                                                             >  1:3704916/1‑100 (MQ=255)
                   ccAACCGCTGGACAAAAGCAAATTGCCGGTGTAGAAGAATCCAGACCCGGAACTGCTGAAGCTGCTCGCCAAACACGAGCCCGACAATAAATTTGc                                                                                 >  2:2899739/1‑96 (MQ=255)
                    cAGCCGCTGGACAACAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGc                                                                            <  2:2838153/100‑1 (MQ=255)
                    cAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGc                                                                            >  1:2670550/1‑100 (MQ=255)
                     aGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGcc                                                                           <  2:4110770/100‑1 (MQ=255)
                     aGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGcc                                                                           <  1:3664265/100‑1 (MQ=255)
                      gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGccc                                                                          >  1:1779855/1‑100 (MQ=255)
                       ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCt                                                                         <  2:3687537/100‑1 (MQ=255)
                       ccGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGAACCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCt                                                                         <  2:1004195/100‑1 (MQ=255)
                        cGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCta                                                                        <  2:3456192/100‑1 (MQ=255)
                          cTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCtata                                                                      <  1:818820/100‑1 (MQ=255)
                            ggACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtg                                                                    >  2:2258126/1‑100 (MQ=255)
                              aCAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATAtgtg                                                                  <  2:1759280/100‑1 (MQ=255)
                                 aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               >  2:4160805/1‑100 (MQ=255)
                                 aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               <  2:715331/100‑1 (MQ=255)
                                 aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGc                                                               <  1:3913340/100‑1 (MQ=255)
                                  aaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcg                                                              <  2:3563519/100‑1 (MQ=255)
                                   aGCAAGGTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGCGCCCGACAATAAATTTGCTATGCCTTATATGTGGGcga                                                             <  2:2572871/100‑1 (MQ=255)
                                    gCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgac                                                            <  1:2942663/100‑1 (MQ=255)
                                     cAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGcgacg                                                           <  1:452673/100‑1 (MQ=255)
                                          tGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCgg                                                      <  1:1893929/100‑1 (MQ=255)
                                          tGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCgg                                                      <  2:2468961/100‑1 (MQ=255)
                                           gCCGGAGTGGAAGAATCTCGGCCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGACCTATATGTGGGGGACGACgggg                                                     >  2:192877/1‑100 (MQ=255)
                                           gCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCggg                                                     >  1:1112356/1‑100 (MQ=255)
                                             cGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGAt                                                   <  1:1825356/100‑1 (MQ=255)
                                                aGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTgg                                                <  2:234580/100‑1 (MQ=255)
                                                aGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTgg                                                >  1:3566259/1‑100 (MQ=255)
                                                 gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               <  1:3888895/100‑1 (MQ=255)
                                                 gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               <  1:2773806/100‑1 (MQ=255)
                                                 gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGc                                               <  2:268153/100‑1 (MQ=255)
                                                  tGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                              <  1:2888842/100‑1 (MQ=255)
                                                   ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             >  2:756292/1‑100 (MQ=255)
                                                   ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCta                                             >  1:3442387/1‑100 (MQ=255)
                                                   ggAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCt                                              <  2:2594410/99‑1 (MQ=255)
                                                        aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        >  1:3806870/1‑100 (MQ=255)
                                                        aaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACg                                        <  2:3224398/100‑1 (MQ=255)
                                                        aaTCGCGACCCGGAACTGCTGAAGCTGGTCGCCAAACCCGAGCCCGACAATAAATGTGCTATGCCCTATATGTGGGCGACGACCGGGATNGGCTATAACg                                        <  1:1347268/100‑1 (MQ=255)
                                                          tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGtt                                      <  2:3953125/100‑1 (MQ=255)
                                                          tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGtt                                      <  1:2330779/100‑1 (MQ=255)
                                                           ctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTg                                     >  2:2606084/1‑100 (MQ=255)
                                                           ctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTg                                     <  1:3655814/100‑1 (MQ=255)
                                                            ncGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGa                                    >  2:2094222/2‑100 (MQ=255)
                                                             cGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAAAGTTGa                                    >  2:2053347/1‑99 (MQ=255)
                                                                cccGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATaaa                                >  2:2193687/1‑100 (MQ=255)
                                                                 ccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                               <  1:1388565/100‑1 (MQ=255)
                                                                 ccGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAg                               <  2:1791810/100‑1 (MQ=255)
                                                                  cGGAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGt                              >  2:2380412/1‑100 (MQ=255)
                                                                   ggAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGtt                             <  2:3685620/100‑1 (MQ=255)
                                                                    gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTa                            <  2:3071591/100‑1 (MQ=255)
                                                                    gAACTGCTGAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTa                            <  2:545436/100‑1 (MQ=255)
                                                                         gctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                       >  1:2615886/1‑100 (MQ=255)
                                                                          ctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCgg                      >  1:1281042/1‑100 (MQ=255)
                                                                          ctgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCgg                      >  1:2658803/1‑100 (MQ=255)
                                                                           tgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGt                     >  1:377816/1‑100 (MQ=255)
                                                                           tgAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGt                     >  1:4018829/1‑100 (MQ=255)
                                                                            gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGCCGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                    >  1:2023933/1‑100 (MQ=255)
                                                                            gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                    <  2:3224806/100‑1 (MQ=255)
                                                                            gAAGCTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTg                    <  1:2751803/100‑1 (MQ=255)
                                                                                cTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTgg                >  2:2134528/1‑100 (MQ=255)
                                                                                cTGGTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTgg                >  1:3911051/1‑100 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGGGACGACAGGGATTGGCTATAACGTTGATAAAGTGGAAGCGATggt                  >  2:3454979/1‑94 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              >  2:3548379/1‑100 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              >  1:2229973/1‑100 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              <  1:3182096/100‑1 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGc              <  1:3332268/100‑1 (MQ=255)
                                                                                  ggTCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCGATACGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAGGGTAAAGCGGTGCTGGGc              >  2:761692/1‑100 (MQ=255)
                                                                                    tCGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa            >  2:2002789/1‑100 (MQ=255)
                                                                                     cGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaa           >  1:2698970/1‑100 (MQ=255)
                                                                                     cGCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaa           >  1:1719808/1‑100 (MQ=255)
                                                                                      gCCAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaa          >  2:1164491/1‑100 (MQ=255)
                                                                                       ccAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGaaaa         >  2:4018688/1‑100 (MQ=255)
                                                                                        cAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAc        >  1:2161480/1‑100 (MQ=255)
                                                                                        cAAACACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGCGCGACGATCGGGATTGGCTATACCGTTCAAAGCGTTAAATCGGTGCTTGGGaaaa         >  2:793254/1‑98 (MQ=255)
                                                                                          aaCACGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcgc      >  1:2440888/1‑100 (MQ=255)
                                                                                            cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    <  1:2134539/100‑1 (MQ=255)
                                                                                            cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    <  1:2231371/100‑1 (MQ=255)
                                                                                            cacGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACgcgc    <  1:2779955/100‑1 (MQ=255)
                                                                                             acGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGcc   <  1:763432/100‑1 (MQ=255)
                                                                                             acGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGcc   >  1:448458/1‑100 (MQ=255)
                                                                                             acGAGCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGcc   <  1:3185187/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCC  >  NC_000913/894058‑894251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: