Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,070,115 C→A 74.0% P80P (CCG→CCT)  rutF ← flavin:NADH reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,070,1150CA74.0% 197.5 / 100.4 130P80P (CCG→CCT) rutFflavin:NADH reductase
Reads supporting (aligned to +/- strand):  ref base C (14/19);  new base A (48/46);  total (63/67)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.76e-01

Only 100 of 130 total aligned reads displayed.

GCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATG  >  NC_000913/1070017‑1070212
                                                                                                  |                                                                                                 
 ccTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAgg                                                                                                 <  1:3059155/100‑1 (MQ=255)
  ctctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGc                                                                                                >  1:750634/1‑100 (MQ=255)
   tctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCt                                                                                               <  1:1045159/100‑1 (MQ=255)
   tctTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCt                                                                                               >  2:1881201/1‑100 (MQ=255)
    ctcccAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCt                                                                                               <  2:3885750/96‑1 (MQ=255)
    ctTCCAGTTGCGGGCATCCGGTCA‑CCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTcc                                                                                             <  1:1737975/100‑1 (MQ=255)
       ccAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTg                                                                                           >  1:2234607/1‑100 (MQ=255)
          gTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGGGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGc                                                                                          >  2:1438585/1‑98 (MQ=255)
           ttGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCg                                                                                       <  2:4090766/100‑1 (MQ=255)
            tGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCgg                                                                                      >  1:578394/1‑100 (MQ=255)
            tGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCgg                                                                                      >  1:1840278/1‑100 (MQ=255)
            tGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCgg                                                                                      <  1:139403/100‑1 (MQ=255)
             gCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGgc                                                                                     >  2:3690719/1‑100 (MQ=255)
                ggCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCt                                                                                  <  2:2784970/100‑1 (MQ=255)
                 gCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTa                                                                                 <  2:1098331/100‑1 (MQ=255)
                  cATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTaa                                                                                >  2:2373709/1‑100 (MQ=255)
                  cATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTaa                                                                                >  1:1787206/1‑100 (MQ=255)
                   aTCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAg                                                                               <  1:2996076/100‑1 (MQ=255)
                   aTCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAg                                                                               >  2:2328260/1‑100 (MQ=255)
                   aTCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAg                                                                               >  2:2429357/1‑100 (MQ=255)
                    tCCGATCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAgt                                                                              <  2:1161493/100‑1 (MQ=255)
                         tCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTAtt                                                                         <  2:2334455/100‑1 (MQ=255)
                          cACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTAttt                                                                        <  1:1731063/100‑1 (MQ=255)
                          cACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTAttt                                                                        >  2:1814958/1‑100 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                      <  2:1356741/100‑1 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                      >  1:2067325/1‑100 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                      >  1:3579555/1‑100 (MQ=255)
                            cccccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCAGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                      >  1:2037592/1‑100 (MQ=255)
                             ccccGTCTGCCAGCGGGCGGCGGCCAAGCGGTGTTCCATGGGCGGTTTGCCGCCACAAACGTTTGAAAGGGACAACTGCCCGGCGCTAAGTGTATTTac                                                                      >  2:3516127/1‑99 (MQ=255)
                              cccGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                    >  2:3572668/1‑100 (MQ=255)
                              cccGCCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAACAAGGTTTGACAGAGCCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                    <  2:2427986/100‑1 (MQ=255)
                                cGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTaca                                                                     >  1:1861696/1‑97 (MQ=255)
                                  tCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAg                                                                <  1:1504230/100‑1 (MQ=255)
                                    tGCCAGCGTGCGGCGGCAAAGCCGTGTTCCATGGGCGTTTTGCCGCTAAAAAGGTTTGAAAGAGGCTCCTTCCCTGCGCTAAGTGTATTTACACACAGCg                                                              <  2:372994/100‑1 (MQ=255)
                                    tGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCg                                                              <  1:3690746/100‑1 (MQ=255)
                                    tGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCC‑AAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGt                                                             >  2:1512617/1‑100 (MQ=255)
                                      ccAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGtt                                                            <  2:1679275/100‑1 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                           <  1:365007/100‑1 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                           >  2:3756386/1‑100 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCACCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                           >  2:504249/1‑100 (MQ=255)
                                       cAGCGGGCGGCGGCAAAGCGGTGTTCCATAGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTc                                                           <  2:2500149/100‑1 (MQ=255)
                                            ggcggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGAttt                                                      >  2:2963255/1‑100 (MQ=255)
                                             gcggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGAtttt                                                     <  1:320052/100‑1 (MQ=255)
                                              cggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTc                                                    >  2:3906824/1‑100 (MQ=255)
                                               ggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCa                                                   <  2:3557126/100‑1 (MQ=255)
                                               ggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCa                                                   <  1:1087831/100‑1 (MQ=255)
                                               ggcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACACCGTTCGAttttta                                                   <  1:2832292/100‑3 (MQ=255)
                                                gcggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGTCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCAt                                                  >  2:2681364/1‑100 (MQ=255)
                                                gcggcAAAGCGGTGTTCCAGGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCAt                                                  <  1:2004071/100‑1 (MQ=255)
                                                  ggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCTAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTg                                                <  2:1557079/100‑1 (MQ=255)
                                                  ggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTg                                                <  1:2880293/100‑1 (MQ=255)
                                                  ggcAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTg                                                >  1:122477/1‑100 (MQ=255)
                                                    cAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGaa                                              >  1:690368/1‑100 (MQ=255)
                                                      aaGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATg                                            <  1:3577463/100‑1 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                        >  2:3205070/1‑100 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                        <  2:1825077/100‑1 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                        >  2:628257/1‑100 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                        >  1:1941683/1‑100 (MQ=255)
                                                          ggTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCgg                                        >  1:2360307/1‑100 (MQ=255)
                                                                ccATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgac                                  >  1:3323625/1‑100 (MQ=255)
                                                                  ntGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacgg                                >  1:2348555/2‑100 (MQ=255)
                                                                    gggCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacggac                              >  2:3994349/1‑100 (MQ=255)
                                                                    gggCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacggac                              <  2:4126490/100‑1 (MQ=255)
                                                                    gggCGTTTTGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacggac                              <  1:3028308/100‑1 (MQ=255)
                                                                           ttGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCAc                       <  1:2799176/100‑1 (MQ=255)
                                                                           ttGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCAc                       <  1:871958/100‑1 (MQ=255)
                                                                            tGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACg                      >  1:487977/1‑100 (MQ=255)
                                                                            tGCCGCCAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACg                      >  2:1586123/1‑100 (MQ=255)
                                                                              ccgccAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGAt                    >  2:3984404/1‑100 (MQ=255)
                                                                               cgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGAtt                   <  1:1093521/100‑1 (MQ=255)
                                                                               cgccAAAAAGGTTTGAAAGAGGCTCCTGCCCGGAGCTAAGTGTATTTACACACAGGGTTCGATTTTCATTGAATGCAGGCCAGACCGACGCCCCACGAtt                   >  2:2018082/1‑100 (MQ=255)
                                                                                gccAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTc                  >  1:1371051/1‑100 (MQ=255)
                                                                                  cAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAg                <  1:575344/100‑1 (MQ=255)
                                                                                  cAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAg                >  2:205302/1‑100 (MQ=255)
                                                                                  cAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAg                >  2:1220248/1‑100 (MQ=255)
                                                                                  cAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAg                >  2:1251176/1‑100 (MQ=255)
                                                                                  cAAAAAGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAg                <  1:223003/100‑1 (MQ=255)
                                                                                   aaaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAGGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAgg               <  2:3766172/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGTCCAGACGGACGCCCCACGATTCAGGc              <  1:2544064/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc              <  2:302168/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc              <  1:2391911/100‑1 (MQ=255)
                                                                                    aaaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc              <  2:2076899/100‑1 (MQ=255)
                                                                                     aaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGca             >  1:3793442/1‑100 (MQ=255)
                                                                                     aaaGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGca             <  1:493035/100‑1 (MQ=255)
                                                                                     aaaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGca             >  1:3579546/1‑100 (MQ=255)
                                                                                      agggattGAAATGGGCTCCTCCCCGGCGCGGAGAGTATTTACACACAGCGTTCGATTTTCATTGTGTGCCTGCCAGACGGACGCCCCACGATTCAGGcac            <  2:777638/95‑1 (MQ=255)
                                                                                      aatgTTTGAAAGGGACTCCTGCCCGGCGCTAAGTGTATTTACACACAGAGTTCGATCTTCATTGACTGCCGGCCAGACGGACGCCCCACGATTCAGGcac            <  2:779234/97‑1 (MQ=255)
                                                                                      aaGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGcac            <  1:3862658/100‑1 (MQ=255)
                                                                                       aGGTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGcaca           <  1:1375399/100‑1 (MQ=255)
                                                                                        ggTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCacac          <  2:2456594/100‑1 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc         <  1:2550794/100‑1 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc         >  2:4085795/1‑100 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc         <  2:2130017/100‑1 (MQ=255)
                                                                                         gTTTGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACAcc         >  2:4133417/1‑100 (MQ=255)
                                                                                           ttGAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAg       >  1:327974/1‑100 (MQ=255)
                                                                                             gAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTa     <  1:3754919/100‑1 (MQ=255)
                                                                                             gAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTa     >  1:2731362/1‑100 (MQ=255)
                                                                                             gAAAGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTa     >  1:3609985/1‑100 (MQ=255)
                                                                                              aaaGAGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTaa    >  2:3943605/1‑100 (MQ=255)
                                                                                                aGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAAtg  <  2:1271881/100‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATG  >  NC_000913/1070017‑1070212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑