Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,944 T→C 100% intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,9440TC99.1% 388.2 / ‑4.6 116intergenic (‑22/‑583)hns/tdkglobal DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (52/63);  minor base A (0/1);  total (52/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 116 total aligned reads displayed.

CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTG  >  NC_000913/1292848‑1293036
                                                                                                |                                                                                            
cGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgg                                                                                           >  1:1124335/1‑100 (MQ=255)
 gTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTggg                                                                                          <  2:1174578/100‑1 (MQ=255)
  tttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                         <  1:3468610/100‑1 (MQ=255)
   ttCAAGTGTACATTCTCTTTCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTgggg                                                                                         <  2:3180578/99‑1 (MQ=255)
   ttCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCGTCGCTCATTGTAGTAATCTCAAACTTATACTGGggt                                                                                        <  2:3633024/100‑1 (MQ=255)
    tCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtg                                                                                       <  1:2418878/100‑1 (MQ=255)
    tCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGggtg                                                                                       <  1:1761462/100‑1 (MQ=255)
          gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                 >  1:512348/1‑100 (MQ=255)
          gtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgt                                                                                 <  1:3969994/100‑1 (MQ=255)
           tACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTttgtt                                                                                >  1:2231209/1‑100 (MQ=255)
             cATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGa                                                                              >  2:327088/1‑100 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                            <  1:1077003/100‑1 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                            <  2:2828686/100‑1 (MQ=255)
               ttCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                            >  1:1731973/1‑100 (MQ=255)
                tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAgg                                                                            <  2:2830299/99‑1 (MQ=255)
                tctcTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGGGGTTTGTTGAGGt                                                                           >  2:2733020/1‑100 (MQ=255)
                 ctctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGta                                                                          >  1:1936016/1‑100 (MQ=255)
                 ctctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGta                                                                          >  1:4051045/1‑100 (MQ=255)
                  tctgtccTGCGCAGGAAGAGTACGGATGTTGATCAGAATTTTAAGTGCGTCGCTCATTGTAGTAATCTGAAACTTATACTGGGGTATTTTGTTGAgg                                                                            <  2:2441084/92‑1 (MQ=255)
                   ctTGCCTGCGCACGAAGAGTACGGATTTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaat                                                                        <  2:2879512/100‑1 (MQ=255)
                   ctTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaat                                                                        >  1:2055677/1‑100 (MQ=255)
                    ttGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGtaata                                                                       <  2:1601715/100‑1 (MQ=255)
                      nccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTaataat                                                                     >  2:3984053/2‑100 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                    <  1:1694466/100‑1 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                    <  2:303556/100‑1 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                    <  1:2715001/100‑1 (MQ=255)
                       ccTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAataata                                                                    <  2:4053500/100‑1 (MQ=255)
                        cTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTCATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATag                                                                   >  2:1736341/1‑100 (MQ=255)
                          gcgcACGAAGAGTACGGATGTTGTTCAGAATTTTACGTGCTTTGCTCTTTGAGGTAATCTCAAACTTATACAGGGGTGGATTGTTGAGGCAACGATaga                                                                  >  1:2426342/1‑99 (MQ=255)
                          gcgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATagag                                                                 <  2:1612260/100‑1 (MQ=255)
                           cgcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGc                                                                >  1:1220094/1‑100 (MQ=255)
                           cgcACGAAGAGTACGGATGTTGTTCAGAAGTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGATGGTTTGTTGAGGTAATAATagag                                                                 <  2:2938752/99‑1 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                               <  1:1969209/100‑1 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                               <  2:4143034/100‑1 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                               <  2:1936006/100‑1 (MQ=255)
                            gcACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACGTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGcc                                                               >  1:3974838/1‑100 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                              <  1:2564012/100‑1 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                              <  1:1287267/100‑1 (MQ=255)
                             cACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATAGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCt                                                              >  2:2308294/1‑100 (MQ=255)
                               cGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTa                                                            >  1:3210365/1‑100 (MQ=255)
                                 aaGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTaaa                                                          >  1:3483911/1‑100 (MQ=255)
                                 aaGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGAGGTGGTTTGTTGAGGTAATAATAGAGCCTTaaa                                                          <  2:2866321/100‑1 (MQ=255)
                                   gagTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAAtt                                                        >  2:2908727/1‑100 (MQ=255)
                                     gTACGGATGTTGTTCAGAATTTCAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCa                                                      <  2:1451978/100‑1 (MQ=255)
                                      tACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCTTTGTCGTAATCTCAAAATTATACTGAGGTGGTTTGTTGAGGTAATAATAGGGCCTTAACTTCAg                                                     <  2:1439915/100‑1 (MQ=255)
                                       aCGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGCGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGt                                                    >  2:2799064/1‑100 (MQ=255)
                                       aCGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCGCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGt                                                    >  2:2267472/1‑100 (MQ=255)
                                        cGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                   <  1:3653505/100‑1 (MQ=255)
                                         ggATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtg                                                  <  2:2538832/100‑1 (MQ=255)
                                          gATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgt                                                 <  2:1496217/100‑1 (MQ=255)
                                           aTGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGtt                                                   <  2:3115707/97‑1 (MQ=255)
                                           aTGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTtgtg                                                <  2:2454706/100‑1 (MQ=255)
                                            tgttgtTTAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGc                                               <  2:2872485/100‑1 (MQ=255)
                                             gttgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCa                                              <  1:639221/100‑1 (MQ=255)
                                             gttgttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTAAGGTAATAATAGAGCCTTAAATTCAGTtgtg                                                <  1:3625400/98‑1 (MQ=255)
                                                gttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATa                                           >  2:379819/1‑100 (MQ=255)
                                                 ttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAg                                          >  1:1261167/1‑100 (MQ=255)
                                                  tCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGc                                         >  1:1472711/1‑100 (MQ=255)
                                                  tCAGAATTATACGAACTTCGCTCATTGTAATAATCTCAAACTTATACTGGTGTCGTATGTTGTGAACATAATAGAGACTTAAATTCAGTTGTGCa                                              >  2:2902213/1‑95 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                        <  1:1905349/100‑1 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                        <  1:3426747/100‑1 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGcc                                        >  2:2079844/1‑100 (MQ=255)
                                                   cAGAATTTTAAGTGCTTCGATCATTGTAGTAATCTCAAACTTATACTGGGGTGATTTGTTGAGGTAATAATAGAGCATTAAATTCAATTGTACAATa                                           >  2:3796118/1‑97 (MQ=255)
                                                    aaaaTTTTAAGTGCTTCTCTCATTGTAGTAATCTCAAACTTATACTGGTGTTGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                       <  2:491826/98‑1 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                       >  1:4098275/1‑100 (MQ=255)
                                                    aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCa                                       >  2:1003365/1‑100 (MQ=255)
                                                     gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                      >  1:1673002/1‑100 (MQ=255)
                                                     gAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAg                                      >  1:3029064/1‑100 (MQ=255)
                                                        ttttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGaa                                   <  1:3093315/100‑1 (MQ=255)
                                                          ttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATg                                 <  2:31384/100‑1 (MQ=255)
                                                            aaGTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTa                               >  1:1705965/1‑100 (MQ=255)
                                                              gTGCTTCGGTAATTGTAGTAATCTCAAACATATACTGGGGTGGTTTGTTGAGGTAATCATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                             <  2:2619392/100‑1 (MQ=255)
                                                              gTGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                             <  1:2244236/100‑1 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATTTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAg                             <  2:1857559/99‑1 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                            <  1:3243685/100‑1 (MQ=255)
                                                               tGCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                            <  1:1096324/100‑1 (MQ=255)
                                                               tGCTTCGCTCATGGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAgg                            <  2:121021/100‑1 (MQ=255)
                                                                gCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGa                           >  1:657301/1‑100 (MQ=255)
                                                                gCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGa                           <  2:3112542/100‑1 (MQ=255)
                                                                gCTTCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGa                           >  2:1052778/1‑100 (MQ=255)
                                                                  ttCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCATTTGTGCAATAGCCAGGAATGTAAGGAAt                         <  2:2247176/100‑1 (MQ=255)
                                                                  ttCGCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGan                          <  2:3699662/99‑2 (MQ=255)
                                                                     gCTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCa                      >  2:639399/1‑100 (MQ=255)
                                                                      cTCATTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCaa                     >  1:3761529/1‑100 (MQ=255)
                                                                         aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                  <  1:3484441/100‑1 (MQ=255)
                                                                         aTTGTAGTAATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAAt                  <  1:3333911/100‑1 (MQ=255)
                                                                            gtagtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGt               >  1:4054488/1‑100 (MQ=255)
                                                                            gtagtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGt               <  1:3131234/100‑1 (MQ=255)
                                                                               gtaATCTCAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCt            >  1:2244157/1‑100 (MQ=255)
                                                                                  aTCTCAACCTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTa         <  1:1039115/100‑1 (MQ=255)
                                                                                    ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTCAGGAATTCAAAATTGTTCTTTAtt       >  2:1216940/1‑100 (MQ=255)
                                                                                    ctcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtt       >  2:550178/1‑100 (MQ=255)
                                                                                     tcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      >  2:1755317/1‑100 (MQ=255)
                                                                                     tcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      <  2:4088992/100‑1 (MQ=255)
                                                                                     tcAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      <  1:2117955/100‑1 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt     >  1:211064/1‑100 (MQ=255)
                                                                                      cAAACTTATACTGGGGTGGTTTGTTGAGGTAATAATAGAGCATTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAt        >  2:3436377/1‑97 (MQ=255)
                                                                                       aaaCTTATACTGGGGTGGTTTGTTTATGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt      <  2:272099/98‑1 (MQ=255)
                                                                                          cTTATACTGGGGTTGTTTGTTGAGTTAATAATAGAGCCTTAAATTCAGTTTTGCAATAGCATGTAATGTAAGTAATTCAAAACTGTTCTTTATTTtgtg  <  2:2364726/99‑1 (MQ=255)
                                                                                          cTTATAATGGAGTGGTTTGTTGAGGTAATAATAGAGCAATAAATACAGTTGTGCAATAGACCGGAATGTAAGGAATTCAAAATTGTTATTTATATtgt   <  2:3749741/98‑1 (MQ=255)
                                                                                                |                                                                                            
CGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTG  >  NC_000913/1292848‑1293036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: