Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,841,079 C→A 73.2% A109S (GCG→TCG)  ynjF ← CDP‑alcohol phosphatidyltransferase family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,841,0790CA73.2% 255.6 / 116.8 148A109S (GCG→TCG) ynjFCDP‑alcohol phosphatidyltransferase family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base A (60/48);  total (81/67)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

GGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAG  >  NC_000913/1840982‑1841175
                                                                                                 |                                                                                                
gggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAAt                                                                                                <  1:2195125/100‑1 (MQ=255)
 ggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAtt                                                                                               <  2:835909/100‑1 (MQ=255)
 ggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAtt                                                                                               >  1:1235287/1‑100 (MQ=255)
 ggTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGGGCCGATAAACACGAACAACAACCAGCCGCCTGACAGCCCAt                                                                                                >  2:1192278/1‑99 (MQ=255)
  gTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGGCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCAttt                                                                                              >  1:362816/1‑100 (MQ=255)
  gTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAAttt                                                                                              >  1:942506/1‑100 (MQ=255)
   tgaTCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAAtttt                                                                                             <  2:2524457/98‑1 (MQ=255)
    tATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTg                                                                                            >  1:2494279/1‑100 (MQ=255)
      tCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCt                                                                                          >  1:2709109/1‑100 (MQ=255)
        aaTCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTcc                                                                                        <  1:3062267/100‑1 (MQ=255)
        aaTCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTcc                                                                                        <  2:2756432/100‑1 (MQ=255)
        aaTCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTcc                                                                                        >  2:4175102/1‑100 (MQ=255)
        aaTCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTcc                                                                                        >  1:2482269/1‑100 (MQ=255)
          tCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCgg                                                                                      <  2:4037894/100‑1 (MQ=255)
          tCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCAGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCgg                                                                                      >  1:1975103/1‑100 (MQ=255)
            tGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTg                                                                                    <  1:2248052/100‑1 (MQ=255)
             gATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgc                                                                                   >  2:1151856/1‑100 (MQ=255)
             gATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTgc                                                                                   <  2:2290887/100‑1 (MQ=255)
              aTGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTgca                                                                                  <  1:3354238/100‑1 (MQ=255)
               tGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTgcag                                                                                 >  1:1136114/1‑100 (MQ=255)
                gTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTgcagc                                                                                <  1:919147/100‑1 (MQ=255)
                gTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTgcagc                                                                                >  1:3173033/1‑100 (MQ=255)
                   ttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAg                                                                             <  1:298557/100‑1 (MQ=255)
                   ttGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAg                                                                             >  2:3595838/1‑100 (MQ=255)
                     gctgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAACAAGaa                                                                           >  2:2997573/1‑100 (MQ=255)
                       tgcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCCACAGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATa                                                                         >  2:3949464/1‑100 (MQ=255)
                        gcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATaa                                                                        >  1:236770/1‑100 (MQ=255)
                        gcCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATaa                                                                        >  1:1835006/1‑100 (MQ=255)
                          cAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAg                                                                      <  1:799599/100‑1 (MQ=255)
                          cAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAg                                                                      >  1:1298195/1‑100 (MQ=255)
                          cAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAg                                                                      <  1:1126410/100‑1 (MQ=255)
                           aGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGc                                                                     <  1:4075762/100‑1 (MQ=255)
                               cagcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGTTCCTTTTCTGCAAGAATAAACCCaaa                                                                 >  2:492535/1‑100 (MQ=255)
                               cagcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCaaa                                                                 >  2:1458975/1‑100 (MQ=255)
                                agcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAAc                                                                >  1:841299/1‑100 (MQ=255)
                                 gcaAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACg                                                               >  1:522679/1‑100 (MQ=255)
                                   aaaGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGc                                                             <  1:3614550/100‑1 (MQ=255)
                                   aaaGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGc                                                             >  2:3619043/1‑100 (MQ=255)
                                    aaGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCa                                                            >  1:1733459/1‑100 (MQ=255)
                                        cGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAg                                                        >  1:4003153/1‑100 (MQ=255)
                                          agaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcg                                                      <  1:2364746/100‑1 (MQ=255)
                                           gaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAgcgc                                                     >  1:3779888/1‑100 (MQ=255)
                                           gaAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGATCCGGTGCAGCAAGAATAAAACCAAACAGCACCAgcgc                                                     >  2:3648801/1‑100 (MQ=255)
                                              aaCTGCTGCCCGTGCCGATAAACGCAAACAACAACCGGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTa                                                  <  2:940473/100‑1 (MQ=255)
                                                ctgctgCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTaaa                                                <  1:3336604/100‑1 (MQ=255)
                                                   ctgcCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAaga                                             <  1:2974151/100‑1 (MQ=255)
                                                    tgcCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAagag                                            >  2:1220106/1‑100 (MQ=255)
                                                    tgcCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAagag                                            <  1:3747396/100‑1 (MQ=255)
                                                      cccGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaa                                          >  1:2527474/1‑100 (MQ=255)
                                                        cGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaaaa                                        >  1:92404/1‑100 (MQ=255)
                                                        cGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGaaaa                                        >  1:1218576/1‑100 (MQ=255)
                                                        cGTGCCGATAAACGCAAACAAAAAACAACCGCATACCAGAGAATTTTGCACCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGAGTAAAAAAGaaaa                                        >  2:1123608/1‑100 (MQ=255)
                                                          tGCCGATAAACGCAAACGACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATc                                      <  1:2562014/100‑1 (MQ=255)
                                                          tGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATc                                      >  2:2060307/1‑100 (MQ=255)
                                                            ccGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCga                                    >  1:999425/1‑100 (MQ=255)
                                                            ccGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCga                                    >  1:1530600/1‑100 (MQ=255)
                                                             cGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgag                                   <  1:1075633/100‑1 (MQ=255)
                                                             cGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgag                                   <  1:2060317/100‑1 (MQ=255)
                                                              gATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgaga                                  <  1:3106659/100‑1 (MQ=255)
                                                              gATAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATATTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgaga                                  >  2:2561044/1‑100 (MQ=255)
                                                               aTAAACGCAAACAACAACCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCgagag                                 <  2:236768/100‑1 (MQ=255)
                                                                 aaaCGGAAACAATAACCGGCTGTTTGTCAGTGAATTTTGCTGCGGTGCAGCAAGAATAAAGCCAAACGGTACCAGTGTGTAAAAGAGAAAATCgagag                                 <  2:2021606/98‑1 (MQ=255)
                                                                     gcgaacagcaacCAGCCGCCTGCTAGCGAATGCTGCTCCGGTGTAACAAGAATAAAGCCAAACGGCACCAGCTCGTAAAAGAGAAAATCGATAGAAat                             <  2:1030291/95‑1 (MQ=255)
                                                                     gCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAatat                           <  1:1220111/100‑1 (MQ=255)
                                                                      cAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATc                          >  1:2249731/1‑100 (MQ=255)
                                                                        aacgaaaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCaa                        <  2:1160197/94‑1 (MQ=255)
                                                                        aacaacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAAACGAGAGAAATATCaa                        >  1:4070881/1‑100 (MQ=255)
                                                                         acaacaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAGACGGCGCCAGCGCGTAAAAGAGAAAATCCAGCGAAATATc                          >  2:2481696/1‑97 (MQ=255)
                                                                          caacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGa                      >  1:1527858/1‑100 (MQ=255)
                                                                          caacaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGa                      >  1:3255896/1‑100 (MQ=255)
                                                                           aacaacCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGaa                     >  1:2931358/1‑100 (MQ=255)
                                                                            acaacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCaaagat                    >  1:1987251/1‑96 (MQ=255)
                                                                             caacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAg                   >  2:533245/1‑100 (MQ=255)
                                                                             caacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAg                   <  2:1717919/100‑1 (MQ=255)
                                                                             caacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAg                   >  1:3180035/1‑100 (MQ=255)
                                                                              aacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgc                  <  2:221511/100‑1 (MQ=255)
                                                                              aacCAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgc                  <  2:135324/100‑1 (MQ=255)
                                                                                ccAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccg                >  1:4084403/1‑100 (MQ=255)
                                                                                 cAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccgc               <  1:3830938/100‑1 (MQ=255)
                                                                                 cAGCCGCCTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccgc               <  1:3814025/100‑1 (MQ=255)
                                                                                  aGCCGCCTGCCAGCGAATGTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccgcc              <  2:2625276/100‑1 (MQ=255)
                                                                                   ggcgccTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGGACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAgccgc               <  2:3528459/96‑1 (MQ=255)
                                                                                    ccgccTGCCACCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGGGATAATCGAGAGAAATATCAAGAAAGccgcccg            <  2:2739626/100‑1 (MQ=255)
                                                                                     cgccTGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGccgcccgc           >  1:2712736/1‑100 (MQ=255)
                                                                                         tGCCAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCg       <  1:1601374/100‑1 (MQ=255)
                                                                                          gCCAACGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCgg      <  2:2019714/100‑1 (MQ=255)
                                                                                           ccAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt     >  2:1504424/1‑100 (MQ=255)
                                                                                           ccAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt     >  2:2966280/1‑100 (MQ=255)
                                                                                           ccAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt     >  1:3491438/1‑100 (MQ=255)
                                                                                           ccAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt     >  2:1871559/1‑100 (MQ=255)
                                                                                            cAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGt     <  2:4070850/99‑1 (MQ=255)
                                                                                            cAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTa    >  2:2172364/1‑100 (MQ=255)
                                                                                            cAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTa    <  1:1328346/100‑1 (MQ=255)
                                                                                            cAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTa    >  2:2108039/1‑100 (MQ=255)
                                                                                            cAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTa    >  1:1163927/1‑100 (MQ=255)
                                                                                            cAGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATAGCAAGAAAGCCGCCCGCGTCgg      <  1:2874729/98‑1 (MQ=255)
                                                                                             aGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTaa   <  1:651532/100‑1 (MQ=255)
                                                                                             aGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTaa   >  2:128986/1‑100 (MQ=255)
                                                                                             aGCGAATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTaa   >  1:3234744/1‑100 (MQ=255)
                                                                                              gcgaATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAg  <  1:1871569/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTGCTCCGGTGCAGCAAGAATAAAGCCAAACGGCACCAGCGCGTAAAAGAGAAAATCGAGAGAAATATCAAGAAAGCCGCCCGCGTCGGTAAG  >  NC_000913/1840982‑1841175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑