Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,401,844 A→G 100% I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,401,8440AG98.9% 361.5 / ‑4.8 108I471T (ATC→ACC) abgBp‑aminobenzoyl‑glutamate hydrolase, B subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (52/53);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTG  >  NC_000913/1401749‑1401940
                                                                                               |                                                                                                
acgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAgg                                                                                              >  1:65430/1‑100 (MQ=255)
 cgacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGGGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAggg                                                                                             >  2:2265649/1‑100 (MQ=255)
  gacgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGc                                                                                            <  1:1274859/100‑1 (MQ=255)
   acgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCa                                                                                           >  1:3153222/1‑100 (MQ=255)
    cgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAg                                                                                          <  1:1959260/100‑1 (MQ=255)
      gTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTg                                                                                        <  2:2751363/100‑1 (MQ=255)
       tATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGa                                                                                       <  1:3842369/100‑1 (MQ=255)
       tATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGAGTCGCTGTTGTCATTTTAAAGGTGACGGTGCCACGTTTTTCGTCGTAGGGCAGTgc                                                                                       <  1:4153710/100‑2 (MQ=255)
        aTGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGAt                                                                                      <  1:80944/100‑1 (MQ=255)
           gATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACg                                                                                   >  1:3544693/1‑100 (MQ=255)
             tGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGt                                                                                 >  2:3485305/1‑100 (MQ=255)
              gACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGtt                                                                                >  2:2247815/1‑100 (MQ=255)
                cATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGc                                                                              >  2:3237751/1‑100 (MQ=255)
                  tACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCgt                                                                            <  2:2406095/100‑1 (MQ=255)
                      cATGGGCATTCCTCGGGTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGGGTCACGTTTTTCGGTGTAGGGCAGTGATACGGtt                                                                                >  2:1501269/1‑92 (MQ=255)
                       aTGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCg                                                                       <  2:4110543/100‑1 (MQ=255)
                        tGGGCATTCCTCGTTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGGTACGGTTGCGTGTCCGt                                                                      >  2:1561572/1‑100 (MQ=255)
                         gggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTTTTTTTTTTTTAGTGATACGGTTGCGTGTCCGtt                                                                     >  2:3868429/1‑100 (MQ=255)
                         gggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGtt                                                                     >  2:3127562/1‑100 (MQ=255)
                         gggCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGtt                                                                     <  1:524968/100‑1 (MQ=255)
                            cATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACt                                                                  <  2:3680451/100‑1 (MQ=255)
                               tCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGc                                                               <  2:2192415/100‑1 (MQ=255)
                                ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCt                                                              <  2:3545243/100‑1 (MQ=255)
                                 cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                             <  1:3295360/100‑1 (MQ=255)
                                 cTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTg                                                             >  1:2356200/1‑100 (MQ=255)
                                  tCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGa                                                            <  2:1704265/100‑1 (MQ=255)
                                   cGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGAt                                                           <  1:2998771/100‑1 (MQ=255)
                                      ttgttgTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGct                                                        >  2:2386404/1‑100 (MQ=255)
                                      ttgttgTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGct                                                        <  1:3720103/100‑1 (MQ=255)
                                        gttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCCGTGATACGGTGGCGTGTCCGTTACTTGCTGATGctct                                                      >  1:1132404/1‑100 (MQ=255)
                                         ttgtGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCtt                                                     <  2:2762100/100‑1 (MQ=255)
                                          tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttg                                                    >  2:2689468/1‑100 (MQ=255)
                                           gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTATGGCAGTGATACGGTTGCGTGTGCGTTACTTGCTGATGCTCttgt                                                   >  2:181191/1‑100 (MQ=255)
                                           gtgtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGAAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGctc                                                       <  2:2959615/96‑1 (MQ=255)
                                             gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                                 <  1:730877/100‑1 (MQ=255)
                                             gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                                 >  1:97252/1‑100 (MQ=255)
                                             gtTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCttgttg                                                 >  1:3141894/1‑100 (MQ=255)
                                              tttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTgg                                                <  2:2061835/100‑1 (MQ=255)
                                              tttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTgg                                                <  2:3609315/100‑1 (MQ=255)
                                              tttGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTgg                                                <  2:3381493/100‑1 (MQ=255)
                                                  cGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCAtt                                            <  1:168405/100‑1 (MQ=255)
                                                    ttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCt                                          <  1:1116406/100‑1 (MQ=255)
                                                     tgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCCTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCtt                                         >  2:3948726/1‑100 (MQ=255)
                                                       ttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       <  2:3544666/100‑1 (MQ=255)
                                                       ttgttgttATTGTAAACGTAACTGTGTCACGGGTTTCTAGGTCGGGCAGAGATACGGTTGCGTGTCCGATACATGCTCATGCTCTTGTTGGCAGGCTTGc                                       <  2:3811373/100‑1 (MQ=255)
                                                       tcgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       <  1:2144917/98‑1 (MQ=255)
                                                       gatctgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                       <  2:2320914/96‑1 (MQ=255)
                                                        tgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCa                                      <  2:3754599/100‑1 (MQ=255)
                                                        tgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCa                                      >  2:736928/1‑100 (MQ=255)
                                                         gttgttATTTTAAAGGTGACGGTGTCACGTTTTTTGTTGTTTTTCAGTGATACGGTTGCCTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCaa                                     >  2:4022297/1‑100 (MQ=255)
                                                          ttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAAt                                    <  1:3948756/100‑1 (MQ=255)
                                                           tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   <  1:1745328/100‑1 (MQ=255)
                                                           tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   >  2:3414093/1‑100 (MQ=255)
                                                           tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   >  1:2956723/1‑100 (MQ=255)
                                                            gttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCagatc                                  >  1:2320929/1‑96 (MQ=255)
                                                            gttATTTGAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATa                                   <  1:1674933/99‑1 (MQ=255)
                                                              tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                                <  2:3397049/100‑1 (MQ=255)
                                                              tATTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGcc                                <  1:2180951/100‑1 (MQ=255)
                                                               aTTTTAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGccc                               <  1:2443583/100‑1 (MQ=255)
                                                                ttttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCt                              >  1:1055759/1‑100 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCt                              >  2:414001/1‑99 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             >  2:413538/1‑100 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             <  1:1091904/100‑1 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             >  2:2023179/1‑100 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             >  1:4172475/1‑100 (MQ=255)
                                                                 tttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTg                             >  2:1899144/1‑100 (MQ=255)
                                                                  ttAAAGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGa                            >  1:99642/1‑100 (MQ=255)
                                                                    aaaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAAt                          <  1:1798630/100‑1 (MQ=255)
                                                                     aaGGTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATc                         <  2:2289478/100‑1 (MQ=255)
                                                                       ggTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCaa                       <  1:682893/100‑1 (MQ=255)
                                                                        gTGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAg                      <  1:953522/100‑1 (MQ=255)
                                                                         tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGTTCTTGTTGGCATTCTTGCAATAGCCCtgaatgaaag                     >  1:3622795/1‑98 (MQ=255)
                                                                         tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                     >  1:3289846/1‑100 (MQ=255)
                                                                         tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                     <  1:2919909/100‑1 (MQ=255)
                                                                         tGACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                     <  1:1919621/100‑1 (MQ=255)
                                                                          gACGGTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGa                    >  2:3352160/1‑100 (MQ=255)
                                                                            cGGGGTCACGTTTTTCGGGGTAGGGCAGTGATCCGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                     <  2:2300234/97‑1 (MQ=255)
                                                                             ggTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                 >  2:2949037/1‑100 (MQ=255)
                                                                             ggTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                 <  1:2474205/100‑1 (MQ=255)
                                                                             ggTGTCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                 >  2:1955749/1‑100 (MQ=255)
                                                                              gtgtCACGTTTTTCGGGGTAGGGCAGTGATACGTTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTTCAATAGCCCTGAATCAAGGa                    <  2:1376898/96‑1 (MQ=255)
                                                                                gtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt              >  1:2136547/1‑100 (MQ=255)
                                                                                gtCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt              <  1:1315983/100‑1 (MQ=255)
                                                                                gtCACGTTTTTCGAGGTAGGGCAGGGATACGGTGGCGGGTTCGATACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAaga                 >  2:1993666/1‑97 (MQ=255)
                                                                                 tCACGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAtt             <  2:1334914/100‑1 (MQ=255)
                                                                                   aCGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGa           <  1:3620595/100‑1 (MQ=255)
                                                                                   aCGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGa           >  2:1883568/1‑100 (MQ=255)
                                                                                    cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          >  1:381501/1‑100 (MQ=255)
                                                                                    cGTTTTTCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc          >  2:683244/1‑100 (MQ=255)
                                                                                      tttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtg        >  2:3790763/1‑100 (MQ=255)
                                                                                      tttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtg        >  1:1933857/1‑100 (MQ=255)
                                                                                       ttttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgt       <  1:1833636/100‑1 (MQ=255)
                                                                                        tttCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtgtg      >  2:4031383/1‑100 (MQ=255)
                                                                                          tCGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGt    <  1:480062/100‑1 (MQ=255)
                                                                                           cGGGGTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt   >  1:2288824/1‑100 (MQ=255)
                                                                                            ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGtt   >  2:2703796/1‑99 (MQ=255)
                                                                                            ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtg  >  2:3637848/1‑100 (MQ=255)
                                                                                            ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtg  >  1:2002677/1‑100 (MQ=255)
                                                                                            ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtg  >  1:1565781/1‑100 (MQ=255)
                                                                                            ggggTAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtg  >  1:1265737/1‑100 (MQ=255)
                                                                                               |                                                                                                
ACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTG  >  NC_000913/1401749‑1401940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: