Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,533,713 A→C 15.5% F47V (TTT→GTT)  yddH ← flavin reductase like‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,533,7130AC15.5% 292.1 / 38.1 113F47V (TTT→GTT) yddHflavin reductase like‑protein
Reads supporting (aligned to +/- strand):  ref base A (50/43);  new base C (9/9);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACG  >  NC_000913/1533615‑1533812
                                                                                                  |                                                                                                   
aaCGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAAc                                                                                                    <  1:772841/100‑1 (MQ=255)
aaCGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAAc                                                                                                    <  1:3524209/100‑1 (MQ=255)
  cGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTc                                                                                                  >  2:868048/1‑100 (MQ=255)
   gCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTcc                                                                                                 >  1:987301/1‑100 (MQ=255)
      cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcg                                                                                              <  2:2036502/100‑2 (MQ=255)
      cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcg                                                                                              <  2:3810995/100‑2 (MQ=255)
      cGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCAcc                                                                                              <  2:2690751/100‑1 (MQ=255)
       gggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCg                                                                                             <  1:3497381/100‑1 (MQ=255)
       gggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCg                                                                                             <  1:1316432/100‑1 (MQ=255)
       gggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCg                                                                                             >  1:3115457/1‑100 (MQ=255)
        ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcg                                                                                            <  2:3450464/100‑4 (MQ=255)
        ggATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCgg                                                                                            <  1:1037310/100‑1 (MQ=255)
         gATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgt                                                                                           <  2:1505159/100‑5 (MQ=255)
         gATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgt                                                                                           <  1:497260/100‑5 (MQ=255)
           tAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCgg                                                                                            <  2:1495601/97‑1 (MQ=255)
           tAACGATGACAAAGTTACCGTTATGTGCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgtgg                                                                                         <  2:3754409/100‑7 (MQ=255)
            aaCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGTTTGAACCTCCAcgcgtggc                                                                                        <  1:2548446/100‑8 (MQ=38)
            aaCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCAtt                                                                                        >  2:967294/1‑100 (MQ=255)
             aCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTg                                                                                       <  2:615121/100‑1 (MQ=255)
             aCGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTg                                                                                       <  2:2029368/100‑1 (MQ=255)
              cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      >  1:3653675/1‑100 (MQ=255)
              cGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGa                                                                                      >  1:1252573/1‑100 (MQ=255)
                aTGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGAcc                                                                                    <  1:1361868/100‑1 (MQ=255)
                 tGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCa                                                                                   >  1:121053/1‑100 (MQ=255)
                   ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg                                                                                 >  2:3248809/1‑100 (MQ=255)
                   ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg                                                                                 >  2:3135238/1‑100 (MQ=255)
                   ccAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAgg                                                                                 <  2:216895/100‑1 (MQ=255)
                    cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCTCGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                <  2:2231456/100‑1 (MQ=255)
                    cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                >  1:1664602/1‑100 (MQ=255)
                    cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                <  1:22828/100‑1 (MQ=255)
                    cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                <  1:1231853/100‑1 (MQ=255)
                    cAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGc                                                                                >  1:2986027/1‑100 (MQ=255)
                          tACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGc                                                                          <  2:2637661/100‑1 (MQ=255)
                            ccGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCa                                                                        <  1:757807/100‑1 (MQ=255)
                             cGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCAt                                                                       <  1:389967/100‑1 (MQ=255)
                              gTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATa                                                                      >  1:3800683/1‑100 (MQ=255)
                                tATGGTCAATCGACTCCCTGGTCCATCTCGATTTATCTACCACCATCGCCACGCGTGGAGGTTCAAACTCCCCCGGCAGTGACCAGGCTGCATCCATAAt                                                                    <  2:1748078/100‑1 (MQ=255)
                                  tGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGt                                                                  >  2:3578265/1‑100 (MQ=255)
                                   gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCCCGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  2:1024218/1‑100 (MQ=255)
                                   gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  2:647603/1‑100 (MQ=255)
                                   gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGtt                                                                 >  1:1984362/1‑100 (MQ=255)
                                   gTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGt                                                                  >  2:647788/1‑99 (MQ=255)
                                    ttCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACTGTCATTGACCAGGCTGCAGCCATAATGTTa                                                                <  2:3460178/100‑1 (MQ=255)
                                     tcaatcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAc                                                               >  2:2550908/1‑100 (MQ=255)
                                      ccatcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTACTCCGGCATTGACCAGGCTGCAGCCATAATGTTa                                                                <  1:3961475/96‑1 (MQ=255)
                                      caatcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAcg                                                              >  1:332792/1‑100 (MQ=255)
                                       aatcaaCTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTAcgc                                                             >  1:1523494/1‑100 (MQ=255)
                                                   ggTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGc                                                 <  1:2028887/100‑1 (MQ=255)
                                                    gTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTCCGCCGCTGAGATTGCt                                                >  1:3918239/1‑100 (MQ=255)
                                                          gTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGa                                          >  1:3490314/1‑100 (MQ=255)
                                                           tCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCCTAATGTTACGCCGCTGGGATTGCTCATCGaa                                         >  1:2094027/1‑100 (MQ=255)
                                                           tCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaa                                         <  1:577839/100‑1 (MQ=255)
                                                           tCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGaa                                         >  1:866223/1‑100 (MQ=255)
                                                                ttATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTgg                                    <  1:1141979/100‑1 (MQ=255)
                                                                ttATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTgg                                    <  1:421653/100‑1 (MQ=255)
                                                                 tATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGt                                   >  2:3054907/1‑100 (MQ=255)
                                                                    cTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGAt                                >  1:1212049/1‑100 (MQ=255)
                                                                    cTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACACCGCTGGGATTGCTCATCGAAACTGGTGAt                                <  2:2988989/100‑1 (MQ=255)
                                                                     tACCACAATCGGCACGCGTGGAGGTTCAAGCGTGACTGGCATTGACCAGGCTGCAGCTATAATGTTACGCCGCGGGGATTGCTCATCGAAACTGGTGATc                               >  2:345873/1‑100 (MQ=255)
                                                                     tACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATc                               >  1:2269057/1‑100 (MQ=255)
                                                                     tACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATc                               >  2:4098332/1‑100 (MQ=255)
                                                                      aCCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCa                              >  2:3429765/1‑100 (MQ=255)
                                                                      aCCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCa                              >  1:3380476/1‑100 (MQ=255)
                                                                       ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  2:1137448/1‑100 (MQ=255)
                                                                       ccACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCaa                             >  1:3080661/1‑100 (MQ=255)
                                                                         acaATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGa                           >  2:2248567/1‑100 (MQ=255)
                                                                          caATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGAc                          <  2:4008858/100‑1 (MQ=255)
                                                                               gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg                     >  1:2304736/1‑100 (MQ=255)
                                                                               gCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTg                     <  1:3637488/100‑1 (MQ=255)
                                                                                ccACGCGTGGAGGTTCAACCTCCACCGGCATTGACCAGGCTGCAGCCCTAATGTTACGCCGCTGCGATTGCTCATCGAAACTGGTGATCAAGACCGTTgg                    >  2:3308265/1‑100 (MQ=255)
                                                                                ccACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTgg                    <  1:1867723/100‑1 (MQ=255)
                                                                                 cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATGATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   >  1:2540080/1‑100 (MQ=255)
                                                                                 cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   <  1:741238/100‑1 (MQ=255)
                                                                                 cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   <  2:732017/100‑1 (MQ=255)
                                                                                 cACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTggg                   <  2:1341582/100‑1 (MQ=255)
                                                                                  gccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGc                  >  1:3048971/5‑100 (MQ=255)
                                                                                   cgcgTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 <  1:1461847/100‑1 (MQ=255)
                                                                                   ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGCTCAAGACCGTTGGGc                  >  1:3364688/4‑99 (MQ=255)
                                                                                   ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  1:324209/4‑100 (MQ=255)
                                                                                   ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  2:30215/4‑100 (MQ=255)
                                                                                   ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  2:798047/4‑100 (MQ=255)
                                                                                   ccggTGGAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGcc                 >  1:2920396/4‑100 (MQ=255)
                                                                                   ccgaaggAGTTTGAACCTCCACCGGCATTGACCAGGCTGCAGAAATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTAATCAAGACAGTTGGGcc                 >  2:2253477/6‑100 (MQ=255)
                                                                                     cgTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCAt               >  2:1451802/1‑100 (MQ=255)
                                                                                       tGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGa             >  1:3121799/1‑100 (MQ=255)
                                                                                       tGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGa             <  1:3603349/100‑1 (MQ=255)
                                                                                          aGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAttt          <  1:2995459/100‑1 (MQ=255)
                                                                                          aGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGAttt          <  2:3164984/100‑1 (MQ=255)
                                                                                           ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         >  1:2967848/1‑100 (MQ=255)
                                                                                           ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         <  1:1473735/100‑1 (MQ=255)
                                                                                           ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         <  2:708713/100‑1 (MQ=255)
                                                                                           ggTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTa         >  2:1248300/1‑100 (MQ=255)
                                                                                            gTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTaa        >  1:2768244/1‑100 (MQ=255)
                                                                                             ttCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAAc       >  2:3924895/1‑100 (MQ=255)
                                                                                             ttCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAAc       >  2:22302/1‑100 (MQ=255)
                                                                                             ttCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAAc       >  1:3636020/1‑100 (MQ=255)
                                                                                              tCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACa      >  2:1793996/1‑100 (MQ=255)
                                                                                               cAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAg     <  1:3260415/100‑1 (MQ=255)
                                                                                               cAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAg     <  1:1450832/100‑1 (MQ=255)
                                                                                                  aCTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACg  <  2:1603732/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
AACGCCCGGGATAACGATGCCAAATTTACCGTTATGTTCAATCAACTCCCTGGTCCATGTCGATTTATCTACCACAATCGCCACGCGTGGAGGTTCAAACTCCACCGGCATTGACCAGGCTGCAGCCATAATGTTACGCCGCTGGGATTGCTCATCGAAACTGGTGATCAAGACCGTTGGGCCATGATTTAACAGACG  >  NC_000913/1533615‑1533812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: