Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,519,159 A→T 21.8% T45S (ACA→TCA)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,519,1590AT21.8% 240.9 / 65.7 115T45S (ACA→TCA) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (41/48);  new base T (14/11);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 120 total aligned reads displayed.

GTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGG  >  NC_000913/1519060‑1519256
                                                                                                   |                                                                                                 
gTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGca                                                                                                   >  1:143224/1‑100 (MQ=255)
 ttCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGcac                                                                                                  <  1:1731384/100‑1 (MQ=255)
  tCTGGCCTCGCGTGCACATGGCGCACCTGTTCCGTAGAATTTCCGTCTTGAAGAATATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGcaca                                                                                                 <  2:574798/100‑1 (MQ=255)
  tCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGcaca                                                                                                 >  1:1696409/1‑100 (MQ=255)
    tGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGt                                                                                               <  1:2159719/100‑1 (MQ=255)
     ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGCTGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCCcaca                                                                                                 >  2:3802676/1‑97 (MQ=255)
     ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGtt                                                                                              >  1:3005491/1‑100 (MQ=255)
     ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGtt                                                                                              >  1:2831790/1‑100 (MQ=255)
     ggCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGtt                                                                                              <  1:1476535/100‑1 (MQ=255)
        cTCGCGTCCCCATGGCGCACCTGTGCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTAt                                                                                           <  1:3780170/100‑1 (MQ=255)
        cTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTAt                                                                                           <  2:3086035/100‑1 (MQ=255)
        cTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTAt                                                                                           <  2:1060537/100‑1 (MQ=255)
          cgcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATTTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTAttt                                                                                         <  1:674947/100‑1 (MQ=255)
           gcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTg                                                                                        <  2:348661/100‑1 (MQ=255)
           gcgTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTg                                                                                        >  1:4088203/1‑100 (MQ=255)
             gTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTc                                                                                      <  1:1184733/100‑1 (MQ=255)
              tCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTcc                                                                                     >  1:120876/1‑100 (MQ=255)
              tCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTcc                                                                                     >  1:3632804/1‑100 (MQ=255)
               ccACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTccc                                                                                    <  2:3538846/100‑1 (MQ=255)
               ccACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTccc                                                                                    >  2:4066185/1‑100 (MQ=255)
                cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCATTTTATTTGTCCCt                                                                                   >  2:3347396/1‑100 (MQ=255)
                cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCt                                                                                   >  2:1139411/1‑100 (MQ=255)
                cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCt                                                                                   >  2:1047521/1‑100 (MQ=255)
                cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCt                                                                                   >  2:2119661/1‑100 (MQ=255)
                cacaTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCt                                                                                   <  2:985832/100‑1 (MQ=255)
                  caTGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTgg                                                                                 <  2:4121777/100‑1 (MQ=255)
                     ggCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGAcc                                                                              <  2:3699263/100‑1 (MQ=255)
                       cgcACCTGTTCCGTAGAATTTCCGTCTTGAAGAAGATGATCTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCg                                                                            <  1:1609388/100‑1 (MQ=255)
                       cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCg                                                                            <  1:2590556/100‑1 (MQ=255)
                       cgcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCg                                                                            >  2:3610653/1‑100 (MQ=255)
                        gcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGt                                                                           >  2:467579/1‑100 (MQ=255)
                        gcACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGt                                                                           <  2:1091222/100‑1 (MQ=255)
                        gcACCTGTTCCGGAGAATTTCCGTCTAGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGCGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGt                                                                           >  2:3294734/1‑100 (MQ=255)
                          aCCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGtat                                                                         <  1:1380562/100‑1 (MQ=255)
                          aCCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGtat                                                                         <  1:3026314/100‑1 (MQ=255)
                             tGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATg                                                                      <  2:1990567/100‑1 (MQ=255)
                                tCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCCCAGTTTATTTGTCCCTGGACCCGTATATGCGt                                                                   >  2:2935686/1‑100 (MQ=255)
                                tCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGt                                                                   <  2:2172994/100‑1 (MQ=255)
                                 ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTg                                                                  >  1:2603489/1‑100 (MQ=255)
                                 ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTg                                                                  <  2:2085389/100‑1 (MQ=255)
                                 ccGGAGAATTTCCGTCTTGAAGAAGATGATGTCACCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTg                                                                  <  1:3118924/100‑1 (MQ=255)
                                  cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTgg                                                                 <  1:586016/100‑1 (MQ=255)
                                  cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTgg                                                                 >  2:2641649/1‑100 (MQ=255)
                                  cGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTgg                                                                 >  1:2308526/1‑100 (MQ=255)
                                   ggAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGa                                                                >  1:228719/1‑100 (MQ=255)
                                   ggAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGa                                                                >  2:3520059/1‑100 (MQ=255)
                                    gagaATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGACGGACAGGTGTTACTGCGCACAGTTTATTTGTCGCTGGACCCGTATATGCGTGGAc                                                               >  2:1016790/1‑100 (MQ=255)
                                       aaTTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTa                                                            <  1:3868204/100‑1 (MQ=255)
                                       aaTTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTCGACCCGTATATGCGTGGACGTa                                                            <  2:2421790/100‑1 (MQ=255)
                                         tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTAt                                                           >  1:2879614/1‑99 (MQ=255)
                                         tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATg                                                          >  1:1152473/1‑100 (MQ=255)
                                         tttCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGAAAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTAt                                                           >  2:2548747/1‑99 (MQ=255)
                                           tCCGTCTTGAAGAATGGGATGTCGCCACACCGTGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAg                                                        <  1:768869/100‑1 (MQ=255)
                                               tCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGAt                                                    <  2:2640115/100‑1 (MQ=255)
                                                  tGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAg                                                 <  1:1221811/100‑1 (MQ=255)
                                                  tGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAg                                                 <  2:3455298/100‑1 (MQ=255)
                                                  tGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAg                                                 <  1:1826630/100‑1 (MQ=255)
                                                   gaagaaGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGc                                                <  1:142024/100‑1 (MQ=255)
                                                       aagaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATc                                            >  1:1256921/1‑100 (MQ=255)
                                                        agaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCt                                           >  1:1476060/1‑100 (MQ=255)
                                                        agaTGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCt                                           >  1:3059529/1‑100 (MQ=255)
                                                         gatgatGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCtt                                          >  1:1919155/1‑100 (MQ=255)
                                                         gatgatGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCtt                                          <  1:1632649/100‑1 (MQ=255)
                                                         gatgatGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCtt                                          <  2:2582175/100‑1 (MQ=255)
                                                           tgatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAt                                        >  1:2214233/1‑100 (MQ=255)
                                                            gatgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTAtt                                       >  2:3635279/1‑100 (MQ=255)
                                                             atgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTc                                      <  1:3610680/100‑1 (MQ=255)
                                                              tgTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCa                                     >  2:2262228/1‑100 (MQ=255)
                                                                    cacaCCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCt                               >  2:1266964/1‑100 (MQ=255)
                                                                    cacaCCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCt                               >  1:1675698/1‑100 (MQ=255)
                                                                      cacCGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGt                             >  1:2043134/1‑100 (MQ=255)
                                                                      cacCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGt                             <  1:3719303/100‑1 (MQ=255)
                                                                        ccGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGtg                            <  2:582834/99‑2 (MQ=255)
                                                                        ccGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTg                           >  2:759101/1‑100 (MQ=255)
                                                                        ccGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTg                           >  2:3098326/1‑100 (MQ=255)
                                                                        ccGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTg                           <  1:3268666/100‑1 (MQ=255)
                                                                         cGGGTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGa                          >  1:2751414/1‑100 (MQ=255)
                                                                           ggTGAAGGACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGata                        >  1:2410273/1‑100 (MQ=255)
                                                                               aaGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTgg                    <  1:816654/100‑1 (MQ=255)
                                                                                aGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggc                   <  1:4066216/100‑1 (MQ=255)
                                                                                 ggACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcg                  >  2:3563961/1‑100 (MQ=255)
                                                                                 ggACAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcg                  >  1:3515285/1‑100 (MQ=255)
                                                                                   aCAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTggcggc                <  1:4040328/100‑1 (MQ=255)
                                                                                    cAGGTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg               <  1:3832089/100‑1 (MQ=255)
                                                                                    cAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg               <  1:2950102/100‑1 (MQ=255)
                                                                                      ggTGTTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg             >  1:3919218/1‑100 (MQ=255)
                                                                                      ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGgcggcg               >  2:1507097/1‑98 (MQ=255)
                                                                                      ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg             >  1:4087165/1‑100 (MQ=255)
                                                                                      ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg             >  2:4069137/1‑100 (MQ=255)
                                                                                      ggTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg             >  2:1695596/1‑100 (MQ=255)
                                                                                       gtgtTACTGCGCTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTg             <  2:4066209/99‑1 (MQ=255)
                                                                                         gtTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATg          <  2:1565465/100‑1 (MQ=255)
                                                                                            aCTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGtn       <  1:2073672/100‑2 (MQ=255)
                                                                                            aCTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTc       >  1:3978691/1‑100 (MQ=255)
                                                                                            aCTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTc       >  1:958319/1‑100 (MQ=255)
                                                                                             cTGCGCACAGTTTATTTGTGCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGACTGTTGATATTGGCGGCGTGATGGTcg      >  2:3463410/1‑100 (MQ=255)
                                                                                               gcgcACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggc    <  1:606079/100‑1 (MQ=255)
                                                                                                cgcTCAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggcg   >  1:2917647/1‑100 (MQ=255)
                                                                                                 gcACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggcgg  >  2:428752/1‑100 (MQ=255)
                                                                                                 gcACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTcggcgg  >  1:1828390/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
GTTCTGGCCTCGCGTCCACATGGCGCACCTGTTCCGGAGAATTTCCGTCTTGAAGAAGATGATGTCGCCACACCGGGTGAAGGACAGGTGTTACTGCGCACAGTTTATTTGTCCCTGGACCCGTATATGCGTGGACGTATGAGCGATGAGCCATCTTATTCACCGCCTGTTGATATTGGCGGCGTGATGGTCGGCGG  >  NC_000913/1519060‑1519256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: