Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,023,771 A→T 79.2% V38E (GTA→GAA)  yccS ← putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,023,7710AT79.2% 284.1 / 67.3 133V38E (GTA→GAA) yccSputative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (15/12);  new base T (58/45);  total (73/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 135 total aligned reads displayed.

GCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGCA  >  NC_000913/1023673‑1023870
                                                                                                  |                                                                                                   
gCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTAc                                                                                                    >  2:1007007/1‑100 (MQ=255)
  gTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGGGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCan                                                                                                  <  2:3184687/100‑2 (MQ=255)
   tAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATc                                                                                                 >  1:2545164/1‑100 (MQ=255)
     atgatgAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGAGCGGTCAGCGCCGCTGCCGCAATCCCCAGCGTAAGCGGCATCGTCAGTTTTTCa                                                                                                   >  2:1709915/1‑96 (MQ=255)
      tgatgaGGTTACGCAAACGTCCCGGCCGTCGGTCATCGAGATCGGTCAGAGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGGCAGTTTTACATCAcc                                                                                              >  2:975387/1‑100 (MQ=255)
      tgatgaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCAcc                                                                                              >  1:1841581/1‑100 (MQ=255)
       gatgaGGTTTCGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCAccc                                                                                             >  2:2114372/1‑100 (MQ=255)
       gatgaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCAccc                                                                                             >  1:3291906/1‑100 (MQ=255)
         tgaGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAg                                                                                           >  1:3231164/1‑100 (MQ=255)
            ggTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGcca                                                                                        <  1:1595734/100‑1 (MQ=255)
            ggTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGcca                                                                                        >  2:386756/1‑100 (MQ=255)
             gTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGccac                                                                                       >  1:1557587/1‑100 (MQ=255)
             gTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGccac                                                                                       >  1:4120339/1‑100 (MQ=255)
              ttACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGccacc                                                                                      <  1:2205996/100‑1 (MQ=255)
                 cGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACg                                                                                   >  1:1487609/1‑100 (MQ=255)
                   cAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGa                                                                                 >  1:3257013/1‑100 (MQ=255)
                    aaaCGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGaa                                                                                <  2:1875663/100‑1 (MQ=255)
                       cGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACg                                                                             >  1:1592500/1‑100 (MQ=255)
                        gTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGc                                                                            <  1:4166463/100‑1 (MQ=255)
                        gTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGc                                                                            <  2:2216651/100‑1 (MQ=255)
                          cccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCtg                                                                          <  1:1125187/100‑1 (MQ=255)
                          cccGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCtg                                                                          >  1:318602/1‑100 (MQ=255)
                             gCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTgg                                                                       <  2:836317/100‑1 (MQ=255)
                             gCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTgg                                                                       <  2:701127/100‑1 (MQ=255)
                              ccAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGt                                                                      <  1:64077/100‑1 (MQ=255)
                               cAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGtt                                                                     >  1:933691/1‑100 (MQ=255)
                               cAGTCCGTCAGCGATATCGGTCAGCGCCGCTGCCACCATCCCCACCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGtt                                                                     <  2:1583925/100‑1 (MQ=255)
                                aGTCGGTCATCGAGATCGGTCAGCGCCGCAGCCACCATCCCCAGCGCTAGCGGAATCGCCAGTTTTACACATCCCAGCCACCACGGAAACGCTGCGGTTc                                                                    >  2:502478/1‑100 (MQ=255)
                                 gTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTcc                                                                   >  1:2684357/1‑100 (MQ=255)
                                  tCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCca                                                                  <  1:3490468/100‑1 (MQ=255)
                                   cGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcac                                                                 <  2:471279/100‑1 (MQ=255)
                                   cGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcac                                                                 >  1:3336288/1‑100 (MQ=255)
                                    ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                                <  2:3490514/100‑1 (MQ=255)
                                    ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                                >  1:4162459/1‑100 (MQ=255)
                                    ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                                <  2:3231143/100‑1 (MQ=255)
                                    ggTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCcaca                                                                <  1:3640453/100‑1 (MQ=255)
                                       cATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAg                                                             >  2:1622874/1‑100 (MQ=255)
                                        aTCGAGATCGGTCAGCGCCACTGCCACCATCCACAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgc                                                            >  1:3958178/1‑100 (MQ=255)
                                         tCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgcg                                                           >  1:3931705/1‑100 (MQ=255)
                                         tCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgcg                                                           >  1:4135682/1‑100 (MQ=255)
                                           gagaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAgcgc                                                          <  2:2099106/99‑1 (MQ=255)
                                           gagaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCa                                                         >  1:1792531/1‑100 (MQ=255)
                                           gagaTCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCa                                                         >  2:1257637/1‑100 (MQ=255)
                                               tCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaa                                                     <  1:3442698/100‑1 (MQ=255)
                                                 ggTCAGCGCCGCTGCCACCATCCCCAGTGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                   >  2:3319645/1‑100 (MQ=255)
                                                 ggTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                   <  2:1402610/100‑1 (MQ=255)
                                                 ggTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                   >  1:3438607/1‑100 (MQ=255)
                                                 ggTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaa                                                   >  1:895259/1‑100 (MQ=255)
                                                  gTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaaa                                                  <  1:2941114/100‑1 (MQ=255)
                                                  gTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaaa                                                  <  1:2940683/100‑1 (MQ=255)
                                                  gTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATaaaaa                                                  >  1:2328395/1‑100 (MQ=255)
                                                   tCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAAt                                                 <  1:3276193/100‑1 (MQ=255)
                                                   tCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAAt                                                 <  1:168210/100‑1 (MQ=255)
                                                    cAGCGCCGCTGCCACCATCCCAAGCGTTAGCGGAATCGTCAGATTTTCATCAACCAGCCACCACGGAAACGCTCTGGTTCCACAAAGCGCAATAACAATa                                                >  2:979785/1‑100 (MQ=255)
                                                      gcgcCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcg                                              >  1:3792392/1‑100 (MQ=255)
                                                        gccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcg                                            >  2:820265/1‑100 (MQ=255)
                                                        gccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcg                                            >  2:2624101/1‑100 (MQ=255)
                                                         ccgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcgc                                           <  1:3333719/100‑1 (MQ=255)
                                                          cgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcgcg                                          <  1:1257644/100‑1 (MQ=255)
                                                          cgcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATAcgcgcg                                          <  2:1654856/100‑1 (MQ=255)
                                                           gcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGt                                         >  2:3525391/1‑100 (MQ=255)
                                                           gcTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGt                                         >  2:2903606/1‑100 (MQ=255)
                                                             tgacaccaTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTaa                                       <  2:3622469/97‑1 (MQ=255)
                                                             tGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTaa                                       <  1:1629008/100‑1 (MQ=255)
                                                               ccaccaTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATa                                     <  2:187526/100‑1 (MQ=255)
                                                                caccaTCCCCAGCGTTAGCGGAATCGTCAGTTCTTCATCACCCAGCCACCCCGGCAACGCTGTGGTTCCACAAATCGCAATAAAAATACGCGCGTAATa                                     >  2:1686276/1‑99 (MQ=255)
                                                                 accaTCCCCAGCGTTAGCGTAATCGTCAGTTTTTCATCACCCAGCCACGACGGAAACGCTGTGGTTCCACAAAGCGCAATGCAAATACGCGCGTAAt                                      >  2:825820/1‑97 (MQ=255)
                                                                 accaTCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACa                                   >  1:1554873/1‑100 (MQ=255)
                                                                 accaTCCCCAGCGTTAGCGGAATCGTCAGCTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATCCGCGCCTAATAc                                    >  2:3528768/1‑99 (MQ=255)
                                                                  ccttCCCCAGCGTTAGCCGAATCGTCAGTTTTCCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAATGCGCAATAAAAATACGCGCGTAATACAg                                  <  2:1782668/97‑1 (MQ=255)
                                                                    aTCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGcc                                <  1:4172365/100‑1 (MQ=255)
                                                                     tCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGcct                               <  2:1410339/100‑2 (MQ=255)
                                                                     tCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCa                               >  2:2099883/1‑100 (MQ=255)
                                                                     tCCCCAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCa                               >  1:3794277/1‑100 (MQ=255)
                                                                       cccAGCGTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAgg                             >  2:472881/1‑100 (MQ=255)
                                                                         cAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGgcg                           <  1:3359262/100‑1 (MQ=255)
                                                                         cAGCGTGAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGgcg                           <  2:1905153/100‑1 (MQ=255)
                                                                             gTTAGCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGt                       >  2:2763845/1‑100 (MQ=255)
                                                                             gTTAGCGGAATCGTCAGTTTTGCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGt                       <  1:2960954/100‑1 (MQ=255)
                                                                               tAGCGGAATCGTCAGTTTTTCATCACCAAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTc                     >  2:3671772/1‑100 (MQ=255)
                                                                                 gCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                   >  1:1345347/1‑100 (MQ=255)
                                                                                 gCGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                   <  2:1835593/100‑1 (MQ=255)
                                                                                 gCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCa                   >  2:722659/1‑100 (MQ=255)
                                                                                  cGGAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAg                  >  1:2325755/1‑100 (MQ=255)
                                                                                  cGGAATCGTCAGTTTTTCATAAACCTGCCATCACCGAGACGCTGTGGTTCCAAAAAGCGCAATAAAAATACGCGCGTAATGCAGCCAGGCGCTGTTCCa                   <  2:1605242/99‑1 (MQ=255)
                                                                                   ggAATCGTCAGTTTTTGATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAgg                 <  2:2440820/100‑1 (MQ=255)
                                                                                    gAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGt                <  1:19337/100‑1 (MQ=255)
                                                                                    gAATCGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGt                <  1:635133/100‑1 (MQ=255)
                                                                                    gAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGt                >  2:2706046/1‑100 (MQ=255)
                                                                                      aTCGTCAGTTTTGCATCACCCAGCCACCACTAAAACGCCGTGGTTCCACCTAGCGCAATACCAATACGCGCCTAATACAGCCAGGCGCTGTTCCacg                 <  2:4131791/97‑3 (MQ=255)
                                                                                       tCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCCGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGtata             <  1:1810838/100‑1 (MQ=255)
                                                                                        cGTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAg            <  1:795828/100‑1 (MQ=255)
                                                                                         gTCAGTTTTTCGTCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc           >  2:2178639/1‑100 (MQ=255)
                                                                                         gTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc           >  1:3196538/1‑100 (MQ=255)
                                                                                         gTCAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGc           >  1:200870/1‑100 (MQ=255)
                                                                                           cAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGt         >  2:4071088/1‑100 (MQ=255)
                                                                                           cAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGt         >  2:4070991/1‑100 (MQ=255)
                                                                                           cAGTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGt         >  2:3065503/1‑100 (MQ=255)
                                                                                             gTTTTTCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGttt       >  2:679264/1‑100 (MQ=255)
                                                                                                  aCATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGCa  <  1:428913/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTGTGGTTCCACAAAGCGCAATAAAAATACGCGCGTAATACAGCCAGGCGCTGTTCCAGGTATAGCGTTTGAGCA  >  NC_000913/1023673‑1023870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑