Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,460,438 T→C 15.4% L462S (TTG→TCG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,460,4380TC15.4% 296.0 / 42.7 129L462S (TTG→TCG) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base T (53/56);  new base C (13/7);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 134 total aligned reads displayed.

CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTG  >  NC_000913/1460341‑1460537
                                                                                                 |                                                                                                   
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTgcgc                                                                                              <  1:3057719/100‑1 (MQ=255)
     cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTgcgc                                                                                              >  2:3617707/1‑100 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTgcgc                                                                                              <  2:302295/99‑1 (MQ=255)
      tGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGcc                                                                                             <  1:965404/100‑1 (MQ=255)
       ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCa                                                                                            >  2:897531/1‑100 (MQ=255)
       ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCa                                                                                            >  1:26515/1‑100 (MQ=255)
         ngAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCaa                                                                                           >  2:850661/2‑99 (MQ=255)
            gcgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACggg                                                                                       <  1:340096/100‑1 (MQ=255)
            gcgcAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACggg                                                                                       >  1:2248510/1‑100 (MQ=255)
             cgcAAGTGGGCTGGCAGGGAATATTAAGGTGCCTTGAAAAGCGACAGGATGACTTTGGCGAAGAGCGCTAGCGGCCATGTTGATTGCTGCGCCAACGGgc                                                                                      <  2:177484/100‑1 (MQ=255)
             cgcAACTTGGCTGGCAGCGAATCTTAAGGCTCCTTGAGAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGgc                                                                                      <  2:2814160/100‑1 (MQ=255)
                aaCTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCt                                                                                   <  2:49099/100‑1 (MQ=255)
                  cTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTc                                                                                 <  1:3289658/100‑1 (MQ=255)
                  cTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTc                                                                                 <  2:1205706/100‑1 (MQ=255)
                     ggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTgg                                                                              >  2:1755501/1‑100 (MQ=255)
                      gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGga                                                                             <  2:1692203/100‑1 (MQ=255)
                       ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgag                                                                            <  2:2059186/100‑1 (MQ=255)
                       ctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgag                                                                            <  1:855950/100‑1 (MQ=255)
                        tggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGATCGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTgg                                                                              <  2:4006985/97‑1 (MQ=255)
                        tggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGgaga                                                                           <  1:2836740/100‑1 (MQ=255)
                         ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGagag                                                                          >  2:4174845/1‑100 (MQ=255)
                          gcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGTCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGc                                                                         >  2:3743863/1‑100 (MQ=255)
                           cAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCg                                                                        >  1:2372655/1‑100 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCgg                                                                       <  1:4002271/100‑1 (MQ=255)
                            aGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCg                                                                        <  2:2806112/99‑1 (MQ=255)
                             gCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGt                                                                      >  2:2398458/1‑100 (MQ=255)
                               gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTa                                                                    <  2:3505535/100‑1 (MQ=255)
                                aaTATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTAt                                                                   >  1:1298074/1‑100 (MQ=255)
                                  tatTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGa                                                                 >  1:2878925/1‑100 (MQ=255)
                                   atTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAg                                                                >  1:123542/1‑100 (MQ=255)
                                    ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGt                                                               <  1:1956772/100‑1 (MQ=255)
                                    ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGt                                                               <  1:4132574/100‑1 (MQ=255)
                                     tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGtn                                                              <  1:1886712/100‑2 (MQ=255)
                                     tAAGGCTCCCTGAAAAGCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGt                                                               <  2:3490838/99‑1 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCa                                                             >  2:3835030/1‑100 (MQ=255)
                                      aaGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGCGCGCTTCTGGAGAGCGGTTATGAGTCa                                                             >  2:3361210/1‑100 (MQ=255)
                                       aGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCAt                                                            <  1:1089428/100‑1 (MQ=255)
                                        ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATa                                                           <  2:4081672/100‑1 (MQ=255)
                                         gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATaa                                                          >  2:4171877/1‑100 (MQ=255)
                                          cTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAg                                                         <  1:4069611/100‑1 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTTCTCCAACGGGCGCTTCTGGAGAGCGGTTATGATTCATAAgg                                                        >  1:614404/1‑100 (MQ=255)
                                           tCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAgg                                                        >  1:1837714/1‑100 (MQ=255)
                                               tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTg                                                    >  1:211579/1‑100 (MQ=255)
                                                gaaatgcTACAGCATCACTATGGCGAAGAACGCTATCGTCCATGTTCATTGCGGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGCGTCATAAGGCCTg                                                    <  2:783545/93‑1 (MQ=255)
                                                gAAAATCTACAGCATTACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                                   >  2:2659424/1‑100 (MQ=255)
                                                gAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTgg                                                   <  2:3304389/100‑1 (MQ=255)
                                                 aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGc                                                  <  1:283466/100‑1 (MQ=255)
                                                 aaaaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGc                                                  <  1:341201/100‑1 (MQ=255)
                                                   aaTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaa                                                >  2:2400679/1‑100 (MQ=255)
                                                     tCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaaa                                              >  1:1100981/1‑100 (MQ=255)
                                                     tCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaaa                                              >  1:1097884/1‑100 (MQ=255)
                                                     tCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCaaaa                                              <  2:3827064/100‑1 (MQ=255)
                                                      ntACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                             >  2:927867/2‑100 (MQ=255)
                                                      cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                             <  1:216521/100‑1 (MQ=255)
                                                      cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                             <  1:1699542/100‑1 (MQ=255)
                                                      cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAAt                                             >  1:1515448/1‑100 (MQ=255)
                                                       tACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATg                                            >  1:334887/1‑100 (MQ=255)
                                                        aCAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGc                                           <  1:2246105/100‑1 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                        <  1:1860431/100‑1 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                        >  1:3413989/1‑100 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                        <  1:377266/100‑1 (MQ=255)
                                                           gCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCa                                        >  2:1122710/1‑100 (MQ=255)
                                                            cATCTCTATGGCGAAGAGCGCTAGCGCGCATGTTTATTGTTGCGCGGACGGGCGCTTATGGAGAGCGGTGATGAGTTATAAAGCCTGGCAAAATGcc                                          <  2:3980080/97‑1 (MQ=255)
                                                            cATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCAt                                       >  1:677783/1‑100 (MQ=255)
                                                             aTCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATg                                      <  1:1789392/100‑1 (MQ=255)
                                                              tCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGc                                     <  2:2794526/100‑1 (MQ=255)
                                                               cACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                    <  1:3861028/100‑1 (MQ=255)
                                                               cACTATGGCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                    >  2:852175/1‑100 (MQ=255)
                                                                aCTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAACGCCCATGCaa                                   >  1:2648450/1‑100 (MQ=255)
                                                                 cTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                                  >  2:232027/1‑100 (MQ=255)
                                                                 cTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAt                                  >  2:3039973/1‑100 (MQ=255)
                                                                   aTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                                >  2:3083890/1‑100 (MQ=255)
                                                                   aTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                                <  2:3635030/100‑1 (MQ=255)
                                                                    tGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                               <  1:1517719/100‑1 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                             >  2:2552311/1‑100 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                             >  2:2602397/1‑100 (MQ=255)
                                                                      gCGAAGAACGCTATCGCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                             >  1:3692599/1‑100 (MQ=255)
                                                                       cGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATga                            <  1:2472027/100‑1 (MQ=255)
                                                                       cGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATga                            <  2:1919584/100‑1 (MQ=255)
                                                                        gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                           <  1:850666/100‑1 (MQ=255)
                                                                        gaagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                           <  1:2475397/100‑1 (MQ=255)
                                                                               gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg                    <  2:111225/100‑1 (MQ=255)
                                                                               gCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg                    <  1:1489403/100‑1 (MQ=255)
                                                                                cTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGc                   >  1:3652198/1‑100 (MQ=255)
                                                                                 tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc                  <  2:3914339/100‑1 (MQ=255)
                                                                                 tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc                  >  1:3679044/1‑100 (MQ=255)
                                                                                  aTCGCCCACGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCt                 >  2:2626257/1‑100 (MQ=255)
                                                                                    cGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGCGCGGTTATGAGTCATAAGGCCTGGCAAAATACCCATGCAATGTATGAGAACGATGCCTg                >  2:2516579/1‑99 (MQ=255)
                                                                                    cGCCCAGGTTCATTGCTGCACCAAAGGGCGCTTCTGGAGAGCGGATATGTGTCATAAGACCTGGAAAAAAGCACATGCAATATATGAGAACAAt                     >  2:251931/1‑94 (MQ=255)
                                                                                     gCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg              >  1:2682762/1‑100 (MQ=255)
                                                                                     gCCCATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcg              <  1:3136888/100‑1 (MQ=255)
                                                                                      cccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc             >  1:11790/1‑100 (MQ=255)
                                                                                      cccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc             <  1:3762233/100‑1 (MQ=255)
                                                                                      cccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgcgc             >  2:3746880/1‑100 (MQ=255)
                                                                                       ccATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGcc            <  2:1855356/100‑1 (MQ=255)
                                                                                        cATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa           >  1:2046109/1‑100 (MQ=255)
                                                                                        cATGTTCATCGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa           >  1:2261632/1‑100 (MQ=255)
                                                                                              cATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcgc     >  1:3216124/1‑100 (MQ=255)
                                                                                              cATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGAGCCAAAgcgc     >  2:3257483/1‑100 (MQ=255)
                                                                                                ttGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCtt   <  1:2961613/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTG  >  NC_000913/1460341‑1460537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: