Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,157 A→G 76.8% intergenic (+250/+122) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,1570AG76.8% 246.2 / 60.0 127intergenic (+250/+122)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (22/8);  new base G (42/55);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 6.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 130 total aligned reads displayed.

AGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGA  >  NC_000913/1224060‑1224253
                                                                                                 |                                                                                                
aGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGgaa                                                                                                <  1:2848343/100‑1 (MQ=255)
 gTATAGCCATACTAACATTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGgaa                                                                                                <  2:1532981/99‑1 (MQ=255)
  tataTCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATa                                                                                              <  1:309273/100‑1 (MQ=255)
   atatCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAg                                                                                             <  2:3718574/100‑1 (MQ=255)
    tatCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGc                                                                                            <  1:2225837/100‑1 (MQ=255)
    tatCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGc                                                                                            <  2:1129559/100‑1 (MQ=255)
     atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                           <  1:1802112/100‑1 (MQ=255)
     atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                           >  1:1302278/1‑100 (MQ=255)
     atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                           >  2:3738989/1‑100 (MQ=255)
     atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                           >  2:936515/1‑100 (MQ=255)
     atCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCa                                                                                           <  2:2944694/100‑1 (MQ=255)
      tCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCaa                                                                                          <  2:1620117/100‑1 (MQ=255)
        cATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATg                                                                                        <  2:4125693/100‑1 (MQ=255)
         aTACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATga                                                                                       >  2:3787043/1‑100 (MQ=255)
          tACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgag                                                                                      <  1:69260/100‑1 (MQ=255)
           aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagg                                                                                     <  1:3118738/100‑1 (MQ=255)
           aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagg                                                                                     >  2:4116284/1‑100 (MQ=255)
           aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagg                                                                                     <  2:3968879/100‑1 (MQ=255)
            cTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATgagga                                                                                    >  1:3336226/1‑100 (MQ=255)
              aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGTGGAGGTGGAAATAGCAATGAGGAGt                                                                                  >  2:1548799/1‑100 (MQ=255)
              aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGAGGTGGGAATAGCAATGAGGAGTa                                                                                 >  2:632724/1‑100 (MQ=255)
              aaCAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTATTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGt                                                                                  >  2:1848434/1‑100 (MQ=255)
                cAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgaggagca                                                                                 >  2:3423391/1‑97 (MQ=255)
                 aaTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATc                                                                               <  2:1396045/100‑1 (MQ=255)
                  aTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCa                                                                              >  2:2626079/1‑100 (MQ=255)
                   tAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAg                                                                             <  1:1535317/100‑1 (MQ=255)
                    aaGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGc                                                                            <  2:3458297/100‑1 (MQ=255)
                     aGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCa                                                                           <  1:2658947/100‑1 (MQ=255)
                     aGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCa                                                                           >  2:3525256/1‑100 (MQ=255)
                     aGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCa                                                                           >  1:1666108/1‑100 (MQ=255)
                      ggTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCaa                                                                          >  1:192220/1‑100 (MQ=255)
                       gTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                                                         <  1:1175702/100‑1 (MQ=255)
                          aTTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAAt                                                                      >  1:247501/1‑100 (MQ=255)
                              aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGCATCAGCAAGAATACTc                                                                  >  1:4126420/1‑100 (MQ=255)
                              aCTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                  >  2:3127629/1‑100 (MQ=255)
                              aCTCAACCAGGCATAAACATCTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGTGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                                                  >  2:2550259/1‑100 (MQ=255)
                                tCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgc                                                                <  2:1635076/100‑1 (MQ=255)
                                 cAACCAGGCATAAACATTTTGTTTTGTGTGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                                                               >  1:1057466/1‑100 (MQ=255)
                                 cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                                                               >  1:1658167/1‑100 (MQ=255)
                                 cAACCAGGCATAAACATATTGTAGTGTGCGTGGGAACAGCCTTAAGGTGTAGAGGGGGTGGGGGGAGTAGCACTGAGGAATGTCAGCAGGAATAGGcgcc                                                               >  2:2029356/1‑100 (MQ=255)
                                  aaCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgccg                                                              >  1:446511/1‑100 (MQ=255)
                                  aaCCAGGCATAAACATTTTGTTTGGTGTGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgc                                                                <  2:949817/98‑1 (MQ=255)
                                   aCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTcgccgc                                                             <  1:3044965/100‑1 (MQ=255)
                                     cAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCt                                                            <  2:1855646/99‑1 (MQ=255)
                                      aGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCttt                                                          <  1:991730/100‑1 (MQ=255)
                                        gCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGAGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTa                                                         >  2:356238/1‑99 (MQ=255)
                                         cATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                                                       <  2:575600/100‑1 (MQ=255)
                                          aTAAACATTTTGTATTGTGCGTGGAAACAGCCTTAAGGAGTAAAGGGTGAGGTGGGAAAAGCAATGAGGAGTATCAGCAAGAATAATCGCGGGTTTACca                                                      >  2:3450114/1‑100 (MQ=255)
                                           tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcac                                                     >  1:1435119/1‑100 (MQ=255)
                                           tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcac                                                     >  1:2908156/1‑100 (MQ=255)
                                             aaCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACaa                                                   >  1:753699/1‑100 (MQ=255)
                                               cATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACg                                                 <  1:3762544/100‑1 (MQ=255)
                                               cATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACg                                                 >  1:2588904/1‑100 (MQ=255)
                                                aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGt                                                >  2:1956197/1‑100 (MQ=255)
                                                   ttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGa                                             >  1:1476878/1‑100 (MQ=255)
                                                     gttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATg                                           >  2:563420/1‑100 (MQ=255)
                                                      ttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATga                                          >  1:1091715/1‑100 (MQ=255)
                                                        ttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgaga                                        >  1:3924820/1‑100 (MQ=255)
                                                         tgtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGagag                                       <  2:1028241/100‑1 (MQ=255)
                                                         tgtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGCGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGagag                                       >  2:2893813/1‑100 (MQ=255)
                                                          gtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAgg                                      <  1:2606067/100‑1 (MQ=255)
                                                          gtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAgg                                      >  1:343365/1‑100 (MQ=255)
                                                            gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGAGGATGAGAGGGa                                    >  2:3144839/1‑100 (MQ=255)
                                                               tGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGACGCTTTAACACAACGTGAATGAGAGGGATGa                                 >  2:3186281/1‑100 (MQ=255)
                                                                   aaCAGCATTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGaaaaa                             <  2:3606706/100‑1 (MQ=255)
                                                                     cAGCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACt                           >  1:2481875/1‑100 (MQ=255)
                                                                       gCCTTAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCa                         >  1:3712851/1‑100 (MQ=255)
                                                                           tAAGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGc                     <  1:2626094/100‑1 (MQ=255)
                                                                            aaGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGc                     <  2:962656/99‑1 (MQ=255)
                                                                            aaGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCa                    >  2:385714/1‑100 (MQ=255)
                                                                             aGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCag                   <  2:2139548/100‑1 (MQ=255)
                                                                             aGGTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCag                   <  2:1714498/100‑1 (MQ=255)
                                                                              ggTGTAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCaga                  <  2:1381977/100‑1 (MQ=255)
                                                                              ggTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCaga                  <  2:3608788/100‑1 (MQ=255)
                                                                               ttccgatctgggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCagag                 <  2:2248650/91‑1 (MQ=255)
                                                                               gtgtAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCagag                 <  1:170552/100‑1 (MQ=255)
                                                                                tgtAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                <  2:1174957/100‑1 (MQ=255)
                                                                                  tAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                <  2:2892378/98‑1 (MQ=255)
                                                                                  tAAAGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa              <  2:2090839/100‑1 (MQ=255)
                                                                                  tAAAGGGGGAGGAGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTCCCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa              >  1:1295486/1‑100 (MQ=255)
                                                                                   aaaGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa             >  1:672566/1‑100 (MQ=255)
                                                                                    aaGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc            >  1:1549604/1‑100 (MQ=255)
                                                                                    aaGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc            >  1:2399887/1‑100 (MQ=255)
                                                                                    aaGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc            <  1:1531423/100‑1 (MQ=255)
                                                                                    aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc            >  2:140583/1‑100 (MQ=255)
                                                                                     aGGGGGAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCGAGGCAGAGATAAct           >  2:2844659/1‑100 (MQ=255)
                                                                                      gggggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAAAAAAAAAAAAAACTCAAGGCAGAGATAActc          <  2:392611/100‑1 (MQ=255)
                                                                                      gggggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAActc          >  2:2521264/1‑100 (MQ=255)
                                                                                        gggAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgagagatcggaa        >  1:2248664/1‑92 (MQ=255)
                                                                                          taGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc      <  1:846754/99‑1 (MQ=255)
                                                                                          gAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATATTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc      <  1:4125757/100‑1 (MQ=255)
                                                                                          gAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGcc      <  2:4114068/100‑1 (MQ=255)
                                                                                          gAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc      <  1:686628/100‑1 (MQ=255)
                                                                                          gAGGTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc      <  1:763813/100‑1 (MQ=255)
                                                                                            ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt    <  2:4161035/100‑1 (MQ=255)
                                                                                            ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt    >  2:1666204/1‑100 (MQ=255)
                                                                                             gTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg   <  1:301626/100‑1 (MQ=255)
                                                                                             gTGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg   <  1:1948747/100‑1 (MQ=255)
                                                                                             gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg   <  1:1213028/100‑1 (MQ=255)
                                                                                              tGGGAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAACCTCAAGGCAGAGTTATCTCTGCCTTGa  <  1:3144858/100‑1 (MQ=39)
                                                                                                 |                                                                                                
AGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGA  >  NC_000913/1224060‑1224253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: