Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,837 G→C 17.1% intergenic (‑94/+17) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8370GC17.1% 317.7 / 58.6 123intergenic (‑94/+17)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (50/52);  new base C (14/7);  total (64/59)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 126 total aligned reads displayed.

CTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAG  >  NC_000913/1441738‑1441936
                                                                                                   |                                                                                                   
cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATg                                                                                                     >  1:3976767/1‑100 (MQ=255)
   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                  >  2:164776/1‑100 (MQ=255)
   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                  >  1:1694449/1‑100 (MQ=255)
    aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgg                                                                                                 >  1:3834704/1‑100 (MQ=255)
    aTTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAAGGCAgg                                                                                                 <  2:631415/100‑1 (MQ=255)
         ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                            <  2:897008/100‑1 (MQ=255)
         ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                            <  1:787603/100‑1 (MQ=255)
         ggTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                            <  1:1598911/100‑1 (MQ=255)
           ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                          <  1:2953121/100‑1 (MQ=255)
           ttCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                          <  1:2633657/100‑1 (MQ=255)
            tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGc                                                                                         >  2:3408050/1‑100 (MQ=255)
               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                      <  1:3224542/100‑1 (MQ=255)
                aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGa                                                                                     <  1:1785412/100‑1 (MQ=255)
                aaTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGctgattca                                                                                     <  1:81822/100‑8 (MQ=37)
                 aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                    >  2:1880041/1‑100 (MQ=255)
                 aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                    <  1:86584/100‑1 (MQ=255)
                    aCAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaa                                                                                 >  2:3541824/1‑100 (MQ=255)
                    aCAGTTTCTGACGCAGGACTATTTTAAGAATAGCGGCTGAAAGGTCACTGGGGGTTATCTGAATCATCTCCCCTGGAACGCAGGCGAACGGCa                                                                                        >  2:3541743/1‑93 (MQ=255)
                     cAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTaaa                                                                                <  2:680465/100‑1 (MQ=255)
                     cAGTTTCTGACTCAGGAATATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGACTCAGCTCCCCTGGAATGCAGGGGAGCTGCAACATTaaa                                                                                >  2:1252901/1‑100 (MQ=255)
                      aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                               <  1:1164181/100‑1 (MQ=255)
                      aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                               <  1:4126487/100‑1 (MQ=255)
                      aGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                               <  1:2533432/100‑1 (MQ=255)
                       gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGTATTATCTGAATCAGCTCGCCTGGAATGCAGGGTAGCGGCAAGATTaaa                                                                                >  2:355713/1‑98 (MQ=255)
                       gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAcc                                                                              >  2:290240/1‑100 (MQ=255)
                           cTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                          <  1:4000831/100‑1 (MQ=255)
                            tGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTACACCAgttc                                                                         >  2:2251147/1‑100 (MQ=255)
                             gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                        >  1:451668/1‑100 (MQ=255)
                             gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                        >  2:1004203/1‑100 (MQ=255)
                             gACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                        >  2:25553/1‑100 (MQ=255)
                              aCTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgt                                                                       >  2:276673/1‑100 (MQ=255)
                                 cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                    <  2:4056816/100‑1 (MQ=255)
                                 cAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGttcgttcg                                                                    <  2:941391/100‑1 (MQ=255)
                                   ggACTATTTTCAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGCCTCCGCTCCCCTGGCATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCggg                                                                  <  1:835547/100‑1 (MQ=255)
                                   ggACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTTgg                                                                   >  2:3399507/1‑99 (MQ=255)
                                    gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                                 <  1:1042628/100‑1 (MQ=255)
                                    gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGc                                                                 <  1:3975354/100‑1 (MQ=255)
                                     aCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCa                                                                >  1:1808028/1‑100 (MQ=255)
                                       tATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAgg                                                              <  1:3836287/100‑1 (MQ=255)
                                        aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAg                                                               >  2:3329067/1‑98 (MQ=255)
                                             aaGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGc                                                        <  1:1377670/100‑1 (MQ=255)
                                              aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                       <  1:3979248/100‑1 (MQ=255)
                                              aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                       <  1:1499544/100‑1 (MQ=255)
                                               gAATAGAGGATGAAAGGTCATTGGGGATTATCTGAAGCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGAAGGTGTCGc                                                        >  2:3534214/1‑98 (MQ=255)
                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCttttt                                                  <  1:2965106/100‑1 (MQ=255)
                                                     agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcn                                                <  1:2348598/100‑2 (MQ=255)
                                                     agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                <  2:275063/100‑1 (MQ=255)
                                                     agGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                >  1:538137/1‑100 (MQ=255)
                                                      ggATGAACGGTGATTGGGGATTATCTGAATCCGCTCGCCTGGAATGCTGAGGGGCGGCGAGATTAAACCAGTTCGGGCGGTCAGGGGTCGCCGGTTTCCa                                               >  2:3901944/1‑100 (MQ=255)
                                                      ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTACGCCtttttgc                                                >  2:575742/1‑97 (MQ=255)
                                                               gTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTa                                      <  1:2469807/100‑1 (MQ=255)
                                                                tCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                     >  1:2319754/1‑100 (MQ=255)
                                                                 cATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTaa                                     >  2:1822698/1‑99 (MQ=255)
                                                                   ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                                  <  2:2994393/100‑1 (MQ=255)
                                                                   ttGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                                  >  1:2500919/1‑100 (MQ=255)
                                                                    tGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGttt                                 <  2:3502298/100‑1 (MQ=255)
                                                                     ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                >  2:907985/1‑100 (MQ=255)
                                                                     ggggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                >  1:3564618/1‑100 (MQ=255)
                                                                     ggggATTATCTGAATCAGCTCCCCTGAAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtttt                                >  1:2540839/1‑100 (MQ=255)
                                                                      gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                               <  1:25270/100‑1 (MQ=255)
                                                                       ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGTGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTg                               <  2:1743137/99‑1 (MQ=255)
                                                                       ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                              >  1:1075088/1‑100 (MQ=255)
                                                                       ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                              <  2:2988777/100‑1 (MQ=255)
                                                                          ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGc                           <  1:1178886/100‑1 (MQ=255)
                                                                            aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAATCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                         <  2:312716/100‑1 (MQ=255)
                                                                            aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGt                         <  1:1919870/100‑1 (MQ=255)
                                                                             tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTa                        >  1:2627110/1‑100 (MQ=255)
                                                                             tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTACAGATTGCTTAAGTTTTGCAGCGTa                        >  2:1016142/1‑100 (MQ=255)
                                                                              cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                       <  2:3408844/100‑1 (MQ=255)
                                                                              cTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAg                       >  1:1445339/1‑100 (MQ=255)
                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                      >  1:3317768/1‑100 (MQ=255)
                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGt                      >  2:4143906/1‑100 (MQ=255)
                                                                                cttgccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTc                     >  1:3200500/7‑100 (MQ=38)
                                                                                 ttgccgCTCCCCTGCATTCCAGGGGAGCTGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCt                    >  1:502221/6‑100 (MQ=37)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCATTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 >  1:2502114/3‑100 (MQ=255)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTTTTTTTTTTTAAGTTTTGCAGCGTAg                       >  2:2880282/3‑94 (MQ=21)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGGTTAAGTTTTGCCGCGTCGTCTgag                 >  2:121336/3‑100 (MQ=255)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 >  1:2439772/3‑100 (MQ=255)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 >  1:3340167/3‑100 (MQ=255)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 >  1:3159798/3‑100 (MQ=255)
                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 >  1:929293/3‑100 (MQ=255)
                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTATGTTTTGCAGCGTAGTCTgag                 <  2:2540824/100‑1 (MQ=255)
                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgag                 <  2:4116306/100‑1 (MQ=255)
                                                                                     cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                >  2:1167909/2‑100 (MQ=255)
                                                                                     cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGGTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                >  2:4088068/2‑100 (MQ=255)
                                                                                     aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGTGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                <  2:584369/100‑1 (MQ=255)
                                                                                     aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                <  1:2575514/100‑1 (MQ=255)
                                                                                     aGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                >  1:1007009/1‑100 (MQ=255)
                                                                                      ggtacccGGGAATGTAGGTGAGCTGCAAGATTAAAGCAGTGCGTCCGGGCAGGTATCGCCTTTTTGCAGTTTGCTTAAGTTTTGCAGCGTATTCTGAGaa               <  2:1073715/96‑1 (MQ=255)
                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa               >  1:1025751/1‑100 (MQ=255)
                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa               >  2:122139/1‑100 (MQ=255)
                                                                                       cTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaaa              >  1:2898178/1‑100 (MQ=255)
                                                                                        tCCCCTGGAATGCGGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt             <  1:3275312/100‑1 (MQ=255)
                                                                                        tCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTgaga                <  2:3945339/97‑1 (MQ=255)
                                                                                          cccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc           <  1:3191501/100‑1 (MQ=255)
                                                                                          cccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATAc           <  1:281746/100‑1 (MQ=255)
                                                                                             tGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg        >  1:2554361/1‑100 (MQ=255)
                                                                                               gAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTTCAGATTTCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGt       >  2:375122/1‑99 (MQ=255)
                                                                                                 aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGTTTGCTTAAGTTTTGCTGCGTAGTCTGATAAATACTTGTCag    <  2:1940435/100‑1 (MQ=255)
                                                                                                 aTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCag    >  1:4070321/1‑100 (MQ=255)
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CTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAG  >  NC_000913/1441738‑1441936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: