Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,515,267 A→T 73.7% D169V (GAT→GTT)  ydcV → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,515,2670AT73.7% 204.2 / 93.1 130D169V (GAT→GTT) ydcVputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (19/15);  new base T (57/38);  total (76/54)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

Only 100 of 132 total aligned reads displayed.

TCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTC  >  NC_000913/1515168‑1515363
                                                                                                   |                                                                                                
tCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGa                                                                                                  <  2:2408134/100‑1 (MQ=255)
      tcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTgg                                                                                            >  2:2723243/1‑100 (MQ=255)
        ggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTTGTTGAGGCGTCAATGGATCTTgggg                                                                                          <  1:1939396/100‑1 (MQ=255)
        ggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTgggg                                                                                          <  1:1047529/100‑1 (MQ=255)
         gtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGc                                                                                         <  1:892814/100‑1 (MQ=255)
           cATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCa                                                                                       >  1:3727455/1‑100 (MQ=255)
           cATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCa                                                                                       >  2:529604/1‑100 (MQ=255)
            aTGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCaa                                                                                      >  2:2364858/1‑100 (MQ=255)
             tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAt                                                                                     >  2:865452/1‑100 (MQ=255)
             tGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCaa                                                                                      >  2:3900580/1‑99 (MQ=255)
              gCGAGTTTTTGTGTAGTTGTGGTTTTGAACTTTGTCGTTGCGCGTTTGCGTCGCACCTCCTGCAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCaa                                                                                      <  2:3633337/98‑1 (MQ=255)
                gtcTTTTTGTGTAATTGTGGTGTTTAACAATGTCATCGCCCGATTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggc                                                                                  >  1:2821393/3‑100 (MQ=255)
                gACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtggc                                                                                  >  2:3598802/1‑100 (MQ=255)
                gACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtggc                                                                                  >  2:3093603/1‑100 (MQ=255)
                gACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggc                                                                                  <  1:476945/100‑1 (MQ=255)
                  cTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAAtggctg                                                                                >  1:2157875/1‑100 (MQ=255)
                  cTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggctg                                                                                <  2:2799345/100‑1 (MQ=255)
                   tttttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAAtggctgg                                                                               >  1:4083740/1‑100 (MQ=255)
                      ttGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCaa                                                                            >  1:1906123/1‑100 (MQ=255)
                        gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAAc                                                                          >  1:3887747/1‑100 (MQ=255)
                        gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAAc                                                                          >  1:2848755/1‑100 (MQ=255)
                          gtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCt                                                                        >  1:1185099/1‑100 (MQ=255)
                           tAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCtt                                                                       <  1:1439613/100‑1 (MQ=255)
                            aGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTc                                                                      >  1:3722920/1‑100 (MQ=255)
                             gTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTcc                                                                     <  1:1018344/100‑1 (MQ=255)
                              ttGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCg                                                                    <  1:137107/100‑1 (MQ=255)
                               tgtgGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGc                                                                   <  1:789908/100‑1 (MQ=255)
                               tgtgGTGTTTAACAATGTCATCACCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGc                                                                   <  1:3843350/100‑1 (MQ=255)
                                gtggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGCGGCCAATAGCTGGCAAACCTTCCGCt                                                                  >  2:3880907/1‑100 (MQ=255)
                                 tggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTa                                                                 >  2:1818668/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                >  2:3459023/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                >  1:3759936/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTAc                                                                >  2:3413271/1‑100 (MQ=255)
                                  ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGGTGAGGCGTCAATGGTTCTTCGGGCCAATGGCTGGCAGACCTTCCGCTAc                                                                >  2:1922951/1‑100 (MQ=255)
                                   gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACg                                                               >  1:1953838/1‑100 (MQ=255)
                                   gtgtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACg                                                               >  1:429826/1‑100 (MQ=255)
                                     gtTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCGGGTTGAGGTGTCAATGGTTCTTGGGGCCAATGGCTGGCAATCCTTCCGCTACGt                                                              <  1:2403983/99‑1 (MQ=255)
                                       ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgt                                                           >  1:1601402/1‑100 (MQ=255)
                                        tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgtg                                                          >  2:3345459/1‑100 (MQ=255)
                                        tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgtg                                                          >  1:400497/1‑100 (MQ=255)
                                        tAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgtg                                                          >  1:416653/1‑100 (MQ=255)
                                         aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCCATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgtgt                                                         >  2:1428816/1‑100 (MQ=255)
                                         aaCAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAgtgt                                                         <  1:649882/100‑1 (MQ=255)
                                           cAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTg                                                       >  1:3029425/1‑100 (MQ=255)
                                            aaTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGc                                                      >  2:1223471/1‑100 (MQ=255)
                                             aTGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGcc                                                     >  1:1084244/1‑100 (MQ=255)
                                                tCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGaa                                                  <  1:1609840/100‑1 (MQ=255)
                                                 cATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAt                                                 >  2:1696670/1‑100 (MQ=255)
                                                   tCGCCCGTGTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAtct                                               <  2:1116962/100‑1 (MQ=255)
                                                    cGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGACGTCAATGGTACTTGGGGCAAATGACTGGCAAACCATCCGCTACGTAGTGTTGCCGAAtctc                                              >  2:3533142/1‑100 (MQ=255)
                                                     gCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAAtctc                                              >  1:2413323/1‑99 (MQ=255)
                                                     gCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCa                                             >  2:3488260/1‑100 (MQ=255)
                                                      cccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAg                                            >  1:2981796/1‑100 (MQ=255)
                                                       ccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGt                                           >  1:199336/1‑100 (MQ=255)
                                                       ccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGt                                           >  2:3435537/1‑100 (MQ=255)
                                                       ccGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGt                                           <  2:3727428/100‑1 (MQ=255)
                                                           tttCGCCGCACCTCCTGGAGTCTGGTTGAGTCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCgg                                       <  2:4150589/100‑1 (MQ=255)
                                                           tttCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCgg                                       >  2:729124/1‑100 (MQ=255)
                                                             tCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCg                                     <  1:2797183/100‑1 (MQ=255)
                                                             tCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCg                                     >  2:3446816/1‑100 (MQ=255)
                                                             tCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCg                                     <  1:3951353/100‑1 (MQ=255)
                                                              cgccgcACCTCCTGCAGGCTGGTTGAGGCGTCAATGGTCCTTGCGGCCAATGGCTGTCAAACCTTCCGCGACGGAGTGTGGCCGAATCTCAGGTCGGc                                      >  2:1384170/1‑98 (MQ=255)
                                                               gccgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGtt                                   >  1:2991141/1‑100 (MQ=255)
                                                                 cgcACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTAc                                 <  1:1440995/100‑1 (MQ=255)
                                                                   cACCTCCTGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTg                               <  1:3983270/100‑1 (MQ=255)
                                                                   cACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTg                               <  1:3452987/100‑1 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                             >  1:8273/1‑100 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                             <  2:3722893/100‑1 (MQ=255)
                                                                     cctcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGc                             <  2:3665252/100‑1 (MQ=255)
                                                                       tcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCag                           >  2:809050/1‑100 (MQ=255)
                                                                       tcctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCag                           >  1:3127483/1‑100 (MQ=255)
                                                                        cctGGAGTCTTGTTGAGGCGCCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagg                          <  2:2488059/100‑1 (MQ=255)
                                                                        cctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagg                          >  2:4066524/1‑100 (MQ=255)
                                                                        cctGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagg                          >  2:4121407/1‑100 (MQ=255)
                                                                        cctGGAGTCTGGTTGAGGCGTCAATGGTTCGTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCagg                          <  2:3534458/100‑1 (MQ=255)
                                                                          tGGAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggag                        >  2:2972247/1‑100 (MQ=255)
                                                                           ggAGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCaggagg                       <  2:2533343/100‑1 (MQ=255)
                                                                             aGTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAggagga                      <  2:1740180/99‑1 (MQ=255)
                                                                              gTCTGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAAt                    >  2:3989260/1‑100 (MQ=255)
                                                                                 tGGTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                 <  2:3858224/100‑1 (MQ=255)
                                                                                 tGGTTGAGGCGTCAATGGTTCTTGGGCCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                 >  1:2531193/1‑100 (MQ=255)
                                                                                 tGGTTGAGGCGTCAATGGTTCTTGGGCCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                 >  1:2531426/1‑100 (MQ=255)
                                                                                 tGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCt                 >  1:461929/1‑100 (MQ=255)
                                                                                  ggTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTg                >  2:2127786/1‑100 (MQ=255)
                                                                                   gTTGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg               >  2:162150/1‑100 (MQ=255)
                                                                                   gTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTgg               <  2:1906113/100‑1 (MQ=255)
                                                                                    ttGAGGCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGc              <  1:3598829/100‑1 (MQ=255)
                                                                                    ttGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGc              <  1:1320381/100‑1 (MQ=255)
                                                                                      gAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt            <  2:3280186/100‑1 (MQ=255)
                                                                                       aGGCGGCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAGGGCTGGCg             >  2:4172794/1‑98 (MQ=255)
                                                                                        ggCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGttt          >  1:1507187/1‑100 (MQ=255)
                                                                                         gCGTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTg         <  2:2329540/100‑1 (MQ=255)
                                                                                          cGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGt            <  2:3282853/96‑1 (MQ=255)
                                                                                           gtgaaTGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc       <  1:1133396/97‑1 (MQ=255)
                                                                                           gTCAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc       >  1:2509423/1‑100 (MQ=255)
                                                                                           gTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGcc       <  1:3852899/100‑1 (MQ=255)
                                                                                            tCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCTAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCt      <  2:2591136/100‑1 (MQ=255)
                                                                                             cAATGGTTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCt      <  1:359257/99‑1 (MQ=255)
                                                                                              aaTGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTg    >  2:2722335/1‑100 (MQ=255)
                                                                                                tGGGTCGTGGGGCGAATGGGTGGCAAAGCTTCCGCTACGTAGTGTTGCCGACGCTCAGTTCGGCGTTGGGGGCAGGAGGAATGCTGGCGTTTg         <  2:3831753/93‑1 (MQ=255)
                                                                                                   |                                                                                                
TCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTC  >  NC_000913/1515168‑1515363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: