Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,835 A→T 17.1% intergenic (‑92/+19) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8350AT17.1% 300.8 / 53.2 123intergenic (‑92/+19)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (51/51);  new base T (14/7);  total (65/58)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01

Only 100 of 128 total aligned reads displayed.

TTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAG  >  NC_000913/1441736‑1441934
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ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                     >  1:264191/1‑100 (MQ=255)
ttcttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGaa                                                                                                     >  1:2515381/1‑100 (MQ=255)
  cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATg                                                                                                   >  1:2794016/1‑100 (MQ=255)
     cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                >  2:164776/1‑100 (MQ=255)
     cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                >  1:1694449/1‑100 (MQ=255)
      aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                               >  1:3834704/1‑100 (MQ=255)
      aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAGGCAgg                                                                                               <  2:631415/100‑1 (MQ=255)
       ttGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAggg                                                                                              >  2:1097076/1‑100 (MQ=255)
        tgtgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                             >  1:1786993/1‑100 (MQ=255)
           ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                          <  1:787603/100‑1 (MQ=255)
           ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                          <  2:897008/100‑1 (MQ=255)
           ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                          <  1:1598911/100‑1 (MQ=255)
             ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                        <  1:2633657/100‑1 (MQ=255)
             ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                        <  1:2953121/100‑1 (MQ=255)
              tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGc                                                                                       >  2:3408050/1‑100 (MQ=255)
                 cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                    <  1:3224542/100‑1 (MQ=255)
                 cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                    >  1:2572411/1‑100 (MQ=255)
                  aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                             >  2:3435137/1‑90 (MQ=255)
                  aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                   <  1:1785412/100‑1 (MQ=255)
                  aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattca                                                                                   <  1:81822/100‑8 (MQ=37)
                   aTTACAGTTTCTTACTCTTGACTATTTTAAGAATAGAGGATGTAAGGTTATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattcag                                                                                  <  2:3311698/100‑9 (MQ=21)
                   aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                  >  2:1880041/1‑100 (MQ=255)
                   aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                  <  1:86584/100‑1 (MQ=255)
                     tACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTa                                                                                >  2:4031069/1‑100 (MQ=255)
                      aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                               >  2:3541824/1‑100 (MQ=255)
                      aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                               <  2:1936645/100‑1 (MQ=255)
                      aCAGTTTCTGACGCAGGACTATTTTAAGAATAGCGGCTGAAAGGTCACTGGGGGTTATCTGAATCATCTCCCCTGGAACGCAGGCGAACGGCa                                                                                      >  2:3541743/1‑93 (MQ=255)
                       cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                              <  2:680465/100‑1 (MQ=255)
                       cAGTTTCTGACTCAGGAATATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGACTCAGCTCCCCTGGAATGCAGGGGAGCTGCAACATTaaa                                                                              >  2:1252901/1‑100 (MQ=255)
                        aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                             <  1:4126487/100‑1 (MQ=255)
                        aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                             <  1:1164181/100‑1 (MQ=255)
                        aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                             >  1:910410/1‑100 (MQ=255)
                         gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                            >  2:290240/1‑100 (MQ=255)
                         gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                            <  1:440444/100‑1 (MQ=255)
                           ttCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAg                                                                          <  1:554898/100‑1 (MQ=255)
                             cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                        <  1:4000831/100‑1 (MQ=255)
                              tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTACACCAgttc                                                                       >  2:2251147/1‑100 (MQ=255)
                               gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                      >  1:451668/1‑100 (MQ=255)
                               gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                      >  2:25553/1‑100 (MQ=255)
                               gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                      >  2:1004203/1‑100 (MQ=255)
                                   cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                  <  2:941391/100‑1 (MQ=255)
                                      gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                               <  2:1352355/100‑1 (MQ=255)
                                      gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                               <  1:1042628/100‑1 (MQ=255)
                                      gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                               <  1:3975354/100‑1 (MQ=255)
                                       aCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCa                                                              >  1:1808028/1‑100 (MQ=255)
                                         tATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                            <  1:3836287/100‑1 (MQ=255)
                                          aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAg                                                             >  2:3329067/1‑98 (MQ=255)
                                               aaGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                      <  1:1377670/100‑1 (MQ=255)
                                                aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     <  1:3979248/100‑1 (MQ=255)
                                                aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                     <  1:1499544/100‑1 (MQ=255)
                                                   aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttt                                                  >  1:3978239/1‑100 (MQ=255)
                                                     agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttt                                                <  1:2965106/100‑1 (MQ=255)
                                                       agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcn                                              <  1:2348598/100‑2 (MQ=255)
                                                       agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                              <  2:275063/100‑1 (MQ=255)
                                                       agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                              >  1:538137/1‑100 (MQ=255)
                                                        ggATGAACGGTGATTGGGGATTATCTGAATCCGCTCGCCTGGAATGCTGAGGGGCGGCGAGATTAAACCAGTTCGGGCGGTCAGGGGTCGCCGGTTTCCa                                             >  2:3901944/1‑100 (MQ=255)
                                                        ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTACGCCtttttgc                                              >  2:575742/1‑97 (MQ=255)
                                                           tGAAAGGTCATTTGGTATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGa                                           <  1:2424932/99‑1 (MQ=255)
                                                                   cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                   >  2:1822698/1‑99 (MQ=255)
                                                                     ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                                >  1:2500919/1‑100 (MQ=255)
                                                                     ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                                <  2:2994393/100‑1 (MQ=255)
                                                                      tGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGttt                               <  2:3502298/100‑1 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                              >  2:907985/1‑100 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                              >  1:3564618/1‑100 (MQ=255)
                                                                       ggggATTATCTGAATCAGCTCCCCTGAAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                              >  1:2540839/1‑100 (MQ=255)
                                                                        gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                             <  1:25270/100‑1 (MQ=255)
                                                                         ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                            >  1:1075088/1‑100 (MQ=255)
                                                                          gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCa                           <  1:3020636/100‑1 (MQ=255)
                                                                            ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGc                         <  1:1178886/100‑1 (MQ=255)
                                                                              aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAATCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                       <  2:312716/100‑1 (MQ=255)
                                                                              aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                       <  1:1919870/100‑1 (MQ=255)
                                                                               tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                      >  1:2627110/1‑100 (MQ=255)
                                                                                cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                     >  1:1445339/1‑100 (MQ=255)
                                                                                cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                     <  2:3408844/100‑1 (MQ=255)
                                                                                 tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                    >  2:4143906/1‑100 (MQ=255)
                                                                                 tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                    >  1:3317768/1‑100 (MQ=255)
                                                                                  cttgccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTc                   >  1:3200500/7‑100 (MQ=38)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCATTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:2502114/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTTTTTTTTTTTAAGTTTTGCAGCGTAg                     >  2:2880282/3‑94 (MQ=21)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:2439772/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  2:3551255/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:929293/3‑100 (MQ=255)
                                                                                      ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               >  1:3340167/3‑100 (MQ=255)
                                                                                      cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTATGTTTTGCAGCGTAGTCTgag               <  2:2540824/100‑1 (MQ=255)
                                                                                      cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag               <  2:4116306/100‑1 (MQ=255)
                                                                                       cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              >  2:1167909/2‑100 (MQ=255)
                                                                                       cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGGTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              >  2:4088068/2‑100 (MQ=255)
                                                                                       aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGTGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              <  2:584369/100‑1 (MQ=255)
                                                                                       aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              >  1:1007009/1‑100 (MQ=255)
                                                                                        ggtacccGGGAATGTAGGTGAGCTGCAAGATTAAAGCAGTGCGTCCGGGCAGGTATCGCCTTTTTGCAGTTTGCTTAAGTTTTGCAGCGTATTCTGAGaa             <  2:1073715/96‑1 (MQ=255)
                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa             >  1:1025751/1‑100 (MQ=255)
                                                                                        gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa             >  2:122139/1‑100 (MQ=255)
                                                                                         cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa            >  1:2898178/1‑100 (MQ=255)
                                                                                          tCCCCTGGAATGCGGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt           <  1:3275312/100‑1 (MQ=255)
                                                                                          tCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga              <  2:3945339/97‑1 (MQ=255)
                                                                                            cccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc         <  1:3191501/100‑1 (MQ=255)
                                                                                               tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg      >  1:2554361/1‑100 (MQ=255)
                                                                                                 gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTCAGATTTCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGt     >  2:375122/1‑99 (MQ=255)
                                                                                                 gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTc    >  2:2985281/1‑100 (MQ=255)
                                                                                                   aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCag  >  1:4070321/1‑100 (MQ=255)
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TTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAG  >  NC_000913/1441736‑1441934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: