Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,833 G→C 16.7% intergenic (‑90/+21) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8330GC16.7% 326.1 / 57.5 125intergenic (‑90/+21)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (52/51);  new base C (14/7);  total (67/58)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

Only 100 of 127 total aligned reads displayed.

TTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTC  >  NC_000913/1441735‑1441932
                                                                                                  |                                                                                                   
 ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                   >  1:2515381/1‑100 (MQ=255)
 ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                   >  1:264191/1‑100 (MQ=255)
 ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                   >  1:4076511/1‑100 (MQ=255)
  tcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAt                                                                                                  >  1:172767/1‑100 (MQ=255)
   cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATg                                                                                                 >  1:3976767/1‑100 (MQ=255)
   cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATg                                                                                                 >  1:2794016/1‑100 (MQ=255)
      cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                              >  2:164776/1‑100 (MQ=255)
      cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                              >  1:1694449/1‑100 (MQ=255)
       aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                             >  1:3834704/1‑100 (MQ=255)
       aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAGGCAgg                                                                                             <  2:631415/100‑1 (MQ=255)
         tgtgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                           >  1:1786993/1‑100 (MQ=255)
           tgtttCTCAATTACAGTTTCTGACTTAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGa                                                                                          <  1:3266933/96‑1 (MQ=255)
            ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                        <  1:1598911/100‑1 (MQ=255)
            ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                        <  1:787603/100‑1 (MQ=255)
            ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                        <  2:897008/100‑1 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                      <  1:2953121/100‑1 (MQ=255)
              ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                      <  1:2633657/100‑1 (MQ=255)
               tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGc                                                                                     >  2:3408050/1‑100 (MQ=255)
                  cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                  <  1:3224542/100‑1 (MQ=255)
                  cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                  >  1:2572411/1‑100 (MQ=255)
                   aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                           >  2:3435137/1‑90 (MQ=255)
                   aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattca                                                                                 <  1:81822/100‑8 (MQ=37)
                    aTTACAGTTTCTTACTCTTGACTATTTTAAGAATAGAGGATGTAAGGTTATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattcag                                                                                <  2:3311698/100‑9 (MQ=21)
                    aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                <  1:86584/100‑1 (MQ=255)
                       aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                             <  2:1936645/100‑1 (MQ=255)
                       aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                             >  2:3541824/1‑100 (MQ=255)
                       aCAGTTTCTGACGCAGGACTATTTTAAGAATAGCGGCTGAAAGGTCACTGGGGGTTATCTGAATCATCTCCCCTGGAACGCAGGCGAACGGCa                                                                                    >  2:3541743/1‑93 (MQ=255)
                        cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                            <  2:680465/100‑1 (MQ=255)
                         aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                           <  1:4126487/100‑1 (MQ=255)
                         aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                           <  1:1164181/100‑1 (MQ=255)
                          gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                          >  2:290240/1‑100 (MQ=255)
                              cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                      <  1:4000831/100‑1 (MQ=255)
                               tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTACACCAgttc                                                                     >  2:2251147/1‑100 (MQ=255)
                                gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                    >  1:451668/1‑100 (MQ=255)
                                gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                    >  2:1004203/1‑100 (MQ=255)
                                gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                    >  2:25553/1‑100 (MQ=255)
                                 aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgt                                                                   >  2:276673/1‑100 (MQ=255)
                                    cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                <  2:941391/100‑1 (MQ=255)
                                    cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                <  2:4056816/100‑1 (MQ=255)
                                      ggACTATTTTCAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGCCTCCGCTCCCCTGGCATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCggg                                                              <  1:835547/100‑1 (MQ=255)
                                      ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTTgg                                                               >  2:3399507/1‑99 (MQ=255)
                                       gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                             <  1:1042628/100‑1 (MQ=255)
                                       gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                             <  1:3975354/100‑1 (MQ=255)
                                       gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                             <  2:1352355/100‑1 (MQ=255)
                                        aCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCa                                                            >  1:1808028/1‑100 (MQ=255)
                                          tATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                          <  1:3836287/100‑1 (MQ=255)
                                                aaGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                    <  1:1377670/100‑1 (MQ=255)
                                                 aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                   <  1:1499544/100‑1 (MQ=255)
                                                 aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                   <  1:3979248/100‑1 (MQ=255)
                                                  gAATAGAGGATGAAAGGTCATTGGGGATTATCTGAAGCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGAAGGTGTCGc                                                    >  2:3534214/1‑98 (MQ=255)
                                                      agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttt                                              <  1:2965106/100‑1 (MQ=255)
                                                        agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcn                                            <  1:2348598/100‑2 (MQ=255)
                                                        agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                            >  1:538137/1‑100 (MQ=255)
                                                         ggATGAACGGTGATTGGGGATTATCTGAATCCGCTCGCCTGGAATGCTGAGGGGCGGCGAGATTAAACCAGTTCGGGCGGTCAGGGGTCGCCGGTTTCCa                                           >  2:3901944/1‑100 (MQ=255)
                                                         ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTACGCCtttttgc                                            >  2:575742/1‑97 (MQ=255)
                                                            tGAAAGGTCATTTGGTATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGa                                         <  1:2424932/99‑1 (MQ=255)
                                                                   tCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                 >  1:2319754/1‑100 (MQ=255)
                                                                    cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                 >  2:1822698/1‑99 (MQ=255)
                                                                      ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                              >  1:2500919/1‑100 (MQ=255)
                                                                      ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                              <  2:2994393/100‑1 (MQ=255)
                                                                        ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                            >  1:3564618/1‑100 (MQ=255)
                                                                        ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                            >  2:907985/1‑100 (MQ=255)
                                                                        ggggATTATCTGAATCAGCTCCCCTGAAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                            >  1:2540839/1‑100 (MQ=255)
                                                                         gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                           <  1:25270/100‑1 (MQ=255)
                                                                          ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGTGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                           <  2:1743137/99‑1 (MQ=255)
                                                                          ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                          >  1:1075088/1‑100 (MQ=255)
                                                                           gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCa                         <  1:3020636/100‑1 (MQ=255)
                                                                             ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGc                       <  1:1178886/100‑1 (MQ=255)
                                                                               aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAATCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                     <  2:312716/100‑1 (MQ=255)
                                                                               aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                     <  1:1919870/100‑1 (MQ=255)
                                                                                tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                    >  1:2627110/1‑100 (MQ=255)
                                                                                tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTACAGATTGCTTAAGTTTTGCAGCGTa                    >  2:1016142/1‑100 (MQ=255)
                                                                                 cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                   <  2:3408844/100‑1 (MQ=255)
                                                                                 cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                   >  1:1445339/1‑100 (MQ=255)
                                                                                  tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                  >  2:4143906/1‑100 (MQ=255)
                                                                                  tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                  >  1:3317768/1‑100 (MQ=255)
                                                                                   cttgccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTc                 >  1:3200500/7‑100 (MQ=38)
                                                                                    ttgccgCTCCCCTGCATTCCAGGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCt                >  1:502221/6‑100 (MQ=37)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCATTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  1:2502114/3‑100 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTTTTTTTTTTTAAGTTTTGCAGCGTAg                   >  2:2880282/3‑94 (MQ=21)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGGTTAAGTTTTGCCGCGTCGTCTgag             >  2:121336/3‑100 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  1:2439772/3‑100 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  2:3551255/3‑100 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  1:929293/3‑100 (MQ=255)
                                                                                       ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             >  1:3340167/3‑100 (MQ=255)
                                                                                       cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTATGTTTTGCAGCGTAGTCTgag             <  2:2540824/100‑1 (MQ=255)
                                                                                       cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag             <  2:4116306/100‑1 (MQ=255)
                                                                                        cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  2:1167909/2‑100 (MQ=255)
                                                                                        cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGGTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  2:4088068/2‑100 (MQ=255)
                                                                                        aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGTGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            <  2:584369/100‑1 (MQ=255)
                                                                                        aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            >  1:1007009/1‑100 (MQ=255)
                                                                                         ggtacccGGGAATGTAGGTGAGCTGCAAGATTAAAGCAGTGCGTCCGGGCAGGTATCGCCTTTTTGCAGTTTGCTTAAGTTTTGCAGCGTATTCTGAGaa           <  2:1073715/96‑1 (MQ=255)
                                                                                         gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa           >  2:122139/1‑100 (MQ=255)
                                                                                         gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa           >  1:1025751/1‑100 (MQ=255)
                                                                                          cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa          >  1:2898178/1‑100 (MQ=255)
                                                                                           tCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga            <  2:3945339/97‑1 (MQ=255)
                                                                                             cccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc       <  1:3191501/100‑1 (MQ=255)
                                                                                                tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg    >  1:2554361/1‑100 (MQ=255)
                                                                                                  gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTCAGATTTCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGt   >  2:375122/1‑99 (MQ=255)
                                                                                                  gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTc  >  2:2985281/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTC  >  NC_000913/1441735‑1441932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: