BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,079,672	G→A	16.3%	R123H (CGC→CAC)	putP →	proline:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,079,672	0	G	A	16.3%	311.1 / 42.8	137	R123H (CGC→CAC)	putP	proline:sodium symporter
Reads supporting (aligned to +/- strand): ref base G (53/60); new base A (11/11); total (65/72)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 142 total aligned reads displayed.

GGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTACC  >  NC_000913/1079573‑1079769

ggAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCg                                                                                                   <  2:16790/100‑1 (MQ=255)

ggAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCg                                                                                                   >  1:3642428/1‑100 (MQ=255)

ggAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCg                                                                                                   <  2:3214985/100‑1 (MQ=255)

ggAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCg                                                                                                   >  1:691734/1‑100 (MQ=255)

 gAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGc                                                                                                  <  2:2323169/100‑1 (MQ=255)

     cTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACAttt                                                                                              >  2:2168707/1‑100 (MQ=255)

      tggtggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCAtttt                                                                                             >  1:7569/1‑100 (MQ=255)

        gtggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTgc                                                                                           >  1:3398300/1‑100 (MQ=255)

         tggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTgcg                                                                                          <  1:4163596/100‑1 (MQ=255)

          ggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTgcgn                                                                                         <  1:1080408/100‑2 (MQ=255)

          ggCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTgcgc                                                                                         >  2:2895640/1‑100 (MQ=255)

           gCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCa                                                                                        <  1:2407553/100‑1 (MQ=255)

             cGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCAtt                                                                                      >  2:523100/1‑100 (MQ=255)

               ggCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTAt                                                                                    >  1:868000/1‑100 (MQ=255)

                gCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTAtc                                                                                   >  1:4075240/1‑100 (MQ=255)

                gCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTAtc                                                                                   <  1:801737/100‑1 (MQ=255)

                   gTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTAtctc                                                                                 <  1:1967034/99‑1 (MQ=255)

                   gTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATctct                                                                                >  2:2339433/1‑100 (MQ=255)

                      gCGTGTGCATACCGAATACCACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTgcg                                                                             <  2:1918408/100‑1 (MQ=255)

                       cGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTgcgc                                                                            >  1:3777937/1‑100 (MQ=255)

                        gtgtGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTg                                                                               <  2:2198325/96‑1 (MQ=255)

                        gtgtGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCt                                                                           <  1:3323991/100‑1 (MQ=255)

                         tgtgCATACCGAATACAACAATAACGCCTTAACACTGCCGGCTTATTTCACCGGGCGCTTTGAAGCTAAAAGCCGCATTTTGCGCATTATCTCTgc                                                                              >  2:1089404/1‑96 (MQ=255)

                          gtgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTgg                                                                         >  1:2847395/1‑100 (MQ=255)

                          gtgCATACCGAATACAACAATAACGCCTTAACACGGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCCCATTTTGCGCATTATCTCTGCGCTgg                                                                         >  2:1921351/1‑100 (MQ=255)

                           tgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGt                                                                        <  1:830128/100‑1 (MQ=255)

                           tgCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGt                                                                        >  2:2135016/1‑100 (MQ=255)

                            gCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGtt                                                                       <  2:7568/100‑1 (MQ=255)

                               tACCTAATACAACATTAACGCTTTAATACTGCCGCGTTATTTCACCGTGCTCTTTTAATATATAAGCGGCATTTTTCGCATTTTCTCTGCGCTGGTTAtt                                                                    >  2:1649682/1‑100 (MQ=255)

                               tACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTAtt                                                                    >  1:3818122/1‑100 (MQ=255)

                                aCCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTAttt                                                                   <  2:1815103/100‑1 (MQ=255)

                                aCCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTAttt                                                                   >  1:1075023/1‑100 (MQ=255)

                                 ccGAATACAACAATAGCGCCTTAACGCTGCGGGATTATTTCAGCGGGTGCTTTGTAGATAAAAGCCGCATTTTGCGCATAATCTCTGCGCTGGTTAttt                                                                   <  2:1815054/99‑1 (MQ=255)

                                 cagAATACAACAATAACGCCTTAACACTACCGTATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTAtttt                                                                  <  1:2067522/98‑1 (MQ=255)

                                   gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                                >  1:3870956/1‑100 (MQ=255)

                                   gAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGc                                                                <  1:3071415/100‑1 (MQ=255)

                                     aTACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTg                                                              <  2:711214/100‑1 (MQ=255)

                                     aTACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTg                                                              <  2:381590/100‑1 (MQ=255)

                                        caacaaTAACGCCTTAACACTGCCGGCTTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCCTTATCTCTGCGCTGGTTATTTTGCTGttc                                                           >  2:3696891/1‑100 (MQ=255)

                                        caacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttc                                                           >  2:2707721/1‑100 (MQ=255)

                                        caacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttc                                                           <  2:1484529/100‑1 (MQ=255)

                                        caacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttc                                                           >  2:3202035/1‑100 (MQ=255)

                                        caacaaTAACGCCTTAACAAGGCCGGATGATTTCACCGGTCGCAGTGAAGATAACAGCCGCATTTTGCGCATGATCTCGGCGCTGGCTAtttggttgctc                                                           >  2:2408442/1‑92 (MQ=255)

                                         aacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                          <  1:2415754/100‑1 (MQ=255)

                                         aacaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttct                                                          <  1:518077/100‑1 (MQ=255)

                                          acaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttctt                                                         >  1:1599430/1‑100 (MQ=255)

                                          acaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttctt                                                         >  1:923419/1‑100 (MQ=255)

                                          acaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATTTCTGCGCTGGTTATTTTGCTGttctt                                                         >  1:957094/1‑100 (MQ=255)

                                          acaaTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCAGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGttctt                                                         >  2:954051/1‑100 (MQ=255)

                                           caaTAACGCCTTAACACTGCCGGATTAATTCACCGGGCGCTTTGAAGATAAAAACCGCATTTTGCGCAATATCTCTGCGATGGTTACTTTGCTGttctt                                                         >  1:2424761/1‑99 (MQ=255)

                                            aaTAACGCCTTAACACTGCCGGATTATTTCACAGGGCGCTTTTAAGATAAAAGCCGCATTTTGCGCATTATCTGTGCGCTGGTTCTTTTGCt                                                               >  2:3433036/1‑92 (MQ=255)

                                             aTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCAc                                                      >  1:2675608/1‑100 (MQ=255)

                                             aTAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCAc                                                      <  2:1895049/100‑1 (MQ=255)

                                              tAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCAcc                                                     <  2:20586/100‑1 (MQ=255)

                                               agcGCCGTAACACTGCCGGATTATTTCACCGGGCGCTTTTAAGATAACAGCCGCATTTTGCGGATTACCTCTTCGCTGGTTATTTTGCTGTTGTTCAcc                                                     >  2:3182922/3‑99 (MQ=255)

                                                   ccTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTa                                                <  1:3578387/100‑1 (MQ=255)

                                                    cTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTAt                                               <  1:2091740/100‑1 (MQ=255)

                                                       aaCACTGCCGTATTATTGCACCGGGCGCTTTGAAGATAATAGCCCCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTg                                             <  2:1334338/99‑1 (MQ=255)

                                                        acacTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcg                                           <  1:3202171/100‑1 (MQ=255)

                                                         cacTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcgc                                          <  2:372550/100‑1 (MQ=255)

                                                         cacTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcgc                                          >  1:3278724/1‑100 (MQ=255)

                                                             gCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCg                                      >  1:3762236/1‑100 (MQ=255)

                                                              ccGGATTATTTCGCCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTgcg                                           <  2:3791733/94‑1 (MQ=255)

                                                              ccGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCgg                                     <  1:1962931/100‑1 (MQ=255)

                                                               cGGATTATTTCACCGGGCGCTTTGAAGATAATAGCCACATTTTGCGCATTATCTCTGCGTTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCggg                                    <  2:108870/100‑1 (MQ=255)

                                                                    cttttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtg                               <  1:2610469/98‑1 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTCTTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                             >  2:330498/1‑100 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                             <  1:2333422/100‑1 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                             >  2:2808460/1‑100 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                             >  1:1315546/1‑100 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgag                             >  2:2610454/1‑98 (MQ=255)

                                                                      tttCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATtgtg                             >  2:4046391/1‑100 (MQ=255)

                                                                       ttCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTgg                            <  2:1635622/100‑1 (MQ=255)

                                                                       ttCACCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTgg                            >  1:317671/1‑100 (MQ=255)

                                                                        tCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGc                           >  1:4159795/1‑100 (MQ=255)

                                                                         cACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCa                          <  2:2680671/100‑1 (MQ=255)

                                                                          aCCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAg                         >  1:3504103/1‑100 (MQ=255)

                                                                          aCCGGGCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAg                         <  2:1124872/100‑1 (MQ=255)

                                                                            cGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGTAGgc                       >  1:1161875/1‑100 (MQ=255)

                                                                              ggCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgc                     >  2:4127225/1‑100 (MQ=255)

                                                                              ggCGCTTTGAAGATAAAAGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgc                       <  2:3059965/98‑1 (MQ=255)

                                                                               gcgcTTTGAAGATAAAAGCTGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg                    >  2:2588853/1‑100 (MQ=255)

                                                                               gcgcTTTGAAGATAAAAGCTGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg                    >  2:547674/1‑100 (MQ=255)

                                                                               gcgcTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg                    <  2:923415/100‑1 (MQ=255)

                                                                               gcgcTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGgcgcg                    <  2:572102/100‑1 (MQ=255)

                                                                                   tttGAAGATAAAAGCCACATTTTGCGCATTCTTTCTCCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTc                <  2:2266176/100‑1 (MQ=255)

                                                                                    ttGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCt               >  2:1475192/1‑100 (MQ=255)

                                                                                    ttGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCt               >  1:1233105/1‑100 (MQ=255)

                                                                                        aGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGttt           <  2:3646318/100‑1 (MQ=255)

                                                                                         gagagccgCCGCATTTTGCGCTGTAGCTCGGCGCTGGTCGGCTTGTGGTGTTTCACCAGATTTTGCGTTGCGGGCATTGTGGCAGTCGCGCGTCTgn             <  2:3460887/90‑2 (MQ=255)

                                                                                         gATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTg          >  2:3698309/1‑100 (MQ=255)

                                                                                         gATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTg          <  2:1300451/100‑1 (MQ=255)

                                                                                           tAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGaa        >  2:2367104/1‑100 (MQ=255)

                                                                                           tAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTCGCTGTTCTTCACCATTTATTGCGCTTCGGGCTTTGTGGCAGGCGCCAGTCTGTTTgca        >  2:200090/1‑98 (MQ=255)

                                                                                              aaGCCACATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGt     >  1:615679/1‑100 (MQ=255)

                                                                                               aGCCGCATTTTGCGCACTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTa    <  2:2436259/100‑1 (MQ=255)

                                                                                                tccGCATTTTGTGCATTATCTCTGTTCTGGTGATTTTGCTGTGCGTCACCATTTATTGCTCTTCGAGCATTGTGGCAGGCGCGCGTCTGTTTGaa        <  2:1982140/94‑1 (MQ=255)

                                                                                                gCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTAc   >  2:2831913/1‑100 (MQ=255)

                                                                                                gCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTAc   <  1:2287716/100‑1 (MQ=255)

                                                                                                 ccGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTAcc  <  2:3504076/100‑1 (MQ=255)

GGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTACC  >  NC_000913/1079573‑1079769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`