Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,035,846 C→A 15.2% A26E (GCG→GAG)  hyaD → hydrogenase 1 maturation protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,035,8460CA15.2% 329.0 / 36.1 121A26E (GCG→GAG) hyaDhydrogenase 1 maturation protease
Reads supporting (aligned to +/- strand):  ref base C (49/52);  new base A (8/11);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAG  >  NC_000913/1035747‑1035945
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aaaaTAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGc                                                                                                     >  1:330323/1‑100 (MQ=255)
     aaGTGACAAGGAGCGTTCATGAGCGCGCAACGCGTGGTGGTCATGGGGCTGGGCAACCGGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCgg                                                                                                   <  2:404735/97‑1 (MQ=255)
      aGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACg                                                                                               >  1:176012/1‑100 (MQ=255)
       gTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACgg                                                                                              >  1:507304/1‑100 (MQ=255)
       gTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACgg                                                                                              >  2:81852/1‑100 (MQ=255)
         aaCAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTAATGGGGCTGGGCAACATGCTGTCGGCCGATGAAGCCTGCGGCCTGCGGTTGGCTGAACGGCt                                                                                            >  2:853975/1‑100 (MQ=255)
          aCAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCGGATGAAGGCTTCGGCGTGCGGGGGGGTGAGCGGcgg                                                                                           >  1:1230999/1‑98 (MQ=255)
          aCAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTg                                                                                           <  2:3226305/100‑1 (MQ=255)
           cAAGGATCGTTCATGAGCGAGCAACGCGAGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTg                                                                                           <  1:2946756/99‑1 (MQ=255)
           cAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTg                                                                                           >  1:1834223/1‑99 (MQ=255)
            aaGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTa                                                                                         <  1:3455139/100‑1 (MQ=255)
            aaGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTa                                                                                         <  1:3977341/100‑1 (MQ=255)
             aGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTAt                                                                                        <  1:2490692/100‑1 (MQ=255)
                 gCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGccc                                                                                    >  1:2988276/1‑100 (MQ=255)
                 gCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGccc                                                                                    >  1:915525/1‑100 (MQ=255)
                  cGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCa                                                                                   <  1:3126622/100‑1 (MQ=255)
                      cATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTa                                                                                >  1:3134285/1‑99 (MQ=255)
                       aTGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTAcc                                                                              >  1:81814/1‑100 (MQ=255)
                       aTGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTAcc                                                                              <  2:3596633/100‑1 (MQ=255)
                       aTGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTAcc                                                                              <  2:2296571/100‑1 (MQ=255)
                         gagcgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCAc                                                                            >  2:4050138/1‑100 (MQ=255)
                           gcgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTg                                                                          >  1:1451307/1‑100 (MQ=255)
                           gcgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTg                                                                          <  2:1115631/100‑1 (MQ=255)
                            cgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTgg                                                                         >  1:2320193/1‑100 (MQ=255)
                             gagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGc                                                                        >  2:1408570/1‑100 (MQ=255)
                             gagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGc                                                                        >  2:1716321/1‑100 (MQ=255)
                              agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGcn                                                                       <  1:4114543/100‑2 (MQ=255)
                              agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGcc                                                                       <  1:1468476/100‑1 (MQ=255)
                              agcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGcc                                                                       >  1:1290977/1‑100 (MQ=255)
                               gcAACGCGTGGTGGTCATGGGGCTGGGCAGCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGccc                                                                      <  2:1206431/100‑1 (MQ=255)
                               gcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGccc                                                                      >  1:2299139/1‑100 (MQ=255)
                               gcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGccc                                                                      >  1:61548/1‑100 (MQ=255)
                               gcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGccc                                                                      >  2:1257236/1‑100 (MQ=255)
                                 aaagcgTGGTGGTCATGGGGCTGGGCAACATGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCAGTACCACTGGCCCg                                                                     <  2:1802605/96‑1 (MQ=255)
                                  acacgTGGTGGTCATGGGGATGGGCAACCTGATTTGGTCCGGTGGAGGCTTCGGCTTGCGTGTAGCGGGACGGCGGTATGCCCATTACCACTGGcccta                                                                    >  2:3479565/4‑97 (MQ=255)
                                  aCGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAg                                                                   <  1:1476956/100‑1 (MQ=255)
                                   cgcgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGt                                                                  <  1:956546/100‑1 (MQ=255)
                                    gcgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTa                                                                 <  2:1160930/100‑1 (MQ=255)
                                    gcgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTa                                                                 >  1:4009326/1‑100 (MQ=255)
                                     cgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTAt                                                                >  1:736995/1‑100 (MQ=255)
                                       tggtggTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTAtgt                                                              >  1:1598154/1‑100 (MQ=255)
                                        ggtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTAtgtg                                                             <  1:475500/100‑1 (MQ=255)
                                        ggtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTAtgt                                                              >  1:4164694/1‑99 (MQ=255)
                                         gtggtCATGGGGCTGGGCAGCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTgg                                                            <  2:1465597/100‑1 (MQ=255)
                                         gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTgg                                                            <  1:250040/100‑1 (MQ=255)
                                          tggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGga                                                           <  1:2651926/100‑1 (MQ=255)
                                          tggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGga                                                           <  1:2596688/100‑1 (MQ=255)
                                           ggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGgag                                                          >  2:2122475/1‑100 (MQ=255)
                                           ggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGgag                                                          >  1:1815497/1‑100 (MQ=255)
                                              cATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGCGTATGGGGGGAt                                                        >  2:3030665/1‑99 (MQ=255)
                                              cATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGAtt                                                       >  1:1593831/1‑100 (MQ=255)
                                              cATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGAtt                                                       >  2:1405866/1‑100 (MQ=255)
                                                tGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGt                                                     >  2:2290608/1‑100 (MQ=255)
                                                 ggggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTc                                                    <  1:276705/100‑1 (MQ=255)
                                                 ggggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTc                                                    <  2:115342/100‑1 (MQ=255)
                                                  gggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCg                                                   <  2:1044360/100‑1 (MQ=255)
                                                  gggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCg                                                   >  1:982382/1‑100 (MQ=255)
                                                       gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGc                                              <  2:3500515/100‑1 (MQ=255)
                                                       gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGc                                              <  1:1060040/100‑1 (MQ=255)
                                                       gggCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGc                                              >  2:4162967/1‑100 (MQ=255)
                                                         gCAACCTGCTGTGGGCCGCAGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCGCGCGTAGGTGGAGATTGTCGATGGCgg                                            >  2:3973899/1‑100 (MQ=255)
                                                         gCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCgg                                            <  1:1682501/100‑1 (MQ=255)
                                                         gCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCgg                                            >  2:2712642/1‑100 (MQ=255)
                                                         gCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCgg                                            <  1:4061119/100‑1 (MQ=255)
                                                          cAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCAGAGTATGTGGAGATTGTCGATGGCGGt                                           >  2:1174270/1‑100 (MQ=255)
                                                           atccTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCgg                                            <  2:3876074/96‑1 (MQ=255)
                                                           aaCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGGGTATGTGGAGATTGTCGAAGGCAGTa                                          >  2:1317819/1‑100 (MQ=255)
                                                            aCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTAc                                         <  1:3317685/100‑1 (MQ=255)
                                                              ctgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTc                                       >  1:1026155/1‑100 (MQ=255)
                                                               tgctgTGGGCCGATGTAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTGCTc                                       >  1:2851048/1‑99 (MQ=255)
                                                               tgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCa                                      <  1:296101/100‑1 (MQ=255)
                                                               tgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCa                                      <  1:4056672/100‑1 (MQ=255)
                                                                  tgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCGCGAGTATGTGGAGATTGTCGATGGCGGTACTCAggg                                   >  2:3205945/1‑100 (MQ=255)
                                                                  cgtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGGGTATGTGGAGATTGTCGATGGCGGGACTCAggg                                   >  1:1620472/2‑100 (MQ=255)
                                                                   gtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGa                                  >  1:1044434/1‑100 (MQ=255)
                                                                    tgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGAc                                 <  1:2855708/100‑1 (MQ=255)
                                                                     gggCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACt                                >  2:276939/1‑100 (MQ=255)
                                                                        ccGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGaa                             <  2:4083296/100‑1 (MQ=255)
                                                                         cGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGTACTGAAc                            <  2:736991/100‑1 (MQ=255)
                                                                          gATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACt                           <  1:1408578/100‑1 (MQ=255)
                                                                             gAAGGCTTCGGCGTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTg                         <  1:4163001/99‑1 (MQ=255)
                                                                                ggCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCTCTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTgn                     <  2:2660366/100‑2 (MQ=255)
                                                                                ggCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTgg                     <  1:433450/100‑1 (MQ=255)
                                                                                  cTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTgggg                   <  1:4081253/100‑1 (MQ=255)
                                                                                  cTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTgggg                   >  1:1806800/1‑100 (MQ=255)
                                                                                    tCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTa                 <  1:3818631/100‑1 (MQ=255)
                                                                                    tCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTa                 >  2:391008/1‑100 (MQ=255)
                                                                                        cGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTc             <  2:4009296/100‑1 (MQ=255)
                                                                                         gTGCGGGTGGCGGAACGGCTGTATGCCCATTATCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg            <  1:2957116/100‑1 (MQ=255)
                                                                                         gTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg            <  1:195949/100‑1 (MQ=255)
                                                                                         gTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg            >  1:2198474/1‑100 (MQ=255)
                                                                                         gTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg            <  1:609223/100‑1 (MQ=255)
                                                                                         gTGCGGGTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCg            <  2:3942228/100‑1 (MQ=255)
                                                                                          tGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTc             >  2:574717/1‑98 (MQ=255)
                                                                                          tGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGa           >  1:2603551/1‑100 (MQ=255)
                                                                                             gggTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAg        <  1:3748968/100‑1 (MQ=255)
                                                                                             gggTGGAGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAg        <  1:2371741/100‑1 (MQ=255)
                                                                                              ggTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgc       >  1:2871464/1‑100 (MQ=255)
                                                                                               gTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAgcg      >  2:3012626/1‑100 (MQ=255)
                                                                                                   cGGAACGGCTGTATGACCATTACCACTGGACCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGCGACTGAACTTGCTGGGGTATGTCGAAAGCgccag  <  2:2000341/100‑1 (MQ=255)
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AAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAG  >  NC_000913/1035747‑1035945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: