Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,828 C→T 18.5% intergenic (‑4/‑197) slyA ← / → ydhI global transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,8280CT18.5% 302.4 / 49.3 134intergenic (‑4/‑197)slyA/ydhIglobal transcriptional regulator/DUF1656 family putative inner membrane efflux pump associated protein
Reads supporting (aligned to +/- strand):  ref base C (53/55);  new base T (12/13);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01

Only 100 of 137 total aligned reads displayed.

GGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCG  >  NC_000913/1720729‑1720927
                                                                                                   |                                                                                                   
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    <  2:448478/100‑1 (MQ=255)
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    >  2:3549068/1‑100 (MQ=255)
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    >  2:2099730/1‑100 (MQ=255)
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    <  2:1100975/100‑1 (MQ=255)
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    <  1:3877423/100‑1 (MQ=255)
 gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCa                                                                                                    <  1:1351375/100‑1 (MQ=255)
    tgtgTTAACTCCAGCGGTTTCAGGCCATGTTCTATCAGAGCACGCCATATTCGCACCAACCGTGCCATATCAGAACCTAGTGGCGATTCCAATTTTAtct                                                                                                 <  2:3302701/100‑1 (MQ=255)
     gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAtctc                                                                                                <  2:3661/100‑1 (MQ=255)
     gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCAtctc                                                                                                <  1:4130659/100‑1 (MQ=255)
      tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTcc                                                                                               >  1:3376769/1‑100 (MQ=255)
      tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTcc                                                                                               >  2:63127/1‑100 (MQ=255)
       gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCt                                                                                              >  2:3607761/1‑100 (MQ=255)
         tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTta                                                                                            >  1:3302444/1‑100 (MQ=255)
         tAACTCCAGCGGTTTCAGGCGATGATCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTcc                                                                                               <  2:1136053/97‑1 (MQ=255)
          aaCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat                                                                                           <  1:3371033/100‑1 (MQ=255)
          aaCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat                                                                                           >  2:3874307/1‑100 (MQ=255)
           aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtata                                                                                          >  1:3142860/1‑100 (MQ=255)
           aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtata                                                                                          <  2:2176793/100‑1 (MQ=255)
            cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa                                                                                         >  2:1054002/1‑100 (MQ=255)
              ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAtt                                                                                       >  1:375599/1‑100 (MQ=255)
              ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAtt                                                                                       >  2:544726/1‑100 (MQ=255)
              ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAtt                                                                                       >  1:1512424/1‑100 (MQ=255)
               cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa                                                                                      >  2:2334748/1‑100 (MQ=255)
                   ggTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCtt                                                                                  <  2:2587247/100‑1 (MQ=255)
                     tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGc                                                                                <  1:1415161/100‑1 (MQ=255)
                     tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGc                                                                                <  2:3138427/100‑1 (MQ=255)
                      ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt                                                                               <  1:1597558/100‑1 (MQ=255)
                      ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt                                                                               >  1:3782390/1‑100 (MQ=255)
                         aGGCGATGGTCTATCACAGCACGCCATATGCGCACCAACCGTACCATATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg                                                                            <  2:2018911/100‑1 (MQ=255)
                          ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGa                                                                           <  1:1666991/100‑1 (MQ=255)
                          ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGa                                                                           >  1:1629542/1‑100 (MQ=255)
                           gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGat                                                                          >  1:1132207/1‑100 (MQ=255)
                            cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata                                                                         <  1:3203715/100‑1 (MQ=255)
                             gATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGatat                                                                        <  2:3212769/100‑1 (MQ=255)
                               tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                      <  1:3607788/100‑1 (MQ=255)
                               tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta                                                                      <  1:2365547/100‑1 (MQ=255)
                                  tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGc                                                                   <  2:2108823/100‑1 (MQ=255)
                                  tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGc                                                                   <  1:919371/100‑1 (MQ=255)
                                   cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg                                                                  <  1:2659384/100‑1 (MQ=255)
                                    tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCgg                                                                 <  1:2682559/100‑1 (MQ=255)
                                    tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCgg                                                                 >  2:1372430/1‑100 (MQ=255)
                                       cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTCTAATTAGCTTGCTAAGATATTATGCGGCtt                                                              >  2:1969943/1‑100 (MQ=255)
                                        agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt                                                              >  2:2009167/1‑99 (MQ=255)
                                        agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt                                                             >  1:718900/1‑100 (MQ=255)
                                           gCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg                                                          <  1:3229150/100‑1 (MQ=255)
                                             aCGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa                                                        <  2:3879125/100‑1 (MQ=255)
                                              cGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt                                                       >  2:3163220/1‑100 (MQ=255)
                                               gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa                                                      >  1:401633/1‑100 (MQ=255)
                                                 cATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCCTCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTCGAATAgt                                                    >  2:3963166/1‑100 (MQ=255)
                                                  atatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtg                                                   <  2:1161798/100‑1 (MQ=255)
                                                   tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt                                                  >  2:1300073/1‑100 (MQ=255)
                                                    atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt                                                  >  1:1150698/1‑99 (MQ=255)
                                                    atGCGCACCAACCGTACAAGATCAGAACCTAATGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAatgt                                                  >  2:1536485/1‑99 (MQ=255)
                                                     tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc                                                >  2:2546789/1‑100 (MQ=255)
                                                      gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca                                               <  2:703360/100‑1 (MQ=255)
                                                        gcACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc                                             <  1:4059455/100‑1 (MQ=255)
                                                         cACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca                                            >  1:2199944/1‑100 (MQ=255)
                                                           cctaCCGTGCCAGATCAGAAGCTAGTGGCGACTGCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGCGCAGCaa                                           >  2:2105831/4‑99 (MQ=255)
                                                           ccAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAt                                          >  1:1982080/1‑100 (MQ=255)
                                                             aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCTATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                        <  2:1295610/100‑1 (MQ=255)
                                                             aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                        <  1:838497/100‑1 (MQ=255)
                                                             aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg                                        <  2:1236134/100‑1 (MQ=255)
                                                               ccGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTa                                      <  1:544730/100‑1 (MQ=255)
                                                                 gTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTAtt                                    >  1:619727/1‑100 (MQ=255)
                                                                  tGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                                   <  2:3858042/100‑1 (MQ=255)
                                                                  tGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                                   >  2:1545356/1‑100 (MQ=255)
                                                                   gCCAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGc                                  <  1:3911685/100‑1 (MQ=255)
                                                                    gcAGATGAGCACCGAGTGTCGAATCCAATTTTACCACCTTATAATCAGTTTGCAAAAAGATTATGCGGCCTTTAGAATAGTGTGCAGCAATTGTa                                      >  2:1035503/2‑95 (MQ=255)
                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 <  2:3276595/100‑1 (MQ=255)
                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTg                                   <  2:2284558/98‑1 (MQ=255)
                                                                    ccAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCt                                 <  1:1609469/100‑1 (MQ=255)
                                                                     cAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTa                                <  1:662431/100‑1 (MQ=255)
                                                                      aGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaa                               >  2:497235/1‑100 (MQ=255)
                                                                       gATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaa                              <  1:3549095/100‑1 (MQ=255)
                                                                       gATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTaaa                              <  1:4143370/100‑1 (MQ=255)
                                                                         tCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAAc                            <  2:1629533/100‑1 (MQ=255)
                                                                          cAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACa                           <  1:1300080/100‑1 (MQ=255)
                                                                            gAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACaaa                         >  1:2107952/1‑100 (MQ=255)
                                                                             aaCCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAAt                        <  2:541733/100‑1 (MQ=255)
                                                                              aCCTAGTGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATg                       <  1:3661355/100‑1 (MQ=255)
                                                                                 tAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAt                    >  2:53149/1‑100 (MQ=255)
                                                                                 tAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAt                    >  2:3780887/1‑100 (MQ=255)
                                                                                 tAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTAt                    <  2:2885281/100‑1 (MQ=255)
                                                                                    tGGCGATTCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGACTAGTGTGCAGCAATTGTATTGCTCAAACAAATGTATtgc                 >  2:3274893/1‑100 (MQ=255)
                                                                                    tGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATtgc                 <  1:3083731/100‑1 (MQ=255)
                                                                                           tCCAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCAttt          <  2:3979292/100‑1 (MQ=255)
                                                                                            ccAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTg         >  1:3031192/1‑100 (MQ=255)
                                                                                            ccAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTg         >  2:2167195/1‑100 (MQ=255)
                                                                                            ccAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTg         >  1:2116373/1‑100 (MQ=255)
                                                                                             naaTTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATTTATTGCTGCATTTgg        >  2:4061619/2‑100 (MQ=255)
                                                                                             cAATTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCGTTTAGAATAGTGTGAAGCAATTGTATTGCTAACACAAATGTATTGCTGCATTTg         >  2:1415022/1‑99 (MQ=255)
                                                                                             cAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTgg        <  2:3699748/100‑1 (MQ=255)
                                                                                             cAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTgg        >  2:2070753/1‑100 (MQ=255)
                                                                                             cAACTTGATCTCGTGATACTTAGCCTGCTTAGATATTCTGGGGCTTTTAGGATTGTGTGCTGATAAAGTATTGCTAAATCAAATGTATTGCTGCa             >  2:2704698/1‑95 (MQ=255)
                                                                                              aaTTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGt       >  1:3964906/1‑100 (MQ=255)
                                                                                               aTTTTATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGtt      <  2:3067260/100‑1 (MQ=255)
                                                                                                tttCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTa     <  1:223734/100‑1 (MQ=255)
                                                                                                  tCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTAcc   >  1:2068441/1‑100 (MQ=255)
                                                                                                  tCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTAcc   >  2:1284542/1‑100 (MQ=255)
                                                                                                   cATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCg  <  1:3005784/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTGTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCG  >  NC_000913/1720729‑1720927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: